36 results about "Antigen capture" patented technology

Described herein are methods and kits for the detection of platelet-activating pathogenic antibodies in samples from patients suspected of having platelet-activating antibodies or non-HIT anti-PF4 antibody mediated syndromes using platelets and polyvinyl sulfonate or salts thereof. Upon platelet activation, thrombospondin-1 (TSP1), a platelet granule protein, is released and can be detected using a TSP1 antigen capture enzyme linked immunosorbent assay (ELISA) to accurately measure platelet activation.

The invention provides a double-antibody directionally-coupled FET (Field Effect Transistor) biosensor and a preparation method thereof, and belongs to the technical field of biosensors. The FET biosensor adopts a reference electrode to provide grid voltage, a buffer solution is a conductive electrolyte, the FET biosensor comprises a field effect transistor, a capture antibody and a detection antibody, the field effect transistor is composed of a source electrode, a drain electrode and a channel between the source electrode and the drain electrode, the bottom layer of the channel is an indium oxide thin film layer, the indium oxide thin film layer is modified with amino, and the detection antibody is a detection antibody. An amino-modified channel is obtained; carrying out passivation treatment on the source electrode and the drain electrode by adopting a mercaptan passivator; and cross-linking and fixing the capture antibody and the detection antibody on the channel modified with the amino group through a cross-linking fixing agent to obtain the FET biosensor. The two antibodies are combined according to different epitopes of antigens to form a sandwich structure, and double recognition of the antibodies on target antigens is utilized to enhance antigen capture efficiency and signal enrichment, so that a double-site recognition mode is formed, and low-concentration antigen detection is realized.

The invention discloses a surface drug-loaded multifunctional engineering probiotic with antigen capture capability, a preparation method and application, the strain takes non-pathogenic probiotic as an original strain, after the engineering probiotic expressing protein stimulating immune cell development or differentiation is constructed, the surface of the engineering probiotic is modified with maleimide functional groups and drug-loaded nano-particles, and the surface drug-loaded multifunctional engineering probiotic with antigen capture capability is obtained. Therefore, the compound is a multifunctional bacterium with antigen capture, drug delivery and anti-tumor effects at the same time, and the effect of effectively promoting antigen tumors and far-end tumors through multifunction synergism is achieved. Meanwhile, the drug-loaded nanoparticles loaded on the surface of the engineering probiotics can form a slow-release carrier, so that the onset time of the drug is prolonged, and the bioavailability of the drug is improved; the ICD effect is induced, and the conventional cytotoxic effect is converted into the immune activation effect, so that the systemic toxicity caused by the application of large-dose chemotherapeutic drugs is avoided, and the immune activation function of bacteria is retained. The engineering probiotic preparation process is simple and efficient, the whole process is synthesized in a water phase, and the safety is high.

The invention discloses a rapid qualitative detection test strip based on a capture method and a competition method. The test strip comprises a long-strip-shaped sample pad, a combination pad, an NC membrane, absorbent paper and a PVC backboard, the conjugate pad is coated with a marked and sealed compound and a quality control material for marking a tracer, and the NC membrane is sequentially provided with a capture line, a detection line and a quality control line; the compound is obtained by carrying out blocking reaction on a competitive substance for marking a tracer and a competitive substance; the capture line coats a capture substance and is used for capturing a competed substance; the detection line is coated with a competitive substance and reacts with the competitive substance marking the tracer; the quality control line is coated with a quality control second antibody; the competed substance is an antigen, and the capturing substance is an antibody. A capture method and a competition method are combined, through combined use of a capture line and a detection line, color development inhibition through the competition method is converted into color development judgment, qualitative detection is achieved, a quality control system is introduced into a detection system, the reaction process is monitored, and whether the detection process is effective or not is judged.

The invention discloses a nano vaccine for in-situ antigen capture and lymph node targeted delivery as well as a preparation method and application of the nano vaccine. The nano vaccine comprises a polymer nano assembly and a drug, wherein the polymer nano assembly comprises an amphiphilic polymer and cationic liposome co-assembly; the amphiphilic polymer comprises a polyethylene glycol-polyphosphate segmented copolymer; the medicine comprises an immunologic adjuvant. The nano vaccine provided by the invention not only can be used for in-situ capture of tumor antigens, but also can carry antigens and immune agonists and deliver the antigens and immune agonists to tumor drainage lymph nodes in a targeted manner by utilizing the advantage of small size, so that the maturation of dendritic cells is promoted, the mature dendritic cells process the antigens and present the antigens to T cells, and CD8 + T cells are activated; and a strong anti-tumor immune response is induced.

The invention discloses lateral flow immunochromatography test paper based on phenylboronic acid directional coupling antibody mediation and a detection method of the lateral flow immunochromatography test paper. According to the lateral flow immunochromatography test paper, the phenylboronic acid directional coupling antibody is adopted, the detection sensitivity of LFIA is improved, specific coupling is conducted on the Fc segment of the antibody, the antigen capture rate is increased, and signal amplification is achieved; meanwhile, the nano-particles are combined, and the detection line is colored by utilizing the color of the nano-particles, so that the portability and the sensitivity of the whole detection are greatly improved; the detection method based on the test paper can realize qualitative analysis on a target object by observing the color depth on the detection line with naked eyes or quantitative analysis on the content of the target object by measuring the RGB value of the detection line, has the advantages of strong specificity on the target object, high sensitivity, convenience in use, simple and rapid detection process and safe and reliable detection result, and has a great application prospect.

This invention belongs to the field of biomedicine and specifically relates to an antigen-capturing and mannose receptor-targeting nanodrug, its preparation method, and its application. This nanodrug can capture tumor-associated antigens released after thermal ablation of cancer cells and, in combination with an FTO inhibitor, deliver them to tumor-infiltrating dendritic cells. After thermal ablation, the synergistic effect of the nanodrug and immune checkpoint blockade therapy inhibits distal tumor growth and improves long-term survival.

The invention discloses an oxidized low-density lipoprotein detection method based on a stabilizer and a blocking agent, and relates to the field of high-molecular compounds. The method comprises the following steps: copolymerizing a hydrophilic monomer, a hydrophobic monomer and an antioxidant functional group-containing monomer to form a multifunctional polymer stabilizer, representing the structure and performance of the multifunctional polymer stabilizer by adopting a nuclear magnetic resonance spectroscopy, a gel permeation chromatography and a dynamic light scattering method, and simulating and optimizing the monomer composition through molecular dynamics; preparing a sample reaction solution on the basis of a stabilizer; the method comprises the following steps: collecting a serum sample, and preparing a sample for detecting the oxidized low-density lipoprotein through stabilizing treatment of a sample reaction solution; a latex microsphere-labeled oxidized low-density lipoprotein monoclonal antibody is adopted as an antigen trapping agent and is subjected to incubation reaction with a sample, the difference value of absorbance values before and after the reaction is calculated, and the oxidized low-density lipoprotein concentration is calculated according to the standard curve. The stability of the ox-LDL sample can be improved, and the ox-LDL sample can also be used as an efficient blocking agent to reduce non-specific binding.

The invention relates to the technical field of detection, and relates to a single-molecule protein detection method based on a microarray chip and a chemiluminescence immunoassay technology. An antigen-antibody complex reaction solution is randomly segmented into tens of thousands of microarray holes with the single-hole volume at the pL level by utilizing a microarray chip containing 0.5-200 thousands of micropores with the diameter of 5-30 [mu] m. Each micropore is used as an independent reaction unit for detection and comprises one or zero antigen-antibody compounds, a chromogenic substrate or exciting light is added to collect signal data in all the micropores, and the number of all the antigen-antibody compounds in a reaction system is directly and quantitatively detected according to a Poisson distribution calculation formula. And calculating the concentration of the antigen captured in the microarray chip. The method disclosed by the invention has the advantages of small background signal interference, high sensitivity, specificity, accuracy and precision, simplicity and rapidness in operation, low cost and obvious advantages in the technical field of single molecule detection, and the lower limit of detection reaches fg / ml.

Compositions comprising migratory type 1 conventional dendritic cells in combination with a positively charged antigen capturing nanoparticle comprising a hydrophobic surface and use of the nanoparticle and dendritic cells optionally in combination with an immune checkpoint inhibitor in methods of treating cancer or preventing or reducing the risk of reoccurrence of a cancer in a subject.

The invention discloses a two-dimensional black phosphorus hybrid nano-drug based on RC48 protein modification as well as a preparation method and application of the two-dimensional black phosphorus hybrid nano-drug. According to the medicine, a metabolizable two-dimensional black phosphorus quantum sheet inorganic carrier is modified by amino polyethylene glycol monomethyl ether, so that the colloidal stability and the biocompatibility are improved, and the antigen capturing capability of antigen presenting cells is enhanced. Furthermore, active targeting of HER2 positive tumors is realized through coupling with dicetumab, and meanwhile, an adjuvant, namely leximod, is loaded, so that immune cells are activated, and macrophages are induced to secrete M1 type cytokines so as to reverse tumor immunosuppression. According to the system, precise treatment is achieved through a targeted recognition-photo-thermal ablation-immune activation synergistic mechanism, the innovation is that RC48 and R848 are integrated on a photosensitive black phosphorus platform for the first time, the tumor microenvironment regulation and control capacity is remarkably enhanced while the mild reaction condition is guaranteed, and a new strategy is provided for targeted immune combined treatment of breast cancer.

The invention discloses a BVDV antigen capture ELISA method based on rCD46 and rE2 monoclonal antibodies and a kit thereof, an expression strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on July 24, 2024, and the preservation number is CGMCC NO: 31440; the monoclonal antibody hybridoma cell strain E2mAb is preserved in the China General Microbiological Culture Collection Center on October 9, 2024, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.46055. The monoclonal antibody hybridoma cell strain E2mAb is a monoclonal antibody hybridoma cell strain E2mAb. According to the present invention, the BVDV receptor rCD46 protein is adopted as the coating antigen, the HRP labeled specific rE2 monoclonal antibody is adopted as the detection antibody, the condition optimization is performed to establish the BVDV antigen capture ELISA detection method, and the BVDV antigen capture ELISA detection kit based on the receptor rCD46 and the BVDV rE2 monoclonal antibody is firstly assembled in China;

The invention discloses an infectious pancreatic necrosis virus monoclonal antibody and application thereof. The invention provides hybridoma cell strains IPNV-vp2-178-2A7 (CCTCC (China Center For Type Culture Collection) NO: C2024368) and IPNV-vp2-380-1G12 (CCTCC NO: C2024369) as well as two monoclonal antibodies secreted by the two hybridoma cell strains. According to the invention, two monoclonal antibodies are utilized to establish an antigen capture ELISA detection method aiming at the VP2 protein of the IPNV virus, and the effect of the antigen capture ELISA detection method is evaluated to find that the antigen capture ELISA detection method can specifically, rapidly and sensitively detect the infectious pancreatic necrosis virus.

The invention discloses a BVDV antigen capture ELISA method based on rCD46 and rE2 monoclonal antibodies and a kit thereof, an expression strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on July 24, 2024, and the preservation number is CGMCC NO: 31440; the monoclonal antibody hybridoma cell strain E2mAb is preserved in the China General Microbiological Culture Collection Center on October 9, 2024, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.46055. The monoclonal antibody hybridoma cell strain E2mAb is a monoclonal antibody hybridoma cell strain E2mAb. According to the present invention, the BVDV receptor rCD46 protein is adopted as the coating antigen, the HRP labeled specific rE2 monoclonal antibody is adopted as the detection antibody, the condition optimization is performed to establish the BVDV antigen capture ELISA detection method, and the BVDV antigen capture ELISA detection kit based on the receptor rCD46 and the BVDV rE2 monoclonal antibody is firstly assembled in China;

The present application relates to the technical field of tumor immunotherapy. The present application provides a responsive antigen capture nano platform, a preparation method and application thereof. The product of the present application is applied by systemic administration such as intravenous injection; can specifically respond to peroxynitrite in the tumor microenvironment, realize the precise activation of the tumor site; can covalently capture tumor-related antigens through high efficiency, deliver the antigens to antigen presenting cells, so as to enhance the anti-tumor immune response; combined with photodynamic therapy can significantly inhibit tumor growth.

The present invention provides novel antigen capture nanoparticles (ACNPs) and pharmaceutical formulations containing such ACNPs. Also provided herein are methods for preparing ACNP formulations, and methods for treating a disease, such as cancer, in a subject with ACPN or a pharmaceutical formulation containing ACPN.

The present invention discloses an antibody combination for detecting mycobacterial lipoarabinomannan, its application, and a kit, relating to the field of biological detection. The inventors of this application screened and identified various sample types, identifying capture antibodies suitable for LAM antigen capture and detection antibodies suitable for LAM antigen detection. This resulted in a superior antibody combination for LAM antigen detection, which exhibits high sensitivity and specificity and can be used to detect LAM antigens in purified LAM, standard strains of Mycobacterium tuberculosis, clinical isolates, pleural effusions, plasma, and other bodily fluids. This provides a new antibody combination and method for LAM antigen detection, as well as a new method and kit for the auxiliary diagnosis of active tuberculosis.

The present invention discloses a biomimetic inorganic nanoparticle with antigen capture capability and a preparation method thereof. The biomimetic inorganic nanoparticle comprises a bacterial outer membrane vesicle, wherein the bacterial outer membrane vesicle is coated with Prussian blue-manganese dioxide inorganic nanoparticles, the vesicle wall of the bacterial outer membrane vesicle is embedded with a photosensitizer Ce6, and the outer surface of the vesicle wall of the bacterial outer membrane vesicle is modified with maleimide. After the nanomaterial is injected into the tumor, MnO2 catalyzes H2O2 to produce O2 to solve the problem of hypoxia at the tumor site. PB and Ce6 respectively play a PTT / PDT synergistic role in killing tumor cells, inducing cell immunogenic death and releasing tumor antigens. Mal captures the released antigens by forming a thioether bond. In addition, OMV contains danger signals derived from the bacterial outer membrane, stimulates dendritic cells to mature, and stimulates a strong tumor immune response. The above mechanism can synergistically and efficiently kill tumors and prevent their metastasis and recurrence, thereby achieving efficient treatment of colorectal cancer.

The invention belongs to the technical field of biological detection, and discloses an avian leukosis virus detection kit based on a magnetic particle chemiluminescence method and application thereof. The kit comprises a liquid A, a liquid B, a liquid C, a liquid D calibration product and a quality control product, the liquid A comprises magnetic particles coated with an avian leukosis virus antibody, and the liquid B comprises an acridinium ester labeled avian leukosis virus antibody. According to the kit disclosed by the invention, the avian leukosis virus antibody is coated on the magnetic particles, and the magnetic particles have a relatively large surface area, so that the antigen capture efficiency is greatly improved, and the detection specificity and sensitivity are enhanced. In addition, acridinium ester is adopted for labeling, the cost is low, enzyme catalysis is not needed, the influence of environment temperature and humidity on the reaction is small, luminescence is stable, the luminescence efficiency is high, background luminescence is low, the signal-to-noise ratio is high, the detection time is short, the detection efficiency and stability are greatly improved, and rapid detection of large-scale samples in production is facilitated.

The invention discloses an intelligent artificial bionic DC cell nanoparticle nDC-M and a preparation method, application and a detection method thereof. The nano-particle is of a core-shell structure, the inner core is a hypoxia response type nano-micelle of a sound-sensitive agent SP8 and IL-2 for activating T cells to be mature, the outer layer is coated with a mature DC cell membrane, and the nano-particle has the immune recognition characteristic and the treatment function. In a tumor hypoxic microenvironment, the micelle specifically releases SP8 and IL-2; sP8 generates ROS through ultrasonic excitation, tumor cell immunogenicity death is induced, and antigens are released; iL-2 activates proliferation and functions of tumor infiltration lymphocytes; the outer DC cell membrane and the antigen capture functional group enhance the collection and activation of immune cells through surface antigen presentation, and amplify the anti-tumor immune response. By combining sonodynamic therapy and immunoregulation, tumor growth is inhibited, metastatic load is reduced, good biocompatibility and safety are achieved, and a novel bionic delivery system is provided for metastatic tumor immune combined therapy.

The invention discloses a biological immunomagnetic bead-based broad-spectrum enrichment method before microbiological detection, which comprises the following steps: preparing a plurality of different types of sample immunomagnetic beads, and modifying the surface of each sample immunomagnetic bead with an antibody or ligand corresponding to each bacterial genus; mixing different types of sample immunomagnetic beads according to different proportions to obtain a plurality of mixed immunomagnetic bead reagents; mixing each mixed immunomagnetic bead reagent with a biological sample containing an antigen, respectively incubating, and combining the immunomagnetic beads with the antigen; after the immunomagnetic beads combined with the antigens are separated from the sample, the antigen capture rate under each proportion is recorded to determine the optimal magnetic bead proportion; and preparing a magnetic bead reagent according to the optimal magnetic bead ratio so as to carry out broad-spectrum enrichment on bacteria. According to the biological immunomagnetic bead-based broad-spectrum enrichment method before microbiological detection disclosed by the invention, the ratio of different specific immunomagnetic beads is finely regulated and controlled, so that the method not only improves the capture efficiency and purity of bacteria, but also realizes broad-spectrum enrichment of various bacteria.

The invention relates to the technical field of biological detection, in particular to a lung cancer immunofluorescence detection kit and an immunofluorescence staining method, and the lung cancer immunofluorescence detection kit comprises a functionalized glass slide, a fluorescence labeling probe group, a confining liquid, a washing buffer solution and a sample diluent. According to the method, multi-target synchronous detection is realized through sample pretreatment, antigen capture, non-specific binding site closing, fluorescence labeling and signal detection. By optimizing the reagent formula and the operation process, the detection efficiency, the sensitivity and the result reliability are remarkably improved, and the clinical requirements for efficient and accurate lung cancer diagnosis are met.

The application discloses a BVDV antigen capture ELISA method and kit based on rCD46 and rE2 monoclonal antibodies, and the expression strain is preserved in the China General Microbiological Culture Collection Center on July 24, 2024, with a preservation number of CGMCC NO: 31440; the monoclonal antibody hybridoma cell strain E2mAb strain is preserved in the China General Microbiological Culture Collection Center on October 09, 2024, with a preservation number of CGMCC NO: 46055. The application establishes a BVDV antigen capture ELISA detection method by using a BVDV receptor rCD46 protein as a coating antigen and using HRP-labeled specific rE2 monoclonal antibodies as detection antibodies, and domestic BVDV antigen capture ELISA detection kits based on the receptor rCD46 and the BVDV rE2 monoclonal antibodies are assembled for the first time.

A protein scaffold capable of carrying radioactive signals and application thereof in antibody detection are provided. The present disclosure successfully finds out a protein scaffold R12 capable of carrying radioactive signals, which is used for assisting part of antigens to capture corresponding antibodies; and because the method is used for carrying signals and eliminating more severe screening conditions of cross reaction in the screening process, the method is not limited to the method in application, and the method can be directly used for a technical platform which only needs to eliminate the cross reaction.

The invention relates to the technical field of medical treatment, in particular to dual-function nano-particles with capturing and targeting functions and a preparation method and application of the dual-function nano-particles. The preparation method comprises the steps that a PEG-PCL polymer and N-(2-ethoxyl) maleimide are dissolved in dichloromethane; then dissolving 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC.HCl) and 4-dimethylaminopyridine in the mixture, washing and concentrating to obtain PCL-PEG-Mal (polycaprolactone-polyethylene glycol-Mal); the preparation method comprises the following steps: dissolving COOH-PEG-PCL in N, N-dimethylformamide, then sequentially adding N-hydroxysuccinimide NHS and EDC.HCl, then adding a D-mannosamine salt solution, and carrying out freeze-drying to obtain a final product Mannose-PEG-PCL; and then obtaining the blank micelle freeze-dried powder drug-loaded nano-micelle. Therefore, the multifunctional nano-micelle with better biocompatibility can be provided, the antigen capture and antigen presentation effects are realized at the same time, the nano-particle can enable the antigen and the medicine to be effectively and jointly delivered to the DC in time and space, and strong and accurate immune tolerance is generated by inducing the antigen-specific Treg.

The invention provides a micro-fluidic chip for combined detection of hepatitis A / hepatitis E virus IgM antibody and application thereof, the micro-fluidic chip comprises a substrate and a cover plate pressed on the substrate, the substrate and the cover plate enclose to form a micro-channel, the micro-channel is provided with a marking area and a detection area, and the marking area and the detection area are arranged on the substrate. The marking area comprises a first marking area arranged on the lower surface of the cover plate and a second marking area arranged on the upper surface of the substrate, and the detection area comprises an HEV-IgM antibody detection area arranged on the lower surface of the cover plate and an HAV-IgM antibody detection area arranged on the upper surface of the substrate. An IgG antibody capture area is arranged on the upper surface of the substrate corresponding to the position right below the sample injection hole, the IgG antibody capture area is provided with a magnetic microsphere-anti-human IgG antibody, the first marking area is provided with a marked HEV antigen, the second marking area is provided with a marked anti-human IgM antibody, the HEV-IgM antibody detection area is provided with an anti-human IgM antibody, and the HAV-IgM antibody detection area is provided with an HAV antigen. A capture method and an indirect method are combined to realize joint detection of the IgM antibodies related to the two viruses.

The invention discloses an infectious hematopoietic necrosis virus monoclonal antibody, a hybridoma cell strain and application. The invention relates to the technical field of biology, and provides a complete set of hybridoma cell strain which is composed of a hybridoma cell strain IHNV-N-15-5H3 (CCTCC (China Center for Type Culture Collection) NO: C2024366) and a hybridoma cell strain IHNV-N-151-3A9 (CCTCC NO: C2024367). The invention also provides a complete set of monoclonal antibody secreted by the complete set of hybridoma cell strain. The antigen capture ELISA detection method aiming at the IHNV virus N protein is established by utilizing two monoclonal antibodies, and the effect of the antigen capture ELISA detection method is evaluated to find that the antigen capture ELISA detection method can be used for specifically, quickly and sensitively detecting the infectious hematopoietic necrosis virus.

This invention proposes a microfluidic chip for the combined detection of hepatitis A / hepatitis E virus IgM antibodies and its application. The chip includes a substrate and a cover plate pressed onto the substrate. The substrate and cover plate enclose a microchannel, which is provided with a labeling area and a detection area. The labeling area includes a first labeling area on the lower surface of the cover plate and a second labeling area on the upper surface of the substrate. The detection area includes an HEV-IgM antibody detection area on the lower surface of the cover plate and a HAV-IgM antibody detection area on the upper surface of the substrate. An IgG antibody capture area is provided on the upper surface of the substrate directly below the sample injection well. The IgG antibody capture area contains magnetic microspheres containing anti-human IgG antibodies. The first labeling area contains labeled HEV antigen, the second labeling area contains labeled anti-human IgM antibody, the HEV-IgM antibody detection area contains anti-human IgM antibody, and the HAV-IgM antibody detection area contains HAV antigen. The capture method and the indirect method are used in parallel to achieve the combined detection of IgM antibodies related to the two viruses.