28 results about "Antigen capture" patented technology

Described herein are methods and kits for the detection of platelet-activating pathogenic antibodies in samples from patients suspected of having platelet-activating antibodies or non-HIT anti-PF4 antibody mediated syndromes using platelets and polyvinyl sulfonate or salts thereof. Upon platelet activation, thrombospondin-1 (TSP1), a platelet granule protein, is released and can be detected using a TSP1 antigen capture enzyme linked immunosorbent assay (ELISA) to accurately measure platelet activation.

The invention provides a double-antibody directionally-coupled FET (Field Effect Transistor) biosensor and a preparation method thereof, and belongs to the technical field of biosensors. The FET biosensor adopts a reference electrode to provide grid voltage, a buffer solution is a conductive electrolyte, the FET biosensor comprises a field effect transistor, a capture antibody and a detection antibody, the field effect transistor is composed of a source electrode, a drain electrode and a channel between the source electrode and the drain electrode, the bottom layer of the channel is an indium oxide thin film layer, the indium oxide thin film layer is modified with amino, and the detection antibody is a detection antibody. An amino-modified channel is obtained; carrying out passivation treatment on the source electrode and the drain electrode by adopting a mercaptan passivator; and cross-linking and fixing the capture antibody and the detection antibody on the channel modified with the amino group through a cross-linking fixing agent to obtain the FET biosensor. The two antibodies are combined according to different epitopes of antigens to form a sandwich structure, and double recognition of the antibodies on target antigens is utilized to enhance antigen capture efficiency and signal enrichment, so that a double-site recognition mode is formed, and low-concentration antigen detection is realized.

The invention discloses a surface drug-loaded multifunctional engineering probiotic with antigen capture capability, a preparation method and application, the strain takes non-pathogenic probiotic as an original strain, after the engineering probiotic expressing protein stimulating immune cell development or differentiation is constructed, the surface of the engineering probiotic is modified with maleimide functional groups and drug-loaded nano-particles, and the surface drug-loaded multifunctional engineering probiotic with antigen capture capability is obtained. Therefore, the compound is a multifunctional bacterium with antigen capture, drug delivery and anti-tumor effects at the same time, and the effect of effectively promoting antigen tumors and far-end tumors through multifunction synergism is achieved. Meanwhile, the drug-loaded nanoparticles loaded on the surface of the engineering probiotics can form a slow-release carrier, so that the onset time of the drug is prolonged, and the bioavailability of the drug is improved; the ICD effect is induced, and the conventional cytotoxic effect is converted into the immune activation effect, so that the systemic toxicity caused by the application of large-dose chemotherapeutic drugs is avoided, and the immune activation function of bacteria is retained. The engineering probiotic preparation process is simple and efficient, the whole process is synthesized in a water phase, and the safety is high.

The invention discloses a rapid qualitative detection test strip based on a capture method and a competition method. The test strip comprises a long-strip-shaped sample pad, a combination pad, an NC membrane, absorbent paper and a PVC backboard, the conjugate pad is coated with a marked and sealed compound and a quality control material for marking a tracer, and the NC membrane is sequentially provided with a capture line, a detection line and a quality control line; the compound is obtained by carrying out blocking reaction on a competitive substance for marking a tracer and a competitive substance; the capture line coats a capture substance and is used for capturing a competed substance; the detection line is coated with a competitive substance and reacts with the competitive substance marking the tracer; the quality control line is coated with a quality control second antibody; the competed substance is an antigen, and the capturing substance is an antibody. A capture method and a competition method are combined, through combined use of a capture line and a detection line, color development inhibition through the competition method is converted into color development judgment, qualitative detection is achieved, a quality control system is introduced into a detection system, the reaction process is monitored, and whether the detection process is effective or not is judged.

This invention belongs to the field of biomedicine and specifically relates to an antigen-capturing and mannose receptor-targeting nanodrug, its preparation method, and its application. This nanodrug can capture tumor-associated antigens released after thermal ablation of cancer cells and, in combination with an FTO inhibitor, deliver them to tumor-infiltrating dendritic cells. After thermal ablation, the synergistic effect of the nanodrug and immune checkpoint blockade therapy inhibits distal tumor growth and improves long-term survival.

The invention relates to the technical field of detection, and relates to a single-molecule protein detection method based on a microarray chip and a chemiluminescence immunoassay technology. An antigen-antibody complex reaction solution is randomly segmented into tens of thousands of microarray holes with the single-hole volume at the pL level by utilizing a microarray chip containing 0.5-200 thousands of micropores with the diameter of 5-30 [mu] m. Each micropore is used as an independent reaction unit for detection and comprises one or zero antigen-antibody compounds, a chromogenic substrate or exciting light is added to collect signal data in all the micropores, and the number of all the antigen-antibody compounds in a reaction system is directly and quantitatively detected according to a Poisson distribution calculation formula. And calculating the concentration of the antigen captured in the microarray chip. The method disclosed by the invention has the advantages of small background signal interference, high sensitivity, specificity, accuracy and precision, simplicity and rapidness in operation, low cost and obvious advantages in the technical field of single molecule detection, and the lower limit of detection reaches fg / ml.

Compositions comprising migratory type 1 conventional dendritic cells in combination with a positively charged antigen capturing nanoparticle comprising a hydrophobic surface and use of the nanoparticle and dendritic cells optionally in combination with an immune checkpoint inhibitor in methods of treating cancer or preventing or reducing the risk of reoccurrence of a cancer in a subject.

The invention discloses a BVDV antigen capture ELISA method based on rCD46 and rE2 monoclonal antibodies and a kit thereof, an expression strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on July 24, 2024, and the preservation number is CGMCC NO: 31440; the monoclonal antibody hybridoma cell strain E2mAb is preserved in the China General Microbiological Culture Collection Center on October 9, 2024, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.46055. The monoclonal antibody hybridoma cell strain E2mAb is a monoclonal antibody hybridoma cell strain E2mAb. According to the present invention, the BVDV receptor rCD46 protein is adopted as the coating antigen, the HRP labeled specific rE2 monoclonal antibody is adopted as the detection antibody, the condition optimization is performed to establish the BVDV antigen capture ELISA detection method, and the BVDV antigen capture ELISA detection kit based on the receptor rCD46 and the BVDV rE2 monoclonal antibody is firstly assembled in China;

The invention discloses an infectious pancreatic necrosis virus monoclonal antibody and application thereof. The invention provides hybridoma cell strains IPNV-vp2-178-2A7 (CCTCC (China Center For Type Culture Collection) NO: C2024368) and IPNV-vp2-380-1G12 (CCTCC NO: C2024369) as well as two monoclonal antibodies secreted by the two hybridoma cell strains. According to the invention, two monoclonal antibodies are utilized to establish an antigen capture ELISA detection method aiming at the VP2 protein of the IPNV virus, and the effect of the antigen capture ELISA detection method is evaluated to find that the antigen capture ELISA detection method can specifically, rapidly and sensitively detect the infectious pancreatic necrosis virus.

The present application relates to the technical field of tumor immunotherapy. The present application provides a responsive antigen capture nano platform, a preparation method and application thereof. The product of the present application is applied by systemic administration such as intravenous injection; can specifically respond to peroxynitrite in the tumor microenvironment, realize the precise activation of the tumor site; can covalently capture tumor-related antigens through high efficiency, deliver the antigens to antigen presenting cells, so as to enhance the anti-tumor immune response; combined with photodynamic therapy can significantly inhibit tumor growth.

The present invention provides novel antigen capture nanoparticles (ACNPs) and pharmaceutical formulations containing such ACNPs. Also provided herein are methods for preparing ACNP formulations, and methods for treating a disease, such as cancer, in a subject with ACPN or a pharmaceutical formulation containing ACPN.

The present invention discloses an antibody combination for detecting mycobacterial lipoarabinomannan, its application, and a kit, relating to the field of biological detection. The inventors of this application screened and identified various sample types, identifying capture antibodies suitable for LAM antigen capture and detection antibodies suitable for LAM antigen detection. This resulted in a superior antibody combination for LAM antigen detection, which exhibits high sensitivity and specificity and can be used to detect LAM antigens in purified LAM, standard strains of Mycobacterium tuberculosis, clinical isolates, pleural effusions, plasma, and other bodily fluids. This provides a new antibody combination and method for LAM antigen detection, as well as a new method and kit for the auxiliary diagnosis of active tuberculosis.

The present invention discloses a biomimetic inorganic nanoparticle with antigen capture capability and a preparation method thereof. The biomimetic inorganic nanoparticle comprises a bacterial outer membrane vesicle, wherein the bacterial outer membrane vesicle is coated with Prussian blue-manganese dioxide inorganic nanoparticles, the vesicle wall of the bacterial outer membrane vesicle is embedded with a photosensitizer Ce6, and the outer surface of the vesicle wall of the bacterial outer membrane vesicle is modified with maleimide. After the nanomaterial is injected into the tumor, MnO2 catalyzes H2O2 to produce O2 to solve the problem of hypoxia at the tumor site. PB and Ce6 respectively play a PTT / PDT synergistic role in killing tumor cells, inducing cell immunogenic death and releasing tumor antigens. Mal captures the released antigens by forming a thioether bond. In addition, OMV contains danger signals derived from the bacterial outer membrane, stimulates dendritic cells to mature, and stimulates a strong tumor immune response. The above mechanism can synergistically and efficiently kill tumors and prevent their metastasis and recurrence, thereby achieving efficient treatment of colorectal cancer.

The invention discloses an intelligent artificial bionic DC cell nanoparticle nDC-M and a preparation method, application and a detection method thereof. The nano-particle is of a core-shell structure, the inner core is a hypoxia response type nano-micelle of a sound-sensitive agent SP8 and IL-2 for activating T cells to be mature, the outer layer is coated with a mature DC cell membrane, and the nano-particle has the immune recognition characteristic and the treatment function. In a tumor hypoxic microenvironment, the micelle specifically releases SP8 and IL-2; sP8 generates ROS through ultrasonic excitation, tumor cell immunogenicity death is induced, and antigens are released; iL-2 activates proliferation and functions of tumor infiltration lymphocytes; the outer DC cell membrane and the antigen capture functional group enhance the collection and activation of immune cells through surface antigen presentation, and amplify the anti-tumor immune response. By combining sonodynamic therapy and immunoregulation, tumor growth is inhibited, metastatic load is reduced, good biocompatibility and safety are achieved, and a novel bionic delivery system is provided for metastatic tumor immune combined therapy.

The invention relates to the technical field of biological detection, in particular to a lung cancer immunofluorescence detection kit and an immunofluorescence staining method, and the lung cancer immunofluorescence detection kit comprises a functionalized glass slide, a fluorescence labeling probe group, a confining liquid, a washing buffer solution and a sample diluent. According to the method, multi-target synchronous detection is realized through sample pretreatment, antigen capture, non-specific binding site closing, fluorescence labeling and signal detection. By optimizing the reagent formula and the operation process, the detection efficiency, the sensitivity and the result reliability are remarkably improved, and the clinical requirements for efficient and accurate lung cancer diagnosis are met.

The application discloses a BVDV antigen capture ELISA method and kit based on rCD46 and rE2 monoclonal antibodies, and the expression strain is preserved in the China General Microbiological Culture Collection Center on July 24, 2024, with a preservation number of CGMCC NO: 31440; the monoclonal antibody hybridoma cell strain E2mAb strain is preserved in the China General Microbiological Culture Collection Center on October 09, 2024, with a preservation number of CGMCC NO: 46055. The application establishes a BVDV antigen capture ELISA detection method by using a BVDV receptor rCD46 protein as a coating antigen and using HRP-labeled specific rE2 monoclonal antibodies as detection antibodies, and domestic BVDV antigen capture ELISA detection kits based on the receptor rCD46 and the BVDV rE2 monoclonal antibodies are assembled for the first time.

A protein scaffold capable of carrying radioactive signals and application thereof in antibody detection are provided. The present disclosure successfully finds out a protein scaffold R12 capable of carrying radioactive signals, which is used for assisting part of antigens to capture corresponding antibodies; and because the method is used for carrying signals and eliminating more severe screening conditions of cross reaction in the screening process, the method is not limited to the method in application, and the method can be directly used for a technical platform which only needs to eliminate the cross reaction.

The invention relates to the technical field of medical treatment, in particular to dual-function nano-particles with capturing and targeting functions and a preparation method and application of the dual-function nano-particles. The preparation method comprises the steps that a PEG-PCL polymer and N-(2-ethoxyl) maleimide are dissolved in dichloromethane; then dissolving 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC.HCl) and 4-dimethylaminopyridine in the mixture, washing and concentrating to obtain PCL-PEG-Mal (polycaprolactone-polyethylene glycol-Mal); the preparation method comprises the following steps: dissolving COOH-PEG-PCL in N, N-dimethylformamide, then sequentially adding N-hydroxysuccinimide NHS and EDC.HCl, then adding a D-mannosamine salt solution, and carrying out freeze-drying to obtain a final product Mannose-PEG-PCL; and then obtaining the blank micelle freeze-dried powder drug-loaded nano-micelle. Therefore, the multifunctional nano-micelle with better biocompatibility can be provided, the antigen capture and antigen presentation effects are realized at the same time, the nano-particle can enable the antigen and the medicine to be effectively and jointly delivered to the DC in time and space, and strong and accurate immune tolerance is generated by inducing the antigen-specific Treg.

The invention provides a micro-fluidic chip for combined detection of hepatitis A / hepatitis E virus IgM antibody and application thereof, the micro-fluidic chip comprises a substrate and a cover plate pressed on the substrate, the substrate and the cover plate enclose to form a micro-channel, the micro-channel is provided with a marking area and a detection area, and the marking area and the detection area are arranged on the substrate. The marking area comprises a first marking area arranged on the lower surface of the cover plate and a second marking area arranged on the upper surface of the substrate, and the detection area comprises an HEV-IgM antibody detection area arranged on the lower surface of the cover plate and an HAV-IgM antibody detection area arranged on the upper surface of the substrate. An IgG antibody capture area is arranged on the upper surface of the substrate corresponding to the position right below the sample injection hole, the IgG antibody capture area is provided with a magnetic microsphere-anti-human IgG antibody, the first marking area is provided with a marked HEV antigen, the second marking area is provided with a marked anti-human IgM antibody, the HEV-IgM antibody detection area is provided with an anti-human IgM antibody, and the HAV-IgM antibody detection area is provided with an HAV antigen. A capture method and an indirect method are combined to realize joint detection of the IgM antibodies related to the two viruses.

The invention discloses an infectious hematopoietic necrosis virus monoclonal antibody, a hybridoma cell strain and application. The invention relates to the technical field of biology, and provides a complete set of hybridoma cell strain which is composed of a hybridoma cell strain IHNV-N-15-5H3 (CCTCC (China Center for Type Culture Collection) NO: C2024366) and a hybridoma cell strain IHNV-N-151-3A9 (CCTCC NO: C2024367). The invention also provides a complete set of monoclonal antibody secreted by the complete set of hybridoma cell strain. The antigen capture ELISA detection method aiming at the IHNV virus N protein is established by utilizing two monoclonal antibodies, and the effect of the antigen capture ELISA detection method is evaluated to find that the antigen capture ELISA detection method can be used for specifically, quickly and sensitively detecting the infectious hematopoietic necrosis virus.

This invention proposes a microfluidic chip for the combined detection of hepatitis A / hepatitis E virus IgM antibodies and its application. The chip includes a substrate and a cover plate pressed onto the substrate. The substrate and cover plate enclose a microchannel, which is provided with a labeling area and a detection area. The labeling area includes a first labeling area on the lower surface of the cover plate and a second labeling area on the upper surface of the substrate. The detection area includes an HEV-IgM antibody detection area on the lower surface of the cover plate and a HAV-IgM antibody detection area on the upper surface of the substrate. An IgG antibody capture area is provided on the upper surface of the substrate directly below the sample injection well. The IgG antibody capture area contains magnetic microspheres containing anti-human IgG antibodies. The first labeling area contains labeled HEV antigen, the second labeling area contains labeled anti-human IgM antibody, the HEV-IgM antibody detection area contains anti-human IgM antibody, and the HAV-IgM antibody detection area contains HAV antigen. The capture method and the indirect method are used in parallel to achieve the combined detection of IgM antibodies related to the two viruses.

The present invention discloses a two-dimensional black phosphorus hybrid nanomedicine modified by RC48 protein, and its preparation method and application. The medicine uses a metabolizable two-dimensional black phosphorus quantum sheet as an inorganic carrier, and is modified by methoxypolyethylene glycol monoamine to improve the colloidal stability and biocompatibility, and enhance the antigen capture ability of antigen-presenting cells. Further conjugated with vedicitumumab to achieve active targeting of HER2-positive tumors, and at the same time loaded with adjuvant resiquimod to activate immune cells and induce macrophages to secrete M1-type cytokines to reverse tumor immunosuppression. This system realizes precise treatment through a "target recognition-photothermal ablation-immune activation" synergistic mechanism. Its innovation lies in the first integration of RC48 and R848 on a photosensitive black phosphorus platform, which significantly enhances the ability to regulate the tumor microenvironment while ensuring mild reaction conditions, providing a new strategy for targeted immunotherapy of breast cancer.

The present invention relates to an influenza vaccine composition based on a novel RNA having the dual functions of an immunopotentiator and antigen capture.

The present application discloses a method for detecting oxidized low-density lipoprotein based on a stabilizer and a blocker, which relates to the field of macromolecular compounds. The method includes: copolymerizing a hydrophilic monomer, a hydrophobic monomer and a monomer containing an antioxidant functional group to form a multifunctional polymer stabilizer, characterizing its structure and properties by nuclear magnetic resonance spectroscopy, gel permeation chromatography and dynamic light scattering method, and optimizing the monomer composition through molecular dynamics simulation; based on the stabilizer, preparing a sample reaction solution; collecting a serum sample, and preparing a sample for detecting oxidized low-density lipoprotein through the stabilization treatment of the sample reaction solution; using a monoclonal antibody against oxidized low-density lipoprotein labeled with latex microspheres as an antigen capturer, incubating and reacting with the sample, calculating the difference in absorbance values before and after the reaction, and calculating the concentration of oxidized low-density lipoprotein according to the standard curve. The present application can improve the stability of ox-LDL samples and can also be used as an efficient blocker to reduce non-specific binding.

This invention belongs to the field of biodetection technology, specifically relating to an immunoassay kit for early diagnosis of lung cancer and its preparation method. The immunoassay kit includes a chromatographic test strip, lyophilized capture microspheres, a chromogenic stop solution, a quantum dot labeling solution, and a Cell Lysis Buffer. The chromatographic test strip dynamically releases colloidal gold antibodies through a polyester fiber membrane for rapid visual screening. The lyophilized capture microspheres form a high-affinity antigen capture structure through the directional coupling of affinity peptides and dendritic binding domain proteins. The quantum dot labeling solution enables high-fidelity labeling of antibody activity. The chromogenic stop solution releases deep fluorescence and inhibits quenching effects, thus achieving the integration of material interface modification, molecular directional coupling, and signal amplification mechanisms, providing an efficient and reliable auxiliary detection method for early lung cancer screening.

An antibody-antigen capture ELISA-PCR test set for detection of a target protein comprises a first antibody-DNA conjugate having a first antibody which is configured to bind to a target protein; and a first DNA oligo linked to the first antibody; a second antibody-DNA conjugate having a second antibody which is configured to bind to the target protein; and a second DNA oligo linked to the second antibody; and multiple immobilized DNA fragments attached to a designated surface on their attaching ends, wherein the immobilized DNA fragments are complementary to one of the first DNA oligo and the second DNA oligo.

The invention relates to the technical field of tumor immunotherapy. The invention provides a response type antigen capture nano platform as well as a preparation method and application thereof. The product disclosed by the invention is applied through intravenous injection and other systemic administration modes; peroxynitrite in a tumor microenvironment can be specifically responded, and accurate activation of a tumor part is realized; tumor-associated antigens can be efficiently and covalently captured, and the antigens are delivered into antigen presenting cells, so that the anti-tumor immune response is enhanced; the tumor growth can be obviously inhibited by combining photodynamic therapy.