The invention discloses an immunomagnetic composite, a preparation method thereof, an antigen capture reagent, a kit and a system comprising same, and applications. The immunomagnetic composite includes: amino-functionalized magnetic microspheres formed by modifying magnetic microspheres with an amino polymer, and agglutinin jointed with the amino polymer. By means of the technical scheme, the agglutinin is actually connected to the magnetic microspheres through induction by the amino polymer coating the surfaces of the magnetic microspheres, wherein the surfaces of the magnetic microspheres are modified by a large quantity of amino groups by coating the magnetic microspheres with the amino polymer. The outer polymer has a dendritic network structure, so that conjugation load is fully increased, and steric hindrance is reduced. In addition, the target conjugate, agglutinin, is far away from the rigid surface of the magnetic microspheres, so that bioactivity of the target conjugate is guaranteed as most as possible.