73 results about "Antigen capture" patented technology

Methods for the diagnosis of visceral, cutaneous and canine leishmaniasis in a subject suspected of being infected with the parasitic protozoa Leishmania is disclosed. Disclosed are antibody-capture enzyme-linked immunosorbent assays (ELISAs) for the detection of antibodies to Leishmania parasite soluble antigens and antigen-capture ELISAs for the detection of Leishmania parasite soluble antigens in host samples. Also disclosed are immunodiagnostic kits for the detection of Leishmania parasite circulating antigens or IgM and IgG antibodies in a sample from subject having visceral, cutaneous or canine leishmaniasis. In these methods and kits, detection may be done photometrically or visually. The methods and kits also allow the visualization of Leishmania amastigotes or promastigotes in a sample.

The invention relates to a making method and an application of a CdS sensitized TiO2 environmental estrogen photoelectrochemical sensor. The method uses TiO2 as an antigen capture substrate material, a CdS photoelectric active material is in situ generated on the surface of a Cd2<+> functional TiP nanomaterial maker modified electrode through a direct Na2S dropping technology, and CdS is irritated by an LED lamp of visible light wavelength to be converted to a photoelectric current signal. The substrate material TiO2 can be well matched with CdS energy band, so the photocurrent conversion signal of CdS is further improved, thereby the competitive photoelectrochemical immunosensor for ultra sensitive detection of estradiol, estriol, diethylstilbestrol, bisphenol A, nonylphenol, oestrone and other environmental pollutants is made.

The invention provides an antibody detection double-antigen capture method using nanoparticles. A nanoparticle label and a label between the labeled antigens implement an indirect labeling by combining the label on an antigen and a ligand which is labeled on the nanoparticle label and can specifically recognize the label, wherein the labeled antigen is a gene engineering recombined antigen. With the indirect labeling manner, the sensitivity and the specificity of the method can be remarkably improved.

The invention discloses a vibrio parahaemolyticus flagellin monoclonal antibody and an antigen capture ELISA (enzyme-linked immunosorbent assay) kit. The vibrio parahaemolyticus flagellin monoclonal antibody is produced by secreting of a hybridoma cell strain with the preservation number of CGMCC (China General Microbiological Culture Collection) No.6061. The vibrio parahaemolyticus flagellin monoclonal antibody can be used for detecting vibrio parahaemolyticus. The invention also discloses a vibrio parahaemolyticus flagellin capture ELISA (enzyme-linked immunosorbent assay) kit.

The invention provides an immune agglomeration detection method based on a micro-fluidic chip. The immune agglomeration detection method comprises the following steps: dynamically enriching immunomagnetic beads by virtue of a micro-fluidic chip by controlling a specific region of a magnetic field in a chip micro-channel to form a magnetic bead plug; controlling a sample liquid to flow through the magnetic bead plug in cycle and capturing antigen in the sample liquid by the immunomagnetic beads; combining the antigen captured by the immunomagnetic beads with another magnetic beads to form two or more agglomerations of the magnetic beads; and counting the quantity of single magnetic beads and the agglomerated magnetic beads by detecting scattered light signals of the magnetic beads to obtain the concentration of the antigen in the sample. The invention provides the micro-fluidic chip. The invention further provides an immune agglomeration detection system based on the micro-fluidic chip. The detection system comprises the micro-fluidic chip, a micro-pump drive device, a micro-valve drive device, a magnetic bead plug control device and an optical detection module. According to the immune agglomeration detection method and system, achievement of a miniature immune detection instrument is facilitated; the detection limit is reduced; and detection of low-concentration antigen is achieved.

The invention discloses a porcine circovirus type 2 antigen capture ELISA kit. The kit internally comprises an enzyme labeled monoclonal antibody which is secreted by a hybridoma cell strain with the preservation number of CGMCC NO.10205. The invention also discloses a capture ELISA method for rapidly detecting the porcine circovirus type 2 and established by utilizing the monoclonal antibody. A porcine circovirus type 2 polyclonal antibody and a porcine circovirus type 2 cap protein monoclonal antibody respectively act as a capture antibody and a detecting antibody. The method can be used for detecting multiple gene type PCV2 viruses; the detection sensibility is 400 TICD50 / ml; the porcine circovirus type 2 has no crossed reaction with other swine viruses, and the toxic value determination method coincidence rate is 88%. The result shows that the method has the advantages of operation simplicity, good specificity, high sensitiveness, short time consumption and the like, can be used for estimating the toxic value of viruses, and can conveniently and rapidly control the quality of PCV2 inactivated vaccine semi-finished products.

The invention relates to a making method and an application of a CdS sensitized TiO2 environmental estrogen photoelectrochemical sensor. The method uses TiO2 as an antigen capture substrate material, a CdS photoelectric active material is in situ generated on the surface of a Cd2<+> functional TiP nanomaterial maker modified electrode through a direct Na2S dropping technology, and CdS is irritated by an LED lamp of visible light wavelength to be converted to a photoelectric current signal. The substrate material TiO2 can be well matched with CdS energy band, so the photocurrent conversion signal of CdS is further improved, thereby the competitive photoelectrochemical immunosensor for ultra sensitive detection of estradiol, estriol, diethylstilbestrol, bisphenol A, nonylphenol, oestrone and other environmental pollutants is made.

The invention belongs to the technical field of in-vitro detection, and particularly relates to a detection kit and a preparation method and application thereof. The detection kit comprises a magneticseparation reagent and an enzyme-labeled reagent; the magnetic separation reagent is prepared from troponin T antibody-coated gold magnetic particles or prepared from streptavidin-coated gold magnetic particles and a biotin-labeled troponin T antibody; and the enzyme-labeled reagent is prepared from an enzyme-labeled antibody polymer, wherein the enzyme-labeled antibody polymer is prepared from at least two enzyme-labeled antibodies and carbon bridges; the carbon bridges connect any two or more, adjacent or non-adjacent enzyme-labeled antibodies, and each carbon bridge has at least two connecting loci for connecting the enzyme-labeled antibodies; and each enzyme-labeled antibody is prepared from a detection antibody and a labeling enzyme coupled to the detection antibody, and the connecting loci are connected with the detection antibodies and / or the labeling enzymes. When chemiluminescence detection is conducted, a reaction signal value of a chemiluminescence method is amplified, thesensitivity of an antigen captured by the detection reagent can be enhanced, and the detection sensitivity is improved.

The present invention provides a system and method for detecting antigens captured on an antibody array. The method comprises the following steps of providing the antibody array having at least two antibodies, contacting the antibody array with a sample containing at least one antigen that may be captured by the antibodies disposed on the antibody array, and detecting the at least one antigen captured by the antibody array with a detecting agent that specifically binds to the antigen-bound antibodies on the antibody array, thereby the at least one antigen captured by the antibody array can be detected independent of the structures of the antigens. In a preferred embodiment, C1q is used as the detecting agent to detect antigen-bound antibodies.

The invention discloses a high-stability novel coronavirus spike protein, a related biological material, application of the related biological material, detection test paper and a detection kit, relates to the field of biotechnology and biomedicine. The bottleneck of the industry is broken through through internal crosslinking modification on the optimal antigen spike protein, namely S protein, onthe surface of novel coronavirus, The high-stability full-length S protein trimer is obtained through expression and purification in mammals, all antigen epitopes of the new coronavirus surface spikeprotein are reserved to the maximum extent, the stable S protein serves as an antigen to capture a new coronavirus antibody in a patient sample, and a novel coronavirus antibody instant detection kitwith high accuracy, high sensitivity and high stability is produced.

The invention relates to antibody characteristics used to design a whole blood Point of Care Thyroid Stimulating Hormone (TSH) immunoassay using an ELISA sandwich assay lacking one or more wash steps between the antigen capture, detection antibody addition and substrate introduction steps. This invention exhibits low cross reactivity with biologically similar interfering cross reacting species, such as Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) and Chorionic Gonadotropin (CG).

The invention discloses a vibrio vulnificus flagellin monoclonal antibody and an antigen capture ELISA (Enzyme Linked Immunosorbent Assay) kit. The vibrio vulnificus flagellin monoclonal antibody is generated and secreted by a hybridoma cell strain with the preservation number of CGMCC No.6755. The vibrio vulnificus flagellin monoclonal antibody disclosed by the invention can be used for detecting the vibrio vulnificus. The invention also discloses a vibrio vulnificus flagellin capture ELISA kit.

The invention discloses an H1N1 swine influenza virus-resistant hemagglutinin protein monoclonal antibody, a hybridoma cell line and an antigen-capture enzyme linked immunosorbent assay (ELISA) kit. The monoclonal antibody is secreted by a hybridoma cell of which the collection number is CCTCC C2010121, and has high titer and specificity. The developed antigen-capture ELISA kit comprises a solid-phase vector coated with the monoclonal antibody, the H1N1 swine influenza virus-resistant hemagglutinin protein monoclonal antibody labeled by a horse radish peroxidase, substrate reaction solution of an enzyme, positive and negative controls, cleaning solution and reaction stop solution. The kit has high specificity and sensitivity, is easy to operate, can be used for the clinical and laboratory detection of large-scale H1N1 swine influenza viruses and has wide application prospect.

It has been found that moieties containing a lipophilic domain, e.g., lipophilic pathogen activated molecular patterns (PAMPs), insert into the lipid bilayer on a cell membrane to facilitate antigen recognition by the innate immune response receptors. This changes the basic understanding of antigen recognition by the innate immune system. A sensor platform for the ultra-sensitive and specific detection of moieties containing such a lipophilic domain, e.g., PAMPs, that are associated with a disease, has now been developed. To date, this approach has been validated with Lipoarabinomannan (LAM) from Mycobacterium tuberculosis and lipopolysacharide (LPS), associated with gram-negative bacteria. This approach may be extendable to all lipophilic targets associated with pathogens and thus, is the basis of a very simple and specific sensing platform. In addition, novel applications for this technology in the selection of recognition ligands by mass spectroscopy have been identified.

The invention discloses a Vibrio cholerae flagellin monoclonal antibody and an antigen capture ELISA kit. The Vibrio cholerae flagellin monoclonal antibody is secreted by a hybridoma cell line with a preservation number of CGMCC No.6754. The Vibrio cholerae flagellin monoclonal antibody can be used for detection of Vibrio cholerae. The invention also discloses a Vibrio cholerae flagellin protein capture ELISA kit.

A method of antigen-capture assays that uses the detection of antigens secreted into liquid culture is provided. Also provided are antibodies to Mycobacterium avium complex-specific antigens, and diagnostic kits and systems for the detection of the presence of mycobacteria in liquid samples.

An improvement in heterogeneous immunoassays in microtiter plates to significantly reduce assay time, from as much as 50% up to 90% of what used to be typical assay times. The improvement involves the rotation of the liquid in a microtiter plate and during incubation times for antigen capture and during incubation times for sample labeling. This is accomplished through the insertion of fluted cylindrical stirrers in each well, and the use of a conventional, commercially available, microtiter vortexer.

The invention discloses an immunomagnetic composite, a preparation method thereof, an antigen capture reagent, a kit and a system comprising same, and applications. The immunomagnetic composite includes: amino-functionalized magnetic microspheres formed by modifying magnetic microspheres with an amino polymer, and agglutinin jointed with the amino polymer. By means of the technical scheme, the agglutinin is actually connected to the magnetic microspheres through induction by the amino polymer coating the surfaces of the magnetic microspheres, wherein the surfaces of the magnetic microspheres are modified by a large quantity of amino groups by coating the magnetic microspheres with the amino polymer. The outer polymer has a dendritic network structure, so that conjugation load is fully increased, and steric hindrance is reduced. In addition, the target conjugate, agglutinin, is far away from the rigid surface of the magnetic microspheres, so that bioactivity of the target conjugate is guaranteed as most as possible.

The invention discloses a human mycoplasma pneumoniae surface protein monoclonal antibody and an antigen capture ELISA kit. The human mycoplasma pneumoniae surface protein monoclonal antibody is secreted by a hybridoma cell line with a preservation number being CCTCC NO: C2017217. The human mycoplasma pneumoniae surface protein monoclonal antibody can be used for detecting human mycoplasma pneumoniae. The invention further discloses the human mycoplasma pneumoniae surface protein capture ELISA kit based on the monoclonal antibody.

The invention relates to low wash Thyroid Stimulating Hormone (TSH) immunoassays using an ELISA sandwich assay having limited or no wash step between the antigen capture, detection antibody addition and substrate introduction steps. This invention exhibits low cross reactivity with biologically similar interfering cross reacting species, such as Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) and Chorionic Gonadotropin (CG).

The invention relates to antibody characteristics used to design a whole blood Point of Care Thyroid Stimulating Hormone (TSH) immunoassay using an ELISA sandwich assay lacking one or more wash steps between the antigen capture, detection antibody addition and substrate introduction steps. This invention exhibits low cross reactivity with biologically similar interfering cross reacting species, such as Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) and Chorionic Gonadotropin (CG).

An improvement in heterogeneous immunoassays to significantly reduce assay time, from as much as 50% up to 90% of what used to be typical assay times. The improvement involves rotating the captured substrate during incubation times for antigen capture and during incubation times for sample labeling.

The invention discloses a high-sensitivity immunoassay device and a high-sensitivity immunoassay method based on an ultrathin optical fiber micro-flow laser, which belong to the technical field of sensing. The high-sensitivity immunoassay device comprises a pulse laser, an attenuation sheet, a beam splitter, a pulse energy meter, a cylindrical lens, the ultrathin optical fiber micro-flow laser, acollection lens, a collection optical fiber and a spectrum analyzer, wherein the ultra-thin optical fiber micro-flow laser is implemented by crosslinking streptavidin-cy3 molecules on the surface of asingle-mode optical fiber and capturing an antibody; the streptavidin-cy3 molecules are used as a gain medium of the ultra-thin optical fiber micro-flow laser, and the detection of the antigen concentration is realized by means of the antigen and capture antibody. The combination of the antigen and the capture antibody enables the streptavidin-cy3 molecules to be connected to the surface of the optical fiber by means of the detection antibody, and finally, the total streptavidin-cy3 molecules on the surface of the optical fiber are positively correlated with the concentration of the antigen.The surface of the optical fiber supports an echo wall mode and can provide optical feedback for laser generation. High-sensitivity immunoassay of antigen concentration can be realized by detecting laser output of the ultrathin optical fiber micro-flow laser.

The invention discloses a colloidal gold chromatography reagent strip for detecting a new crown antigen, a preparation method thereof and a kit. The colloidal gold chromatography reagent strip comprises a bottom plate, a nitrocellulose membrane, a coupling pad, a sample pad and absorbent paper, the sample pad, the coupling pad, the nitrocellulose membrane and the absorbent paper are sequentially connected to the bottom plate in the horizontal direction, and avidin biotin amplified colloidal gold nanoflowers are coupled to the coupling pad. The nitrocellulose membrane is modified by nanocellulose, a detection line coated with a new crown antigen capture antibody and a quality control line coated with a quality control molecule capture antibody are arranged on the nitrocellulose membrane, and the detection line and the quality control line are sequentially distributed in the chromatography direction. The colloidal gold chromatography reagent strip can be used for conveniently and quickly detecting new crown antigens of nasopharynx swabs and throat swabs with high sensitivity.