Colloidal gold chromatography reagent strip, preparation method and novel coronavirus antigen detection kit

A technology of colloidal gold and reagent strips, applied in the field of novel coronavirus antigen detection kits, can solve the problem of insufficient antibody binding ability and missed detection of viral antigens, and achieve the effects of enhancing the visual effect of the naked eye, the color rendering effect, and the sensitivity.

Active Publication Date: 2022-07-08
SHENZHEN YHLO BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Based on this, it is necessary to provide a colloidal gold chromatography reagent for detecting the new crown antigen in view of the problems that the traditional chromatography test paper needs to be equipped with professional instruments to read the fluorescence intensity and convert it into a signal value, and the antibody binding ability is not outstanding, causing the virus antigen to be missed. strip

Method used

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  • Colloidal gold chromatography reagent strip, preparation method and novel coronavirus antigen detection kit
  • Colloidal gold chromatography reagent strip, preparation method and novel coronavirus antigen detection kit
  • Colloidal gold chromatography reagent strip, preparation method and novel coronavirus antigen detection kit

Examples

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Effect test

preparation example Construction

[0052] A preparation method of a colloidal gold chromatography reagent strip 10 for detecting a novel coronavirus antigen, comprising the following steps:

[0053] a) Preparation of colloidal gold particle solution: HAuCl 4 Heat to 120°C while stirring in an intelligent magnetic stirrer, add 1 / 20-1 / 4 volume of sodium citrate (so that the amount of sodium citrate added is 1wt%), and continue to heat for 10-30min until the color of the solution is stable It is ruby-colored, and it can be considered that stable colloidal gold particles have been produced. After cooling to room temperature, it can be stored at 2°C to 8°C.

[0054] b) Preparation of colloidal gold nanoflower solution: add the colloidal gold particle solution prepared in step a) into 60-100 times the volume of purified water, heat it to 60-80°C, and then add HAuCl 4 (makes HAuCl 4 The added amount is 1 wt %), sodium citrate (so that the added amount of sodium citrate is 1 wt %) and hydroquinone solution, continuou...

Embodiment 1

[0065] This embodiment provides a colloidal gold chromatography reagent strip 10 for detecting novel coronavirus antigen, and the preparation method thereof includes the following steps:

[0066] a) Preparation of colloidal gold particle solution: HAuCl 4 Heat to 120°C while stirring in an intelligent magnetic stirrer, add 1 / 10 volume of sodium citrate (1wt%), and continue to heat for 20min until the color of the solution is ruby-colored, and stable colloidal gold particles can be considered to have been produced. , cooled to room temperature and stored at 4°C.

[0067] b) Preparation of colloidal gold nanoflower solution: add the colloidal gold particle solution prepared in step a) to 80 times the volume of purified water, heat it to 60°C, and then add HAuCl 4 (1wt%), sodium citrate (1wt%) and hydroquinone solution, stirred continuously at room temperature, centrifuged at 10000rpm for 20min, removed the supernatant, collected the synthesized colloidal gold nanoflower solutio...

Embodiment 2

[0076] In this example, the colloidal gold chromatography reagent strip 10 for detecting novel coronavirus antigens prepared in Example 1 is used to rapidly detect novel coronavirus antigens in fresh samples and match the PCR results to evaluate the colloidal gold chromatography reagent strips 10 for detecting novel coronavirus antigens. sensitivity and specificity.

[0077] By testing 514 fresh nasopharyngeal swabs at 3 different locations, the results were compared with PCR results. Add the nasopharyngeal swab to 450 μL of virus lysis solution, rotate and squeeze up and down 3-10 times, and after sufficient lysis, take 80 μL and add it to the sample hole 1062 of the colloidal gold chromatography reagent strip 10 for detecting the new crown antigen. After waiting for 15 minutes, observe the test results. The test results are shown in Table 1. From Table 1, it can be seen that the matching results are that the sensitivity is 96.49%, the specificity is 99.25%, and the accuracy ...

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Abstract

The invention discloses a colloidal gold chromatography reagent strip for detecting novel coronavirus antigen, a preparation method and a kit. The colloidal gold chromatography reagent strip includes a bottom plate, a nitrocellulose membrane, a coupling pad, a sample pad and an absorbent paper, and the sample pad, the coupling pad, the nitrocellulose membrane and the absorbent paper are sequentially connected in a horizontal direction On the bottom plate, the coupling pad is coupled with avidin-biotin-amplified colloidal gold nanoflowers, the nitrocellulose membrane is modified with nanocellulose, and the nitrocellulose membrane is provided with a novel coronavirus antigen capture The detection line of the antibody and the quality control line of the coated quality control molecule capture the antibody, and the detection line and the quality control line are sequentially distributed along the chromatographic direction. The above-mentioned colloidal gold chromatography reagent strip can conveniently, quickly and highly sensitively detect the novel coronavirus antigen in nasopharyngeal swabs and pharyngeal swabs.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a colloidal gold chromatography reagent strip for rapid and high-sensitivity detection of novel coronavirus antigens, a preparation method and a novel coronavirus antigen detection kit. Background technique [0002] In biological detection such as new coronavirus detection, existing detection technologies include fluorescent immunochromatographic test paper, colloidal gold chromatography test paper, etc. Among them, the fluorescent immunochromatography test paper is formed by placing fluorescent microspheres in the conjugate pad and combining with the antigen or antibody. The sphere-antigen-antibody complex is captured by the nitrocellulose membrane and the quality control line and detection limit antibody coated on the membrane to form a detection band. However, fluorescence chromatography test strips usually require supporting instruments. The fluorescence intensi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569G01N33/543G01N33/558G01N33/532
CPCG01N33/56983G01N33/54346G01N33/558G01N33/532G01N2333/165Y02A50/30
Inventor 钱纯亘黄茗张赛吴力强
Owner SHENZHEN YHLO BIOTECH
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