173 results about "Antigen testing" patented technology

The invention discloses an antibody for resisting HPV16 type E6 protein and E7 protein, a detection product and application of the antibody and the detection product, and relates to the technical field of monoclonal antibodies. The antibody comprises a heavy chain complementarity determining region in a heavy chain variable region as shown in SEQ ID NO: 1 and a light chain complementarity determining region in a light chain variable region as shown in SEQ ID NO: 2. The antibody can be specifically combined with HPV16 E6 protein and E7 protein, and a detection product for detecting the HPV16 E6 / E7 antigen can be developed based on the antibody provided by the invention. Tests show that a kit developed based on the monoclonal antibody has the advantages of high detection sensitivity, rapidness and strong tolerance on the HPV16 E6 / E7 antigen, can be used for quality control in the production process of the recombinant human HPV16 E6 / E7 antigen, and can efficiently and accurately quantify the antigen content in a vaccine and monitor the quality of the vaccine.

The invention discloses an anti-HCV (hepatitis C virus) core antigen monoclonal antibody, a hybridoma cell strain and application, and particularly relates to the hybridoma cell strain screened according to a common conserved sequence of core proteins of different genotypes of HCV, the antibody secreted by the hybridoma cell strain is applied to HCV antigen detection, different genotypes of hepatitis C viruses can be detected, and the monoclonal antibody has good inclusiveness and can be applied to detection of HCV antigens. The technical effect can be realized by only two antibodies, so that the production cost of the HCV antigen detection kit can be reduced, the production difficulty can be reduced, and the production efficiency can be improved.

The invention relates to the technical field of tumor neoantigen detection, and particularly discloses a neoantigen prediction method and system for a malignant solid tumor, and the method comprises the following steps: obtaining a tumor sample and a peripheral blood sample, and carrying out DNA and RNA sequencing; analyzing and processing to obtain DNA (Deoxyribonucleic Acid) sequencing data and RNA (Ribonucleic Acid) sequencing data; analyzing to obtain an HLA typing result and a sequence of the mutant protein; calculating to obtain related characteristic values of the new antigens; calculating a new antigen score; and intercepting the first N pieces with the highest scores to obtain a neoantigen prediction result of the malignant solid tumor. Starting from the action mechanism of the new antigens, according to key steps of the prediction process, a plurality of characteristic values are selected, a hard filtering index is not adopted, a product mode is adopted, and the score of the new antigens is calculated according to the score of the relevant characteristic values of the new antigens.

The utility model relates to the field of antigen detection kits, and discloses an infectious bovine rhinotracheitis virus antigen detection kit which comprises a box body, a roller is rotatably connected to the bottom side in the box body, a clamping groove is formed in the upper side of the roller, a clamping pin is connected to the clamping groove in a clamping mode, and a placing table is fixedly connected to the upper side of the clamping pin. A plurality of swab placing grooves, an extraction tube placing groove and a detection card placing groove are respectively formed in the upper side of the placing table, the plurality of swab placing grooves, the extraction tube placing groove and the detection card placing groove are distributed in a circumferential array, and sampling swabs are arranged on the inner sides of the plurality of swab placing grooves; according to the utility model, a plurality of groups of sampling swabs, sample extraction tubes and antigen detection cards which are distributed in a circumferential array manner are placed on the placement table, so that when sampling personnel go to cattle for sampling one by one, the sampling personnel can rotate to detection materials such as the next group of antigen detection cards and the like only by pressing down the push rod and switching the angle of the placement table, and the operation is convenient and the use is simple.

The invention discloses an antigen detection kit developed based on a human metapneumovirus F protein monoclonal antibody and application of the antigen detection kit, and belongs to the technical field of antibodies. The monoclonal antibody is a monoclonal antibody 1 and / or a monoclonal antibody 2; the amino acid sequence of a light chain variable region of the monoclonal antibody 1 is as shown in SEQ ID NO.6, and the amino acid sequence of a heavy chain variable region of the monoclonal antibody 1 is as shown in SEQ ID NO.8; the amino acid sequence of the light chain variable region of the monoclonal antibody 2 is as shown in SEQ ID NO.2, and the amino acid sequence of the heavy chain variable region of the monoclonal antibody 2 is as shown in SEQ ID NO.4. The kit containing the two monoclonal antibodies is used for detecting the metapneumovirus, has the characteristics of high sensitivity and high specificity, and can realize rapid detection and auxiliary diagnosis of HMPV infection. Therefore, the monoclonal antibody for resisting the F protein of the HMPV and the detection kit of the monoclonal antibody have wide application prospects and auxiliary diagnosis values.

The invention discloses a preparation method of a triple detection kit for influenza A, influenza B and new coronavirus antigens, and belongs to the field of respiratory virus in-vitro detection. In the antibody labeling stage, the antigen and the antibody can be more sufficiently combined in a manner of adding the linking arm, so that the antigen detection sensitivity can be improved. In the metal spraying stage, a novel coronavirus monoclonal antibody, an influenza A virus monoclonal antibody and an influenza B virus monoclonal antibody marked by latex microspheres are sequentially subjected to multi-layer metal spraying at the same position by a metal spraying instrument, the two antibodies are isolated by a trehalose solution, and metal spraying of three layers of antibodies isolated from each other is completed. And the cross reaction between different antibodies of the conjugate pad in the storage process is avoided, so that non-specific binding is avoided, and the storage stability is improved. The detection kit prepared by the method can specifically detect the novel coronavirus, the influenza A virus and the influenza B virus, and has the advantages of high sensitivity, good repeatability and the like.

The invention discloses an antibody for resisting HPV16 type E6 protein and E7 protein or an antigen binding fragment of the antibody, an HPV16 detection product and application of the HPV16 detection product, and relates to the technical field of medical biology. The antibody provided by the invention has the advantages of high detection sensitivity, rapidness and strong tolerance on the HPV16 E6 / E7 antigen, can be used for quality control in the production process of the recombinant human HPV16 E6 / E7 antigen, and can efficiently and accurately quantify the antigen content in a vaccine and monitor the quality of the vaccine. And the kit can also be directly used for detecting HPV16 type E6 protein and / or E7 protein.

The invention belongs to the technical field of biology, and particularly relates to a hybridoma cell strain capable of secreting a clostridium perfringens ETX monoclonal antibody with neutralizing activity and application of the hybridoma cell strain. On the basis of natural toxin, an indirect ELISA (enzyme-linked immunosorbent assay) detection method is established by utilizing a non-toxic ETX single amino acid site protein mutant, and a series of hybridoma cell strains secreting an anti-clostridium perfringens ETX monoclonal antibody are screened; the hybridoma cell strain can stably and efficiently secrete a monoclonal antibody with neutralizing activity and a monoclonal antibody without neutralizing resistance respectively, and large-scale batch production can be realized. According to the present invention, the clostridium perfringens ETX monoclonal antibody is firstly utilized to establish the ETX antigen ELISA detection method, and the method has characteristics of simple sample operation, low cost, rapid reaction, strong specificity and the like, can provide the reference for the diagnosis of clostridium perfringens, and provides the basis for the research of the related vaccine potency test substitution method.

The present invention provides an electrochemical aptamer sensor for detecting Mycobacterium tuberculosis ESAT‑6 antigen. The sandwich electrochemical biosensor uses a new type of MXene@Hemin‑AuNPs nanozyme as a signal amplifier and N‑CNTs‑AuNPs as a sensing interface to detect the Mycobacterium tuberculosis-specific antigen ESAT‑6 in the serum of tuberculosis patients, providing a new method for the diagnosis of tuberculosis.

The present invention relates to the field of antigen detection technology, and in particular to a nanobody for detecting Staphylococcus aureus enterotoxin B and its application. The nanobody comprises an amino acid sequence as shown in SEQ ID NO.1 or SEQ ID NO.2. The applications include: purification, detection, and removal of Staphylococcus aureus enterotoxin B. The present invention screened and obtained two nanobodies for SEB, which have high specificity and high affinity for SEB. Detection of SEB based on these two nanobodies has high specificity and sensitivity, which is of great value in the field of Staphylococcus aureus enterotoxin detection.

The invention relates to the technical field of antigen detection, in particular to a nano antibody for detecting staphylococcus aureus enterotoxin B and application of the nano antibody. The nano antibody comprises an amino acid sequence as shown in SEQ ID NO. 1 or SEQ ID NO. 2. The application comprises purification, detection and removal of the staphylococcus aureus enterotoxin B. The two SEB nanometer antibodies obtained through screening have high specificity and high affinity with the SEB, SEB detection based on the two nanometer antibodies has high specificity and sensitivity, and the SEB nanometer antibodies have important value in the field of staphylococcus aureus enterotoxin detection.

The present disclosure discloses a detection kit and a preparation method thereof and a detection method for novel coronavirus, and relates to the technical field of biomedicine, including an antigen test strip. The antigen test strip includes a substrate, bibulous paper, immune nitrocellulose membrane, and immune microsphere pad. The immune microsphere pad, the immune nitrocellulose membrane and the bibulous paper are pasted on the substrate. The immune microsphere pad is coated with latex microsphere-labeled novel coronavirus SARS-CoV-2 monoclonal antibody 1. The immune nitrocellulose membrane is provided with a test line coated with novel coronavirus SARS-CoV-2 monoclonal antibody 2 and a quality control line coated with goat anti-mouse IgG polyclonal antibody. The present disclosure uses latex particles as labeled tracer, and uses antigen-antibody reaction and lateral chromatography to detect and analyze targets, having advantages of convenience and swift.

This disclosure provides an immunoassay with exceptional sensitivity for detecting a target antigen in a sample. In one disclosure, the capture and detection steps of such an assay utilize recombinant antibodies. The disclosed immunoassay may find specific applications in the detection of HBV antigen.

According to the kit for detecting the helicobacter pylori CagA subtype and the application of the kit, 3E67 and 5T68 monoclonal antibodies are prepared by using CagA dominant immune epitopes, and the monoclonal antibodies are used in a homogeneous chemiluminescence system for the first time to prepare an antigen for detecting the helicobacter pylori CagA subtype. A murine antibody is prepared through immunization of dominant epitopes of helicobacter pylori CagA protein, an antibody pair with good specificity and strong binding force is obtained through an in-vitro screening pairing strategy, and amino acid sequences of a heavy chain variable region and a light chain variable region of the antibody pair are obtained through sequencing. Products for detecting the helicobacter pylori-related CagA antigen are developed on the basis of a chemiluminescence platform, an enzyme-linked immunosorbent assay platform and an immune layer system platform, and the kit has the advantages of high sensitivity, good specificity and rapid detection.

The invention provides a monoclonal antibody aiming at human metapneumovirus (hMPV) or an antigen binding fragment thereof, a related product thereof, and a preparation method and application thereof. The monoclonal antibody or the antigen binding fragment of the monoclonal antibody can be specifically bound with each subtype F protein of the hMPV with higher affinity, and has higher neutralizing activity on each subtype virus of the hMPV, so that the monoclonal antibody or the antigen binding fragment of the monoclonal antibody has extremely high potential to be developed into an hMPV antigen detection kit and an hMPV broad-spectrum therapeutic antibody; the method shows a remarkable application prospect in the field of clinical detection and treatment of the hMPV, and has an extremely high application value.

The application provides a lateral flow chromatography test paper and application thereof. The lateral flow chromatography test paper comprises a quality control line and an absorption pad. The lateral flow chromatography test paper further comprises an intercept line arranged between the quality control line and the absorption pad, and the material of the intercept line is one or a mixture of two or more of gelatin, carrageenan, polyacrylamide hydrogel, polyacrylic acid hydrogel, polyvinyl alcohol hydrogel or polyvinyl alcohol-sodium alginate hydrogel solution. The lateral flow chromatography test paper can improve the sensitivity of commercial test paper strips, and can be applied to new crown antigen detection, human chorionic gonadotropin detection (HCG) and cardiac troponin detection. The lateral flow chromatography test paper provided by the application can significantly improve the sensitivity by adding a cheap hydrogel intercept line after the quality control line, has good universality, and can be widely applied to various colloidal gold method test paper detection and immunofluorescence test paper detection.

The present invention provides a biological sample extract and its application and use method. The biological sample extract includes a surfactant and salt ions, wherein the surfactant is selected from Triton X-100 or Tween-20 and a high concentration of salt ions. The biological sample extract of the present invention not only improves the detection sensitivity of immunochromatographic antigen detection products, but also enhances the extract's ability to inactivate various viruses, including the novel coronavirus, playing an important role in preventing secondary contamination and protecting the public.

The utility model provides a bagging mechanism of an antigen detection kit, and belongs to the technical field of packaging bags. The problem that at present, when materials are contained in a packaging bag, the packaging bag needs to be manually held by hand, a bag opening is opened, and the labor load of workers is large is solved. The bagging mechanism for the antigen detection kits comprises a machine frame and external conveying mechanisms which are arranged on the machine frame and used for conveying the detection kits and drying agents, the output ends of the two external conveying mechanisms are located at the bagging operation position below the machine frame, and the bagging operation position is further connected with an installation base used for containing packaging bags. A mounting seat is arranged in the middle of the rack, a blocking needle is further arranged right in front of the mounting seat, the blocking needle and the mounting seat form a rhombic bagging opening, the blocking needle is connected to the middle of the rack through a displacement mechanism, and a supporting plate capable of guiding a detection kit and a drying agent into a packaging bag is further arranged above the bagging opening. In this way, the packaging bag is convenient to place, meanwhile, the bag opening of the packaging bag is convenient to open, and subsequent detection kits and drying agents can be conveniently conveyed into the packaging bag.

The application provides a YKL-40 monoclonal antibody and a preparation method and application thereof, and relates to the technical field of biological medicines.The monoclonal antibody 10F1 provided by the application has good titer.The heavy chain variable region and the light chain variable region of the monoclonal antibody are respectively inserted into different basic vectors, and the two kinds of recombinant vectors are mixed and then co-transfected into basic cells, so that a recombinant cell capable of expressing the monoclonal antibody 10F1 is obtained.The monoclonal antibody vector obtained by the application is easy to preserve and easy to control the quality of the antibody production process; the monoclonal antibody vector can recognize YKL-40 protein and has biological activity.The monoclonal antibody provided by the application can be used for YKL-40 antigen detection and other scientific researches, and has very good application value and very important scientific research guiding significance.

The utility model relates to an HIVP24 antigen detection test paper joint card which not only can improve the HIVP24 antigen quality inspection efficiency, but also is convenient to use and produce, and can reduce the quality inspection cost. The cavity of the lower shell is divided into six independent chambers which are distributed side by side through five partition plates, and an HIVP24 antigen detection test strip is arranged in each of the six independent chambers; the four corners of the lower shell are each provided with a first mounting column, the middles of the five partition plates are each provided with a second mounting column, the lower end face of the upper shell is provided with four first inserting columns, and the lower end face of the upper shell is provided with five second inserting columns. The second installation columns and the corresponding second insertion holes are installed on the lower shell after being in one-to-one insertion fit. The test paper card has the advantages that the test paper card not only can improve the HIVP24 antigen quality inspection efficiency, but also is convenient to use; 2, the test paper joint card is convenient to produce, and the quality inspection cost can be reduced.

This invention belongs to the field of image processing technology and discloses a method, device, equipment, and storage medium for judging the results of COVID-19 antigen detection. By calling a camera device to acquire the detection image based on the user's shooting command input on the user interface, image recognition is performed to complete the antigen detection result acquisition, thereby enabling rapid interpretation of the detection results. Simultaneously, the detection image undergoes a qualification verification process; only after passing the verification is the antigen card area further detected. An affine transformation is performed on the antigen card area to obtain a corrected scaled image before window detection. Finally, the detected window areas are classified. This avoids capturing unqualified images from affecting subsequent recognition results, reduces the probability of false detection, achieves a coarse-to-fine detection process, and improves the accuracy of interpretation.

The invention discloses a fusion protein, nano-particles formed by self-assembly of the fusion protein, and a polyantibody taking the nano-particles as a carrier, and also provides a preparation method and application of the product. The nanoparticles provided by the invention can efficiently load one or more monoclonal antibodies so as to prepare a polyantibody; the Fab antigen binding domain of the polyantibody is spatially ordered and radially arranged on the surface of the nanoparticle, the structural characteristic greatly improves the sensitivity of antigen detection, and the polyantibody has important clinical application value for rapid detection and early warning of related antigens or viruses, and has good industrialization prospects.

The invention belongs to the technical field of immunodetection, and discloses a VB12 sandwich method antibody mAb10 or an antigen binding fragment thereof, and a preparation method and application thereof. The VB12 sandwich method antibody mAb10 or the antigen binding fragment thereof provided by the invention comprises a light chain variable region and a heavy chain variable region, the light chain variable region comprises complementary determining regions LCDR1, LCDR2 and LCDR3, and the heavy chain variable region comprises complementary determining regions HCDR1, HCDR2 and HCDR3. When the VB12 sandwich method antibody mAb10 is used for detecting a VB12 standard antigen, the detection sensitivity is lower than 0.02 ng / mL, a clinical sample is detected through a magnetochemiluminescence method, the correlation with R clinical comparison is good in the sample range of 0-2 ng / mL, and the VB12 sandwich method antibody mAb10 has important significance in the field of diagnosis and treatment of VB12 deficiency related diseases.

1. Name of the product of this design: Antigen detection device. 2. Purpose of this product design: capable of performing multiple antigen tests. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: three-dimensional picture. 5. Description of other situations that need explanation: The usage state reference diagram shows four antigen test boxes that can be connected to the antigen detection device.

The invention provides an antigen of a porcine epidemic diarrhea virus, an ELISA detection reagent, a kit, application and a nucleic acid molecule. The antigen comprises a PEDV S protein mutant, and the PEDV S protein mutant has an amino acid sequence as shown in SEQ ID NO.3; the ELISA detection reagent comprises the antigen; the ELISA detection kit comprises the ELISA detection reagent, and the ELISA detection kit comprises the ELISA detection reagent. According to the invention, the mutated PEDV S protein is used as an antigen for ELISA detection, so that the mutant PEDV S protein has excellent effects in the aspects of specificity, repeatability and the like, and the sensitivity of the mutant PEDV S protein is obviously superior to that of a wild type PEDV S protein.

The invention discloses an antigen detection kit for helicobacter pylori in gastric and duodenal mucosa mucus, and relates to the field of medical treatment, after a sampling brush is used for fully brushing mucus on the surfaces of gastric and duodenal mucosa through a gastroscope, the sampling brush is directly inserted into a strip-shaped hole, then a first finger pressing part and a second finger pressing part are respectively pinched by the index finger and the middle finger, and the first finger pressing part and the second finger pressing part are pressed; when the rubbing frame slides, the sampling brush is pressed into the upper portion of the rubbing frame along with rolling, and the sampling brush is extruded to be located on the rubbing frame and rubbed repeatedly under the cooperation of the pressure applying part. And a mucus sample adhered to the rubbing frame is fully collected and uniformly distributed on the rubbing frame. According to the helicobacter pylori antigen extraction device, the sampling brush is directly inserted into the device, standardization is achieved through fixed pressing times, the requirement for the stability of the helicobacter pylori antigen extraction amount is met on the basis of labor saving and simple working procedures, and the false negative result caused by complex manual operation steps or insufficient sample separation is avoided.

The present disclosure may provide compositions and methods for detecting an antigen. Compositions of the disclosure may comprise a plurality of antigen binders configured to bind to antigens. Antigen binders may be coupled or linked to nucleic acid molecules such that a nucleic acid molecule of one antigen binder may at least partially hybridize to another nucleic acid molecule of another antigen binder. The nucleic acid sequence may include barcode sequences that may be displaced from one another by a strand-displacing polymerase. Methods of the present disclosure may comprise contacting an antigen with one or more antigen binders to form a complex comprising the antigen and the one or more antigen binders.

The present invention relates to a thymic tumor microenvironment antigen detection kit and its application in myasthenia gravis evaluation. The thymic tumor microenvironment antigen detection kit includes quantum dot-coupled antibody AchR, quantum dot-coupled antibody Titin, quantum dot-coupled antibody MuSK, quantum dot-coupled antibody LRP4 and quantum dot-coupled antibody RyR; quantum dot staining is performed on thymic tumor tissue sections by the kit, and different antigen staining can be performed on the same tissue section at the same time. There are differences in the expression levels of AchR, Titin, MuSK, LRP4 and RyR antigens in patients with simple thymoma, simple myasthenia gravis and thymoma combined with myasthenia gravis, and this difference can be used to distinguish the cause, course and prognosis of myasthenia gravis. The method of using quantum dots to directly carry specific antibodies eliminates the complex staining link of secondary antibodies and improves work efficiency; and observes its expression level and spatial position.

The invention discloses application of a reagent for detecting an SLC17A7 autoantibody in diagnosis of nervous system diseases. By comparing different fluorescence modes of autoimmune antibodies related to IgG of healthy people in mouse brain tissue slices of patients with nervous system diseases, it is found that fluorescence signals only exist in the patients with nervous system diseases and do not exist in the healthy people, and it is prompted that the patients possibly have the autoimmune antibodies. According to the invention, IgG specific protein related to nervous system diseases is sought through a co-immunoprecipitation technology, a specific SLC17A7 autoantibody is found through cell immunofluorescence (CBA) detection, verification is carried out in cerebrospinal fluid, and finally it is determined that the SLC17A7 autoantibody can be used as a specific marker for diagnosis of nervous system autoimmune diseases. The SLC17A7 is taken as a detection antigen, detection of the expression of the SLC17A7 autoantibody can be applied to a kit for detecting the SLC17A7 autoantibody, the kit can be applied to detection of nervous system diseases, markers for identifying the nervous system diseases are enriched, and the detection accuracy of nervous system related diseases is improved.

The present application relates to the application of enzyme-linked immunoassay for detecting the in vitro relative potency of HPV-VLP antigen stock solution and vaccine finished product (types 6, 11, 16 and 18). The enzyme-linked immunoassay of the present application has universality for detecting antigens produced by different expression systems (Hansenula, Pichia, Escherichia coli and insect cells), and improves the standardization level of HPV vaccine quality evaluation, and lays a foundation for promoting the research and development of high-quality HPV vaccine.