94 results about "Antigen testing" patented technology

The utility model relates to the field of antigen detection kits, and discloses an infectious bovine rhinotracheitis virus antigen detection kit which comprises a box body, a roller is rotatably connected to the bottom side in the box body, a clamping groove is formed in the upper side of the roller, a clamping pin is connected to the clamping groove in a clamping mode, and a placing table is fixedly connected to the upper side of the clamping pin. A plurality of swab placing grooves, an extraction tube placing groove and a detection card placing groove are respectively formed in the upper side of the placing table, the plurality of swab placing grooves, the extraction tube placing groove and the detection card placing groove are distributed in a circumferential array, and sampling swabs are arranged on the inner sides of the plurality of swab placing grooves; according to the utility model, a plurality of groups of sampling swabs, sample extraction tubes and antigen detection cards which are distributed in a circumferential array manner are placed on the placement table, so that when sampling personnel go to cattle for sampling one by one, the sampling personnel can rotate to detection materials such as the next group of antigen detection cards and the like only by pressing down the push rod and switching the angle of the placement table, and the operation is convenient and the use is simple.

The invention discloses an antigen detection kit developed based on a human metapneumovirus F protein monoclonal antibody and application of the antigen detection kit, and belongs to the technical field of antibodies. The monoclonal antibody is a monoclonal antibody 1 and / or a monoclonal antibody 2; the amino acid sequence of a light chain variable region of the monoclonal antibody 1 is as shown in SEQ ID NO.6, and the amino acid sequence of a heavy chain variable region of the monoclonal antibody 1 is as shown in SEQ ID NO.8; the amino acid sequence of the light chain variable region of the monoclonal antibody 2 is as shown in SEQ ID NO.2, and the amino acid sequence of the heavy chain variable region of the monoclonal antibody 2 is as shown in SEQ ID NO.4. The kit containing the two monoclonal antibodies is used for detecting the metapneumovirus, has the characteristics of high sensitivity and high specificity, and can realize rapid detection and auxiliary diagnosis of HMPV infection. Therefore, the monoclonal antibody for resisting the F protein of the HMPV and the detection kit of the monoclonal antibody have wide application prospects and auxiliary diagnosis values.

This disclosure provides an immunoassay with exceptional sensitivity for detecting a target antigen in a sample. In one disclosure, the capture and detection steps of such an assay utilize recombinant antibodies. The disclosed immunoassay may find specific applications in the detection of HBV antigen.

The invention provides a monoclonal antibody aiming at human metapneumovirus (hMPV) or an antigen binding fragment thereof, a related product thereof, and a preparation method and application thereof. The monoclonal antibody or the antigen binding fragment of the monoclonal antibody can be specifically bound with each subtype F protein of the hMPV with higher affinity, and has higher neutralizing activity on each subtype virus of the hMPV, so that the monoclonal antibody or the antigen binding fragment of the monoclonal antibody has extremely high potential to be developed into an hMPV antigen detection kit and an hMPV broad-spectrum therapeutic antibody; the method shows a remarkable application prospect in the field of clinical detection and treatment of the hMPV, and has an extremely high application value.

The application provides a lateral flow chromatography test paper and application thereof. The lateral flow chromatography test paper comprises a quality control line and an absorption pad. The lateral flow chromatography test paper further comprises an intercept line arranged between the quality control line and the absorption pad, and the material of the intercept line is one or a mixture of two or more of gelatin, carrageenan, polyacrylamide hydrogel, polyacrylic acid hydrogel, polyvinyl alcohol hydrogel or polyvinyl alcohol-sodium alginate hydrogel solution. The lateral flow chromatography test paper can improve the sensitivity of commercial test paper strips, and can be applied to new crown antigen detection, human chorionic gonadotropin detection (HCG) and cardiac troponin detection. The lateral flow chromatography test paper provided by the application can significantly improve the sensitivity by adding a cheap hydrogel intercept line after the quality control line, has good universality, and can be widely applied to various colloidal gold method test paper detection and immunofluorescence test paper detection.

The application provides a YKL-40 monoclonal antibody and a preparation method and application thereof, and relates to the technical field of biological medicines.The monoclonal antibody 10F1 provided by the application has good titer.The heavy chain variable region and the light chain variable region of the monoclonal antibody are respectively inserted into different basic vectors, and the two kinds of recombinant vectors are mixed and then co-transfected into basic cells, so that a recombinant cell capable of expressing the monoclonal antibody 10F1 is obtained.The monoclonal antibody vector obtained by the application is easy to preserve and easy to control the quality of the antibody production process; the monoclonal antibody vector can recognize YKL-40 protein and has biological activity.The monoclonal antibody provided by the application can be used for YKL-40 antigen detection and other scientific researches, and has very good application value and very important scientific research guiding significance.

This invention belongs to the field of image processing technology and discloses a method, device, equipment, and storage medium for judging the results of COVID-19 antigen detection. By calling a camera device to acquire the detection image based on the user's shooting command input on the user interface, image recognition is performed to complete the antigen detection result acquisition, thereby enabling rapid interpretation of the detection results. Simultaneously, the detection image undergoes a qualification verification process; only after passing the verification is the antigen card area further detected. An affine transformation is performed on the antigen card area to obtain a corrected scaled image before window detection. Finally, the detected window areas are classified. This avoids capturing unqualified images from affecting subsequent recognition results, reduces the probability of false detection, achieves a coarse-to-fine detection process, and improves the accuracy of interpretation.

The invention discloses a fusion protein, nano-particles formed by self-assembly of the fusion protein, and a polyantibody taking the nano-particles as a carrier, and also provides a preparation method and application of the product. The nanoparticles provided by the invention can efficiently load one or more monoclonal antibodies so as to prepare a polyantibody; the Fab antigen binding domain of the polyantibody is spatially ordered and radially arranged on the surface of the nanoparticle, the structural characteristic greatly improves the sensitivity of antigen detection, and the polyantibody has important clinical application value for rapid detection and early warning of related antigens or viruses, and has good industrialization prospects.

The invention belongs to the technical field of immunodetection, and discloses a VB12 sandwich method antibody mAb10 or an antigen binding fragment thereof, and a preparation method and application thereof. The VB12 sandwich method antibody mAb10 or the antigen binding fragment thereof provided by the invention comprises a light chain variable region and a heavy chain variable region, the light chain variable region comprises complementary determining regions LCDR1, LCDR2 and LCDR3, and the heavy chain variable region comprises complementary determining regions HCDR1, HCDR2 and HCDR3. When the VB12 sandwich method antibody mAb10 is used for detecting a VB12 standard antigen, the detection sensitivity is lower than 0.02 ng / mL, a clinical sample is detected through a magnetochemiluminescence method, the correlation with R clinical comparison is good in the sample range of 0-2 ng / mL, and the VB12 sandwich method antibody mAb10 has important significance in the field of diagnosis and treatment of VB12 deficiency related diseases.

1. Name of the product of this design: Antigen detection device. 2. Purpose of this product design: capable of performing multiple antigen tests. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: three-dimensional picture. 5. Description of other situations that need explanation: The usage state reference diagram shows four antigen test boxes that can be connected to the antigen detection device.

The present disclosure may provide compositions and methods for detecting an antigen. Compositions of the disclosure may comprise a plurality of antigen binders configured to bind to antigens. Antigen binders may be coupled or linked to nucleic acid molecules such that a nucleic acid molecule of one antigen binder may at least partially hybridize to another nucleic acid molecule of another antigen binder. The nucleic acid sequence may include barcode sequences that may be displaced from one another by a strand-displacing polymerase. Methods of the present disclosure may comprise contacting an antigen with one or more antigen binders to form a complex comprising the antigen and the one or more antigen binders.

The invention discloses application of a reagent for detecting an SLC17A7 autoantibody in diagnosis of nervous system diseases. By comparing different fluorescence modes of autoimmune antibodies related to IgG of healthy people in mouse brain tissue slices of patients with nervous system diseases, it is found that fluorescence signals only exist in the patients with nervous system diseases and do not exist in the healthy people, and it is prompted that the patients possibly have the autoimmune antibodies. According to the invention, IgG specific protein related to nervous system diseases is sought through a co-immunoprecipitation technology, a specific SLC17A7 autoantibody is found through cell immunofluorescence (CBA) detection, verification is carried out in cerebrospinal fluid, and finally it is determined that the SLC17A7 autoantibody can be used as a specific marker for diagnosis of nervous system autoimmune diseases. The SLC17A7 is taken as a detection antigen, detection of the expression of the SLC17A7 autoantibody can be applied to a kit for detecting the SLC17A7 autoantibody, the kit can be applied to detection of nervous system diseases, markers for identifying the nervous system diseases are enriched, and the detection accuracy of nervous system related diseases is improved.

The present application relates to the application of enzyme-linked immunoassay for detecting the in vitro relative potency of HPV-VLP antigen stock solution and vaccine finished product (types 6, 11, 16 and 18). The enzyme-linked immunoassay of the present application has universality for detecting antigens produced by different expression systems (Hansenula, Pichia, Escherichia coli and insect cells), and improves the standardization level of HPV vaccine quality evaluation, and lays a foundation for promoting the research and development of high-quality HPV vaccine.

To provide a compact, lightweight and portable novel antigen detection device that can perform magnetic immunity inspection.SOLUTION: An antigen detection device that detects an antigen to be detected in a container containing a sample solution including magnetic particles and the antigen to be detected which can be combined with magnetic particles comprises: a detection coil which detects a signal corresponding to a magnetic field by the sample contained in the container; an excitation coil which applies an AC magnetic field to the sample; and a support part which is arranged concentrically with the center axis of the detection coil with the container put close to the detection coil, wherein the detection coil detects a signal corresponding to a magnetic field by the sample which is applied with the AC magnetic field by the excitation coil and also in a cohesion state in which the antigen to be detected bonded to the magnetic particles coheres in the container arranged concentrically with the detection coil, and the detection coil further detects a magnetic field by the sample which is applied with the AC magnetic field by the excitation coil and also in a dispersion state in which the antigen to be detected bonded to the magnetic particles is dispersed in the container arranged concentrically with the detection coil.SELECTED DRAWING: Figure 1

Provided is a novel antigen detection method capable of detecting an antigen, as a substance to be detected, by using a signal transmission line for transmitting a high-frequency signal. This antigen detection method for detecting an antigen in a sample comprises: a step in which a sample, containing an antigen reacted with both a primary antibody for fixing the antigen to a carrier and a secondary antibody labelled with magnetic particles, is produced; a step in which the sample is placed close to or in contact with a probe in which a signal transmission line is formed; a step in which a DC magnetic field in a direction along the signal transmission line is applied to the sample by a magnetic field application means; a step in which a high-frequency signal is supplied to the signal transmission line by a signal measurement instrument electrically connected to the signal transmission line; a step in which a signal being transmitted along the signal transmission line in a state in which the DC magnetic field is being applied is measured by the signal measurement instrument over a predetermined frequency band; and a step in which the antigen is detected by an arithmetic processing means on the basis of a signal corresponding to the resonance frequency of ferromagnetic resonance observed in the predetermined frequency band.

The invention relates to the technical field of diagnostic markers, in particular to a marker combination for diagnosing active tuberculosis and distinguishing latent tuberculosis infection and active tuberculosis and application of the marker combination. The lectin combination which can be used for ATB diagnosis and can distinguish LTBI and ATB is obtained by detecting the IgG glycosylation condition in the onset process of tuberculosis, and the lectin combination can be used as a marker for ATB diagnosis and distinguishing LTBI and ATB, and has high specificity and sensitivity; a new thought and method are provided for distinguishing different stages of tuberculosis and early accurate diagnosis of ATB, and the lectin combination is combined with the tuberculosis antigen to detect the specific antibody, so that the diagnosis effect can be further improved. The marker combination and the detection product thereof provided by the invention have relatively good application potential in ATB diagnosis and LTBI and ATB distinguishing.

To provide a capture antibody that enables highly sensitive antigen detection by using single domain antibodies and a method of antigen detection using the same.SOLUTION: Provided is a capture antibody to be used in an antigen detection method using single domain antibodies. The capture antibody comprises a multimer of two or more single domain antibodies tandemly linked to each other through spacers, where each of the spacers is a peptide of 5-30 amino acid residues in length. The CV value of the end-to-end distances between the peptides as calculated by molecular dynamics simulation, that is, (standard deviation of the end-to-end distances between the peptides) / (cumulative average value of the end-to-end distances between the peptides), is 0.3 or less.SELECTED DRAWING: None

The invention belongs to the technical field of immunodetection, and discloses a folic acid sandwich method antibody mAb15 or an antigen binding fragment thereof as well as a preparation method and application of the folic acid sandwich method antibody mAb15 or the antigen binding fragment thereof. The folic acid sandwich antibody mAb15 or the antigen binding fragment thereof comprises a light chain variable region VL and a heavy chain variable region VH, the light chain variable region VL comprises complementary determining regions LCDR1, LCDR2 and LCDR3, and the heavy chain variable region VH comprises complementary determining regions HCDR1, HCDR2 and HCDR3. When the folic acid sandwich method antibody mAb15 is used for detecting a folic acid standard antigen, the detection sensitivity is lower than 0.15 ng / mL, a clinical sample is detected through a magnetochemiluminescence method, the correlation with R clinical comparison is good in the sample range of 0-20 ng / mL, and the folic acid sandwich method antibody mAb15 has important significance in the field of diagnosis and treatment of birth defects, cardiovascular diseases, tumors and neurodegenerative diseases.

The application discloses an antibody composition for detecting recombinant varicella-zoster virus glycoprotein E and application thereof, and relates to the field of medical biotechnology.The antibody comprises a monoclonal antibody 1M4, a heavy chain variable region sequence of which is shown as SEQ ID NO.1, and a light chain variable region sequence of which is shown as SEQ ID NO.2; and a monoclonal antibody 1M2, a heavy chain variable region sequence of which is shown as SEQ ID NO.3, and a light chain variable region sequence of which is shown as SEQ ID NO.4.The two monoclonal antibodies can be used for detecting the concentration of gE antigen, and can also be used for quality control and effect evaluation of a vaccine prepared by using the gE antigen.The detection method developed based on the two monoclonal antibodies has the advantages of high sensitivity, rapidness and strong tolerance for detecting the gE antigen.

The present application relates to the technical field of antibody, in particular to a recombinant dopamine rabbit monoclonal antibody and application. The present application utilizes phage display technology and alanine scanning mutagenesis technology, and screens to obtain a dopamine monoclonal antibody, compared with other antibodies and the antibody without alanine scanning mutagenesis, the affinity and specificity are strong, the detection sensitivity is high, and the consistency with the antigen detection concentration result in liquid quality is high. The antibody is used for the detection of dopamine in serum, and the test result shows that the detection sensitivity is high, the specificity is good, the repeatability is high, the stability is good, the precision is high, and the antibody has an important role for the clinical diagnosis of dopamine.

This application belongs to the field of immunoassay technology, and discloses an aldosterone sandwich antibody mAb5 or its antigen-binding fragment, its preparation method, and its applications. The aldosterone sandwich antibody mAb5 or its antigen-binding fragment includes a light chain variable region (VL) and a heavy chain variable region (VH). The light chain variable region (VL) includes complementarity-determining regions (LCDR1, LCDR2, and LCDR3), whose amino acid sequences are shown in SEQ ID NO. 1-3, respectively. The heavy chain variable region (VH) includes complementarity-determining regions (HCDR1, HCDR2, and HCDR3), whose amino acid sequences are shown in SEQ ID NO. 4-6, respectively. Using the aldosterone sandwich antibody mAb5 of this application to detect aldosterone standard antigens, the detection sensitivity is less than 7 pg / mL. Magnetochemiluminescence immunoassay of clinical samples shows good correlation with clinical results within the sample range of 0-1000 pg / mL, which is of great significance in the diagnosis and treatment of essential hypertension and other aldosterone-related diseases.

The application provides a monoclonal antibody specifically recognizing HCGP-39 and a preparation method and application thereof, and relates to the technical field of biological medicines.The application provides two monoclonal antibodies 6F6 and 6D9 of HCGP-39, and the titers are good.Based on the complementarity determining region sequences of the variable regions of the heavy chains and the light chains of the monoclonal antibodies, the variable region sequences are respectively constructed on corresponding antibody expression vectors, and the recombinant cells expressing the recombinant monoclonal antibodies can be obtained by transfecting cells.The monoclonal antibody vector obtained by the application is easy to preserve and easy to control the quality in the antibody production process; the monoclonal antibody can recognize the HCGP-39 protein, has biological activity, can be used for HCGP-39 antigen detection and the like, and has very good application value and very important scientific research guiding significance.

The application discloses a kind of based on nano antibody-monoclonal sandwich detection novel coronavirus receptor binding domain protein homogeneous method, it is related to antigen detection field.The expression vector of novel coronavirus RBD protein, nano antibody is constructed to carry out protein expression, the pairing performance of nano antibody and monoclonal antibody is identified by ELISA method, nano antibody and monoclonal antibody are coupled to the surface of fluorescent microsphere and magnetic nanometer microsphere respectively, novel coronavirus RBD protein is diluted into different concentrations using PBS buffer solution, is input into the fluorescent and magnetic nano probe of target RBD protein, fluorescence value is determined after being separated by magnetic frame, and the standard curve for detecting novel coronavirus RBD protein is established, and the minimum detection line is 0.09ng / mL.The immunofluorescence homogeneous method constructed by the application is simple in operation, fast in detection speed, less in steps, low in cost, suitable for a variety of scene detection, and lays a foundation for the development of novel coronavirus rapid detection kit.

This invention provides a high-affinity rabbit single-chain recombinant antibody, its application, and the resulting reagent kit, belonging to the field of immunology. The high-affinity rabbit single-chain recombinant antibody provided by this invention has the amino acid sequence shown in SEQ ID NO.1, named 111-scFv-Fc; and has the amino acid sequence shown in SEQ ID NO.5, named 86-scFv-Fc. The antibodies provided by this invention can recognize different antigenic determinants on the surface of avian leukosis virus (ALV). When applied to the detection of trace amounts of ALV, they exhibit advantages such as high specificity, high sensitivity, and good stability. The combination of the two single-chain recombinant antibodies shows high efficiency in ALV antigen detection, meeting the requirements for ALV detection in clinical samples, and has significant implications for clinical diagnosis and research applications.

The invention provides an anti-HPV45L1 protein monoclonal antibody as well as a preparation method and application thereof, and belongs to the technical field of biology. The monoclonal antibodies 15D4 and 17F9 provided by the invention can be specifically combined with the 45 subtype of HPV, have the characteristics of neutralizing the HPV59 pseudovirus and blocking the infection of the HPV59 pseudovirus, and can be used for detecting the HPV45 antigen. Meanwhile, the epitopes recognized by the two monoclonal antibodies are epitopes targeted by dominant neutralizing antibodies in serum after vaccine immunization, so that the epitopes can be used for antigen detection and can also be directly used for evaluating the neutralizing antibody generation capability induced by the HPV vaccine. The invention further provides a double-antibody sandwich ELISA kit, the kit can specifically recognize and quantify the HPV45LI protein with complete conformation and immunogenicity in a sample, the kit can be stably produced in batch, rapid evaluation of the in-vitro activity of the HPV vaccine is achieved, and the kit has a good application prospect.

The invention provides a hybridoma cell strain secreting an IgG1 type monoclonal antibody resisting human N-type blood group antigen, the hybridoma cell strain is named as N1C8 and preserved in the China Center for Type Culture Collection on August 7, 2025, and the preservation number is CCTCC NO: C2025231. The invention further provides a monoclonal antibody secreted by the hybridoma cell strain secreting the IgG1 type monoclonal antibody resisting the human N-type blood group antigen and application of the monoclonal antibody. The cell strain is used for preparing a monoclonal antibody for resisting the human N-type blood group antigen, salt and protein are added into the antibody to develop a human N-type blood group antigen detection reagent, and the human N-type blood group antigen detection reagent can be applied to detection of the human N-type blood group antigen, so that a human N-type blood group antigen detection method is more scientific and perfect.

The application belongs to the technical field of immune detection, and discloses an anti-T3 antibody mAb32 or an antigen binding fragment, which comprises a light chain variable region and a heavy chain variable region, the light chain variable region comprises LCDR1, LCDR2 and LCDR3, the amino acid sequence of the LCDR1 is shown as SEQ ID NO. 1, the amino acid sequence of the LCDR2 is IAS, and the amino acid sequence of the LCDR3 is shown as SEQ ID NO. 3; the heavy chain variable region comprises HCDR1, HCDR2 and HCDR3, and the amino acid sequences of the HCDR1-3 are shown as SEQ ID NO. 4-6 respectively. The anti-T3 antibody mAb32 is used for detecting T3 standard antigen, the detection sensitivity is lower than 0.2 ng / ml, the clinical sample is detected by a magnetic chemiluminescence method, and in a sample range of 1-80 pmol / L, the correlation with R clinical comparison is good.

The invention provides an anti-CD4 antibody as well as a microsphere flow immunofluorescence luminescence detection kit and application thereof, and provides two monoclonal antibodies for recognizing different binding sites of a CD4 antigen, an antibody coupling system based on magnetic fluorescent microspheres, a biotinylation detection antibody and phycoerythrin labeled streptavidin. The invention also discloses a corresponding detection method and a preparation method of the kit. The CD4 antigen detection kit and the antibody combination thereof provided by the invention can significantly improve the sensitivity, stability, specificity and flux capacity of CD4 detection, and are suitable for multiple fields of immune function evaluation, autoimmune disease evaluation, inflammatory disease research, HIV infection auxiliary diagnosis and the like.

The application provides a method for detecting estuary indicator organisms, a species-specific antigen protein is identified from natural Ampelisca abdita, a key immune dominant peptide segment is screened from the antigen protein, a monoclonal antibody with high species specificity is prepared and purified, and a high-sensitivity double-antibody sandwich ELISA detection method is established by using the antibody and a matched antibody, so that rapid and accurate detection of Ampelisca abdita in water is realized. The application establishes a high-sensitivity and high-specificity antigen detection method by screening the species-specific antigen peptide segment Tf-Pep2 of Ampelisca abdita and the corresponding monoclonal antibody mAb-Tf1, and combining the matched antibody prepared by using the full-length protein, and provides an effective technical means for rapid quantitative analysis of the species in marine ecological monitoring.

The application discloses an aptamer-nano gold test strip for screening human pulmonary tuberculosis and an application thereof, and belongs to the technical field of biological detection. The application successfully constructs an aptamer-nano gold test strip for screening human pulmonary tuberculosis, realizes rapid detection of a pulmonary tuberculosis marker ESAT-6 antigen, adopts mercapto-modified aptamer to modify nano gold to form stable gold label probes, adopts a mixed solution composed of a biotin-modified aptamer solution and a streptavidin solution, and constructs an ESAT-6 antigen test strip by using an ESAT-6 antigen solution. The constructed test strip has good sensitivity in ESAT-6 antigen detection, has good detection effect in the antigen concentration range of 100 pg / mL-500 pg / mL, and has good specificity, stability, repeatability and accuracy.