219 results about "Antigen testing" patented technology

The invention relates to the technical field of biology, and specifically provides a detection kit for a detection kit for antigens of a novel coronavirus (SARS-CoV-2). According to the detection kitprovided by the invention, the antigens of the SARS-CoV-2 are detected by virtue of a colloidal gold double antibody sandwich method, and two monoclonal antibodies and colloidal gold are mixed for labeling, so that a detection result of the antigens of the SARS-CoV-2 is accurate and high in sensitivity, and meanwhile, the detection rate can be remarkably increased. According to the detection kit,a blank in immunological detection of the SARS-CoV-2 is filled up, and field detection can be realized without a detection instrument, so that the time and the labor are saved, and operation is flexible.

A PCV2 antigen or antibody kit for detecting ELISA is composed of antigen detection plate, enzyme conjunctive matter working solution, positive control, negative control, sample diluent, 10 x concentrated cleaning solution, colour development solution A and B as well as stop buffer.

An antibody against the nucleocapsidprotein of SARS coronavirus, the method for using said antibody to detect the SARS coronavirus or its antigen, and the reagent kit and relative products for said method are disclosed.

A method for preparing EB viral protein enzyme-linked immunosorbent diagnostic kit includes selectng EBNAl (BKRF1) prote, Zta (BZLF1) protein and VCA-p18 protin in EB viral protein for diagnosing nasopharyngeal carcinoma of serodiagnosis as target antigen for detecting antibody level in blood serum, using glutathione-transferase gene fusion system to carry on clone, presentation and purification of EB viral protein for creating diagnostic kit.

The invention discloses a method for detecting a system for quickly screening a fever pathogen, and mainly relates to detection of a tuberculosis antibody, a flu antibody, a bird flu antibody, a plague antibody and an SARS antibody in blood serum. The chip mainly comprises coding microspheres, a coating antigen, a detection antibody, a biotinylated antibody and a streptavidin-phycoerythrin. The method comprises the following steps that: the coating antigen and the coding microshperes are coupled; the detection antibody is respectively coupled with the corresponding microspheres in separate specificity; the red laser excites the classified fluorescence on the spherical substrate; the type is determined according to different colors of the spherical substrates, wherein the biotinylated antibody is combined with the detection antibody; and the streptavidin-phycoerythrin is combined with the biotin of the detection antibody captured by the microshperes; the green laser excites the phycoerythrin, the number of report fluorescence molecules combined on the spherical substrate is measured, and the number is used for indirectly determining the content of the detection antibody combined onthe spherical substrate, so that whether the pathogen infection exists is determined, and the aim of quickly screening the fever pathogen is achieved.

The invention relates to an EV71 virus neutralization epitope detection kit or a reagent and a preparation method thereof. Particularly, the invention performs quantitative detection on EV71 virus antigen by adopting an HD6 monoclonal antibody with spectral activity. The kit or the reagent has the advantages of easy operation, high sensitivity and the like.

The invention relates to a tuberculosis antibody multiple antigen ELISA detection kit and a preparation method thereof, which pertains to the field of tuberculosis medical immunology diagnostic techniques and mainly uses detection antigen, enzyme-linked antihuman IgG antibodies, substrates, positive control serum of tuberculosis patients, control serum of normal person, calf serum and polystyrene microplates to form the kit, wherein, the detection antigen adopts the mycobacterium tuberculosis complex strains of lipid Arabian mannose (LAM), 38kD and 16kD to be combined with arbitrary one or more than one mycobacterium tuberculosis recombinant proteins in the recombinant proteins of MPT63, MTB48 and CFP10-ESAT6. The mycobacterium tuberculosis has high sensitivity, strong specificity and complementarity, can be used for detecting specific antitubercular antibodies in such body fluid samples as serum, hydrothorax and the like, and assisting the diagnosis and differential diagnosis of tuberculosis.

A rapid combined detection device for novel coronavirus antigens and antibodies in saliva comprises a clamping shell, a collecting bottle containing treating fluid and a sampling swab, and an antigendetection test strip and an antibody detection test strip are arranged on the clamping shell side by side; each of the antigen detection test strip and the antibody detection test strip comprises a back plate as well as a sample pad, a gold-labeled pad, an NC membrane and water absorption filter paper which are sequentially connected and arranged on the back plate, a detection line and a quality control line are arranged on the upper surface of the NC membrane, the quality control line is arranged on one side close to the water absorption paper, and the detection line is arranged on one side close to the sample pad; the rapid joint detection device for the novel coronavirus antigen and antibody in saliva is simple and convenient in sampling, easy to accept by testees, capable of effectively reducing infection risks of medical staff, safer and noninvasive in sampling, free of large equipment and instruments, simple in operation, easy in result judgment, suitable for self-detection of large-scale crowds and high in sensitivity. The antigen and antibody combined detection can shorten the window period and has high sensitivity.

The invention provides an avian leukosis double-antibody sandwich enzyme-linked immuno sorbent assay (ELISA) antigen detection kit. The kit comprises ELISA plates which are coated on anti-avian leukosis virus (ALV) p27 monoclonal antibodies, enzyme labeling anti-ALVp27 monoclonal antibodies and the like. Antibody capturing and antibody detecting are respectively conducted aiming at different antigenic determinants of p27 protein; anti-ALVp27 monoclonal antibodies coated by the ELISA plates are obtained through secretion of hybridoma cell strain ALVP27-5D3, and the enzyme labeling anti-ALVp27 monoclonal antibodies are obtained through secretion of hybridoma cell strain ALVP27-4F12. The avian leukosis double-antibody sandwich ELISA antigen detection kit is easy, convenient and fast to operate, can be used in detecting of all subgroup virus of ALV, and is suitable for all levels of veterinarian departments in the basic level and rapid and mass screening detecting of the avian leukosis of leaving and entering the country. The avian leukosis double-antibody sandwich ELISA antigen detection kit has the advantages of being low in cost, notable in economical benefit, and wide in application prospect.

The present invention comprises an immunochemical assay for determination of at least two antigens in a sample. The immunochemical assay comprises contacting a sample from a patient with a carrier molecule that contains at least two capture antibodies, each of which specifically binds to a binding moiety of an antigen in the sample. The assay further contains a detection antibody that specifically binds the same antigens on different binding moieties than binding moieties used by the capture antibodies. The detection agent is further attached to one or more detection probes to facilitate the detection of antigens in the sample. The immunochemical assay of the present invention is specifically designed to detect biochemical markers that are released at different time intervals in a patient's sample.

The invention discloses a high-affinity anti-carcinoembryonic antigen nanobody. The nanobody has three unique complementary determining regions CDR1, CDR2 and CDR3. The invention further provides an expression vector containing a nanobody variable region coding sequence and a host cell containing the expression vector, and further provides fusion protein of a nanobody variable region and alkalinephosphatase, the application of the nanobody to preparation of a carcinoembryonic antigen detection kit, a method for performing immunodetection on a carcinoembryonic antigen by applying the nanobody,and the corresponding detection kit. The anti-carcinoembryonic antigen (CEA) nanobody provided by the invention has a specific CEA recognizing and bonding ability, has the affinity which can reach 4.791E-10, and has unique antigen-determining cluster recognition sites; an excellent detection result can be obtained in CEA immunodetection, especially in a double-antibody sandwich method.

The invention provides a mycoplasma pneumonia mosaic antigen amino acid sequence containing an amino acid sequence as shown in SEQ ID NO:1 as well as a full-gene synthesized mycoplasma pneumonia mosaic antigen full-gene sequence containing an amino acid sequence as shown in SEQ ID NO:2. The invention also provides a method for constructing the two gene sequences. The invention also provides a preparation method of the mycoplasma pneumonia mosaic antigen containing full-gene synthesis, a mycoplasma pneumonia detection kit and a preparation method thereof. Mp recombinant mosaic antigen is selected as a mark material and is applied to a gold immunochromatography system, and the detection system is directly marked and captured, so the sensitivity is greatly improved, the specificity of the antigen is high, the antigen is easy in cultivation and purification, and cost is reduced; and a new method for detecting mycoplasma pneumoniae IgG rapidly and accurately is provided for clinical use, and a good market prospect is achieved.

The invention discloses a visual protein chip for detecting serum antibody of new-castle disease virus of chickens, infectious bronchitis virus of chickens, avian influenza virus and infectious bursal disease virus of chickens , which is prepared by the following steps: purifying and diluting whole proteins of the four virus respectively; pointing samples of the positive control serum, the negative control serum and the four virus proteins onto a chip carrier respectively; drying, fixing, sealing and washing the samples to obtain the visual protein chip. The visual protein chip uses the purified whole proteins as capturing antigens to detect the virus-specific antibodies in chicken serum so as to simplify the preparation technology and reduce the production cost, and the visual protein chip has better specificity but no cross, has high reliability of results and has the advantages of quickness, simplicity and convenience, high sensitivity, good specificity and the like. When the serum is diluted by 6,400 times, the visual protein chip still can detect the antibodies, the sensitivity is 400 times of that of the prior AGP detection method. According to the detection to serum samples in-place, the detection rate of the visual protein chip is higher than the proir AGP method remarkably.

The invention discloses an indirect ELISA (Enzyme Linked Immunosorbent Assay) method for detecting anti-roundworm antibody by a recombined roundworm ALAg protein antigen. In the method, RNA (Ribonucleic Acid) of a roundworm adult body or infective egg serves as a template; an ALAg gene is amplified by RT-PCR (Reverse Transcriptase-Polymerase chain Reaction); an ALAg expression carrier is built, and expression bacteria are transformed for inducible expression; recombined protein is purified; and the indirect ELISA method is built by taking the purified protein as a coated antigen. A stable and specific indirect ELISA method for an anti-roundworm body IgG is built, can be used for the clinical monitoring and diagnosis for roundworm infection and is suitable to basically and clinically widely popularize.

HIV-1 p24 antigen acridine ester chemiluminescence immunity analyzing testing method pertains to the clinical blood testing analysis method technique field. The prior HIV-1 p24 antigen testing method has problems of low sensitivity, rigmarole operations and the like. The invention uses acridine ester series compounds to mark the HIV-1 p24 antibody, adopts a double antibody/antigen sandwich method to execute immunity combination between the p24 antigen-antibody; uses excitant hydrogen peroxide, nitric acid, Triton X-100, and sodium hydrate to excite acridine ester series compounds to execute chemiluminescence reaction; and tests the number of photon to execute a qualitative and/or quantitative analysis. The invention has good correlativity with the general ELISA testing method, which has a correlation coefficient of 0.951 at the instance of P no more than 0.01; has high sensitivity, wherein, the testing limit is 0.5pg/ml; has wide detection range in 5-6 magnitude order; and the invention also has advantages of short reaction time, easier operation and the like.

The invention discloses an HPV16E7 monoclonal antibody and a preparation method and application thereof.A correct sequence of an HPV16E7 gene is obtained from a clinical specimen through cloning, the gene effectively expresses HPV16E7 fusion protein in the form of soluble protein in BL21(DE3) escherichia coli under the induction of IPTG after being correctly inserted in a pET28a(+) prokaryotic expression plasmid, the HPV16E7 monoclonal antibody is successfully prepared through a protein immunized BALB/C mouse, the antibody can recognize a prokaryotic and eukaryotic expression HPV16E7 antigen, quite high application value is achieved for detection of the HPV16E7 antigen, and a basis is provided for research of protein and related diseases and establishment of a detection method of the HPV16 antigen.

The invention relates to a test strip for detecting a novel coronavirus (2019-nCoV) antigen, the test strip comprises a colloidal gold double conjugate pad, the colloidal gold double conjugate pad comprises a streptavidin conjugate pad and a double-labeled antibody conjugate pad, and the double-labeled antibody conjugate pad is a colloidal gold and biotin label; a biotin-avidin amplification system is introduced when the colloidal gold double conjugate pad is prepared, and a double-labeled antibody is adopted, so that the antigen detection sensitivity is improved, and missing detection is effectively reduced.