135 results about "Antigen testing" patented technology

The utility model relates to the field of antigen detection kits, and discloses an infectious bovine rhinotracheitis virus antigen detection kit which comprises a box body, a roller is rotatably connected to the bottom side in the box body, a clamping groove is formed in the upper side of the roller, a clamping pin is connected to the clamping groove in a clamping mode, and a placing table is fixedly connected to the upper side of the clamping pin. A plurality of swab placing grooves, an extraction tube placing groove and a detection card placing groove are respectively formed in the upper side of the placing table, the plurality of swab placing grooves, the extraction tube placing groove and the detection card placing groove are distributed in a circumferential array, and sampling swabs are arranged on the inner sides of the plurality of swab placing grooves; according to the utility model, a plurality of groups of sampling swabs, sample extraction tubes and antigen detection cards which are distributed in a circumferential array manner are placed on the placement table, so that when sampling personnel go to cattle for sampling one by one, the sampling personnel can rotate to detection materials such as the next group of antigen detection cards and the like only by pressing down the push rod and switching the angle of the placement table, and the operation is convenient and the use is simple.

The invention discloses an antigen detection kit developed based on a human metapneumovirus F protein monoclonal antibody and application of the antigen detection kit, and belongs to the technical field of antibodies. The monoclonal antibody is a monoclonal antibody 1 and / or a monoclonal antibody 2; the amino acid sequence of a light chain variable region of the monoclonal antibody 1 is as shown in SEQ ID NO.6, and the amino acid sequence of a heavy chain variable region of the monoclonal antibody 1 is as shown in SEQ ID NO.8; the amino acid sequence of the light chain variable region of the monoclonal antibody 2 is as shown in SEQ ID NO.2, and the amino acid sequence of the heavy chain variable region of the monoclonal antibody 2 is as shown in SEQ ID NO.4. The kit containing the two monoclonal antibodies is used for detecting the metapneumovirus, has the characteristics of high sensitivity and high specificity, and can realize rapid detection and auxiliary diagnosis of HMPV infection. Therefore, the monoclonal antibody for resisting the F protein of the HMPV and the detection kit of the monoclonal antibody have wide application prospects and auxiliary diagnosis values.

The invention belongs to the technical field of biology, and particularly relates to a hybridoma cell strain capable of secreting a clostridium perfringens ETX monoclonal antibody with neutralizing activity and application of the hybridoma cell strain. On the basis of natural toxin, an indirect ELISA (enzyme-linked immunosorbent assay) detection method is established by utilizing a non-toxic ETX single amino acid site protein mutant, and a series of hybridoma cell strains secreting an anti-clostridium perfringens ETX monoclonal antibody are screened; the hybridoma cell strain can stably and efficiently secrete a monoclonal antibody with neutralizing activity and a monoclonal antibody without neutralizing resistance respectively, and large-scale batch production can be realized. According to the present invention, the clostridium perfringens ETX monoclonal antibody is firstly utilized to establish the ETX antigen ELISA detection method, and the method has characteristics of simple sample operation, low cost, rapid reaction, strong specificity and the like, can provide the reference for the diagnosis of clostridium perfringens, and provides the basis for the research of the related vaccine potency test substitution method.

The present invention relates to the field of antigen detection technology, and in particular to a nanobody for detecting Staphylococcus aureus enterotoxin B and its application. The nanobody comprises an amino acid sequence as shown in SEQ ID NO.1 or SEQ ID NO.2. The applications include: purification, detection, and removal of Staphylococcus aureus enterotoxin B. The present invention screened and obtained two nanobodies for SEB, which have high specificity and high affinity for SEB. Detection of SEB based on these two nanobodies has high specificity and sensitivity, which is of great value in the field of Staphylococcus aureus enterotoxin detection.

The present disclosure discloses a detection kit and a preparation method thereof and a detection method for novel coronavirus, and relates to the technical field of biomedicine, including an antigen test strip. The antigen test strip includes a substrate, bibulous paper, immune nitrocellulose membrane, and immune microsphere pad. The immune microsphere pad, the immune nitrocellulose membrane and the bibulous paper are pasted on the substrate. The immune microsphere pad is coated with latex microsphere-labeled novel coronavirus SARS-CoV-2 monoclonal antibody 1. The immune nitrocellulose membrane is provided with a test line coated with novel coronavirus SARS-CoV-2 monoclonal antibody 2 and a quality control line coated with goat anti-mouse IgG polyclonal antibody. The present disclosure uses latex particles as labeled tracer, and uses antigen-antibody reaction and lateral chromatography to detect and analyze targets, having advantages of convenience and swift.

This disclosure provides an immunoassay with exceptional sensitivity for detecting a target antigen in a sample. In one disclosure, the capture and detection steps of such an assay utilize recombinant antibodies. The disclosed immunoassay may find specific applications in the detection of HBV antigen.

According to the kit for detecting the helicobacter pylori CagA subtype and the application of the kit, 3E67 and 5T68 monoclonal antibodies are prepared by using CagA dominant immune epitopes, and the monoclonal antibodies are used in a homogeneous chemiluminescence system for the first time to prepare an antigen for detecting the helicobacter pylori CagA subtype. A murine antibody is prepared through immunization of dominant epitopes of helicobacter pylori CagA protein, an antibody pair with good specificity and strong binding force is obtained through an in-vitro screening pairing strategy, and amino acid sequences of a heavy chain variable region and a light chain variable region of the antibody pair are obtained through sequencing. Products for detecting the helicobacter pylori-related CagA antigen are developed on the basis of a chemiluminescence platform, an enzyme-linked immunosorbent assay platform and an immune layer system platform, and the kit has the advantages of high sensitivity, good specificity and rapid detection.

The invention provides a monoclonal antibody aiming at human metapneumovirus (hMPV) or an antigen binding fragment thereof, a related product thereof, and a preparation method and application thereof. The monoclonal antibody or the antigen binding fragment of the monoclonal antibody can be specifically bound with each subtype F protein of the hMPV with higher affinity, and has higher neutralizing activity on each subtype virus of the hMPV, so that the monoclonal antibody or the antigen binding fragment of the monoclonal antibody has extremely high potential to be developed into an hMPV antigen detection kit and an hMPV broad-spectrum therapeutic antibody; the method shows a remarkable application prospect in the field of clinical detection and treatment of the hMPV, and has an extremely high application value.

The application provides a lateral flow chromatography test paper and application thereof. The lateral flow chromatography test paper comprises a quality control line and an absorption pad. The lateral flow chromatography test paper further comprises an intercept line arranged between the quality control line and the absorption pad, and the material of the intercept line is one or a mixture of two or more of gelatin, carrageenan, polyacrylamide hydrogel, polyacrylic acid hydrogel, polyvinyl alcohol hydrogel or polyvinyl alcohol-sodium alginate hydrogel solution. The lateral flow chromatography test paper can improve the sensitivity of commercial test paper strips, and can be applied to new crown antigen detection, human chorionic gonadotropin detection (HCG) and cardiac troponin detection. The lateral flow chromatography test paper provided by the application can significantly improve the sensitivity by adding a cheap hydrogel intercept line after the quality control line, has good universality, and can be widely applied to various colloidal gold method test paper detection and immunofluorescence test paper detection.

The present invention provides a biological sample extract and its application and use method. The biological sample extract includes a surfactant and salt ions, wherein the surfactant is selected from Triton X-100 or Tween-20 and a high concentration of salt ions. The biological sample extract of the present invention not only improves the detection sensitivity of immunochromatographic antigen detection products, but also enhances the extract's ability to inactivate various viruses, including the novel coronavirus, playing an important role in preventing secondary contamination and protecting the public.

The application provides a YKL-40 monoclonal antibody and a preparation method and application thereof, and relates to the technical field of biological medicines.The monoclonal antibody 10F1 provided by the application has good titer.The heavy chain variable region and the light chain variable region of the monoclonal antibody are respectively inserted into different basic vectors, and the two kinds of recombinant vectors are mixed and then co-transfected into basic cells, so that a recombinant cell capable of expressing the monoclonal antibody 10F1 is obtained.The monoclonal antibody vector obtained by the application is easy to preserve and easy to control the quality of the antibody production process; the monoclonal antibody vector can recognize YKL-40 protein and has biological activity.The monoclonal antibody provided by the application can be used for YKL-40 antigen detection and other scientific researches, and has very good application value and very important scientific research guiding significance.

The utility model relates to an HIVP24 antigen detection test paper joint card which not only can improve the HIVP24 antigen quality inspection efficiency, but also is convenient to use and produce, and can reduce the quality inspection cost. The cavity of the lower shell is divided into six independent chambers which are distributed side by side through five partition plates, and an HIVP24 antigen detection test strip is arranged in each of the six independent chambers; the four corners of the lower shell are each provided with a first mounting column, the middles of the five partition plates are each provided with a second mounting column, the lower end face of the upper shell is provided with four first inserting columns, and the lower end face of the upper shell is provided with five second inserting columns. The second installation columns and the corresponding second insertion holes are installed on the lower shell after being in one-to-one insertion fit. The test paper card has the advantages that the test paper card not only can improve the HIVP24 antigen quality inspection efficiency, but also is convenient to use; 2, the test paper joint card is convenient to produce, and the quality inspection cost can be reduced.

This invention belongs to the field of image processing technology and discloses a method, device, equipment, and storage medium for judging the results of COVID-19 antigen detection. By calling a camera device to acquire the detection image based on the user's shooting command input on the user interface, image recognition is performed to complete the antigen detection result acquisition, thereby enabling rapid interpretation of the detection results. Simultaneously, the detection image undergoes a qualification verification process; only after passing the verification is the antigen card area further detected. An affine transformation is performed on the antigen card area to obtain a corrected scaled image before window detection. Finally, the detected window areas are classified. This avoids capturing unqualified images from affecting subsequent recognition results, reduces the probability of false detection, achieves a coarse-to-fine detection process, and improves the accuracy of interpretation.

The invention discloses a fusion protein, nano-particles formed by self-assembly of the fusion protein, and a polyantibody taking the nano-particles as a carrier, and also provides a preparation method and application of the product. The nanoparticles provided by the invention can efficiently load one or more monoclonal antibodies so as to prepare a polyantibody; the Fab antigen binding domain of the polyantibody is spatially ordered and radially arranged on the surface of the nanoparticle, the structural characteristic greatly improves the sensitivity of antigen detection, and the polyantibody has important clinical application value for rapid detection and early warning of related antigens or viruses, and has good industrialization prospects.

The invention belongs to the technical field of immunodetection, and discloses a VB12 sandwich method antibody mAb10 or an antigen binding fragment thereof, and a preparation method and application thereof. The VB12 sandwich method antibody mAb10 or the antigen binding fragment thereof provided by the invention comprises a light chain variable region and a heavy chain variable region, the light chain variable region comprises complementary determining regions LCDR1, LCDR2 and LCDR3, and the heavy chain variable region comprises complementary determining regions HCDR1, HCDR2 and HCDR3. When the VB12 sandwich method antibody mAb10 is used for detecting a VB12 standard antigen, the detection sensitivity is lower than 0.02 ng / mL, a clinical sample is detected through a magnetochemiluminescence method, the correlation with R clinical comparison is good in the sample range of 0-2 ng / mL, and the VB12 sandwich method antibody mAb10 has important significance in the field of diagnosis and treatment of VB12 deficiency related diseases.

1. Name of the product of this design: Antigen detection device. 2. Purpose of this product design: capable of performing multiple antigen tests. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: three-dimensional picture. 5. Description of other situations that need explanation: The usage state reference diagram shows four antigen test boxes that can be connected to the antigen detection device.

The invention discloses an antigen detection kit for helicobacter pylori in gastric and duodenal mucosa mucus, and relates to the field of medical treatment, after a sampling brush is used for fully brushing mucus on the surfaces of gastric and duodenal mucosa through a gastroscope, the sampling brush is directly inserted into a strip-shaped hole, then a first finger pressing part and a second finger pressing part are respectively pinched by the index finger and the middle finger, and the first finger pressing part and the second finger pressing part are pressed; when the rubbing frame slides, the sampling brush is pressed into the upper portion of the rubbing frame along with rolling, and the sampling brush is extruded to be located on the rubbing frame and rubbed repeatedly under the cooperation of the pressure applying part. And a mucus sample adhered to the rubbing frame is fully collected and uniformly distributed on the rubbing frame. According to the helicobacter pylori antigen extraction device, the sampling brush is directly inserted into the device, standardization is achieved through fixed pressing times, the requirement for the stability of the helicobacter pylori antigen extraction amount is met on the basis of labor saving and simple working procedures, and the false negative result caused by complex manual operation steps or insufficient sample separation is avoided.

The present disclosure may provide compositions and methods for detecting an antigen. Compositions of the disclosure may comprise a plurality of antigen binders configured to bind to antigens. Antigen binders may be coupled or linked to nucleic acid molecules such that a nucleic acid molecule of one antigen binder may at least partially hybridize to another nucleic acid molecule of another antigen binder. The nucleic acid sequence may include barcode sequences that may be displaced from one another by a strand-displacing polymerase. Methods of the present disclosure may comprise contacting an antigen with one or more antigen binders to form a complex comprising the antigen and the one or more antigen binders.

The invention discloses application of a reagent for detecting an SLC17A7 autoantibody in diagnosis of nervous system diseases. By comparing different fluorescence modes of autoimmune antibodies related to IgG of healthy people in mouse brain tissue slices of patients with nervous system diseases, it is found that fluorescence signals only exist in the patients with nervous system diseases and do not exist in the healthy people, and it is prompted that the patients possibly have the autoimmune antibodies. According to the invention, IgG specific protein related to nervous system diseases is sought through a co-immunoprecipitation technology, a specific SLC17A7 autoantibody is found through cell immunofluorescence (CBA) detection, verification is carried out in cerebrospinal fluid, and finally it is determined that the SLC17A7 autoantibody can be used as a specific marker for diagnosis of nervous system autoimmune diseases. The SLC17A7 is taken as a detection antigen, detection of the expression of the SLC17A7 autoantibody can be applied to a kit for detecting the SLC17A7 autoantibody, the kit can be applied to detection of nervous system diseases, markers for identifying the nervous system diseases are enriched, and the detection accuracy of nervous system related diseases is improved.

The present application relates to the application of enzyme-linked immunoassay for detecting the in vitro relative potency of HPV-VLP antigen stock solution and vaccine finished product (types 6, 11, 16 and 18). The enzyme-linked immunoassay of the present application has universality for detecting antigens produced by different expression systems (Hansenula, Pichia, Escherichia coli and insect cells), and improves the standardization level of HPV vaccine quality evaluation, and lays a foundation for promoting the research and development of high-quality HPV vaccine.

To provide a compact, lightweight and portable novel antigen detection device that can perform magnetic immunity inspection.SOLUTION: An antigen detection device that detects an antigen to be detected in a container containing a sample solution including magnetic particles and the antigen to be detected which can be combined with magnetic particles comprises: a detection coil which detects a signal corresponding to a magnetic field by the sample contained in the container; an excitation coil which applies an AC magnetic field to the sample; and a support part which is arranged concentrically with the center axis of the detection coil with the container put close to the detection coil, wherein the detection coil detects a signal corresponding to a magnetic field by the sample which is applied with the AC magnetic field by the excitation coil and also in a cohesion state in which the antigen to be detected bonded to the magnetic particles coheres in the container arranged concentrically with the detection coil, and the detection coil further detects a magnetic field by the sample which is applied with the AC magnetic field by the excitation coil and also in a dispersion state in which the antigen to be detected bonded to the magnetic particles is dispersed in the container arranged concentrically with the detection coil.SELECTED DRAWING: Figure 1

The invention provides a monoclonal antibody 3G1 capable of specifically recognizing CV-A5 virus and having neutralizing activity and application of the monoclonal antibody 3G1. The monoclonal antibody is prepared from a CV-A5 virus antigen immunized mouse and splenocytes of the mouse through a cell fusion technology, and amino acid sequences of three CDR regions of a heavy chain variable region of the monoclonal antibody sequentially comprise sequences as shown in SEQ ID NO.1-3; the amino acid sequences of the three CDR regions of the light chain variable region sequentially comprise sequences as shown in SEQ ID NO.4-6. The monoclonal antibody can be specifically combined with a CV-A5 virus, and is not combined with enteroviruses such as EV-A71, CV-A10, CV-A6, CV-A16 and the like. The monoclonal antibody targets conformational epitopes, has neutralizing activity, can specifically recognize CV-A5 viruses, is an ideal CV-A5 antigen detection antibody, and is beneficial to acceleration of the research and development process of hand-foot-mouth multivalent vaccines containing CV-A5 pathogens.

Provided is a novel antigen detection method capable of detecting an antigen, as a substance to be detected, by using a signal transmission line for transmitting a high-frequency signal. This antigen detection method for detecting an antigen in a sample comprises: a step in which a sample, containing an antigen reacted with both a primary antibody for fixing the antigen to a carrier and a secondary antibody labelled with magnetic particles, is produced; a step in which the sample is placed close to or in contact with a probe in which a signal transmission line is formed; a step in which a DC magnetic field in a direction along the signal transmission line is applied to the sample by a magnetic field application means; a step in which a high-frequency signal is supplied to the signal transmission line by a signal measurement instrument electrically connected to the signal transmission line; a step in which a signal being transmitted along the signal transmission line in a state in which the DC magnetic field is being applied is measured by the signal measurement instrument over a predetermined frequency band; and a step in which the antigen is detected by an arithmetic processing means on the basis of a signal corresponding to the resonance frequency of ferromagnetic resonance observed in the predetermined frequency band.

The invention relates to the technical field of diagnostic markers, in particular to a marker combination for diagnosing active tuberculosis and distinguishing latent tuberculosis infection and active tuberculosis and application of the marker combination. The lectin combination which can be used for ATB diagnosis and can distinguish LTBI and ATB is obtained by detecting the IgG glycosylation condition in the onset process of tuberculosis, and the lectin combination can be used as a marker for ATB diagnosis and distinguishing LTBI and ATB, and has high specificity and sensitivity; a new thought and method are provided for distinguishing different stages of tuberculosis and early accurate diagnosis of ATB, and the lectin combination is combined with the tuberculosis antigen to detect the specific antibody, so that the diagnosis effect can be further improved. The marker combination and the detection product thereof provided by the invention have relatively good application potential in ATB diagnosis and LTBI and ATB distinguishing.

To provide a capture antibody that enables highly sensitive antigen detection by using single domain antibodies and a method of antigen detection using the same.SOLUTION: Provided is a capture antibody to be used in an antigen detection method using single domain antibodies. The capture antibody comprises a multimer of two or more single domain antibodies tandemly linked to each other through spacers, where each of the spacers is a peptide of 5-30 amino acid residues in length. The CV value of the end-to-end distances between the peptides as calculated by molecular dynamics simulation, that is, (standard deviation of the end-to-end distances between the peptides) / (cumulative average value of the end-to-end distances between the peptides), is 0.3 or less.SELECTED DRAWING: None

The invention belongs to the technical field of immunodetection, and discloses a folic acid sandwich method antibody mAb15 or an antigen binding fragment thereof as well as a preparation method and application of the folic acid sandwich method antibody mAb15 or the antigen binding fragment thereof. The folic acid sandwich antibody mAb15 or the antigen binding fragment thereof comprises a light chain variable region VL and a heavy chain variable region VH, the light chain variable region VL comprises complementary determining regions LCDR1, LCDR2 and LCDR3, and the heavy chain variable region VH comprises complementary determining regions HCDR1, HCDR2 and HCDR3. When the folic acid sandwich method antibody mAb15 is used for detecting a folic acid standard antigen, the detection sensitivity is lower than 0.15 ng / mL, a clinical sample is detected through a magnetochemiluminescence method, the correlation with R clinical comparison is good in the sample range of 0-20 ng / mL, and the folic acid sandwich method antibody mAb15 has important significance in the field of diagnosis and treatment of birth defects, cardiovascular diseases, tumors and neurodegenerative diseases.

The application discloses an antibody composition for detecting recombinant varicella-zoster virus glycoprotein E and application thereof, and relates to the field of medical biotechnology.The antibody comprises a monoclonal antibody 1M4, a heavy chain variable region sequence of which is shown as SEQ ID NO.1, and a light chain variable region sequence of which is shown as SEQ ID NO.2; and a monoclonal antibody 1M2, a heavy chain variable region sequence of which is shown as SEQ ID NO.3, and a light chain variable region sequence of which is shown as SEQ ID NO.4.The two monoclonal antibodies can be used for detecting the concentration of gE antigen, and can also be used for quality control and effect evaluation of a vaccine prepared by using the gE antigen.The detection method developed based on the two monoclonal antibodies has the advantages of high sensitivity, rapidness and strong tolerance for detecting the gE antigen.

The invention relates to the technical field of antigen detection, and provides a hepatitis B virus surface antigen detection device, which comprises a detection table and a door body, the top of the detection table is provided with a first built-in cavity, and the interior of the detection table below the first built-in cavity is provided with a second built-in cavity. By arranging a fixing structure, a test tube with a to-be-detected reagent is moved between spring pieces to be pressed downwards, the test tube can be clamped under the action of the spring pieces, the test tube is prevented from displacing in a fixing cylinder, at the moment, a clamping block is moved into a movable groove, and under the elastic force action of a second reset spring, the test tube can be clamped in the movable groove. A clamping block is moved into a clamping groove, a test tube is pressed, a reset plate and a fixing cylinder are separated from each other, under the elastic force action of a first reset spring, the reset plate drives the test tube to move upwards through a spring piece, the test tube is conveniently taken out, and the function that the device is convenient to fix is achieved; therefore, the applicability of the hepatitis B virus surface antigen detection device in use is improved.

The invention relates to a perfluoroalkyl-modified DNA (deoxyribonucleic acid) nanostructure as well as a preparation method and application thereof. The method comprises the following steps: modifying a perfluoroalkyl chain on single-stranded DNA by using a solid-phase synthesis method to obtain perfluoroalkyl modified single-stranded DNA, and mixing the perfluoroalkyl modified single-stranded DNA according to a certain proportion by using a DNA dynamic self-assembly technology to prepare a perfluoroalkyl modified DNA tetrahedron, or preparing a perfluoroalkyl modified DNA tetrahedron by using a DNA origami technology to prepare a perfluoroalkyl modified DNA tetrahedron. The method comprises the following steps: designing and synthesizing a DNA nano structure with n hybrid functional arm chains, and hybridizing the DNA nano structure with perfluoroalkyl-modified single-stranded DNA to obtain the perfluoroalkyl-modified rectangular DNA origami. The method has the advantages of being simple in synthesis method, good in biocompatibility, diversified in function, high in loading capacity and the like, not only can provide excellent carriers for hydrophobic drug molecules and fluorescent small molecules, but also can be used as an ideal imaging molecular probe for < 19 > F-nuclear magnetic resonance. The method has a good application prospect in the fields of biosensing, drug delivery, antigen detection, molecular imaging, disease targeted therapy and the like.

The present application relates to the technical field of antibody, in particular to a recombinant dopamine rabbit monoclonal antibody and application. The present application utilizes phage display technology and alanine scanning mutagenesis technology, and screens to obtain a dopamine monoclonal antibody, compared with other antibodies and the antibody without alanine scanning mutagenesis, the affinity and specificity are strong, the detection sensitivity is high, and the consistency with the antigen detection concentration result in liquid quality is high. The antibody is used for the detection of dopamine in serum, and the test result shows that the detection sensitivity is high, the specificity is good, the repeatability is high, the stability is good, the precision is high, and the antibody has an important role for the clinical diagnosis of dopamine.