50 results about "Antigen testing" patented technology

The utility model relates to the field of antigen detection kits, and discloses an infectious bovine rhinotracheitis virus antigen detection kit which comprises a box body, a roller is rotatably connected to the bottom side in the box body, a clamping groove is formed in the upper side of the roller, a clamping pin is connected to the clamping groove in a clamping mode, and a placing table is fixedly connected to the upper side of the clamping pin. A plurality of swab placing grooves, an extraction tube placing groove and a detection card placing groove are respectively formed in the upper side of the placing table, the plurality of swab placing grooves, the extraction tube placing groove and the detection card placing groove are distributed in a circumferential array, and sampling swabs are arranged on the inner sides of the plurality of swab placing grooves; according to the utility model, a plurality of groups of sampling swabs, sample extraction tubes and antigen detection cards which are distributed in a circumferential array manner are placed on the placement table, so that when sampling personnel go to cattle for sampling one by one, the sampling personnel can rotate to detection materials such as the next group of antigen detection cards and the like only by pressing down the push rod and switching the angle of the placement table, and the operation is convenient and the use is simple.

This disclosure provides an immunoassay with exceptional sensitivity for detecting a target antigen in a sample. In one disclosure, the capture and detection steps of such an assay utilize recombinant antibodies. The disclosed immunoassay may find specific applications in the detection of HBV antigen.

The application provides a lateral flow chromatography test paper and application thereof. The lateral flow chromatography test paper comprises a quality control line and an absorption pad. The lateral flow chromatography test paper further comprises an intercept line arranged between the quality control line and the absorption pad, and the material of the intercept line is one or a mixture of two or more of gelatin, carrageenan, polyacrylamide hydrogel, polyacrylic acid hydrogel, polyvinyl alcohol hydrogel or polyvinyl alcohol-sodium alginate hydrogel solution. The lateral flow chromatography test paper can improve the sensitivity of commercial test paper strips, and can be applied to new crown antigen detection, human chorionic gonadotropin detection (HCG) and cardiac troponin detection. The lateral flow chromatography test paper provided by the application can significantly improve the sensitivity by adding a cheap hydrogel intercept line after the quality control line, has good universality, and can be widely applied to various colloidal gold method test paper detection and immunofluorescence test paper detection.

This invention belongs to the field of image processing technology and discloses a method, device, equipment, and storage medium for judging the results of COVID-19 antigen detection. By calling a camera device to acquire the detection image based on the user's shooting command input on the user interface, image recognition is performed to complete the antigen detection result acquisition, thereby enabling rapid interpretation of the detection results. Simultaneously, the detection image undergoes a qualification verification process; only after passing the verification is the antigen card area further detected. An affine transformation is performed on the antigen card area to obtain a corrected scaled image before window detection. Finally, the detected window areas are classified. This avoids capturing unqualified images from affecting subsequent recognition results, reduces the probability of false detection, achieves a coarse-to-fine detection process, and improves the accuracy of interpretation.

The invention discloses a fusion protein, nano-particles formed by self-assembly of the fusion protein, and a polyantibody taking the nano-particles as a carrier, and also provides a preparation method and application of the product. The nanoparticles provided by the invention can efficiently load one or more monoclonal antibodies so as to prepare a polyantibody; the Fab antigen binding domain of the polyantibody is spatially ordered and radially arranged on the surface of the nanoparticle, the structural characteristic greatly improves the sensitivity of antigen detection, and the polyantibody has important clinical application value for rapid detection and early warning of related antigens or viruses, and has good industrialization prospects.

The invention belongs to the technical field of immunodetection, and discloses a VB12 sandwich method antibody mAb10 or an antigen binding fragment thereof, and a preparation method and application thereof. The VB12 sandwich method antibody mAb10 or the antigen binding fragment thereof provided by the invention comprises a light chain variable region and a heavy chain variable region, the light chain variable region comprises complementary determining regions LCDR1, LCDR2 and LCDR3, and the heavy chain variable region comprises complementary determining regions HCDR1, HCDR2 and HCDR3. When the VB12 sandwich method antibody mAb10 is used for detecting a VB12 standard antigen, the detection sensitivity is lower than 0.02 ng / mL, a clinical sample is detected through a magnetochemiluminescence method, the correlation with R clinical comparison is good in the sample range of 0-2 ng / mL, and the VB12 sandwich method antibody mAb10 has important significance in the field of diagnosis and treatment of VB12 deficiency related diseases.

The present disclosure may provide compositions and methods for detecting an antigen. Compositions of the disclosure may comprise a plurality of antigen binders configured to bind to antigens. Antigen binders may be coupled or linked to nucleic acid molecules such that a nucleic acid molecule of one antigen binder may at least partially hybridize to another nucleic acid molecule of another antigen binder. The nucleic acid sequence may include barcode sequences that may be displaced from one another by a strand-displacing polymerase. Methods of the present disclosure may comprise contacting an antigen with one or more antigen binders to form a complex comprising the antigen and the one or more antigen binders.

To provide a compact, lightweight and portable novel antigen detection device that can perform magnetic immunity inspection.SOLUTION: An antigen detection device that detects an antigen to be detected in a container containing a sample solution including magnetic particles and the antigen to be detected which can be combined with magnetic particles comprises: a detection coil which detects a signal corresponding to a magnetic field by the sample contained in the container; an excitation coil which applies an AC magnetic field to the sample; and a support part which is arranged concentrically with the center axis of the detection coil with the container put close to the detection coil, wherein the detection coil detects a signal corresponding to a magnetic field by the sample which is applied with the AC magnetic field by the excitation coil and also in a cohesion state in which the antigen to be detected bonded to the magnetic particles coheres in the container arranged concentrically with the detection coil, and the detection coil further detects a magnetic field by the sample which is applied with the AC magnetic field by the excitation coil and also in a dispersion state in which the antigen to be detected bonded to the magnetic particles is dispersed in the container arranged concentrically with the detection coil.SELECTED DRAWING: Figure 1

The invention belongs to the technical field of immunodetection, and discloses a folic acid sandwich method antibody mAb15 or an antigen binding fragment thereof as well as a preparation method and application of the folic acid sandwich method antibody mAb15 or the antigen binding fragment thereof. The folic acid sandwich antibody mAb15 or the antigen binding fragment thereof comprises a light chain variable region VL and a heavy chain variable region VH, the light chain variable region VL comprises complementary determining regions LCDR1, LCDR2 and LCDR3, and the heavy chain variable region VH comprises complementary determining regions HCDR1, HCDR2 and HCDR3. When the folic acid sandwich method antibody mAb15 is used for detecting a folic acid standard antigen, the detection sensitivity is lower than 0.15 ng / mL, a clinical sample is detected through a magnetochemiluminescence method, the correlation with R clinical comparison is good in the sample range of 0-20 ng / mL, and the folic acid sandwich method antibody mAb15 has important significance in the field of diagnosis and treatment of birth defects, cardiovascular diseases, tumors and neurodegenerative diseases.

The present application relates to the technical field of antibody, in particular to a recombinant dopamine rabbit monoclonal antibody and application. The present application utilizes phage display technology and alanine scanning mutagenesis technology, and screens to obtain a dopamine monoclonal antibody, compared with other antibodies and the antibody without alanine scanning mutagenesis, the affinity and specificity are strong, the detection sensitivity is high, and the consistency with the antigen detection concentration result in liquid quality is high. The antibody is used for the detection of dopamine in serum, and the test result shows that the detection sensitivity is high, the specificity is good, the repeatability is high, the stability is good, the precision is high, and the antibody has an important role for the clinical diagnosis of dopamine.

This application belongs to the field of immunoassay technology, and discloses an aldosterone sandwich antibody mAb5 or its antigen-binding fragment, its preparation method, and its applications. The aldosterone sandwich antibody mAb5 or its antigen-binding fragment includes a light chain variable region (VL) and a heavy chain variable region (VH). The light chain variable region (VL) includes complementarity-determining regions (LCDR1, LCDR2, and LCDR3), whose amino acid sequences are shown in SEQ ID NO. 1-3, respectively. The heavy chain variable region (VH) includes complementarity-determining regions (HCDR1, HCDR2, and HCDR3), whose amino acid sequences are shown in SEQ ID NO. 4-6, respectively. Using the aldosterone sandwich antibody mAb5 of this application to detect aldosterone standard antigens, the detection sensitivity is less than 7 pg / mL. Magnetochemiluminescence immunoassay of clinical samples shows good correlation with clinical results within the sample range of 0-1000 pg / mL, which is of great significance in the diagnosis and treatment of essential hypertension and other aldosterone-related diseases.

The application discloses a kind of based on nano antibody-monoclonal sandwich detection novel coronavirus receptor binding domain protein homogeneous method, it is related to antigen detection field.The expression vector of novel coronavirus RBD protein, nano antibody is constructed to carry out protein expression, the pairing performance of nano antibody and monoclonal antibody is identified by ELISA method, nano antibody and monoclonal antibody are coupled to the surface of fluorescent microsphere and magnetic nanometer microsphere respectively, novel coronavirus RBD protein is diluted into different concentrations using PBS buffer solution, is input into the fluorescent and magnetic nano probe of target RBD protein, fluorescence value is determined after being separated by magnetic frame, and the standard curve for detecting novel coronavirus RBD protein is established, and the minimum detection line is 0.09ng / mL.The immunofluorescence homogeneous method constructed by the application is simple in operation, fast in detection speed, less in steps, low in cost, suitable for a variety of scene detection, and lays a foundation for the development of novel coronavirus rapid detection kit.

The invention provides an anti-HPV45L1 protein monoclonal antibody as well as a preparation method and application thereof, and belongs to the technical field of biology. The monoclonal antibodies 15D4 and 17F9 provided by the invention can be specifically combined with the 45 subtype of HPV, have the characteristics of neutralizing the HPV59 pseudovirus and blocking the infection of the HPV59 pseudovirus, and can be used for detecting the HPV45 antigen. Meanwhile, the epitopes recognized by the two monoclonal antibodies are epitopes targeted by dominant neutralizing antibodies in serum after vaccine immunization, so that the epitopes can be used for antigen detection and can also be directly used for evaluating the neutralizing antibody generation capability induced by the HPV vaccine. The invention further provides a double-antibody sandwich ELISA kit, the kit can specifically recognize and quantify the HPV45LI protein with complete conformation and immunogenicity in a sample, the kit can be stably produced in batch, rapid evaluation of the in-vitro activity of the HPV vaccine is achieved, and the kit has a good application prospect.

The invention provides a hybridoma cell strain secreting an IgG1 type monoclonal antibody resisting human N-type blood group antigen, the hybridoma cell strain is named as N1C8 and preserved in the China Center for Type Culture Collection on August 7, 2025, and the preservation number is CCTCC NO: C2025231. The invention further provides a monoclonal antibody secreted by the hybridoma cell strain secreting the IgG1 type monoclonal antibody resisting the human N-type blood group antigen and application of the monoclonal antibody. The cell strain is used for preparing a monoclonal antibody for resisting the human N-type blood group antigen, salt and protein are added into the antibody to develop a human N-type blood group antigen detection reagent, and the human N-type blood group antigen detection reagent can be applied to detection of the human N-type blood group antigen, so that a human N-type blood group antigen detection method is more scientific and perfect.

The invention provides an anti-CD4 antibody as well as a microsphere flow immunofluorescence luminescence detection kit and application thereof, and provides two monoclonal antibodies for recognizing different binding sites of a CD4 antigen, an antibody coupling system based on magnetic fluorescent microspheres, a biotinylation detection antibody and phycoerythrin labeled streptavidin. The invention also discloses a corresponding detection method and a preparation method of the kit. The CD4 antigen detection kit and the antibody combination thereof provided by the invention can significantly improve the sensitivity, stability, specificity and flux capacity of CD4 detection, and are suitable for multiple fields of immune function evaluation, autoimmune disease evaluation, inflammatory disease research, HIV infection auxiliary diagnosis and the like.

The application provides a method for detecting estuary indicator organisms, a species-specific antigen protein is identified from natural Ampelisca abdita, a key immune dominant peptide segment is screened from the antigen protein, a monoclonal antibody with high species specificity is prepared and purified, and a high-sensitivity double-antibody sandwich ELISA detection method is established by using the antibody and a matched antibody, so that rapid and accurate detection of Ampelisca abdita in water is realized. The application establishes a high-sensitivity and high-specificity antigen detection method by screening the species-specific antigen peptide segment Tf-Pep2 of Ampelisca abdita and the corresponding monoclonal antibody mAb-Tf1, and combining the matched antibody prepared by using the full-length protein, and provides an effective technical means for rapid quantitative analysis of the species in marine ecological monitoring.

The application discloses an aptamer-nano gold test strip for screening human pulmonary tuberculosis and an application thereof, and belongs to the technical field of biological detection. The application successfully constructs an aptamer-nano gold test strip for screening human pulmonary tuberculosis, realizes rapid detection of a pulmonary tuberculosis marker ESAT-6 antigen, adopts mercapto-modified aptamer to modify nano gold to form stable gold label probes, adopts a mixed solution composed of a biotin-modified aptamer solution and a streptavidin solution, and constructs an ESAT-6 antigen test strip by using an ESAT-6 antigen solution. The constructed test strip has good sensitivity in ESAT-6 antigen detection, has good detection effect in the antigen concentration range of 100 pg / mL-500 pg / mL, and has good specificity, stability, repeatability and accuracy.

The invention belongs to the technical field of immunodetection, and discloses an E2 sandwich-method rabbit monoclonal antibody mAb9 or an antigen binding fragment thereof as well as a preparation method and application of the E2 sandwich-method rabbit monoclonal antibody mAb9 or the antigen binding fragment thereof. The E2 sandwich method rabbit monoclonal antibody mAb9 or an antigen binding fragment thereof comprises a light chain variable region VL and a heavy chain variable region VH, the light chain variable region VL comprises complementary determining regions LCDR1, LCDR2 and LCDR3, and the heavy chain variable region VH comprises complementary determining regions HCDR1, HCDR2 and HCDR3. The monoclonal antibody can be combined with an E2 compound in a high-specificity mode, is suitable for double-antibody sandwich immunodetection and is applied to an immunodetection kit prepared through double-antibody sandwich ELISA or a chemiluminescence method, a double-antibody sandwich chemiluminescence platform is used for detecting an E2 standard antigen, the detection sensitivity is lower than 10 pg / mL, a clinical sample is detected through a magnetochemiluminescence method, and the detection sensitivity is lower than 10 pg / mL. Within the sample range of 0-15000 pmol / L, the correlation with clinical comparison is good, and clinical management is provided for female health and male health.

The invention provides an anti-ADGRG1 antibody as well as a microsphere flow type immunofluorescence luminescence detection kit and application thereof, and provides two monoclonal antibodies for recognizing different binding sites of an ADGRG1 antigen, an antibody coupling system based on magnetic fluorescent microspheres, a biotinylation detection antibody and phycoerythrin labeled streptavidin. The invention also discloses a corresponding detection method and a preparation method of the kit. The ADGRG1 antigen detection kit and the antibody combination thereof provided by the invention can significantly improve the sensitivity, stability, specificity and flux capacity of ADGRG1 detection, and are suitable for multiple fields of immune function evaluation, autoimmune disease evaluation, inflammatory disease research, HIV infection auxiliary diagnosis and the like.

The invention discloses a method for detecting a membranous nephropathy target antigen, which comprises the following steps: adding a protein extracting solution into a glomerular tissue to be detected, and incubating; adding a mixed enzyme of a LysC enzyme and a Trypsin enzyme, and incubating; adding a dithiothreitol solution, and incubating; adding an iodo-acetamide solution, and incubating at room temperature in a dark place; adding a precipitating agent, performing ultrasonic treatment and centrifugation, taking supernate, loading the supernate to a C18 small column, eluting the small column, and collecting eluent; freezing the eluent, blow-drying the eluent, and redissolving the eluent with a loading solution to obtain a test solution; and carrying out liquid chromatography-tandem mass spectrometry detection on the test solution. By adopting the detection method disclosed by the invention, the target antigen of membranous nephropathy can be accurately detected when the sampling volume is obviously reduced, so that the sampling difficulty is greatly reduced, and the application of the laser microdissection and liquid chromatography-mass spectrometry combined technology in the detection of the target antigen of membranous nephropathy is greatly promoted. The method is simple in process and short in detection time.

The application provides a kit for detecting sialylated carbohydrate antigen KL-6 and a use method and application thereof, and relates to the field of in vitro detection. The kit for detecting sialylated carbohydrate antigen KL-6 provided by the application comprises magnetic beads, KL-6 antibodies, acridinium ester, a magnetic bead storage solution, an acridinium ester storage solution, a sample processing solution, a pre-priming solution and a priming solution. The sample processing solution comprises, in terms of mass fraction, 0.03-0.3% polyoxyethylene fatty acid ester, 0.3-2.0% bovine serum albumin, 0.3-2.0% raffinose, 0.3%-2.0% sodium hydroxide, 0.01-1.5% NaN3 and 0.3-1.5% sodium chloride, the rest is purified water, and the pH is 8-10. The kit can be used for determining sialylated carbohydrate antigen KL-6 in serum, and has high accuracy and strong anti-interference ability.

The present invention relates to a multiplex and simultaneous antigen detection kit (200) for the rapid diagnosis of Dengue, Chikungunya, and two forms of Malaria (Plasmodium vivax and Plasmodium falciparum) from whole blood samples. Utilizing vertical flow technology, the kit allows the detection of multiple antigens concurrently, offering faster results with enhanced sensitivity and specificity compared to traditional lateral flow assays. The diagnostic device employs metal nanoparticles and gold conjugates to detect the specific antigens, facilitating co-infection detection without the need for serum or plasma separation.

The invention discloses a Klebsiella pneumoniae antigen epitope chimeric protein as well as preparation and application thereof, and relates to the fields of genetic engineering technologies, vaccines and diagnostic reagents. Amino acid sequences of Klebsiella pneumoniae outer membrane proteins OMPA, OMPN and CusC are analyzed through a computer, two B cell epitopes, one HTL epitope and one CTL epitope are respectively screened from the three outer membrane proteins, a cholera toxin subunit B (CTB) is used as an internal adjuvant, an EAAAK joint is used for connecting the amino acid sequence of the CTB and the B cell epitope of the OMPA, the B cell epitope and the HTL epitope are connected through a GPGPG flexible joint, and the CTL epitope and the CusC epitope are connected through a GMPG flexible joint. CTL inner epitopes are connected through an AAY connector to form a chimeric protein with multiple antigen fragments connected in series. A codon preferred by a pronucleus is selected, and a full-length gene of the chimeric protein is chemically synthesized. The chimeric protein is expressed and purified by using a gene engineering technology, and the chimeric protein has 312 amino acids in total length. The expressed chimeric protein can be used for developing vaccines, antibodies or antigen detection reagents.

This invention belongs to the field of biotechnology, specifically relating to a hybridoma cell line secreting monoclonal antibodies against Clostridium perfringens ETX and its applications. Based on natural toxins, this invention establishes an indirect ELISA detection method using a non-toxic ETX single-amino acid site protein mutant. A series of hybridoma cell lines secreting monoclonal antibodies against Clostridium perfringens ETX were screened. These hybridoma cell lines can stably and efficiently secrete both neutralizing monoclonal antibodies and non-neutralizing monoclonal antibodies, and can be mass-produced on a large scale. This invention is the first to establish an antigen ELISA detection method for Clostridium perfringens ETX using monoclonal antibodies. This method features simple sample handling, low cost, rapid reaction, and high specificity. It can also provide a reference for the diagnosis of Clostridium perfringens infection and lay the foundation for research on alternative methods for testing the efficacy of related vaccines.

This invention relates to a specific monoclonal antibody against the N protein of porcine epidemic diarrhea virus (PEDV) and its uses. First, this invention obtains a monoclonal antibody, 6C12, that specifically reacts with the N protein of PEDV. Second, this application establishes a double-antibody sandwich ELISA method for detecting PEDV based on a rabbit polyclonal antibody and a specific monoclonal antibody, using a rabbit polyclonal antibody against the N protein of PEDV as the capture antibody and an HRP-labeled 6C12 monoclonal antibody as the detection antibody. This method can be used for monitoring PEDV antigen levels, replacing existing RT-PCR and colloidal gold test strip antigen detection methods, and has high specificity and high sensitivity. Compared to RT-PCR, this invention is simple to operate, requires no laboratory support, and shortens the detection time. Compared to colloidal gold test strip detection, this invention is low-cost and allows for large-scale antigen detection.

The invention belongs to the field of systemic lupus erythematosus detection, and particularly discloses a SmD1amp; the invention relates to a SmD3 fusion protein and application thereof in detection of systemic lupus erythematosus. The invention provides SmD1amp, SmD1amp; the SmD3 fusion protein comprises polypeptides as shown in SEQ ID NO.1-2 which are connected through a linker connecting peptide, and the SmD3 fusion protein has an amino acid sequence as shown in SEQ ID NO.7-10. Meanwhile, the invention also provides a carrier containing the SmD1amp; the invention discloses a detection kit for SmD3 fusion protein. By using the SmD1amp; the SmD3 fusion protein is used as an antigen to detect a clinical sample, the sensitivity and the specificity are obviously improved, the production cost is reduced, and a better detection or diagnosis approach is provided for clinical systemic lupus erythematosus.

The invention belongs to the technical field of antigen detection, and relates to a method for detecting a macromolecular antigen based on GOx (at) ZIF-90 (at) PDA. The method comprises the following steps: (1) adding a GOx (at) ZIF-90 (at) PDA solution modified by a polyclonal antibody and magnetic nanoparticles modified by a monoclonal antibody into a to-be-detected sample containing a to-be-detected antigen for reaction, and carrying out magnetic separation to obtain a precipitate; (2) adding a glucose solution into the precipitate for reaction, and performing magnetic separation to obtain supernate; (3) adding an HRP solution, a TYR solution and a colloidal gold solution into the supernate obtained in the step (2) for reaction; and calculating the content of the to-be-detected antigen according to the colorimetric standard curve and / or Raman standard curve established by the to-be-detected antigen standard substance. On the basis of enzyme signal amplification, a colorimetric-SERS dual-signal mode based on solution color is established, so that the detection sensitivity can be effectively improved, and trace detection of macromolecular antigens is realized.

The invention relates to the technical field of monoclonal antibody preparation and antigen detection kit preparation, in particular to a preparation method of a monkey pox virus monoclonal antibody and an antigen detection kit of the monkey pox virus monoclonal antibody. The mouse anti-human monoclonal antibody 1 and the mouse anti-human monoclonal antibody 2 are respectively used as a colloidal gold kit marker and a coating antibody, and the mouse anti-human monoclonal antibody 1 and the mouse anti-human monoclonal antibody 2 are prepared on the basis of monkey pox virus A29L protein and are prepared by a milk ball microparticle method. The kit disclosed by the invention can be used for early screening and early diagnosis of suspected people, so that monkey pox can be quickly, conveniently and accurately diagnosed.

The invention belongs to the field of autoimmune disease detection, and particularly discloses a recombinant La / SSB polypeptide and application thereof in detection and / or diagnosis of autoimmune diseases. The invention discloses a recombinant La / SSB polypeptide. The recombinant La / SSB polypeptide comprises oligopeptides as shown in SEQ ID NO.1-4, wherein the oligopeptides are connected through a linker connecting peptide. The polypeptide is adopted as an antigen to detect an anti-La / SSB antibody in serum, the detection sensitivity reaches 90.9%, the specificity reaches 91.7%, compared with the detection sensitivity (88.6%) and the specificity (66.7%) of commercially available La / SSB full-length protein, the specificity is obviously higher than that of the commercially available La / SSB full-length protein, and meanwhile, compared with the detection sensitivity (79.5%) and the specificity (87.5%) of an AA349-364 synthetic peptide fragment, the sensitivity is also obviously improved. The recombinant La / SSB polypeptide provided by the invention has the advantages that the production cost is reduced, the detection efficiency is obviously improved, and a better detection or diagnosis way is provided for clinical autoimmune diseases.