The invention provides an
aptamer for specific recognition of
jasmonic acid methyl ester and a
screening method and application thereof. Compared with the prior art, the
screening method comprises the following steps: 1) screening of the
aptamer: carrying out heating annealing treatment on an initial ssDNA
library and a biotinylated capture probe, cooling to
room temperature, incubating with
streptavidin magnetic beads, adding target
jasmonic acid methyl ester, incubating, and collecting the
aptamer; carrying out
magnetic separation and taking supernate as a template for PCR amplification; 2) carrying out PCR amplification; 3) preparing secondary ssDNA as an initial
library of the next round; and 4) carrying out circulating screening to obtain the aptamer MeJA-52 capable of specifically recognizing the
jasmonic acid methyl ester. According to the invention,
methyl jasmonate is taken as a positive screening target,
salicylic acid and indoleacetic acid are taken as negative screening targets to improve the specificity of the aptamer, the aptamer in high-specificity binding with
methyl jasmonate is obtained through 15 rounds of screening, a new recognition element is provided for quantitative detection of
methyl jasmonate in plants, and the application prospect is wide.