38 results about "Dna hairpin" patented technology

This invention provides an antibody with high affinity for single-stranded DNA, low or no affinity for double-stranded DNA, and capable of specifically binding a DNA hairpin and the hybridoma cell lines which produces these monoclonal antibodies. A chimeric mouse comprising these hybridoma cell lines and a histocompatible mouse is further provided. A method for screening for an agent which will inhibit anti-DNA antibody.DNA binding. One such agent is a benzodiazepine derivative. This invention therefore provides a method of inhibiting the binding of an anti-DNA antibody to its DNA ligand in a sample by contacting the sample with an effective amount of a benzodiazepine derivative.

The invention provides a signal amplification and signal reporting integrated oligonucleotide probe. The probe is a pair of DNA hairpin sequences designed on the basis of a target molecule and having cohesive ends, substantially is a product combining a hybridization chain reaction fuel molecule with a G-quadruplex sequence, has the characteristic of target molecule signal cascade amplification of the hybridization chain reaction in an enzyme-free isothermal manner, and enables the amplified target molecule signal to be conveniently and simply detected through the introduction of a signal reporting element G-quadruplex sequence. The invention also provides a method of an enzyme-free isothermal nucleic acid probe mediated by the above probe. The method enables the signal of an object to be detected to be amplified and detected under a normal temperature condition without any enzymes, and has the advantages of sensitivity, accuracy, simplicity, and easy implementation.

The invention relates to the technical field of nucleic acid detection, in particular to a microRNA detection method based on non-enzymatic amplification and electrochemiluminescence principles. The detection method comprises the steps of: 1) designing DNA hairpin probes, i.e. designing the hairpin H1 and hairpin H2 sequences required by a non-enzymatic amplification system according to a non-enzymatic amplification principle and a detected microRNA sequence; 2) conducting electrochemiluminescence group marking; 3) performing hairpin probe pretreatment; 4) constructing a non-enzymatic amplification detection system; 5) carrying out product cleaning and separation; and 6) conducting signal detection. The method has the characteristics of high sensitivity, accuracy, fastness and simple operation, and the whole amplification process does not involve enzyme, so that the detection cost is greatly reduced.

The invention provides a suturing Toehold activation method used for controlling a DNA strand displacement reaction and a toolkit. A novel Toehold activation method is developed based on hairpin reconstruction caused by mutual action of a DNA hairpin structure and different environmental irritants (Hg2+ or ATP). The invention develops a novel Toehold activation method. The extra level of the DNA strand displacement reaction can be accurately controlled by changing the concentration of the environmental irritants.

The invention provides a nucleic acid detection method based on a DNA (Deoxyribonucleic Acid) hairpin and RCA (Rolling Circle Amplification). The detection method comprises the steps of: modifying the DNA hairpin to a solid carrier, wherein the circular part of the DNA hairpin is complementary with a target DNA and the stem part of the DNA hairpin is complementary with an an annular DNA; adding the annular DNA, an enzyme, an amplification raw material and the like; complementing and pairing a target nucleic acid molecule and the loop of the DNA hairpin when the target nucleic acid molecule is added into a detection system; opening a hairpin-loop structure; hybridizing a base of the hairpin and the circular DNA; performing RCA; opening different hairpin-loop structures when the target nucleic acid molecular weights are different; and quantifying the target nucleic acid molecule by using an optical or electric method. According to the nucleic acid detection method, as the DNA hairpin is used as a detection probe of RCA, the step of detecting the molecule by RCA is simplified, the detection cost is reduced, the advantage of high sensitivity of RCA for nucleic acid detection is simultaneously kept, and the nucleic acid detection method is suitable for popularization and use.

The invention discloses a highly sensitive and decomposable quantum dot nanosphere probe and a preparation method thereof. Through a fulcrum-mediated strand displacement reaction, a DNA hairpin probe modified by three desulfated biotins is self-assembled into a Y-type DNA nanostructure; and the Y-type DNA structure and quantum dots coupled to streptavidin are self-assembled to form the quantum dot nanosphere probe. The prepared quantum dot nanosphere probe and an immunomagnetic separation technology are combined to be applied to quick detection of a target analyte. Because of a binding force between biotin and streptavidin stronger thana binding force of desulfurized biotin, the quantum dot nanosphere probe is decomposed, and thus quantum dots are released; the target analyte concentration is determined by detecting the fluorescence value of a quantum dot solution, and a shielding effect of immunomagnetic beads on the quantum dots is overcome. The method has high sensitivity and the detection limit of microorganisms is 1.37 cfu/mL. This method is good in specificity, and common other microorganisms do not affect the detection.

The invention provides a gene mutation detection method using a connection-based loop-mediated isothermal amplification technology. The detection method comprises the following steps: (1) designing two DNA hairpin probes H1 and H2 by taking target DNA as a template; (2) in the presence of target, performing annealing hybridization of the target DNA with the H1 and H2; and in the case of complete complementation of the 3' end of H1, connecting the H1 and H2 by ligase to form a double-dumbbell DNA structure; (3) performing a loop-mediated isothermal amplification reaction by taking the double-dumbbell DNA as a template to obtain a loop-mediated isothermal amplification product; (4) adding DNA double-chain specific dye SYBR GreenI into the loop-mediated isothermal amplification product to enhance the fluorescence signal, and implementing quantitative detection of gene mutation through fluorescence signal detection. The gene mutation detection method provided by the invention has remarkably low lower detection limit and relatively high selectivity and specificity.

The present invention relates to an AND gate molecular circuit, a NOT gate molecular circuit, an XOR molecular circuit and a half-subtracter molecular circuit based on a DNA hairpin structure, belonging to the biocomputer technology field. The principles of the DNA single hairpin structure, the double hairpin structure and the two-way strand displacement reaction are employed to design a strand displacement reaction structure taking a conventional small fulcrum as an identification area to realize the construction of AND gate, NOT gate and XOR gate basic logic operation structures and perform globality simulation argumentation. A basic logic circuit is employed to perform combination design of the half-subtracter molecular circuit and perform argumentation simulation of the circuit feasibility.

Theranostic nanoprobes are provided for overcoming cancer multidrug resistance, and methods for treating biological tissue, including cancerous tissue. The theranostic nanoprobes may include gold nanoparticles functionalized with DNA-hairpin. The DNA-hairpin may be configured to hybridize to a complementary target, which may silence or lessen the multidrug resistance of cancer cells. The theranostic nanoprobes may be configured to release a chemotherapeutic agent upon hybridization of the DNA-hairpin to a target molecule.

The invention relates to the technical field of DNA self-assembled membranes, particularly to a method for quantitative evaluating the HP DNA hairpin configuration on a substrate surface based on enzymic hydrolysis ability and a background signal eliminating method based on enzymic hydrolysis ability. The specific steps comprise: removing dimers through high temperature denaturation; immersing a substrate in an exonuclease I buffer solution having an enzyme amount of 100-300 U, and carrying out hydrolysis; and determining the proportion of the HP DNA hairpin configuration on the surface of anelectrode by using an electricity integration technology. According to the present invention, exonuclease has difference between in hydrolysis of ssDNA and in hydrolysis of HP DNA hairpin configuration, and the electrochemical method is combined to quantitatively evaluate the proportion of the HP DNA hairpin configuration on the surface of the substrate; and with the exonuclease I, the backgroundsignal caused by non-hairpin configuration can be effectively eliminated so as to substantially improve the detection sensitivity of the Hairpin DNA-based biosensor.

The invention belongs to the technical field of bioluminescence analysis, and particularly relates to an amphiphilic DNA nano-micelle and a preparation method and application thereof. The preparationmethod comprises the following steps: 1, designing a specific DNA hairpin sequence H1-5 of a target miRNA, modifying a hydrophobic C12 alkane chain at the tail end of H3 to prepare sp-H3 freeze-driedpowder, preparing a sp-H3 dispersion liquid with a hairpin structure from the obtained sp-H3 freeze-dried powder, finally adding a fluorescent dye into the dispersion liquid, and carrying out ultrasonic treatment to obtain the DNA nano-micelle. The invention also provides an application of the DNA micelle nanometer in label-free detection of target miRNA. The DNA micelle constructed by the invention not only can be quickly assembled into a spherical nano structure, but also has relatively large immobilization capacity, meanwhile, the amphiphilic DNA nano-micelle has the characteristics of goodselectivity, convenience in operation and the like, and has important significance for measuring small biological molecules.

The invention relates to a ratio-type electrochemical biosensor for eliminating LAMP background interference, a construction method and application. According to the method, a target object short-chain DNA is used as an LAMP amplification outer primer, the byproduct hydrogen ions induce i-motif conformation transformation, metal ions DNAzyme are assembled, cyclic shearing is carried out, and separation and purification of an LAMP complex system and products are achieved; two DNA hairpins marked with electroactive probes methylene blue and ferrocene respectively are triggered on the surface ofthe electrode for directional hybridization without any enzyme intervention, and when the two DNA hairpins are far away from and close to the surface of the electrode respectively, the current signalratio has high-sensitivity and high-specificity target concentration linear correlation. The electrode reaction of the established ratio-type sensor is more simplified, background interference and false positive signals are effectively reduced, the sensitivity is remarkably improved, and a new thought and a technical means are provided for electrochemical detection of DNA of various disease markers.

The invention relates to a monomolecular mechanical method for measuring the influence of CpG adjacent sequences on a protein dissociation time constant. Based on a single-molecule mechanical method,dissociation events of the protein from multiple CpG sites on a same DNA molecule are detected in a high-throughput manner, and the influence of different adjacent sequences on the interaction betweenthe protein and the CpG sites is compared and analyzed. According to the invention, a hairpin structure containing the CpG sites is constructed; a stem part of the structure contains a plurality of CpG sites which are distributed at equal intervals; the CpG adjacent sequences, such as CG, GC, AT and TA, can be set as required, the corresponding CpG sites of the control DNA hairpin structure onlycontain one adjacent sequence, the dissociation time of the protein at each CpG site is compared and analyzed, and how the CpG adjacent sequences influence the protein dissociation time constant can be accurately measured. The method can be used for repeatedly detecting time and probability of the interaction between the protein and different CpG sites in real time in a high-throughput manner to obtain an accurate dissociation time constant, can be widely used for precisely analyzing the interaction between protein and nucleic acid, and is beneficial to life science and medicine research and development.

Doxorubicin (Dox) is widely used for breast cancer treatment but causes serious side-effects including cardiotoxicity that may adversely impact patient lifespan even if treatment is successful. The present invention relates to selective conjugation of Dox to a single site in a DNA hairpin resulting in a highly stable complex that enables Dox to be used more effectively. Selective conjugation of Dox to G15 in the hairpin loop was verified using site-specific labeling with [2-15N]-2′-deoxyguanosine in conjunction with [1H-15N] 2D NMR while 1:1 stoichiometry for the conjugate was validated by ESI-QTOF mass spectrometry and UV spectroscopy. Molecular modeling indicated covalently bound Dox also intercalated into the stem of the hairpin and stability studies demonstrated the resulting Dox-conjugated hairpin (DCH) complex had a half-life >30 h, considerably longer than alternative covalent and non-covalent complexes. Secondary conjugation of DCH with folic acid (FA) resulted in increased internalization into breast cancer cells. The dual conjugate, DCH-FA, can be used for safer and more effective chemotherapy with Dox and this conjugation strategy can be expanded to include additional anti-cancer drugs.

The invention relates to the field of electrochemical biosensors, in particular to a CRISPR (clustered regularly interspaced short palindromic repeats) system of Cpf1 (Cpf1) from bacteria of the hispiraceae, and the electrochemical biosensor for detecting pathogenic bacteria salmonella typhimurium is developed by combining the CRISPR system with a hybrid chain reaction. The autonomous cross opening of the functional DNA hairpin structure of the HCR produces a double-stranded DNA line of a polymer consisting of a plurality of single-stranded DNA, and the plurality of functional single-stranded DNA initiate the trans-cleavage activity of CRISPR-Cas12a, so that the DNA hairpin ring of the MB modified on the surface of the gold electrode is selectively cleaved. The process will cause a change in electron transfer of the electrochemical tag. And the polymer double-stranded DNA of the HCR is fixed on the DBs through a salmonella typhimurium aptamer. The established method can selectively and sensitively quantify salmonella typhimurium in a sample, and the detection limit is 20 CFU/mL.

The invention discloses a power load prediction method for optimizing Elman through GWO based on DNA hairpin variation, an Elman power load prediction model is constructed, parameters of the Elman power load prediction model are optimized by using an improved grey wolf algorithm, and the improved grey wolf algorithm is that DNA hairpin variation is adopted to act on the grey wolf algorithm. The original grey wolf algorithm is improved, molecular biology and a GWO optimization algorithm are combined, a designed mutation operator enables the algorithm to have the capability of jumping out of local optimum, and the convergence speed and the convergence precision are improved; and compared with a static network, the Elman neural network has a relatively strong dynamic mapping characteristic through an internal feedback mechanism, so that the Elman neural network has a relatively strong capability of adapting to a time-varying characteristic, and the power load prediction speed is improved while the prediction precision is met.

The invention discloses a method for determining the content of chloramphenicol based on a biosensor of a glucometer, which comprises the following steps: connecting a specific complementary chain DNA sequence to the surface of a magnetic particle through biotin-avidin interaction, and releasing the specific DNA sequence through the specific action of chloramphenicol and an aptamer, and initiating a cyclic self-assembly process of a hairpin structure modified with gold nanoparticles/amylase, and measuring the content of chloramphenicol by utilizing magnetic separation and collection and combining with a glucometer. The preparation method comprises the following specific steps: S1, synthesizing AuNPs; S2, synthesizing aminated magnetic particles (MNPs); S3, modifying the MNPs (MNPs/tDNA/Aptamer); S4, carrying out DNA hairpin pretreatment (H1 and H2); S5, marking the NDA and glucoamylase (AuNPs/H1/E, AuNPs/H2/E) by using the AuNPs; S6, measuring chloramphenicol based on a glucometer; and S7, selectively investigating the content of chloramphenicol based on a glucometer. According to the method, the problem of measuring the content of chloramphenicol by using a glucometer is solved, and the detection requirement of the public on chloramphenicol is met.