4 results about "Dna hairpin" patented technology

The invention belongs to the technical field of nucleic acid detection and mass spectrometry, and particularly relates to a nucleic acid multiple analysis and detection platform based on full DNA mass coding and normal pressure mass spectrometry detection, and the platform constructs a non-enzyme cascade amplification system completely composed of DNA molecules. A group of DNA hairpin structure chains capable of responding to a target miRNA to be detected to trigger a hybridization chain reaction are designed, a DNA enzyme core with catalytic activity is formed in a product obtained by the hybridization chain reaction of the DNA hairpin structure chains and the target miRNA to be detected, the DNA enzyme can break a substrate chain and release a DNA fragment with characteristic quality coding, and a signal is directly read by virtue of a normal pressure mass spectrum, so that the target miRNA to be detected can be detected. Parallel recognition and quantitative detection of multiple miRNAs are realized; the reaction can be spontaneously completed in a homogeneous aqueous solution system, parallel recognition and accurate quantification of multiple miRNA molecules are realized, and a novel detection method with high compatibility and high sensitivity is provided for high-throughput nucleic acid detection and multi-marker diagnosis.

The application discloses a detection kit based on a colorimetric method and application thereof, and the detection kit comprises two membrane protein recognition chains T1 and T2, two DNA hairpins H1 and H2 required for forming G-quadruplex, hematin required for a color developing reaction, hydrogen peroxide (H2O2) and ABTS. The membrane protein recognition chains T1 and T2 can anchor membrane proteins, and adjacent hybridization occurs when the membrane proteins dimerize, thereby triggering catalytic hairpin self-assembly of H1 and H2 to form a large number of G-quadruplex structures; when H2O2 exists, G-quadruplex / hematin formed by combining G-quadruplex and hematin can catalyze oxidation of ABTS to generate a color developing reaction. The detection kit has the characteristics of high sensitivity, good specificity, low cost, low dependence on equipment and simple operation in the detection of cell membrane protein dimerization, and also has good application performance in cell signal pathway visualization and rapid diagnosis of tumor cells at a bedside.

The invention discloses a preparation method of threose nucleic acid based on a self-pairing primer template. The method comprises the following operation steps: synthesizing a hairpin-shaped DNA template modified by biotin and hypoxanthine, and carrying out annealing treatment to realize template self-pairing; preparing a TNA sequence in a reaction system compatible with TNA extension and TNA cutting by taking the self-pairing DNA hairpin as a template; the TNA product and the template are separated through the magnetic beads, and the DNA template combined with the magnetic beads is recycled, so that the TNA product is recycled in multiple rounds of reactions, and the target TNA product is prepared in batches; the molecular weight of the product is detected through electrospray ionization mass spectrometry, and the prepared TNA is determined as an expected product. The invention provides a method for rapidly synthesizing a large amount of TNA sequences, the preparation period of TNA is shortened, and technical support is provided for application of TNA in the fields of biotechnology and biomedicine.

The application discloses a transcytosis DNA nanoframework material, a preparation method and application thereof, and the transcytosis DNA nanoframework material is assembled through a hybridization chain reaction of a DNA-containing polymer as a skeleton and DNA hairpins; the skeleton is prepared from at least acrylamide-modified DNA, diethylaminoethyl methacrylate and 3-methacrylamidophenylboronic acid as raw materials through polymerization and oxidation. The transcytosis DNA nanoframework material integrates transcytosis function and gene silencing function, and can be used for precise delivery and treatment of pancreatic cancer and other solid tumors rich in dense matrix barriers.