Ratio type near-infrared II-region fluorescent probe as well as construction method and application thereof

A fluorescent probe and construction method technology, applied in the field of biomedicine, can solve problems such as invasive detection, infection, and large subjective influence, achieve high-specificity staging, achieve precise positioning, and reduce the effect of error and missed detection ratio

Pending Publication Date: 2020-09-11
苏州影睿光学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] SLNB and clinical pathological testing have the following problems: 1) Invasive testing, leading to potential risks of metastasis and infection; 2) The preparation and reading of pathological samples is time-consuming and labor-intensive, and has a large subjective impact; 3) Tumors are heterogeneous , limited puncture cannot obtain complete three-dimensional histopathological information, which is likely to cause missed detection

Method used

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  • Ratio type near-infrared II-region fluorescent probe as well as construction method and application thereof
  • Ratio type near-infrared II-region fluorescent probe as well as construction method and application thereof
  • Ratio type near-infrared II-region fluorescent probe as well as construction method and application thereof

Examples

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Embodiment 1

[0077] 1. Preparation of a ratiometric near-infrared region II fluorescent nanoprobe

[0078] 1) Preparation of lead sulfide quantum dots: Weigh 0.3mmol (0.834g) of lead chloride and 7.5mL (6.1g) of oleylamine into a 100mL three-necked flask, and continuously evacuate with a vacuum pump at room temperature under continuous stirring 5 minutes to remove part of the oxygen and water vapor in the reaction system to prevent Pb 2+ It is oxidized during the subsequent heating process, then filled with nitrogen, heated to 100°C at a temperature increase rate of 5°C / min under the protection of nitrogen atmosphere, and kept at this temperature and continuously vacuumed for 10 minutes to remove as much oxygen and water vapor in the reaction system as possible , continue to feed nitrogen, under the protection of nitrogen atmosphere, the temperature is raised to 120° C. and kept at this temperature for 30 minutes to obtain a viscous white lead precursor. Afterwards, 2.25 mL of sulfur prec...

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Abstract

The invention discloses a ratio type near-infrared II-region fluorescent probe as well as a construction method and application thereof. The ratio type near-infrared II region fluorescent probe comprises a DNA hairpin structure serving as a breast cancer specific miRNA recognition and signal amplification unit; a fluorescence donor and a fluorescence acceptor used as signal reporting units and modifying on the DNA hairpin structure, wherein the fluorescence donor comprises double-emission quantum dots of which the emission wavelength is in a near-infrared II region waveband; a drug molecule loaded on the DNA hairpin structure; and a cell capsule derived from a biological cell as a targeted delivery vehicle. By means of the ratio type fluorescent nanoprobe, in-situ, real-time, high-sensitivity and high-specificity breast cancer staging and typing evaluation can be achieved.

Description

technical field [0001] The invention relates to a near-infrared II region fluorescent probe, in particular to a ratio-type near-infrared II region fluorescent probe for staging and typing of breast cancer, its construction method and application, and belongs to the technical field of biomedicine. Background technique [0002] Patients with breast tumors rank first among female malignant tumors in China and even in the world. The incidence rate is increasing at a rate of 2%-3% per year, and it is showing a younger trend, which greatly threatens women's physical and mental health. Whether it is traditional surgery, chemotherapy and radiotherapy, or endocrine therapy and targeted therapy, the clinical staging and typing of breast cancer are very important. Tissue biopsy and pathological analysis have always played an irreplaceable important role in the study of tumor staging and typing and clinical practice. [0003] The TNM staging system is currently the most commonly used t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B5/00
CPCA61B5/004A61B5/0071A61B5/0091
Inventor 王强斌李春炎
Owner 苏州影睿光学科技有限公司
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