278 results about "Biology cell" patented technology

The invention relates to the field of biological cells, and particularly discloses a method for quantitatively analyzing metabolites in biological cells based on a Raman spectrum technology, and the method comprises the following steps: S1, collecting a Raman spectrum data set; s2, metabolite with uniform distribution and metabolite with stable content in the cells to be compared are selected as internal standards; s3, extracting the Raman characteristic peak area of the target object, and performing Raman distribution imaging in the cells according to the Raman characteristic peak area of the target object to obtain Raman imaging pictures of a plurality of substances; s4, performing Raman imaging normalization on the target object; s5, converting the normalized Raman peak intensity of the target object into a thermal image, and presenting Raman signals with different intensities in different colors to obtain an intracellular distribution diagram of each component of the intracellular metabolite; and S6, spatial distribution and quantitative analysis of intracellular metabolites. The method disclosed by the invention has the advantages of in-situ, nondestructive, high speed, quick effect, capability of simultaneously and quantitatively analyzing various metabolites on the subcellular scale and the like.

In one embodiment, a method is disclosed to determine one or more biological cells of interest to identify and count in a mixed biological sample fluid with differing biological cells. The method can include selecting cell markers associated for biological cells to which conjugated antibodies can attach with differing fluorescent dyes; displaying a panel builder graphical user interface window to display a co-expression matrix by biological cell type to assist in selecting cell markers to assign co-expression; and selecting cell markers to assign co-expression with an input device.

The invention provides a Burkholderia paradensis (Paraburkholderia caledonica) and an application of the Burkholderia paradonica (Paraburkholderia caledonica). The strain provided by the invention can realize green and efficient recovery of various rare earth raw materials. According to the present invention, the bacterial strain can produce a variety of organic acids and secondary metabolites having complexing properties during the metabolism process, and the electron transfer process on the surface of the microbial cell membrane provides important effects in the leaching of different rare earth raw materials;

The invention discloses a filtering and sorting device for biological cell culture and a use method of the filtering and sorting device, and belongs to the technical field of biological cell culture.The filtering and sorting device is provided with a first arc-shaped connecting block, a sealing ring, an arc-shaped connecting clamping plate, a clamping spring, a fixing clamping column, a guide cylinder and a top cover, and the arc-shaped connecting clamping plate is tightly attached to the surface of the sealing ring; meanwhile, a push plate is pushed to drive a fixed clamping column to move, so that a clamping spring is extruded and deformed on the surface of a mounting column, after an arc-shaped connecting clamping plate is tightly attached to the surface of the sealing ring, the fixed clamping column corresponds to clamping grooves in the two sides of an I-shaped clamping block in position, and the push plate is loosened to jack up the fixed clamping column to be clamped into the clamping grooves in the two sides of the I-shaped clamping block under the rebound effect of the clamping spring; and therefore, the concave mounting blocks are clamped and fixed to the two sides of the I-shaped clamping blocks, the arc-shaped connecting clamping plates are tightly attached and fixed to the surface of the sealing ring to connect and fix the collecting barrel and the top cover, and therefore the collecting barrel, the sealing ring and the top cover can be conveniently connected and fixed or detached and cleaned, and the flow guide barrel can be detached and replaced.

The invention discloses a rapid biological cell classification imaging method and system based on a quantum correlation diffraction optical neural network, and the method comprises the steps: regulating and controlling a signal photon to an orbital angular momentum state optimized by a neural network based on a time-energy correlation two-photon pair generated in a spontaneous parametric down-conversion process, and irradiating a biological cell sample; a diffractive optical neural network is utilized to construct a quantum state illumination diffractive optical processor, photons carrying cell complex amplitude information are mapped to different detection areas of a predefined image plane, and rapid and high-precision cell classification and recognition are realized through correlation imaging results of an enhanced charge coupled camera. The method has the signal-to-noise ratio and anti-interference capability exceeding the classic limit, can keep high-precision cell classification capability under high background noise, and is suitable for long-time monitoring of living cells under the condition of extremely low light intensity. The method has the characteristics of low energy consumption and high efficiency, and meets the requirements of green calculation and sustainable development. And the method can be used for rapidly classifying and identifying the living biological cells without marks and phototoxicity.

Embodiments of the present invention may provide protecting, preserving, and even repairing biological cells during in vitro processing. A uniform environment such as a protective layer with exogeneous lipids may be formed around biological cells which may interact with the plasma membranes of the cells.

A monitoring device of biological cells including: a fluidic channel in which a fluid including biological cells is made to flow, including a first and second glass walls placed in parallel and a reflective surface adjoined to the second wall; and a piezoelectric transducer, the piezoelectric transducer emitting acoustic waves creating a force acting on the biological cells, making them flow substantially along a single motion plane. The external interface between the first wall and the exterior of the fluidic channel and the reflective surface are configured to function as a Fabry-Perot interferometer, such that the motion planes sensibly perpendicular to the optical axis of the Fabry-Perot interferometer. The finesse of the interferometer is lower than 10. The monitoring device further includes a detecting unit detecting a fringe pattern stemming from the Fabry-Perot interferometer and an analysing device, analysing the fringe pattern and configured to output data on physical properties of the cells based on the pattern.

A recombinant microbial cell is capable of producing at least one compound having structural Formula II from a carbon source:In Formula II, n=2-3; R1=H or COCH3 or CH2CH2COX with X=OH or O—; R2=CH3 or CH2CH2COX with X=OH or O— where the cell comprises a genetic modification to increase activity relative to its wild-type cell of E4 where E4 is a desferrioxamine or bisucaberin synthetase (EC 6.3.-.-) (E4i) capable of converting N5-aminopentyl-N-(hydroxy)-succinamic acid to desferrioxamine B or H or at least one other linear desferrioxamine or bisucaberin according to Formula II.

The present application relates to the technical field of biological cell culture, and particularly relates to a three-dimensional cell culture device with automatic liquid replacement function, comprising: a box body; a rotating assembly, a liquid replacement assembly and a control display assembly are integrally installed in the box body. The three-dimensional cell culture device with automatic liquid replacement function provided by the present application integrates microgravity simulation and liquid replacement function in a relatively closed culture box body space, ensures that the culture environment in the cell culture process has high stability by not excessively affecting the air flow, temperature and humidity environment, etc. in the culture box body during liquid replacement; the device for replacing culture solution does not need to rotate, effectively releases the culture space while reducing the energy consumption required in the rotation process, can load multiple culture bottles for three-dimensional culture and automatic liquid replacement at the same time, and has high potential for large-scale cell preparation.

It can be useful to regulate the growth of microbial cells. Some embodiments herein provide genetically engineered microbial cells that can produce bacteriocins to control the growth of microbial cells. In some embodiments, microbial cells are contained within a desired environment. In some embodiments, contaminating microbial cells are neutralized. In some embodiments, a first microbial cell type regulates the growth of a second microbial cell type so as to maintain a desired ratio of the two cell types.

The invention provides a biological cell freezing visualization system and method based on a metal woven belt, and relates to the technical field of biologication.The biological cell freezing visualization system comprises a bottom plate, a support platform, a profound hypothermia liquid tank and an observation assembly are fixedly connected to the upper surface of the bottom plate, a lifting sliding table is fixedly connected to the inner bottom face of the support platform, and a constant hypothermia copper table is fixedly connected to the upper end of the lifting sliding table; the constant hypothermia copper table is communicated with the deep hypothermia liquid tank through a liquid conveying hose, the inner top surface of the support platform is fixedly connected with a woven belt fixing support and a sample placing platform, one end of a metal woven belt is fixedly connected to the lower surface of the woven belt fixing support, and the other end of the metal woven belt is fixedly connected to the lower surface of the sample placing platform; a cover plate covers the sample placing platform, and the observation assembly is used for shooting an image of the sample placing platform. Cold is conducted through the metal woven belt, the requirement for the movement precision of the device is low, the durability of the device is improved, the position of the sample containing platform is fixed, and it is guaranteed that imaging of the observation assembly is clear.

A method of producing hematopoietic stem / progenitor cells (HS / PCs) and blood cells from adipose derived mesenchymal stem cells (Ad MSCs). The method comprises obtaining biological cells of a subject; expanding the biological cells; obtaining a pure Ad MSCs cell line from the expanded biological cells; converting the pure Ad MSCs cell line into HS / PCs in vitro; expanding the converted HS / PCs in vitro; and obtaining the expanded HS / PCs.

The invention relates to the technical field of biological cell culture media, and discloses a culture medium additive and a culture medium capable of improving the cell caking condition in the CHO cell culture process, and a method for culturing CHO cells in vitro by using the culture medium. According to the serum-free culture medium for culturing the CHO cells, calcium, magnesium and phosphorus substances with the original concentration and calcium, magnesium and phosphorus with the adjusted concentration are additionally added, the culture medium is optimized, the cell caking condition in the CHO cell culture process is improved, and the serum-free culture medium for culturing the CHO cells is simple in component, low in cost and free of protein and serum. When the optimized culture medium is used for culturing to the seventh day, the cell caking rate can be reduced from 37.15% to 18.38%, and the cell density can be increased from 4.56 * 10 < 6 > cells / mL to 17.4 * 10 < 6 > cells / mL.

A method for electroporation of biological cells, comprising the steps of: providing a shock tube for cell electroporation; filling up the cavity with the cell sample comprising cells and materials to be injected into the cells, wherein a bulged liquid surface is formed; securing the stopple to a second end of the tube by a positioning structure, while generating a compressive deformation to the elastic piece between the stopple and the second electrode; connecting the first electrode and the second electrode with a pulse power supply to perform electroporation.

The invention discloses a biological cell culture sampling device for a biological test, which comprises a culture bottle and a sealing cover made of a transparent material, a rotary locking mechanism and a sealing assembly are arranged between the sealing cover and a sampling port in a matched manner, and an electric push rod is fixedly arranged at the upper end of the sealing cover. The piston end of the electric push rod extends into the sealing cover and is fixedly provided with a sampling needle assembly, the side wall of the sealing cover is communicated with a vacuumizing connector and a pressure regulating air inlet valve, an efficient particle air filtering film is arranged in the pressure regulating air inlet valve, and the top of the sealing cover is provided with a suction connector used for being connected with external negative pressure suction equipment. Through cooperation of the rotary locking mechanism and the sealing assembly, reliable sealing of the sealing cover and the sampling opening of the culture bottle is achieved, vacuumizing pretreatment is combined, external air is prevented from entering the culture bottle during puncture sampling, the sterility of a culture system is guaranteed, a main sealing structure of the culture bottle does not need to be opened in the whole sampling process, and the sampling efficiency is improved. And the original stable gas environment in the culture bottle is maintained.

The invention discloses a dual-channel fluorescent probe for simultaneously detecting nitric oxide and cysteine as well as a preparation method and application of the dual-channel fluorescent probe. The structural formula of the dual-channel fluorescent probe is shown in the specification. The invention also specifically discloses a preparation method of the dual-channel fluorescent probe and application of the dual-channel fluorescent probe in selective detection of NO and Cys, dynamic visual detection of NO and Cys in a biological cell system and in ferroptosis and depression cell models. The dual-channel fluorescent probe disclosed by the invention has the advantages of strong specificity, good selectivity, no spectrum crosstalk and the like.

Devices for filtering liquids are provided herein. More particularly, the devices may be devices for separating biological cells from microcarriers the cells have been grown with, as well as other debris and materials, that are found in the cell culturing liquid. The devices may also be foldable or collapsible.

A system and method of categorizing protein expression in biological cells may include receiving a Whole Slide Image (WSI) of an Immunohistochemistry (IHC) assay of cells, stained with two or more color-specific markers, of two or more respective proteins, and applying a stain separation algorithm on the WSI image, to obtain two or more single¬ channel marker images. Based on the two or more marker images, embodiments may generate an enhanced color image, representing expression of the two or more proteins in the assay, and prompt a user to assign at least one label to at least one respective location in the enhanced color image. Embodiments may then use the at least one label as supervisory information, to train a machine-learning (ML)-based classification model, to classify at least one cell -representative location according to categories of expression of the at least two proteins.

This invention relates to the field of ion-conducting membrane technology, and particularly to a wedge-shaped amphiphilic molecule, a biomimetic ion-conducting membrane, and its preparation method. The biomimetic ion-conducting membrane utilizes the self-assembly behavior of the wedge-shaped amphiphilic molecule to form a columnar mesocrystalline phase containing transport channels. This columnar structure is fixed within a membrane framework composed of methacrylate polymers using ultraviolet graft copolymerization to form an ion-transfer membrane. This membrane exhibits strong ion selectivity and conductivity, and its mechanism of action is similar to that of biological cell membranes. It can be used for the transport and separation of microparticle materials such as ions, small molecules, and micro-electronic conductors, showing broad application prospects in fields such as biology, medicine, and environmental analysis. Furthermore, its ion conductivity and chemical stability also demonstrate its potential application in proton exchange membrane fuel cells.

The present invention provides a system and method of labeling a biological cell by at least one processor. Embodiments include obtaining a transmitted-light microscopy patch depicting the biological cell, extracting a context vector representing biological characteristics based on the microscopy patch, processing the patch through a machine-learning encoder to generate a feature vector representing the cell in a latent feature space, employing a machine-learning transformation model to augment the feature vector based on the context vector, and applying a generative decoder model on the augmented feature vector to predict at least one organelle image, wherein the predicted organelle image imitates an organelle-specific fluorescent image of the biological cell. Embodiments may further include presenting the predicted organelle image via a user interface, enabling a human expert to determine a state of the biological cell and / or biological sample based on the transmitted-light microscopy patch.

Disclosed are methylglyoxal resistant genetically engineered microbial cells for the fermentative production of an oligosaccharide of interest, and methods for fermentative production of an oligosaccharide of interest using the methylglyoxal resistant microbial cells.

The application relates to a kind of mitochondria-based single cell feature extraction and analysis method and system, comprising: obtaining the multiple modal images such as bright field image, nucleus fluorescent image and mitochondria fluorescent image of single cell;Image preprocessing is carried out to the three modal images obtained;For different structures such as mitochondria, morphological and texture features are extracted, and feature analysis is carried out;Further, through the fusion of mitochondria and machine learning technology, the automatic classification of cells is realized.The application is used for the classification of human cervical epithelial cells (H8) and cervical cancer cells (HeLa), and the machine learning analysis of morphological features and texture features shows the potential of mitochondria in the classification of cervical cells.The application has strong applicability, can be combined with machine learning and other analysis methods, and can be applied to various biological cells, has universality, and is easy to popularize.

A method for producing a nutritional supplement from microbial cells is disclosed. The method includes culturing microbial cells to obtain a biomass, incubating the biomass by heat treatment at a temperature between 55° C. and 80° C. for an incubation time between 10 and 60 minutes, and concentrating the biomass by separating and removing the liquid phase from the solid phase to obtain a dry matter content between 2% and 40% of the total weight of the nutritional supplement. A system for producing a nutritional supplement from microbial cells using the aforementioned method is also disclosed. The system includes a bioreactor, a heat exchanger, and a separator for carrying out each step of the aforementioned method.

An electrode unit for performing electroporation of a biological cell is provided, including at least one electrode which can contact a cell. A stimulation unit and an impedance measurement device are connected to the cell, respectively to provide signals to provide cell electroporation and to measure the impedance of the electrode in contact with the cell. Further, a memory stores a predetermined value of an impedance parameter of the biological cell, and it is arranged to be readable by a comparing element. The comparing element is configured to compare the value stored in the memory with a value measured with the impedance measurement device, and to produce an adjustment signal to the stimulation unit, forming a feedback loop. The unit is further configured to apply a further electrical signal for providing electroporation of the cell upon receiving the adjustment signal from the comparing element.

According to an aspect of the present inventive concept there is provided a device for sorting cells comprising: a flow channel, the flow channel comprises at least one zone associated with a cell category, configured to modify movement of the biological cell in dependence of the cell belonging to the cell category; the flow channel comprises a sorting channel having a width for presenting the cells in a queue one-by-one; the device comprises a first detector configured to detect a cell exhibiting at least one modified movement; the device comprising a second detector for verifying an order of the cells in the queue or detecting a change in the order of the cells in the queue; the device comprises a sorting element configured to act on the biological cells in the queue, to selectively affect a cell of the queue based on the order of the cells and the classification of the cell.

The invention discloses culture equipment for biological cell tissues, and relates to the technical field of biological cell culture. The device comprises a culture box and a rotating shaft, the rotating shaft is rotatably installed in the culture box, a box door is fixedly installed on the rotating shaft, a transmission mechanism is arranged on the rotating shaft, the transmission mechanism is meshed with a rack, a connecting rod is fixedly installed on the rack, the connecting rod is tightly attached to an air rod, the air rod is slidably connected into an air cylinder, and an air guide pipe is arranged at the front end of the air cylinder. A one-way control valve is installed at the front end of the air guide pipe, the front end of the air guide pipe is communicated with a disinfection water tank, the front side of the disinfection water tank is communicated with a pressure valve pipe, the lower end of the pressure valve pipe is communicated with a splitter plate, and a rotating shaft is arranged in the incubator. By opening the box door, the splitter plate can automatically spray vaporific disinfectant fluid downwards, and the vaporific disinfectant fluid can form an atomized protective layer of the disinfectant fluid, so that bacteria or impurities in the external environment are prevented from entering the incubator after the box door is opened.

The application discloses a kind of preparation and application of microbial basic protein and adhesive proteoglycan hydrogel, belong to the field of biomaterials.Basic protein is extracted from microbial cells in the present application, using supramolecular assembly method, basic protein and acidic polysaccharide electrostatic assembly protein glycan gel material with strong tissue adhesion, the method is simple, widely available, and the cost is almost negligible.The tissue adhesive provided by the present application has immediate tissue adhesion performance, excellent biocompatibility and excellent antibacterial ability and hemostatic performance, which is beneficial to cope with different types of bleeding scene and irregular wound healing.Through cell compatibility and blood compatibility test, the results show that the material has excellent biocompatibility, and the basic protein has super strong bacteriostatic ability, avoids the invasion of foreign bacteria in the wound repair process, and has strong healing ability, greatly shortens the healing period of wound.

The invention provides a metal boride nano particle for diagnosing and treating cancer, the metal boride nano particle has dual functions of diagnosing and treating tumor, the surface of the metal boride nano particle is modified with an antibody and a biological probe or is coated with a biological cell membrane, and the antibody or the probe has specificity of being combined with a receiver of a specific tumor cell.

A method is proposed for detecting at least one fluorescence pattern on an immunofluorescence image of a biological cell substrate, comprising the following steps: incubating the cell substrate with a liquid patient sample, which potentially includes primary antibodies, and furthermore with secondary antibodies, which are marked using a fluorescence stain, irradiating the cell substrate using excitation radiation and capturing the immunofluorescence image, determining segmentation information comprising at least one first and one second segmentation area, wherein the segmentation areas each represent a respective cell substrate area, via segmentation of the immunofluorescence image using a first neural network, determining a boundary area, which represents a transition from the first cell substrate area towards the second cell substrate area in the fluorescence image, on the basis of the segmentation information, selecting multiple partial images from the immunofluorescence image along the boundary area, determining a confidence measure of a presence of the fluorescence pattern on the basis of the multiple partial images via a second neural network.