179 results about "Biology cell" patented technology

In one embodiment, a method is disclosed to determine one or more biological cells of interest to identify and count in a mixed biological sample fluid with differing biological cells. The method can include selecting cell markers associated for biological cells to which conjugated antibodies can attach with differing fluorescent dyes; displaying a panel builder graphical user interface window to display a co-expression matrix by biological cell type to assist in selecting cell markers to assign co-expression; and selecting cell markers to assign co-expression with an input device.

The invention discloses a rapid biological cell classification imaging method and system based on a quantum correlation diffraction optical neural network, and the method comprises the steps: regulating and controlling a signal photon to an orbital angular momentum state optimized by a neural network based on a time-energy correlation two-photon pair generated in a spontaneous parametric down-conversion process, and irradiating a biological cell sample; a diffractive optical neural network is utilized to construct a quantum state illumination diffractive optical processor, photons carrying cell complex amplitude information are mapped to different detection areas of a predefined image plane, and rapid and high-precision cell classification and recognition are realized through correlation imaging results of an enhanced charge coupled camera. The method has the signal-to-noise ratio and anti-interference capability exceeding the classic limit, can keep high-precision cell classification capability under high background noise, and is suitable for long-time monitoring of living cells under the condition of extremely low light intensity. The method has the characteristics of low energy consumption and high efficiency, and meets the requirements of green calculation and sustainable development. And the method can be used for rapidly classifying and identifying the living biological cells without marks and phototoxicity.

Embodiments of the present invention may provide protecting, preserving, and even repairing biological cells during in vitro processing. A uniform environment such as a protective layer with exogeneous lipids may be formed around biological cells which may interact with the plasma membranes of the cells.

The present application relates to the technical field of biological cell culture, and particularly relates to a three-dimensional cell culture device with automatic liquid replacement function, comprising: a box body; a rotating assembly, a liquid replacement assembly and a control display assembly are integrally installed in the box body. The three-dimensional cell culture device with automatic liquid replacement function provided by the present application integrates microgravity simulation and liquid replacement function in a relatively closed culture box body space, ensures that the culture environment in the cell culture process has high stability by not excessively affecting the air flow, temperature and humidity environment, etc. in the culture box body during liquid replacement; the device for replacing culture solution does not need to rotate, effectively releases the culture space while reducing the energy consumption required in the rotation process, can load multiple culture bottles for three-dimensional culture and automatic liquid replacement at the same time, and has high potential for large-scale cell preparation.

It can be useful to regulate the growth of microbial cells. Some embodiments herein provide genetically engineered microbial cells that can produce bacteriocins to control the growth of microbial cells. In some embodiments, microbial cells are contained within a desired environment. In some embodiments, contaminating microbial cells are neutralized. In some embodiments, a first microbial cell type regulates the growth of a second microbial cell type so as to maintain a desired ratio of the two cell types.

The invention provides a biological cell freezing visualization system and method based on a metal woven belt, and relates to the technical field of biologication.The biological cell freezing visualization system comprises a bottom plate, a support platform, a profound hypothermia liquid tank and an observation assembly are fixedly connected to the upper surface of the bottom plate, a lifting sliding table is fixedly connected to the inner bottom face of the support platform, and a constant hypothermia copper table is fixedly connected to the upper end of the lifting sliding table; the constant hypothermia copper table is communicated with the deep hypothermia liquid tank through a liquid conveying hose, the inner top surface of the support platform is fixedly connected with a woven belt fixing support and a sample placing platform, one end of a metal woven belt is fixedly connected to the lower surface of the woven belt fixing support, and the other end of the metal woven belt is fixedly connected to the lower surface of the sample placing platform; a cover plate covers the sample placing platform, and the observation assembly is used for shooting an image of the sample placing platform. Cold is conducted through the metal woven belt, the requirement for the movement precision of the device is low, the durability of the device is improved, the position of the sample containing platform is fixed, and it is guaranteed that imaging of the observation assembly is clear.

A method of producing hematopoietic stem / progenitor cells (HS / PCs) and blood cells from adipose derived mesenchymal stem cells (Ad MSCs). The method comprises obtaining biological cells of a subject; expanding the biological cells; obtaining a pure Ad MSCs cell line from the expanded biological cells; converting the pure Ad MSCs cell line into HS / PCs in vitro; expanding the converted HS / PCs in vitro; and obtaining the expanded HS / PCs.

The invention discloses a biological cell culture sampling device for a biological test, which comprises a culture bottle and a sealing cover made of a transparent material, a rotary locking mechanism and a sealing assembly are arranged between the sealing cover and a sampling port in a matched manner, and an electric push rod is fixedly arranged at the upper end of the sealing cover. The piston end of the electric push rod extends into the sealing cover and is fixedly provided with a sampling needle assembly, the side wall of the sealing cover is communicated with a vacuumizing connector and a pressure regulating air inlet valve, an efficient particle air filtering film is arranged in the pressure regulating air inlet valve, and the top of the sealing cover is provided with a suction connector used for being connected with external negative pressure suction equipment. Through cooperation of the rotary locking mechanism and the sealing assembly, reliable sealing of the sealing cover and the sampling opening of the culture bottle is achieved, vacuumizing pretreatment is combined, external air is prevented from entering the culture bottle during puncture sampling, the sterility of a culture system is guaranteed, a main sealing structure of the culture bottle does not need to be opened in the whole sampling process, and the sampling efficiency is improved. And the original stable gas environment in the culture bottle is maintained.

The invention discloses a dual-channel fluorescent probe for simultaneously detecting nitric oxide and cysteine as well as a preparation method and application of the dual-channel fluorescent probe. The structural formula of the dual-channel fluorescent probe is shown in the specification. The invention also specifically discloses a preparation method of the dual-channel fluorescent probe and application of the dual-channel fluorescent probe in selective detection of NO and Cys, dynamic visual detection of NO and Cys in a biological cell system and in ferroptosis and depression cell models. The dual-channel fluorescent probe disclosed by the invention has the advantages of strong specificity, good selectivity, no spectrum crosstalk and the like.

Devices for filtering liquids are provided herein. More particularly, the devices may be devices for separating biological cells from microcarriers the cells have been grown with, as well as other debris and materials, that are found in the cell culturing liquid. The devices may also be foldable or collapsible.

A system and method of categorizing protein expression in biological cells may include receiving a Whole Slide Image (WSI) of an Immunohistochemistry (IHC) assay of cells, stained with two or more color-specific markers, of two or more respective proteins, and applying a stain separation algorithm on the WSI image, to obtain two or more single¬ channel marker images. Based on the two or more marker images, embodiments may generate an enhanced color image, representing expression of the two or more proteins in the assay, and prompt a user to assign at least one label to at least one respective location in the enhanced color image. Embodiments may then use the at least one label as supervisory information, to train a machine-learning (ML)-based classification model, to classify at least one cell -representative location according to categories of expression of the at least two proteins.

This invention relates to the field of ion-conducting membrane technology, and particularly to a wedge-shaped amphiphilic molecule, a biomimetic ion-conducting membrane, and its preparation method. The biomimetic ion-conducting membrane utilizes the self-assembly behavior of the wedge-shaped amphiphilic molecule to form a columnar mesocrystalline phase containing transport channels. This columnar structure is fixed within a membrane framework composed of methacrylate polymers using ultraviolet graft copolymerization to form an ion-transfer membrane. This membrane exhibits strong ion selectivity and conductivity, and its mechanism of action is similar to that of biological cell membranes. It can be used for the transport and separation of microparticle materials such as ions, small molecules, and micro-electronic conductors, showing broad application prospects in fields such as biology, medicine, and environmental analysis. Furthermore, its ion conductivity and chemical stability also demonstrate its potential application in proton exchange membrane fuel cells.

The present invention provides a system and method of labeling a biological cell by at least one processor. Embodiments include obtaining a transmitted-light microscopy patch depicting the biological cell, extracting a context vector representing biological characteristics based on the microscopy patch, processing the patch through a machine-learning encoder to generate a feature vector representing the cell in a latent feature space, employing a machine-learning transformation model to augment the feature vector based on the context vector, and applying a generative decoder model on the augmented feature vector to predict at least one organelle image, wherein the predicted organelle image imitates an organelle-specific fluorescent image of the biological cell. Embodiments may further include presenting the predicted organelle image via a user interface, enabling a human expert to determine a state of the biological cell and / or biological sample based on the transmitted-light microscopy patch.

Disclosed are methylglyoxal resistant genetically engineered microbial cells for the fermentative production of an oligosaccharide of interest, and methods for fermentative production of an oligosaccharide of interest using the methylglyoxal resistant microbial cells.

The application relates to a kind of mitochondria-based single cell feature extraction and analysis method and system, comprising: obtaining the multiple modal images such as bright field image, nucleus fluorescent image and mitochondria fluorescent image of single cell;Image preprocessing is carried out to the three modal images obtained;For different structures such as mitochondria, morphological and texture features are extracted, and feature analysis is carried out;Further, through the fusion of mitochondria and machine learning technology, the automatic classification of cells is realized.The application is used for the classification of human cervical epithelial cells (H8) and cervical cancer cells (HeLa), and the machine learning analysis of morphological features and texture features shows the potential of mitochondria in the classification of cervical cells.The application has strong applicability, can be combined with machine learning and other analysis methods, and can be applied to various biological cells, has universality, and is easy to popularize.

A method for producing a nutritional supplement from microbial cells is disclosed. The method includes culturing microbial cells to obtain a biomass, incubating the biomass by heat treatment at a temperature between 55° C. and 80° C. for an incubation time between 10 and 60 minutes, and concentrating the biomass by separating and removing the liquid phase from the solid phase to obtain a dry matter content between 2% and 40% of the total weight of the nutritional supplement. A system for producing a nutritional supplement from microbial cells using the aforementioned method is also disclosed. The system includes a bioreactor, a heat exchanger, and a separator for carrying out each step of the aforementioned method.

An electrode unit for performing electroporation of a biological cell is provided, including at least one electrode which can contact a cell. A stimulation unit and an impedance measurement device are connected to the cell, respectively to provide signals to provide cell electroporation and to measure the impedance of the electrode in contact with the cell. Further, a memory stores a predetermined value of an impedance parameter of the biological cell, and it is arranged to be readable by a comparing element. The comparing element is configured to compare the value stored in the memory with a value measured with the impedance measurement device, and to produce an adjustment signal to the stimulation unit, forming a feedback loop. The unit is further configured to apply a further electrical signal for providing electroporation of the cell upon receiving the adjustment signal from the comparing element.

According to an aspect of the present inventive concept there is provided a device for sorting cells comprising: a flow channel, the flow channel comprises at least one zone associated with a cell category, configured to modify movement of the biological cell in dependence of the cell belonging to the cell category; the flow channel comprises a sorting channel having a width for presenting the cells in a queue one-by-one; the device comprises a first detector configured to detect a cell exhibiting at least one modified movement; the device comprising a second detector for verifying an order of the cells in the queue or detecting a change in the order of the cells in the queue; the device comprises a sorting element configured to act on the biological cells in the queue, to selectively affect a cell of the queue based on the order of the cells and the classification of the cell.

The application discloses a kind of preparation and application of microbial basic protein and adhesive proteoglycan hydrogel, belong to the field of biomaterials.Basic protein is extracted from microbial cells in the present application, using supramolecular assembly method, basic protein and acidic polysaccharide electrostatic assembly protein glycan gel material with strong tissue adhesion, the method is simple, widely available, and the cost is almost negligible.The tissue adhesive provided by the present application has immediate tissue adhesion performance, excellent biocompatibility and excellent antibacterial ability and hemostatic performance, which is beneficial to cope with different types of bleeding scene and irregular wound healing.Through cell compatibility and blood compatibility test, the results show that the material has excellent biocompatibility, and the basic protein has super strong bacteriostatic ability, avoids the invasion of foreign bacteria in the wound repair process, and has strong healing ability, greatly shortens the healing period of wound.

A method is proposed for detecting at least one fluorescence pattern on an immunofluorescence image of a biological cell substrate, comprising the following steps: incubating the cell substrate with a liquid patient sample, which potentially includes primary antibodies, and furthermore with secondary antibodies, which are marked using a fluorescence stain, irradiating the cell substrate using excitation radiation and capturing the immunofluorescence image, determining segmentation information comprising at least one first and one second segmentation area, wherein the segmentation areas each represent a respective cell substrate area, via segmentation of the immunofluorescence image using a first neural network, determining a boundary area, which represents a transition from the first cell substrate area towards the second cell substrate area in the fluorescence image, on the basis of the segmentation information, selecting multiple partial images from the immunofluorescence image along the boundary area, determining a confidence measure of a presence of the fluorescence pattern on the basis of the multiple partial images via a second neural network.

The invention relates to a biosensor (100) for detecting biological cells in a sample. The biosensor (100) comprises a plurality of first conductor strips (110) arranged parallel to one another, each of which can be individually connected to a first electrical potential. The biosensor (100) also comprises a plurality of second conductor strips (120) arranged parallel to one another, each of which can be individually connected to a second electrical potential, wherein the first conductor strips (110) and the second conductor strips (120) intersect at a plurality of intersection points. The biosensor (100) further comprises an insulating layer structure (140), which is arranged between the first conductor strips (110) on the one hand and the second conductor strips (120) on the other.The biosensor (100) also comprises a plurality of depression sections (150) which are formed in the insulator layer structure (140) on the side of the second conductor strips (120) in the area of ​​the intersection points. Each depression section (150) is designed to couple with at least one biological recognition element (X) capable of binding to biological cells, in order to immobilize and detect a maximum of one biological cell in the area of ​​each depression section (150).

The present application relates to the technical field of biological cell culture, and particularly relates to a fixed bed device and method for improving secretion and expression of exosomes. The device comprises a fixed bed reactor, an ultrasonic generator, a connecting flange and a control unit. The ultrasonic generator is installed on the top of the fixed bed reactor through the connecting flange. The control unit is configured to control the ultrasonic generator to apply ultrasonic stimulation to the cells intermittently in the whole cycle of cell culture. The ultrasonic intensity is dynamically regulated in three stages according to the culture cycle. The present application uses pure physical stimulation without adding chemical reagents. Under the premise of maintaining normal growth and activity of cells, the yield of exosomes can be increased by more than 169%. The present application is suitable for animal cells and plant cells and has a wide industrial application prospect. The technical problems of low yield of exosomes, stimulation mode that may affect cell activity or introduction of chemical reagents that may interfere in the prior art can be effectively solved.

The application relates to a biological cell culture device and a culture method, and relates to the technical field of cell culture. The device comprises a culture box main body with an open upper side. A placing mechanism and a pressing mechanism are arranged in the culture box main body. Before a cylinder is removed, the upper end of the cylinder is first sealed by a sealing plate. Therefore, after the culture dish is removed, external air cannot enter the culture box main body from the corresponding cylinder. When the subsequent culture dish enters, only the gas with the same volume as the cylinder enters the box, and the influence on the gas concentration in the culture box main body is not obvious. The gas concentration environment in the box is protected. Then, the adherent cells are separated from the inner wall to form a suspension liquid through centrifugation. The splashing of the culture liquid is avoided by the sealing plate. Then, the culture liquid is stirred at a low speed by a stirring mechanism. Since the adherent cells are separated from the inner wall in advance and move with the culture liquid, the damage to the cells in the stirring process is reduced, and the uniform distribution of the culture liquid is ensured.

The present disclosure is generally related to recombinant microbial cells expressing heterologous proteins of interest. Certain aspects of the disclosure are therefore related to, inter alia, recombinant Bacillus cells having enhanced protein production capabilities, novel protein signal sequences, recombinant polynucleotides encoding heterologous proteins of interest, and related compositions and / or methods thereof. Thus, as exemplified herein, the recombinant Bacillus cells of the instant disclosure are particularly suitable for use in the expression, production and secretion of heterologous proteins.

This invention discloses the application of the NCgl0214 protein and its biomaterials in the production of L-isoleucine, belonging to the field of biotechnology. The technical problem solved by this invention is how to increase the yield of L-isoleucine. The sequence of the protein disclosed in this invention is SEQ ID No. 4 or SEQ ID No. 2. Replacing the coding gene of the protein shown in SEQ ID No. 2 in the starting organism cell with the coding gene of the protein shown in SEQ ID No. 4, or increasing the content or activity of the protein shown in SEQ ID No. 2 or SEQ ID No. 4 in the starting organism cell, can both increase the yield of L-isoleucine.

The present disclosure relates generally to recombinant microbial cells expressing heterologous proteins of interest. Accordingly, certain aspects of the present disclosure relate, inter alia, to recombinant Bacillus cells having enhanced protein production capabilities, novel protein signal sequences, recombinant polynucleotides encoding heterologous proteins of interest, and related compositions and / or methods thereof. Thus, as exemplified herein, the recombinant Bacillus cells of the present disclosure are particularly suitable for use in the expression, production, and secretion of heterologous proteins.

The present invention relates to a receptacle (10) configured to be inserted into a human body and / or an animal body, including a housing (12) and a chamber (14) defined within the housing (12). The chamber (14) is configured to receive a main substance (42) configured to selectively emit carbon monoxide after being activated by an activation substance (44). The chamber (14) is enclosed by a wall (16) of the housing (12) which is permeable to the carbon monoxide emitted by the main substance (42) such that the receptacle (10) can release the carbon monoxide emitted by the main substance (42) to an environment. The receptacle (10) is configured such that the activation substance (44) can be introduced into the chamber (14) from outside of the housing (12), in a state in which the main substance (42) is already arranged within the chamber (14). The present invention further relates to a device (40) for selectively releasing carbon monoxide, a treatment system (60; 80) and a set for treating biological cells, preferably living cells, a method for treating a mammal, a use of carbon monoxide, and method of treating biological cells.

Devices for observing biological activity in a sample, and methods for quantifying tumor infiltration events in a biological sample. The apparatus comprises: a microfluidic device having a plurality of fluid channels, including a central channel adjacent to a medium channel and defining a pourable partition therebetween; wherein the central channel is used for promoting the biological cells contained in the central channel to undergo interstitial transformation (EMT) induced by the reagent supplied to the culture medium channel.

The invention provides novel sample chambers, units and multi-well plates, and systems and methods thereof, for built-in measurement assurance of cell counting methods and calibrated and / or quality-assured measurement and analysis of diverse types of biological cells, e.g., cell count, cell size, cell concentration, cell sub-population, cell morphology, cell viability, etc.