545 results about "Biology cell" patented technology

The invention belongs to the field of microbial culture, and particularly relates to microbial cell culture equipment which comprises an incubator main body, an oscillation platform is arranged in the incubator main body, the front end of the incubator main body is rotatably connected with an incubator door, the rear end of the incubator door is provided with a sliding mechanism, the sliding mechanism comprises two groups of first connecting rods, and the bottom ends of the first connecting rods are rotatably connected with connecting assemblies; a second connecting rod is rotationally connected to the bottom end of the connecting assembly, a pulley is fixedly connected to the side, close to the moving platform, of the second connecting rod, and the pulley is slidably connected to the interior of the sliding groove. Through the structural design of the sliding mechanism, when the incubator door is pulled to open the incubator main body, the first connecting rod, the second connecting rod and the connecting assembly at the rear end of the incubator door can take out the moving platform on the oscillating platform, so that the operation does not need to be performed inside the microorganism culture when the culture container is taken out.

The invention relates to the technical field of classified storage of biological data, in particular to a classified storage method and system for biological cell data. The method comprises the following steps: acquiring multisource cytomics data; performing data standardization on the multisource cytomics data to obtain standard cell characteristic data; performing characteristic matrix conversion on the standard cell characteristic data to obtain a cell characteristic digital matrix; performing molecular fingerprint construction on the biological cells based on the cell characteristic digital matrix to obtain cell molecular characteristic fingerprint data; performing hierarchical classification on the standard cell characteristic data to obtain a cell phenotype classification system; performing hierarchical labeling on the standard cell characteristic data according to the cell phenotype classification system to obtain cell phenotype labeling data; and performing ontology mapping on the cell phenotype labeling data to obtain a cell phenotype relationship network. According to the method, refined classification, dynamic updating and efficient storage of the biological cell data are realized.

The invention relates to the field of biological cells, and particularly discloses a method for quantitatively analyzing metabolites in biological cells based on a Raman spectrum technology, and the method comprises the following steps: S1, collecting a Raman spectrum data set; s2, metabolite with uniform distribution and metabolite with stable content in the cells to be compared are selected as internal standards; s3, extracting the Raman characteristic peak area of the target object, and performing Raman distribution imaging in the cells according to the Raman characteristic peak area of the target object to obtain Raman imaging pictures of a plurality of substances; s4, performing Raman imaging normalization on the target object; s5, converting the normalized Raman peak intensity of the target object into a thermal image, and presenting Raman signals with different intensities in different colors to obtain an intracellular distribution diagram of each component of the intracellular metabolite; and S6, spatial distribution and quantitative analysis of intracellular metabolites. The method disclosed by the invention has the advantages of in-situ, nondestructive, high speed, quick effect, capability of simultaneously and quantitatively analyzing various metabolites on the subcellular scale and the like.

The present invention discloses a recombinant glycosylase base editing system and its application. The recombinant glycosylase base editing system of the present invention is an APOBEC-nCas9-Ung1 or APOBEC(R33A)-nCas9-Rad51-Ung1 base editing system; the base editing system APOBEC-nCas9-Ung1 includes cytosine deaminase APOBEC, nuclease nCas9 and uracil DNA glycosylase Ung1; the base editing system APOBEC(R33A)-nCas9-Rad51-Ung1 includes cytosine deaminase APOBEC(R33A), nuclease nCas9, single-stranded DNA binding protein Rad51 and uracil DNA glycosylase Ung1. Experiments have shown that the recombinant glycosylase base editing system of the present invention can improve the editing efficiency of base C to base G in the genome sequence of an organism or a biological cell and the purity of the editing product.

The present invention relates to nucleic acid modified biological cells and methods of modulating expression of a nucleotide sequence encoding a target in a nucleic acid modified cell.

The present disclosure relates generally to an autonomous cell imaging and modeling platform, and more specifically to machine-learning techniques for using microscopy imaging data to continuously study live biological cells. The autonomous cell imaging and modeling platform can be applied to evaluate various cellular processes, such as cellular differentiation, optimization of cell culture (e.g., in-plate cytometry), disease modeling, histopathology imaging, and genetic and chemical screening, using a dynamic universal imaging system. In some embodiments, the platform comprises a set of label-free computational imaging techniques, self-supervised learning models, and robotic devices configured in an autonomous imaging system to study positional and morphological characteristics in particular cellular substructures of a cell culture in an efficient and non-destructive manner over time.

In one embodiment, a method is disclosed to determine one or more biological cells of interest to identify and count in a mixed biological sample fluid with differing biological cells. The method can include selecting cell markers associated for biological cells to which conjugated antibodies can attach with differing fluorescent dyes; displaying a panel builder graphical user interface window to display a co-expression matrix by biological cell type to assist in selecting cell markers to assign co-expression; and selecting cell markers to assign co-expression with an input device.

The invention provides a Burkholderia paradensis (Paraburkholderia caledonica) and an application of the Burkholderia paradonica (Paraburkholderia caledonica). The strain provided by the invention can realize green and efficient recovery of various rare earth raw materials. According to the present invention, the bacterial strain can produce a variety of organic acids and secondary metabolites having complexing properties during the metabolism process, and the electron transfer process on the surface of the microbial cell membrane provides important effects in the leaching of different rare earth raw materials;

The invention discloses an application of NCgl0762 protein and a mutant thereof in production of L-isoleucine, and belongs to the technical field of biology. The technical problem to be solved by the invention is how to improve the yield of L-isoleucine. The sequence of the protein disclosed by the invention is SEQ ID No. 4 or SEQ ID No. 2. The yield of the L-isoleucine can be increased by replacing the coding gene of the protein as shown in SEQ ID No.2 in starting organism cells with the coding gene of the protein as shown in SEQ ID No.4 or increasing the content or activity of the protein as shown in SEQ ID No.2 or SEQ ID No.4 in the starting organism cells.

The invention discloses a rotary biological culture and observation device, and belongs to the technical field of biological culture devices, the rotary biological culture and observation device comprises a control display terminal and a control host, and further comprises an environment adjusting system, a culture dish rotating system arranged in the environment adjusting system and an imaging system; the environment adjusting system comprises a culture cabin, the culture cabin comprises a cabin chamber and a cabin door connected with the cabin chamber, and a plurality of culture dish rotating systems are circumferentially distributed in the cabin chamber. By adopting the rotary biological culture and observation device, the culture dish rotating system can optimize a cell growth environment and improve the nutrient exchange rate of biological cells by utilizing centrifugal force generated by rotation and hydrodynamic characteristics, and the environment adjusting system can provide a gas development environment and a temperature development environment for biological sample culture; the phenomena of slow development or death of biological sample cells are reduced, and the culture success rate is increased. Meanwhile, the imaging system can realize real-time imaging and observation of the development state of the biological sample on the premise of not destroying the culture environment.

The invention discloses a cell drug screening device and method based on microstructure induced shear acoustic streaming. The micro-structure array processed and manufactured on the acoustic flow driving chip is utilized, so that the high-frequency acoustic surface wave and the micro-structure unit generate acoustic-solid coupling and oscillation, an acoustic streaming effect is generated through excitation in the micro-channel cavity, a stable acoustic flow field is formed, the acoustic flow field generates micro-acoustic streaming shearing, and the acoustic flow effect is improved. The biological cells in the acoustic flow field are subjected to controllable shearing force to change the permeability of cell membranes, so that the medicine is accelerated to enter the cells, and the medicine screening of the cells is promoted; the sizes, the shapes and the periods of the microstructure units are different, the microstructure units respectively and correspondingly excite micro-acoustic streams with different flow speeds, corresponding shearing forces are different, and then different stimulation effects are brought to cell membranes; screening the cell drugs to obtain the specific effect of the drugs on the cells.

The invention relates to the technical field of cell culture equipment, and discloses biological cell culture equipment for biological experiments, which comprises a box body and a frame body, the frame body is mounted in the box body through a bracket, a plurality of partition plates are fixedly connected in the frame body, and the plurality of partition plates divide the frame body into a plurality of culture chambers; a plurality of uniformly distributed placing tables are fixedly connected in the plurality of culture chambers, culture dishes are arranged on the placing tables, two sliding seats are fixedly connected in the box body, a transverse moving mechanism is jointly connected in the two sliding seats, the transverse moving mechanism is provided with a base, a vertical pipe is mounted at the upper end of the base, and a lifting assembly is mounted in the vertical pipe. According to the biological cell culture equipment for the biological test, various cells can be cultured in different areas in different culture environments, a technician can be conveniently assisted to take and place a culture dish during culture, the equipment does not need to be opened to take out the culture dish to observe the culture progress of the cells in the culture process, and the stable culture environment in the equipment is not influenced.

The invention discloses a construction method and application of an iPSC-derived mesenchymal stem cell strain for overexpressing a VEGFA gene based on a CRISPR / Cas9 technology, and relates to the technical field of biological cells, the construction method of the iPSC-derived mesenchymal stem cell strain for overexpressing the VEGFA gene based on the CRISPR / Cas9 technology comprises the following steps: S1, designing sgRNA and ssDNA for an insertion site AAVS1, and detecting the cutting efficiency of the sgRNA; s2, introducing a CRISPR (clustered regularly interspaced short palindromic repeats) system into the cells in an electroporation manner to obtain an iPSC cell strain into which an exogenous gene VEGFA is inserted at a fixed point; s3, carrying out directional induced differentiation on the iPSC cell strain to the iMSC to obtain an iMSC cell strain with overexpression of VEGFA (Vascular Endothelial Growth Factor A) protein; the iMSC of the overexpressed VEGFA protein can well stimulate vascular endothelial growth and promote angiogenesis, and more possibilities are provided for repair treatment of ischemia-related diseases.

Methods are provided for amplifying microbial cell free DNA (mcfDNA) in blood samples. In contrast to amplicon sequencing, in which regions between two conserved sequences are targeted for PCR amplification, the methods herein use a single conserved sequence as the primer annealing site to initiate PCR amplification. Enrichment PCR (E- PCR) is performed using a degenerate primer complementary to a conserved region in a microbial phylogenetic marker gene and an additional primer to the opposite end of the mcfDNA. The conserved region is adjacent to a hypervariable region. Amplification PCR is performed on the enriched mcfDNA fragments using a modified degenerate primer having a 3 '-end extension relative to the degenerate primer, and the additional primer, thereby generating enriched amplified mcfDNA fragments. Sequencing the amplified mcfDNA enables identification of microbial species, which is useful for diagnosing and monitoring inflammatory diseases and cancer, including stratification of cancer by stage, type, and tumor subtype.

The invention discloses a method for automatically collecting nucleic acid of aquatic microorganisms. According to the invention, a water sample is injected into a coagulation reaction bin with a temperature control function, and microbial cell lysis is realized through intermittent low-speed stirring; injecting a surface modification magnetic bead suspension into the reaction bin, starting a multi-shaft stirrer to realize efficient combination of a nucleic acid-magnetic bead compound, and synchronously starting temperature control in the bin to maintain RNA stability; by integrating high-precision positioning, pressure sensing and temperature control cracking core technologies, whole-process standardized operation is realized. The device adopts a dual-mode positioning and pressure sensor to ensure millimeter-level precision of geohydrological data of a sampling point; the three-dimensional turbulent flow field is combined with the magnetic separation array, so that the nucleic acid capture rate is greatly improved, and the risk of nucleic acid degradation is reduced as much as possible. According to the modularized flow path system, through three-stage gradient washing and nanometer ultraviolet sterilization, the problems that in traditional water sample collection, the sample size is large, information is prone to being lost, and treatment lags are effectively solved, and the overall collection efficiency and collection accuracy are improved.

To provide a method for producing vinyl catechol compounds that enables the production of the vinyl catechol compounds without heavy metals.SOLUTION: A method for producing a vinyl catechol compound disclosed herein includes the step for reacting a vinylphenol compound represented by formula (I) with at least one selected from the group consisting of a first microbial cell containing 4-hydroxyphenylacetic acid-3-monooxygenase, a processed product of the first microbial cell, a second microbial cell containing phenol-2-monooxygenase, and a processed product of the second microbial cell. In formula (I), R represents hydrogen or a methyl group, and the position of the hydroxyl group is in the meta or para position relative to the -C(R)=CH2 group.SELECTED DRAWING: None

The utility model relates to the technical field of medical devices, and discloses temperature-adjustable biological cell culture equipment which comprises a main body, a placing plate is clamped in the main body, a container box for storage is clamped at the top of the placing plate, a test tube rack is clamped at the top of the placing plate, and the test tube rack is clamped at the bottom of the main body. Limiting rods used for placing are arranged in the main body, an electric heating plate is fixedly installed in the main body, and a protection plate is connected to one side of the main body in a clamped mode. According to the temperature-adjustable biological cell culture equipment, through cooperative arrangement of the main body, the electric heating plate and the protection plate, when the equipment is used, the internal temperature can be better adjusted, the overall sealing effect is kept, the internal heat loss is reduced, the stable temperature is ensured, and the accuracy in an experiment is improved; and through the cooperative arrangement of a placement plate, a container box, a test tube rack and a limiting rod, the internal space differentiation can be better realized when the device is used.

The invention discloses a filtering and sorting device for biological cell culture and a use method of the filtering and sorting device, and belongs to the technical field of biological cell culture.The filtering and sorting device is provided with a first arc-shaped connecting block, a sealing ring, an arc-shaped connecting clamping plate, a clamping spring, a fixing clamping column, a guide cylinder and a top cover, and the arc-shaped connecting clamping plate is tightly attached to the surface of the sealing ring; meanwhile, a push plate is pushed to drive a fixed clamping column to move, so that a clamping spring is extruded and deformed on the surface of a mounting column, after an arc-shaped connecting clamping plate is tightly attached to the surface of the sealing ring, the fixed clamping column corresponds to clamping grooves in the two sides of an I-shaped clamping block in position, and the push plate is loosened to jack up the fixed clamping column to be clamped into the clamping grooves in the two sides of the I-shaped clamping block under the rebound effect of the clamping spring; and therefore, the concave mounting blocks are clamped and fixed to the two sides of the I-shaped clamping blocks, the arc-shaped connecting clamping plates are tightly attached and fixed to the surface of the sealing ring to connect and fix the collecting barrel and the top cover, and therefore the collecting barrel, the sealing ring and the top cover can be conveniently connected and fixed or detached and cleaned, and the flow guide barrel can be detached and replaced.

The invention discloses a temperature-controllable irradiation experiment device, and belongs to the technical field of irradiation experiment equipment, the temperature-controllable irradiation experiment device comprises a control base, and an irradiation box body is arranged on the upper surface of the control base; a temperature control bearing part used for bearing a plurality of irradiation samples and a driving unit used for adjusting the position and angle of the temperature control bearing part are arranged in the irradiation box body, and an adjusting unit used for adjusting the irradiation dose received by each irradiation sample is arranged on the temperature control bearing part. The irradiation dose of each irradiation sample can be independently and accurately regulated and controlled through the regulating unit, the strict requirements of complex experiments on irradiation dose differentiation of different samples are met, and accurate data support is provided for the fields of material irradiation modification, biological cell irradiation research and the like.

A hyaluronic acid based three dimensional bioengineered vessel includes living biological cells, and norbornene functionalized hyaluronic acid (Nor-HA) comprising a hyaluronic acid derivative and a plurality of norbornene moieties covalently linked to the hyaluronic acid derivative, at least a portion of the plurality of norbornene moieties being crosslinked via a thiol linkage, respectively, to each of a thiol functionalized metalloproteinase (MMP) sensitive peptide, and a thiol functionalized RGD peptide to provide an HA-crosslinked hydrogel matrix that comprises HA- norbornene-MMP sensitive peptide-norbornene-HA linkages and HA-norbornene- cell adhesive peptide-biological cell linkages, the matrix maintained in a culture medium comprising MV2 culture media and at least VEGF-C and Ang2. The three dimensional bioengineered vessel comprises one or more of individual tubes, branched tubes, lymphatic capillaries with button like junction, and lymphatic valve with zipper like junction, and can transport lymph fluid and immune cells.

The invention discloses a rapid biological cell classification imaging method and system based on a quantum correlation diffraction optical neural network, and the method comprises the steps: regulating and controlling a signal photon to an orbital angular momentum state optimized by a neural network based on a time-energy correlation two-photon pair generated in a spontaneous parametric down-conversion process, and irradiating a biological cell sample; a diffractive optical neural network is utilized to construct a quantum state illumination diffractive optical processor, photons carrying cell complex amplitude information are mapped to different detection areas of a predefined image plane, and rapid and high-precision cell classification and recognition are realized through correlation imaging results of an enhanced charge coupled camera. The method has the signal-to-noise ratio and anti-interference capability exceeding the classic limit, can keep high-precision cell classification capability under high background noise, and is suitable for long-time monitoring of living cells under the condition of extremely low light intensity. The method has the characteristics of low energy consumption and high efficiency, and meets the requirements of green calculation and sustainable development. And the method can be used for rapidly classifying and identifying the living biological cells without marks and phototoxicity.

Embodiments of the present invention may provide protecting, preserving, and even repairing biological cells during in vitro processing. A uniform environment such as a protective layer with exogeneous lipids may be formed around biological cells which may interact with the plasma membranes of the cells.

A monitoring device of biological cells including: a fluidic channel in which a fluid including biological cells is made to flow, including a first and second glass walls placed in parallel and a reflective surface adjoined to the second wall; and a piezoelectric transducer, the piezoelectric transducer emitting acoustic waves creating a force acting on the biological cells, making them flow substantially along a single motion plane. The external interface between the first wall and the exterior of the fluidic channel and the reflective surface are configured to function as a Fabry-Perot interferometer, such that the motion planes sensibly perpendicular to the optical axis of the Fabry-Perot interferometer. The finesse of the interferometer is lower than 10. The monitoring device further includes a detecting unit detecting a fringe pattern stemming from the Fabry-Perot interferometer and an analysing device, analysing the fringe pattern and configured to output data on physical properties of the cells based on the pattern.

A recombinant microbial cell is capable of producing at least one compound having structural Formula II from a carbon source:In Formula II, n=2-3; R1=H or COCH3 or CH2CH2COX with X=OH or O—; R2=CH3 or CH2CH2COX with X=OH or O— where the cell comprises a genetic modification to increase activity relative to its wild-type cell of E4 where E4 is a desferrioxamine or bisucaberin synthetase (EC 6.3.-.-) (E4i) capable of converting N5-aminopentyl-N-(hydroxy)-succinamic acid to desferrioxamine B or H or at least one other linear desferrioxamine or bisucaberin according to Formula II.

The present application relates to the technical field of biological cell culture, and particularly relates to a three-dimensional cell culture device with automatic liquid replacement function, comprising: a box body; a rotating assembly, a liquid replacement assembly and a control display assembly are integrally installed in the box body. The three-dimensional cell culture device with automatic liquid replacement function provided by the present application integrates microgravity simulation and liquid replacement function in a relatively closed culture box body space, ensures that the culture environment in the cell culture process has high stability by not excessively affecting the air flow, temperature and humidity environment, etc. in the culture box body during liquid replacement; the device for replacing culture solution does not need to rotate, effectively releases the culture space while reducing the energy consumption required in the rotation process, can load multiple culture bottles for three-dimensional culture and automatic liquid replacement at the same time, and has high potential for large-scale cell preparation.

It can be useful to regulate the growth of microbial cells. Some embodiments herein provide genetically engineered microbial cells that can produce bacteriocins to control the growth of microbial cells. In some embodiments, microbial cells are contained within a desired environment. In some embodiments, contaminating microbial cells are neutralized. In some embodiments, a first microbial cell type regulates the growth of a second microbial cell type so as to maintain a desired ratio of the two cell types.

The invention relates to a method (500) and a device (100) for unmasking biological cells, in particular circulating tumor cells, by means of plasmin, wherein a reactant (10) is added to a sample containing plasminogen and biological cells, in particular a blood sample, in order to convert plasminogen to plasmin.

Disclosed are genetically engineered microbial cells for the production of oligosaccharides comprising a galactose-β1,4-glucose moiety at their reducing end, wherein said microbial cells are able to produce said oligosaccharides in the absence of exogenously added lactose, and a method of producing said oligosaccharides using said microbial cells.

The invention provides a biological cell freezing visualization system and method based on a metal woven belt, and relates to the technical field of biologication.The biological cell freezing visualization system comprises a bottom plate, a support platform, a profound hypothermia liquid tank and an observation assembly are fixedly connected to the upper surface of the bottom plate, a lifting sliding table is fixedly connected to the inner bottom face of the support platform, and a constant hypothermia copper table is fixedly connected to the upper end of the lifting sliding table; the constant hypothermia copper table is communicated with the deep hypothermia liquid tank through a liquid conveying hose, the inner top surface of the support platform is fixedly connected with a woven belt fixing support and a sample placing platform, one end of a metal woven belt is fixedly connected to the lower surface of the woven belt fixing support, and the other end of the metal woven belt is fixedly connected to the lower surface of the sample placing platform; a cover plate covers the sample placing platform, and the observation assembly is used for shooting an image of the sample placing platform. Cold is conducted through the metal woven belt, the requirement for the movement precision of the device is low, the durability of the device is improved, the position of the sample containing platform is fixed, and it is guaranteed that imaging of the observation assembly is clear.