Preparation method for biomimetic matrix type biological wound healing material

Abstract

The invention provides a preparation method for a novel biomimetic matrix type biological wound healing material. According to the method, small intestinal submucosa with a three-dimensional scaffold structure is compounded with platelet-rich blood plasma containing a specific proportion of bioactive factors, and a specific process is adopted to carry out modification to form the novel material containing biological induction factors, wherein the novel material has a three-dimensional biomimetic structure. With the biomimetic matrix type biological wound healing material prepared by the method of the present invention, the healing can be promoted, the physiological repair can be promoted, the pathological repair can be inhibited, and the regeneration repair efficiency and the repair quality of the damaged tissue can be improved. The novel biological wound healing material of the present invention can be used for repairs of various human soft tissues, membranous tissues or cavity walls, and for cavity filling, and can be used as the ideal extracellular matrix biomimetic material and the cell scaffold material for wound repairs of the tissues and the organs, promotion of wound healing, prevention of adhesion and the like.

Description

technical field

[0001] The invention relates to a method for preparing a medical bionic material, in particular to a method for preparing a composite and biomimetic biomimetic matrix type biological healing material through a specific crosslinking method. Background technique

[0002] The regeneration and repair of tissue defects caused by factors such as trauma, infection, tumor, functional atrophy, and surgery has always been one of the main problems in clinical treatment. Solving the above-mentioned problems through alternative tissue repair with biological healing materials is a current research hotspot, and it is also the focus of general attention of scholars at home and abroad. The structure and performance of bio-healing materials currently available clinically are significantly different from those of body tissues. After implantation, they stimulate each other with the body to produce various irritating reactions, which seriously affects the quality of clinical trea...

Examples

Embodiment 1

[0031] The steps of the preparation method of the bionic matrix type biological healing material of the present invention are as follows:

[0032] Step 1: Select porcine small intestines with traceable sources that meet inspection and quarantine requirements, scrape off redundant tissues such as muscle and serosa, rinse with deionized water, and soak in EDTA (100mmol / L) / NaOH (10mmol / L) solution for 16 hours , rinsed with deionized water and placed in hydrochloric acid / PBS solution with a pH value of 3~4 for 6~8h, rinsed with deionized water to remove residual acid solution, soaked in phosphate buffer saline (PBS) for 16~18h, repeated in deionized water rinse. Soak in 0.1% peracetic acid / 20% ethanol solution for 8 hours, then wash in 0.05% sodium azide / PBS solution for 2 hours, and sterilize by γ-ray irradiation. Homogenize aseptically to prepare SIS hydrated gel.

[0033] Step 2: Prepare platelet-rich plasma (PRP) by secondary centrifugation: select a 50ml sterile needle tub...

Embodiment 2

[0040] The steps of the preparation method of the bionic matrix type biological healing material of the present invention are as follows:

[0041] The first step: with embodiment 1.

[0042] Second step: with embodiment 1.

[0043] The third step. Take the prepared SIS hydration gel 0.5g and PRP 0.25g, stir and mix.

[0044] Step 4: Add 0.2% Genipin / NaHCO to the mixture in Step 3 by aseptic operation at 4°C 3 Solution (pH value 10) 0.75ml, mix well, let stand for 1.5h, add 10% CaCl 2 Solution 3.5ml, mix well. Cross-linking at room temperature for 5.5h.

[0045] Step 5: Discard the residual solution, and rinse repeatedly with 10ml of sterile PBS solution several times.

[0046] Step 6: Put the above reaction product into a sterile mold, freeze and thaw repeatedly at -20°C for 3 times, and then freeze-dry and shape it to obtain a biomimetic matrix biocure material.

Embodiment 3

[0048] The steps of the preparation method of the bionic matrix type biological healing material of the present invention are as follows:

[0049] The first step: with embodiment 1.

[0050] Second step: with embodiment 1.

[0051] Step 3: Take the prepared SIS hydration gel 0.5g and PRP 0.5g, stir and mix.

[0052] Step 4: Add 0.25% alginate / acetic acid solution (pH 5) 0.5ml to the mixture in step 3 under aseptic operation at 4°C, mix well, let stand for 1 hour, add 10 %CaCl 2 Solution 4.5ml, mix well. Crosslink at room temperature for 5 h.

[0053] Step 5: Discard the residual solution, and rinse with 12.5ml sterile PBS several times in small quantities.

[0054] Step 6: Put the above-mentioned reaction product into a sterile mold, freeze and thaw repeatedly at -20°C for 3 times, and then freeze-dry and shape it to obtain a biomimetic matrix biocure material.

technical field

[0001] The invention relates to a method for preparing a medical bionic material, in particular to a method for preparing a composite and biomimetic biomimetic matrix type biological healing material through a specific crosslinking method. Background technique

[0002] The regeneration and repair of tissue defects caused by factors such as trauma, infection, tumor, functional atrophy, and surgery has always been one of the main problems in clinical treatment. Solving the above-mentioned problems through alternative tissue repair with biological healing materials is a current research hotspot, and it is also the focus of general attention of scholars at home and abroad. The structure and performance of bio-healing materials currently available clinically are significantly different from those of body tissues. After implantation, they stimulate each other with the body to produce various irritating reactions, which seriously affects the quality of clinical trea...

Examples

Embodiment 1

[0031] The steps of the preparation method of the bionic matrix type biological healing material of the present invention are as follows:

[0032] Step 1: Select porcine small intestines with traceable sources that meet inspection and quarantine requirements, scrape off redundant tissues such as muscle and serosa, rinse with deionized water, and soak in EDTA (100mmol / L) / NaOH (10mmol / L) solution for 16 hours , rinsed with deionized water and placed in hydrochloric acid / PBS solution with a pH value of 3~4 for 6~8h, rinsed with deionized water to remove residual acid solution, soaked in phosphate buffer saline (PBS) for 16~18h, repeated in deionized water rinse. Soak in 0.1% peracetic acid / 20% ethanol solution for 8 hours, then wash in 0.05% sodium azide / PBS solution for 2 hours, and sterilize by γ-ray irradiation. Homogenize aseptically to prepare SIS hydrated gel.

[0033] Step 2: Prepare platelet-rich plasma (PRP) by secondary centrifugation: select a 50ml sterile needle tub...

Embodiment 2

[0040] The steps of the preparation method of the bionic matrix type biological healing material of the present invention are as follows:

[0041] The first step: with embodiment 1.

[0042] Second step: with embodiment 1.

[0043] The third step. Take the prepared SIS hydration gel 0.5g and PRP 0.25g, stir and mix.

[0044] Step 4: Add 0.2% Genipin / NaHCO to the mixture in Step 3 by aseptic operation at 4°C 3 Solution (pH value 10) 0.75ml, mix well, let stand for 1.5h, add 10% CaCl 2 Solution 3.5ml, mix well. Cross-linking at room temperature for 5.5h.

[0045] Step 5: Discard the residual solution, and rinse repeatedly with 10ml of sterile PBS solution several times.

[0046] Step 6: Put the above reaction product into a sterile mold, freeze and thaw repeatedly at -20°C for 3 times, and then freeze-dry and shape it to obtain a biomimetic matrix biocure material.

Embodiment 3

[0048] The steps of the preparation method of the bionic matrix type biological healing material of the present invention are as follows:

[0049] The first step: with embodiment 1.

[0050] Second step: with embodiment 1.

[0051] Step 3: Take the prepared SIS hydration gel 0.5g and PRP 0.5g, stir and mix.

[0052] Step 4: Add 0.25% alginate / acetic acid solution (pH 5) 0.5ml to the mixture in step 3 under aseptic operation at 4°C, mix well, let stand for 1 hour, add 10 %CaCl 2 Solution 4.5ml, mix well. Crosslink at room temperature for 5 h.

[0053] Step 5: Discard the residual solution, and rinse with 12.5ml sterile PBS several times in small quantities.

[0054] Step 6: Put the above-mentioned reaction product into a sterile mold, freeze and thaw repeatedly at -20°C for 3 times, and then freeze-dry and shape it to obtain a biomimetic matrix biocure material.

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