15 results about "Small intestinal submucosa" patented technology

The invention relates to the field of new biological materials, in particular to a high-packaging-dose antibacterial slow-release biological patch obtained by packaging at least two antibiotics with an acellular matrix material. The acellular matrix material is derived from any two of small intestinal submucosa, peritoneum, dermis, bladder basement membrane and pericardium; the antibiotics comprise hydrophobic antibiotics and hydrophilic antibiotics, and the hydrophobic antibiotics are selected from one or more of Beta-lactam antibiotics, rifamycin and macrolide antibiotics; the hydrophilic antibiotics are tetracycline antibiotics. The coating-free drug-loaded sustained-release system based on a lamination process kills bacteria in a short period, prevents colonization and reproduction of bacteria in a wound / patch, realizes synergistic and continuous release of antibiotics along with a wound healing process, guides tissue regeneration and synchronously realizes degradation, is applied to a high-incidence infection period of a surgical operation, reduces adhesion, and has a good application prospect. The abdominal wall strength is enhanced, and the growth of wound granulation tissues is promoted.

The invention discloses an implantable acellular matrix material and a preparation method thereof, and belongs to the field of biomedical materials. According to the method, natural animal tissues (such as pericardium, achilles tendon or small intestine submucosa) are used as raw materials, a low-temperature chemical combined decellularization treatment technology is adopted, and the method comprises the multi-step synergistic process of pre-cooling soaking balancing, low-temperature enzymolysis, low-concentration surfactant gradient permeation cleaning, low-temperature nuclease degradation, non-crosslinking sterilization and the like. Under the condition that the treatment temperature is strictly kept at 0-8 DEG C, cell components and genetic materials (the residual DNA content is 1t, and the dry weight is 8 ng / mg) are efficiently removed, and meanwhile the natural three-dimensional fine structure of an extracellular matrix (ECM), the integrity of collagen fibrils and key bioactive components (such as glycosaminoglycans (GAGs)) are reserved to the maximum extent. The host immunological rejection reaction of the obtained material is remarkably reduced, host cell ingrowth, vascularization and tissue function reconstruction can be effectively promoted after implantation, and the material is suitable for high-end implantation scenes such as soft tissue repair and regenerative medical stents.

The application provides a platelet-responsive self-gelation hemostatic material and a preparation method and application thereof, and belongs to the medical field. Based on a multiple cross-linking strategy of dynamic covalent bonds and non-covalent interactions, a hemostatic material capable of realizing self-gelation quickly is designed by using modified sodium alginate and hydroxylated porcine small intestinal submucosa as raw materials. The hemostatic material has good hydrophilicity and liquid absorption capacity, can realize powder-gelation transformation in a short time, form a stable physical barrier, has platelet response characteristics, can promote platelet adhesion and activation, accelerate the blood coagulation process of the body, and improve the mechanical properties and tissue adhesion of the material, and has a good application prospect.

The application discloses an animal small intestinal submucosa acellular matrix anti-adhesion membrane and a preparation method and application thereof, and belongs to the field of biological materials. The animal small intestinal submucosa acellular matrix anti-adhesion membrane is obtained by sterilizing one side of the animal small intestinal submucosa acellular matrix after laser treatment. The animal small intestinal submucosa acellular matrix anti-adhesion membrane has reduced thickness, improved degradation performance, can guide postoperative wound tissue regeneration, can prevent other tissues from adhering to a wound surface, and is particularly suitable for a case that the degradation rate of a sheet material needs to be further improved, and has a wide application prospect in preparation of anti-adhesion tissue repair materials.

The invention belongs to the technical field of biological medicine, and particularly relates to cellulose cross-linked porcine small intestinal submucosa hydrogel as well as a preparation method and application thereof. The cellulose cross-linked pig small intestine submucosa hydrogel comprises a pig small intestine submucosa hydrogel and dialdehyde cellulose cross-linked with the pig small intestine submucosa hydrogel, the crosslinking is that the primary amino group of the porcine small intestinal submucosa hydrogel and the aldehyde group of the dialdehyde cellulose form an imine bond. The cellulose cross-linked porcine small intestinal submucosa hydrogel has excellent mechanical properties and biocompatibility, and can be better used for promoting stem cell osteogenic differentiation and preparing refined organoid structures.

The invention provides composite hydrogel and a preparation method and application thereof, and belongs to the technical field of biomedical materials, the composite hydrogel provided by the invention comprises melatonin carbon dots and porcine small intestinal submucosa hydrogel, the melatonin carbon dots are dissolved in the porcine small intestinal submucosa hydrogel, the particle size of the melatonin carbon dots is 1.5-2.5 nm, and the melatonin carbon dots are dissolved in the porcine small intestinal submucosa hydrogel. The loading capacity of the melatonin carbon dots in the composite hydrogel is 150 to 450 [mu] g / mL. The composite hydrogel provided by the invention can effectively inhibit the inflammatory response of achilles tendinopathy, can promote tendon regeneration, and has the advantages of minimally invasive effect, high drug curative effect and small side effect when being used for treating achilles tendinopathy.

The invention relates to a composite hydrogel material based on acellular matrixes from three different sources. According to the material, a fat decellularized matrix (DAT), a small intestine submucosa decellularized matrix (SIS) and a blood vessel decellularized matrix (Adv) are mixed in proportion, and a gel system with the temperature response characteristic is formed through a biocompatible cross-linking agent. The ternary composite acellular extracellular matrix hydrogel disclosed by the invention can keep activity under various storage conditions, so that a wider possibility is provided for cold chain preservation of the product.

The invention belongs to the technical field of medicines, and particularly relates to an sECM-SIS biological membrane as well as a preparation method and application thereof. According to the present invention, an extracellular matrix (sECM) derived from stem cells is integrated to a small intestine submucosa (SIS) to prepare the sECM-SIS biological membrane; after the sECM-SIS biological membrane is implanted into a full-thickness skin defect, wound healing is remarkably accelerated, and the sECM-SIS biological membrane is characterized by rapid wound reepithelization, strong angiogenesis, approximate normal epidermis thickness recovery, mature collagen deposition, skin appendage regeneration and macrophage polarization adjustment. According to the results, the sECM-SIS has huge potential in the aspect of skin wound repair.

The invention discloses multifunctional rapid shape memory composite frozen gel as well as a preparation method and application thereof, and belongs to the technical field of medical material preparation. According to the invention, pig small intestine submucosa (SIS) and polyurethane are subjected to composite crosslinking and chemical modification to prepare the quick shape memory composite frozen gel, and the quick shape memory composite frozen gel has the following advantages: 1) self infiltration: the quick shape memory composite frozen gel has good hydrophilicity, and can quickly absorb liquid which is 40 times of the mass of the quick shape memory composite frozen gel in a dry state; (2) self-expansion is achieved, the shape memory ability is prominent, volume recovery can be rapidly achieved within 1.8 s, and the bleeding part is effectively blocked; 3) self-enhancement: the composition has procoagulant activity, significantly promotes platelet adhesion and activation, and accelerates the coagulation process of the body; and 4) self-degradation: the frozen gel does not need to be taken out for the second time after hemostasis, can be degraded in vivo, and promotes in-situ tissue repair after hemostasis. The method has a wide application prospect in the fields of operations, wound first aid and the like.

The application discloses a multifunctional biomaterial for posterior sclera reinforcement, which is prepared by using layer-by-layer self-assembly (LbL) surface functionalization technology to coat silk fibroin (SF) on the surface of small intestinal submucosa (SIS), and preparing a light cross-linked SF (SFMA) microgroove coating with anisotropic characteristics on one side of the SIS for guiding cell arrangement and growth, promoting tissue regeneration and repair; and preparing a light cross-linked SF and sodium hyaluronate (SF-SH) mixed coating on the other side for improving biocompatibility and adhesion, so as to realize preparation of a multifunctional posterior sclera reinforcement biomaterial with controllable mechanical and degradation properties and capable of promoting surrounding tissue regeneration. The posterior sclera reinforcement biomaterial is cross-linked in the LbL mode to avoid toxic substance residues generated by cross-linking of SIS and traditional glutaraldehyde. The light cross-linked SFMA microgroove coating on one side can not only realize directional growth of fibroblasts, but also realize directional arrangement of collagen type I secreted by the cells, and effectively realize tissue integration. Meanwhile, the light cross-linked SF-SH mixed coating on the other side has excellent adhesion, which is helpful for positioning and attaching of the reinforcement material, and is of great significance to improving the effect of a posterior sclera reinforcement (PSR) operation and promoting long-term stable and effective treatment of pathological high myopia by using the biomaterial.

The application provides a polyurethane / small intestinal submucosa composite material and a preparation method and application thereof, and belongs to the field of medicines. The polyurethane / small intestinal submucosa composite material with an asymmetric structure is prepared by using a freeze-drying and impregnation drying method and different structural forms of the same components. The composite material is porous sponge on one side and dense film on the other side, can effectively prevent leakage on the basis of guaranteeing mechanical properties, and performs full-layer repair. The composite material can adjust the component ratio according to different requirements, and then adjust the mechanical properties, so as to match the normal bladder mechanical behavior of different species. The preparation method of the composite material is simple and convenient, has high popularization, can be popularized to other polyurethane / extracellular matrix materials, and has a good application prospect.

The invention discloses a preparation method of a biodegradable and absorbable hemostatic composition, and belongs to the technical field of medical materials. The technical problems to be solved are that in the preparation process of an existing hemostatic material containing polysaccharide components, a cross-linking agent is used, so that the safety is poor, and the water absorption expansibility is reduced due to poor uniformity of raw materials in the preparation process. According to the technical scheme, the method is characterized by comprising the following steps: firstly, dissolving water-soluble polysaccharide in water to form a water-soluble polysaccharide solution; fully mixing the decellularized small intestinal submucosa particles with sodium carboxymethyl starch, spraying a water-soluble polysaccharide solution, and carrying out wet granulation to obtain wet particles; and carrying out size stabilization, drying and sterilization on the wet particles to obtain the hemostatic composition. Wherein in wet granulation, the pressure of a spray gun is set to be 0.2-0.3 MPa.

A multifunctional biological plugging pad for a liver section belongs to the field of medical instruments. Aiming at the problems of section bleeding, bile leakage and tissue healing after the hepatic resection, the invention provides the multi-layer composite biological plugging pad. The upper layer is an oxidized regenerated cellulose hemostatic layer with the thickness of 1-2mm; the middle layer is a cross-linked polyacrylate and chitosan composite expansion sealing layer and expands when meeting body fluid to form physical plugging; the lower layer is a decellularized small intestinal submucosa regeneration promoting layer which can promote tissue regeneration; and the bottom surface is coated with an NHS activated polyethylene glycol adhesive layer with the thickness of 10-50 microns. The diameter of the product is 3-15cm, the total thickness is 2-5mm, and the material can be degraded and absorbed in vivo. The device is suitable for the hepatobiliary surgery, the pancreatic surgery and other parenchyma visceral organ resection surgery, operation can be simplified, complications are reduced, and postoperative rehabilitation is promoted.

The application adopts small intestinal submucosa SIS and animal membrane tissue AMT as raw materials, respectively carries out deantigen treatment on the small intestinal submucosa and the animal membrane tissue, and obtains a decellularized membrane. The SIS decellularized membrane is subjected to acidolysis and salt precipitation treatment to obtain a gelatin solution. The gelatin solution is subjected to micro-crosslinking under an EDC / NHS crosslinking system, and then the crosslinking agent is removed through dialysis. An antibiotic drug, PAMAM and glycerol are mixed with the micro-crosslinked gelatin solution, PAMAM is used as a drug loading medium to combine with the micro-crosslinked gelatin solution to form a drug loading solution. The SIS decellularized membrane is soaked in the drug loading solution, and then is combined with the AMT decellularized membrane to form a sandwich structure. The drug loading oral membrane has good barrier effect, is safe and effective, can release the antibiotic drug in the degradation process, has a stable slow-release process and length, thereby reducing the pain of patients, improving the success rate of operation and accelerating wound healing.

The invention discloses a multifunctional biological material for posterior sclera reinforcement, which is characterized in that silk fibroin (SF) is coated on the surface of small intestinal submucosa (SIS) by using a layer-by-layer self-assembly (LbL) surface functionalization technology, and a photo-crosslinked SF (SFMA) microgroove coating with anisotropic characteristics is prepared on one side of the SIS and is used for guiding cell arrangement and growth and promoting tissue regeneration and repair; and a photo-crosslinked SF and sodium hyaluronate (SF-SH) mixed coating is prepared on the other side, so that the biocompatibility and the adhesion are improved, and the preparation of the multifunctional posterior sclera reinforcing biological material which has controllable mechanical and degradation properties and can promote the regeneration of surrounding tissues is realized. According to the posterior sclera reinforcing biological material, in order to avoid toxic substance residues generated by cross-linking of SIS and traditional glutaraldehyde, cross-linking is achieved in an LbL mode. One side of the photo-crosslinking SFMA microgroove coating not only can realize directional growth of fibroblasts, but also can realize directional arrangement of I-type collagen secreted by cells, so that effective tissue integration is realized. Meanwhile, the excellent adhesion of one side of the photo-crosslinking SF-SH mixed coating is beneficial to positioning and attaching of the reinforcing material, and the photo-crosslinking SF-SH mixed coating has important significance in improving the posterior sclera reinforcing (PSR) operation effect and promoting the biological material to stably and effectively treat pathological high myopia for a long time.