Temperature-sensitive composite as well as preparation method and application thereof

A composite material, temperature-sensitive technology, applied in the direction of prosthesis, medical science, etc., can solve the problems of loss and degradation, too fast, etc., to achieve the effect of prolonged in vivo degradation, low immunogenicity, and suitable biodegradability

Active Publication Date: 2014-02-26
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems that simple chitosan gel has no bioactive factors and the micropowder of small intestinal submucosa is easy to lose and degrade too quickly after being implanted in the body, the present invention provides a new temperature-sensitive composite material matrix composition and its preparation method and use

Method used

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  • Temperature-sensitive composite as well as preparation method and application thereof
  • Temperature-sensitive composite as well as preparation method and application thereof
  • Temperature-sensitive composite as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Example 1 Preparation method of temperature-sensitive composite material of the present invention

[0077] 1. Preparation of SIS powder

[0078] Take fresh pig small intestine within 4 hours of slaughter, wash it with clean water, cut it into 10 cm small sections, scrape off the serosa layer, mucosa layer and muscular layer of jejunum with blunt instruments, and then wash the remaining submucosa layer repeatedly with normal saline until the water is gone. Turbidity, then degrease with methanol: chloroform (1:1, V / V) mixture for 12 hours, rinse with deionized water until there is no special smell, then soak in 0.05% trypsin at 4°C overnight, rinse with normal saline, and then 0.5% trypsin SDS physiological saline solution was shaken and soaked for 4 hours, rinsed with deionized water, and finally treated with a mixed solution of 0.1% peracetic acid and 20% ethanol for 30 minutes, then rinsed with normal saline. The obtained porcine small intestinal submucosa acellular m...

Embodiment 2

[0081] Example 2 The preparation method of the temperature-sensitive composite material of the present invention

[0082] 1. Preparation of SIS powder

[0083] Take fresh pig small intestine within 4 hours of slaughter, wash it with clean water, cut it into 10 cm small sections, scrape off the serosa layer, mucosa layer and muscular layer of jejunum with blunt instruments, and then wash the remaining submucosa layer repeatedly with normal saline until the water is gone. Turbidity, then degrease with methanol: chloroform (1:1, V / V) mixture for 12 hours, rinse with deionized water until there is no special smell, then soak in 0.05% trypsin at 4°C overnight, rinse with normal saline, and then 0.5% trypsin SDS physiological saline solution was shaken and soaked for 4 hours, rinsed with deionized water, and finally treated with a mixed solution of 0.1% peracetic acid and 20% ethanol for 30 minutes, then rinsed with normal saline. The obtained porcine small intestinal submucosa ace...

Embodiment 3

[0086] Example 3 The preparation method of the composite temperature-sensitive material of the present invention

[0087] 1. Preparation of SIS powder

[0088] Take fresh pig small intestine within 4 hours of slaughter, wash it with clean water, cut it into 10 cm small sections, scrape off the serosa layer, mucosa layer and muscular layer of jejunum with blunt instruments, and then wash the remaining submucosa layer repeatedly with normal saline until the water is gone. Turbidity, then degrease with methanol: chloroform (1:1, V / V) mixture for 12 hours, rinse with deionized water until there is no special smell, then soak in 0.05% trypsin at 4°C overnight, rinse with normal saline, and then 0.5% trypsin SDS physiological saline solution was shaken and soaked for 4 hours, rinsed with deionized water, and finally treated with a mixed solution of 0.1% peracetic acid and 20% ethanol for 30 minutes, then rinsed with normal saline. The obtained porcine small intestinal submucosa ace...

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Abstract

The invention discloses a temperature-sensitive composite matrix compound which comprises the following components in parts by weight: 80-200 parts of chlorinated chitosan, 200-260 parts of beta-disodium glycerophosphate, 37.5-87.5 parts of hydroxyethyl cellulose and 125-375 parts of small intestinal submucosa micropowder. The invention further discloses a temperature-sensitive composite material as well as a preparation method and application thereof. The temperature-sensitive composite has a favorable temperature-sensitive property and intrinsic bioactivity, can sustainably release a plurality of growth factors and has favorable biocompatibility and a good clinical application prospect.

Description

technical field [0001] The invention relates to a temperature-sensitive composite material and its preparation method and application. Background technique [0002] Hydrogel is a gel with water as the dispersion medium. It has a water content close to normal tissue and good biocompatibility. Its physical and chemical properties are controllable and it has a porous structure. Its three-dimensional porous structure is very similar to the extracellular matrix. , is conducive to cell growth, proliferation and differentiation, and is also conducive to the maintenance of the activity of biologically active factors. It can be minimally invasively implanted and has broad application prospects in tissue engineering. [0003] Thermosensitive hydrogel can change from liquid state to gel state within the body temperature range, without other catalysts and severe reaction conditions, can repair various irregular-shaped tissue defects, and can evenly distribute cells or active substances ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/20A61L27/12A61L27/52A61L27/50A61L27/24A61L27/22A61L27/54
Inventor 罗静聪邓学勤秦廷武
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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