The invention discloses a neiyi enema and use and a preparation method thereof. The neiyi enema comprises the following components: 14 to 41 weight parts of Chinese honeylocust spine, 7 to 22 weight parts of zedoary root, 10 to 49 weight parts of corydalis, 12 to 45 weight parts of sargentodoxa, 3 to 18 weight parts of largetrifoliolious bugbane rhizome, and 12 to 31 weight parts of garden balsamstem. Compared with the prior art, the neiyi enema with the structure has the advantages that: by utilizing the characteristic that intestine submucosa veins are plentiful and have vigorous blood flow, the medicine is directly guided to an illness station and easily absorbed, enhances treatment effect and does not have any side effect; meanwhile, the stimulation of the enema to the intestinal canal can be reduced, and the medicine can be retained in the intestinal canal longer to fully exert the medicinal effect, so that the medicine is easier to absorb and quick in response, and can promote the recovery of the patient, shorten the course of treatment and improve local symptoms; in addition, the enema mainly aims at endometriosis, has antiphlogistic and analgesic effects, also can be usedfor patients suffering from pelvic infection bellyache and salpingemphraxis sterility, and can relieve the inflammation of ovarian ducts and improve pelvic environment to make the patients easily impregnated.