48 results about "Intestinal submucosa" patented technology

The invention relates to a method for preparing a biomaterial of acellular small intestinal submucosa, which comprises the steps of preposition treatment, acellular treatment, enzyme treatment, preparations of membranous products and particle products. Compared with the prior art, the biomaterial has higher bioactivity and biocompatibility, no obvious immune rejection and no toxic effect on cells; besides, the biomaterial has a certain mechanical strength and toughness, and variable shape, size and thickness, thereby being convenient for clinical suturing and fixing. At the same time, the preparation method has the advantages of unlimited raw material source, cell-free residues, no ethical issues and being capable of effectively inactivating virus. The prepared products are applicable to the biomedical engineering fields, such as repairing defections of body tissue, serving as tissue filling materials, repairing facial depression deformity, serving as tissue reinforcements to replace fascia, repairing membranous defections and malnourished and infected surfaces of wound, serving as materials for biodegradable stents, and serving as injectable filling materials.

The invention provides a tissue patch and a preparation method thereof. The tissue patch uses an acellular small intestinal submucosa as an inner layer, the two sides of the tissue patch are covered with acellular amniotic membranes; the prepared tissue patch isolates from immunotoxicity of the acellular small intestinal submucosa, and the acellular small intestinal submucosa compensates for the deficiencies of a mechanical strength of the acellular amniotic membrane. Compared with the prior art, the tissue patch has higher biological activity and biocompatibility, no obvious immune rejection and no toxic effect on cells; and the preparation method has the advantages of unlimited raw materials source, cell-free residues, no ethical issues and capability of effectively inactivating virus. The prepared product is applicable to serving as a tissue reinforcement to repair a shortcoming or a defect of a strength of a body soft tissue; the prepared product is used as a tissue filling material and is applicable to the repair of facial depression deformity.

The present disclosure provides a regenerative peripheral nerve interface (RPNI) for a subject comprising an insulating substrate, at least one metallic electrode deposited onto the insulating substrate forming a thin-film array; a portion of the at least one metallic electrode surface having a layer of a first conductive polymer and a layer of decellularized small intestinal submucosa (SIS) coating a portion of the electrode, wherein a second conductive polymer is electrochemically polymerized through the SIS to form the regenerative peripheral nerve interface. The present disclosure also provides that a layer of muscle tissue contacts the regenerative peripheral nerve interface.

The invention provides a mold for preparing an accellular small intestine submucosa matrix material, which is composed of a base and a pressure screen, wherein the base is a square plate with pins on four sides, and the pins are perpendicular to the square plate; the pressure screen is a square frame with a square frame structure or reticular structure inside; and the length of the four sides of the pressure screen is 1-3 cm smaller than that of the base. When being used for preparing the accellular porcine small intestine submucosa matrix material, the mold can not compress the space structure of the accellular porcine small intestine submucosa matrix material or destroy the three-dimensional structure of the natural extracellular matrix, and can ensure the porosity of the prepared accellular porcine small intestine submucosa matrix material.

A bioremodelable prosthesis for treating a patient with a diseased or damaged organs comprising a first layer that contains acid-extracted fibrillar or non-fibrillar collagen, and a second layer derived from the tunica submucosa of the small intestine that provides structural stability, is pliable and is semi-permeable,pe1 59564443.npc wherein the prosthesis undergoes controlled biodegradation occurring with adequate living cell replacement such that the original prosthesis is replaced by the patient's living cells.

The invention discloses a composite biological patch based on pig small intestinal submucosa and a preparation method and application thereof. The composite biological patch is characterized in that the accellular pig small intestinal submucosa is compounded with an additive so as to obtain the composite biological patch based on the pig small intestinal submucos; the additive is selected from one or two of growth factors and medicines. The composite biological patch based on the pig small intestinal submucos has the advantages that the composite biological patch is a good biological stent material, the structure component and the space three-dimensional structure are very similar, the good biological compatibility is presented in the clinical experiment, the healing of wounds is promoted, and various tissues are regenerated and remolded; the biological stent material has effective restoration effect on the healing of skin wounds, and has important meaning on the regeneration of the skins.

The invention provides a mold for preparing an accellular small intestine submucosa matrix material, which is composed of a base, a pressure frame and a pressure screen, wherein the base is a square plate with bolt holes at four corners; the pressure frame is a hollow square frame with bolt holes at four corners; the pressure screen is a square frame with a square frame structure or reticular structure inside; the bolt holes in the base are corresponding to the bolt holes in the pressure frame one by one; the external diameter of the pressure frame is the same as the dimension of the base; and the inner diameter of the pressure frame is the same as the dimension of the pressure screen. When being used for preparing the accellular porcine small intestine submucosa matrix material, the mold can not compress the space structure of the accellular porcine small intestine submucosa matrix material or destroy the three-dimensional structure of the natural extracellular matrix, and can ensure the porosity of the prepared accellular porcine small intestine submucosa matrix material.

The invention relates to a tissue repairing material with bioactivity and a preparation method thereof. The material is made by compounding human body living cells, and extracellular matrixes and cell growth factors synthesized and excreted by the human body living cells on the acellular small intestine submucosa. Natural antigen components are removed from the prepared tissue repairing material with bioactivity, and when the tissue repairing material is applied to a wound, the tissue repairing material can survive and directly take part in the repair of the wound to continuously synthesize and excrete growth factors, guide the cells around the wound to grow in and the creation of blood vessel, and induce the differentiation of stem cells to skin cells, thereby obviously promoting the wound healing; besides, the tissue repairing material not only has part of the characteristics of human body tissues and the good mechanical property of the acellular small intestine submucosa, but also has high biocompatibility to human body, obviously reduces the immune rejection; at the same time, the prepared tissue repairing material can cover the surface of wound, fill the defection of soft tissues, promote the growth and proliferation of the cells around the wound, repair the defection of the soft tissue organs, and promote the wound healing.

The invention provides a neural restoration material, and a preparation method and application thereof. The neural restoration material is characterized in that the neural restoration material contains collagen, polysaccharose substances, active factors and nerve regeneration promoting factors; the neural restoration material is of a three-dimensional reticular porous structure; no immunogenicity exists; the degradation in vivo is realized; the neural restoration material can be in a flaky shape or a hollow tube shape. The neural restoration material is manufactured by small intestine submucosa tissues of pigs; the injured nerve can be effectively protected; the nerve regeneration protection microenvironment is formed; the injured nerve regeneration is promoted; a barrier effect can also be achieved; the injured nerve position is effectively isolated from peripheral tissues; fibroblasts in the peripheral tissues are prevented from invading the injured nerve position.

Described, in certain aspects of the invention, are multilaminate medical graft products, as well as methods for preparing and using the same. An illustrative multilaminate medical graft product of the invention comprises a first layer of remodelable extracellular matrix (ECM) material bonded to a second layer of remodelable ECM material, wherein the first material layer is enriched with a growth factor relative to the second material layer. Such a remodelable ECM material may be comprised of submucosa from a warm-blooded vertebrate, for example, porcine small intestinal submucosa (SIS).

The invention provides a small intestinal submucosa-based vascularization promoting thermosensitive material. The small intestinal submucosa-based vascularization promoting thermosensitive material is prepared by packaging a small intestinal submucosa matrix and a dissolving solution, respectively, wherein the small intestinal submucosa matrix is obtained through the following steps: performing enzymatic hydrolysis, acid dissolution and freeze-drying on the small intestinal submucosa, and then crushing and sterilizing the small intestinal submucosa to obtain a sterile powdery or flocculent small intestinal submucosa matrix material; the dissolving solution consists of phosphate buffer containing NaOH or normal saline. According to the invention, the two matrixes of the small intestinal submucosa-based vascularization promoting thermosensitive material both can be preserved for a long time and are mixed in an appropriate ratio before in use; the thermosensitive material has excellent injectability and is capable of forming a stable gel at 37 DEG C without cross-linking polymerization; the thermosensitive material is proven to have the effect of promoting vascularization by both experiments in vitro and vivo.

The invention provides bionic double-layer dressing, comprising acellular animal intestinal submucosa and a gel layer crosslinked to the same. The acellular animal intestinal submucosa and the gel layer jointly form the bionic double-layer dressing in double-layer structure. Preparation steps include preparing the acellular animal intestinal submucosa, preparing powder of the acellular animal intestinal submucosa, preparing a solution of the acellular animal intestinal submucosa, preparing the bionic double-layer dressing; the solution of the acellular animal intestinal submucosa is mixed witha crosslinking liquid, the mixture is added to the surface of the acellular animal intestinal submucosa, and freezing and crosslinking are performed to obtain the bionic double-layer dressing. The bionic double-layer dressing has excellent tension resistance, has semipermeable barrier, can prevent moisture evaporation and bacterial penetration, is good for healing of a wound, and enables wound healing speed to be evidently increased, with the wound healing speed evidently increased on third to seventh day after surgery.

The invention relates to a uterine cavity implant as well as a preparation method and application thereof. The uterine cavity implant is characterized by having a bagged structure. The uterine cavity implant comprises a substrate material of animal small intestine submucosa subjected to decellularization treatment. An implantable medical device for preventing and treating intrauterine adhesion comprises a biological tissue substrate material. The present decellularization technology for a biological repairing material is improved; compared with the present product, the biological tissue substrate material provided by the invention has the advantages of lower DNA residual quantity, lower immunogenicity, higher anti-infection capacity and higher repairing capacity; and the uterine cavity implant is beneficial to the recovering of tissues, such as, uterine cavity base layer, submucosa and mucous layer. Besides, the invention prepares the biological repairing material into the uterine cavity built-in structure which is used for isolating wound surfaces, repairing uterine cavity tissues and reducing scar forming, so that the intrauterine adhesion problem is solved, and the accordingly generated sterility problem also can be solved.

The invention discloses a method for preparing a biological nanopatch constructed by astragaloside induced BMSC compound polylactic acid-glycolic acid nano-particle modified small intestinal submucosa matrix. The method comprises the following steps: preparing polylactic acid-glycolic acid nano-particles (PLGA) by adopting a multiple emulsion method; preparing a small intestinal submucosa matrix (SIS) having a natural three-dimensional ultra micro-structure by adopting a decellation technology to adsorb the PLGA nano-particles on the surface of SIS and favor adsorption and growth of host cells by means of the SIS after PLGA nano-particle surface modification; inducing BMSC by astragaloside to promote proliferation and differentiation of BMSC and enable the BMSC to compound and grow on the PLGA nano-particle surface modified small intestinal submucosa matrix (PLGA-SIS) of pigs. Therefore, the AS-BMSC-PLGA-SIS biological patch has stable physicochemical property, good biocompatibility and high mechanical strength, and is beneficial to tissue regeneration.

A tissue graft composition is described herein that includes a segment of small intestinal submucosa having at least one nanoparticle incorporated therein such that the permeability of the segment of small intestinal submucosa is altered, thereby providing the segment of small intestinal submucosa with a more substantially uniform structure for cell migration and proliferation. The tissue graft composition may further comprise at least one macromolecule incorporated into the nanoparticle. The tissue graft composition may be utilized in seeded or unseeded methods of tissue repair.

The present invention provides a degradable spherical artificial bladder, which is a medical in situ implantable degradable spherical artificial bladder lined with small intestinal submucosa. The artificial ureteral loop and the artificial urethral loop are reticular pipe structures, and the artificial bladder body is a reticulated spherical structure. The artificial urethral loop, the artificial ureteral loop, and the artificial bladder body are integrally formed, and the small intestinal submucosa is lined inside the three. The invention has a reasonable structural design and fully utilizes the advantages of natural-artificial composite materials. The mesh outer support plays a role of mechanical support in the early stage of implantation, facilitates the suture operation of the surgical port, and can maintain the structure of the urinary bladder for temporary urine storage. functions as well as facilitate fixation to pelvic tissues. The invention is applied in cystectomy operation to replace the resected diseased bladder, and finally form an organ similar to the autologous bladder.

The present invention relates to a method for preparing a biocompatible small intestinal submucosa hydrogel with a controllable in-vivo degradation period, more particularly to a method for preparing a small intestinal submucosa hydrogel which is formed in a solution phase by forming chemical crosslinkages by solubilizing a biocompatible small intestinal submucosa powder and allows the degradation period of a gel formed during in-vivo injection by controlling the degree of crosslinking and a method for preparing a small intestine submucosa drug carrier or a small intestine submucosa support using the hydrogel.

The invention relates to a method for preparing a biomaterial of acellular small intestinal submucosa, which comprises the steps of preposition treatment, acellular treatment, enzyme treatment, preparations of membranous products and particle products. Compared with the prior art, the biomaterial has higher bioactivity and biocompatibility, no obvious immune rejection and no toxic effect on cells; besides, the biomaterial has a certain mechanical strength and toughness, and variable shape, size and thickness, thereby being convenient for clinical suturing and fixing. At the same time, the preparation method has the advantages of unlimited raw material source, cell-free residues, no ethical issues and being capable of effectively inactivating virus. The prepared products are applicable to the biomedical engineering fields, such as repairing defections of body tissue, serving as tissue filling materials, repairing facial depression deformity, serving as tissue reinforcements to replace fascia, repairing membranous defections and malnourished and infected surfaces of wound, serving as materials for biodegradable stents, and serving as injectable filling materials.

The present invention relates to a preparation method for modifying an acellular biological tissue material by using heparin. According to the present invention, the adopted material is mainly a biological tissue material used for cardiovascular repair and other tissue repair, wherein the material comprises vascular walls, pericardium tissues, cerebral dura maters, intestinal submucosa, and the like of various heterogeneous animals; with the preparation method for modifying the heterogeneous acellular biological tissue material for in vivo tissue reconstruction and regeneration by using the heparin, the biological tissue material coated by using the heparin has good performances of good biological compatibility, low immunogenicity, no cell toxicity, thrombus resistance, calcification resistance, anastomotic stenosis resistance, contribution to tissue reconstruction and host endothelial cell regeneration, and the like.

The invention provides a mold for preparing an accellular small intestine submucosa matrix material, which is composed of a base, a pressure frame and a pressure screen, wherein the base is a square plate with bolt holes at four corners; the pressure frame is a hollow square frame with bolt holes at four corners; the pressure screen is a square frame with a square frame structure or reticular structure inside; the bolt holes in the base are corresponding to the bolt holes in the pressure frame one by one; the external diameter of the pressure frame is the same as the dimension of the base; and the inner diameter of the pressure frame is the same as the dimension of the pressure screen. When being used for preparing the accellular porcine small intestine submucosa matrix material, the mold can not compress the space structure of the accellular porcine small intestine submucosa matrix material or destroy the three-dimensional structure of the natural extracellular matrix, and can ensure the porosity of the prepared accellular porcine small intestine submucosa matrix material.

The invention discloses a bionic antimicrobial abdominal wall repair material. The bionic antimicrobial abdominal wall repair material includes a pig small intestine submucosa and a chitosan-elastin fiber mesh layer laminating on one side of the pig small intestine submucosa; and the chitosan-elastin fiber mesh layer is an electrostatic spinning nanoscale chitosan- elastin fiber mesh. According tothe bionic antimicrobial abdominal wall repair material, the laminating combination of the chitosan-elastin fiber mesh layer and the pig small intestine submucosa, so that the antimicrobial ability of the prepared abdominal wall repair material is improved effectively; meanwhile, the mechanical strength and degradation performance of the abdominal wall repair material meet the requirement of abdominal wall repair, angiogenesis can further be facilitated, tissue regeneration is facilitated, the existing technical bottleneck of the abdominal wall repair material is solved conveniently, and thetreatment effect of abdominal wall coloboma repair is effectively improved.

The invention discloses a neiyi enema and use and a preparation method thereof. The neiyi enema comprises the following components: 14 to 41 weight parts of Chinese honeylocust spine, 7 to 22 weight parts of zedoary root, 10 to 49 weight parts of corydalis, 12 to 45 weight parts of sargentodoxa, 3 to 18 weight parts of largetrifoliolious bugbane rhizome, and 12 to 31 weight parts of garden balsamstem. Compared with the prior art, the neiyi enema with the structure has the advantages that: by utilizing the characteristic that intestine submucosa veins are plentiful and have vigorous blood flow, the medicine is directly guided to an illness station and easily absorbed, enhances treatment effect and does not have any side effect; meanwhile, the stimulation of the enema to the intestinal canal can be reduced, and the medicine can be retained in the intestinal canal longer to fully exert the medicinal effect, so that the medicine is easier to absorb and quick in response, and can promote the recovery of the patient, shorten the course of treatment and improve local symptoms; in addition, the enema mainly aims at endometriosis, has antiphlogistic and analgesic effects, also can be usedfor patients suffering from pelvic infection bellyache and salpingemphraxis sterility, and can relieve the inflammation of ovarian ducts and improve pelvic environment to make the patients easily impregnated.

The invention relates to a biological periosteum repair material and a preparation method thereof, and particularly relates to an acellular dermis matrix material and a preparation method and application thereof to periosteum repair. The preparation method comprises: (1) taking a jejunum segment of a pig small intestine, cleaning up, radially cutting open, removing a mucous layer, a myolemma layerand a serosal layer and obtaining a pig small-intestine mucous lower-layer tissue membrane; (2) sequentially carrying out the following processing on the obtained pig small-intestine mucous lower-layer tissue membrane: firstly, placing in peracetic acid solution to soak and cleaning up; then placing in sodium hydroxide solution to soak and cleaning up; finally, placing in acetone solution to soak, cleaning and obtaining a pig small-intestine mucous lower-layer tissue acellular matrix. The biological periosteum repair material is high in biological safety, low in immunogenicity and cytotoxicity, good in biocompatibility, can promote multiplication of cells adhered to the biological periosteum repair material, is applicable to use as a biological repair material and is particularly suitablefor periosteum repair.

The invention provides a small intestinal submucosa-based vascularization promoting thermosensitive material. The small intestinal submucosa-based vascularization promoting thermosensitive material is prepared by packaging a small intestinal submucosa matrix and a dissolving solution, respectively, wherein the small intestinal submucosa matrix is obtained through the following steps: performing enzymatic hydrolysis, acid dissolution and freeze-drying on the small intestinal submucosa, and then crushing and sterilizing the small intestinal submucosa to obtain a sterile powdery or flocculent small intestinal submucosa matrix material; the dissolving solution consists of phosphate buffer containing NaOH or normal saline. According to the invention, the two matrixes of the small intestinal submucosa-based vascularization promoting thermosensitive material both can be preserved for a long time and are mixed in an appropriate ratio before in use; the thermosensitive material has excellent injectability and is capable of forming a stable gel at 37 DEG C without cross-linking polymerization; the thermosensitive material is proven to have the effect of promoting vascularization by both experiments in vitro and vivo.

Described, in certain aspects of the invention, are multilaminate medical graft products, as well as methods for preparing and using the same. An illustrative multilaminate medical graft product of the invention comprises a first layer of remodelable extracellular matrix (ECM) material bonded to a second layer of remodelable ECM material, wherein the first material layer is enriched with a growth factor relative to the second material layer. Such a remodelable ECM material may be comprised of submucosa from a warm-blooded vertebrate, for example, porcine small intestinal submucosa (SIS).

The invention provides a method for preparing copper / zinc mesoporous biological glass-loaded small intestinal submucosa wound surface dressing through a low-temperature three-dimensional printing technique. The method is characterized by comprising the following steps: (1) preparing a small intestinal submucosa (SIS) matrix material; (2) preparing copper and zinc mesoporous biological glass (Cu-MGB / Zn-MGB); (3) compositing SIS with Cu-MGB / Zn-MGB; (4) preparing a wound surface dressing based on the low-temperature three-dimensional printing technique; and (5) carrying out after-treatment process. The SIS wound surface dressing has the advantages that the SIS wound surface dressing has excellent efficacies of resisting bacteria, stopping bleeding, promoting vascularization and accelerating the regeneration rate of the wound surface; the customization design and production can be realized according to different clinic demands; the cost is low, the manufacturing process is stable and controllable, and the large-scale production can be realized; and the wound surface dressing can be stored for a long time and is convenient to use.

The invention discloses a composite guided tissue regeneration membrane and a preparation method thereof, and relates to the technical field of biomedicine. The composite guided tissue regeneration membrane comprises an upper matrix layer, a magnesium alloy membrane and a lower matrix layer which are sequentially arranged from top to bottom, wherein the upper matrix layer and the lower matrix layer are both composed of active ingredients, namely acellular small intestine submucosa matrix materials. The membrane has excellent mechanical properties, can provide enough growth space for bone tissue regeneration, has excellent biological activity, osteoinductivity and degradability, can be maintained in the human body for 8-12 weeks, and provides enough space maintenance time for bone tissue growth, thereby promoting the growth and repair of bone tissue.