379 results about "Composite scaffold" patented technology

The present invention relates to methods of making and using composites and scaffolds as implantable devices useful for tissue repair, guided tissue regeneration, and tissue engineering. In particular, the present invention relates to methods of making and using compression molded resorbable thermoplastic polymer composites which can be subsequently processed with non-organic solvents to create porous, resorbable thermoplastic polymer scaffolds or composite scaffold with interconnected porosity. Furthermore, these composites or scaffolds can be coated with an organic and/or inorganic material.

The invention relates to a kind of laminated gradient composite scaffold material based on the bionic structure and its preparation method. The said material has three or more layers of porous structure, comprising of hyaluronic acid, PLGA, PLA, collagen II and nano-hydroxyapatite (nano-HA) , beta- tricalcium phosphate ( beta-TCP). The upper layer is made by collagen II / hyaluronic acid or PLGA and PLA imitating the cartilage layer. With the counterfeit the calcified cartilage layer in the middle, it is one layer or multi- sublayer, made by nano-HA or beta-TCP with collagen II / hyaluronic acid or PLGA and PLA; the bottom is made of nano-HA or beta-TCP with collagen II or PLGA and PLA. From top to bottom, the content of inorganic material increases in its layers, about 0 to 60 mass percent of layers. The aperture of the scaffold material is 50 to 450 micron, with 70 to 93 percent porosity. The scaffold material made by this invention has an adjustable degradation rate, good mechanical and biocompatible properties, can adapt to culture with cartilage bone cells and compound with growth factor and small molecular or peptides, it can be used to repair cartilage simultaneously.

The invention discloses a multiplex composite engineering scaffold material capable of gradually decomposing bone tissue and a preparation method thereof. The composite scaffold material consists of calcium phosphate bone cement, biological compatible degradable synthetic high polymer and biological compatible degradable natural high polymer, has better mechanical property and gradient degradation characteristic, and can achieve the aim of regenerating and repairing bone tissue defect by implanting a bone growth factor to induce in-vivo stem cells to be differentiated into bone cells, thereby obviously improving initial strength and toughness of the scaffold material, and ensuring enough strength and toughness of the scaffold material during operating and implanting. After compounded with the high polymer material, the scaffold has excellent flexibility, so that the scaffold can be subjected to certain machining, such as cutting and the like.

A fiber-microsphere composite scaffold including a first layer of material selected from one of a layer of bioresorbable microspheres and a layer of bioresorbable fibers; and a second layer of material selected from the other of the layer of bioresorbable microspheres and the layer of bioresorbable fibers. A fiber-microsphere composite scaffold reduced pressure tissue treatment apparatus is also included as well as methods for making fiber-microsphere composite scaffolds.

The invention relates to a method used for preparing a microcarrier/polymer composite scaffold by electro-deposition. The method comprises following steps: the surface of a microcarrier is modified, so that the surface of the microcarrier is positively charged; the microcarrier is loaded with a bioactive component so as to obtain the functional microcarrier; the functional microcarrier is delivered into an organic solvent, and the mixture is treated by ultrasonic and is stirred so as to obtain an electro-deposition solution with a concentration of 0.1 to 1.0mg/ml; a polymer scaffold is prepared, an electrode provided with the polymer scaffold is taken as a cathode, and a blank electrode is taken as an anode; the cathode and the anode are delivered into the electro-deposition solution, and the electro-deposition solution is stirred for electro-deposition so as to obtain the composite scaffold; and the composite scaffold is washed and dried in the air so as to obtain the microcarrier/polymer composite scaffold. Preparation time is short; reaction conditions are mild; and it is impossible to cause early release or inactivation of the bioactive component loaded on the microcarrier. The microcarrier/polymer composite scaffold is capable of simulating the multiple interaction of cells in the body, ECM and growth factors, and providing an ideal environment for tissue therapy and tissue repair.

The present invention provides a method for achieving assembly of a macroscopic size nanometer material and obtaining a series of functional nanometer composite scaffolds by adopting a chitosan scaffold as a matrix, wherein the series of the functional nanometer composite scaffolds are obtained by assembling various functional nanometer materials in a chitosan scaffold. The method specifically comprises that: unidirectional freezing is performed to obtain a chitosan porous scaffold, the chitosan porous scaffold is immersed into a nanometer material aqueous solution, and the nanoparticles are adsorbed onto the surfaces of pore channels inside the chitosan porous scaffold so as to obtain the nanometer composite scaffold; or a nanometer material is directly mixed in a chitosan solution before a chitosan porous scaffold is obtained, and then unidirectional freezing is performed to obtain the nanometer composite chitosan porous scaffold. The method of the present invention has characteristics of simple operation and wide applicability, and can be applicable for mass preparation of required products. According to the nanometer composite scaffold of the present invention, the reversible shape resilience performance of the original chitosan porous scaffold is provided, and the functionality of the nanometer material is provided for the final macroscopic scaffold, such that important guiding effects are provided for applications of various functional nanometer materials in optics, electricity, magnetism, thermotics, biomedicine, and other fields.

The present invention provides biocompatible composite materials that can be fabricated into a scaffold having properties suitable for bone repair and regeneration. These scaffolds have sufficient mechanical strength to be useful for the repair and regeneration of cortical bone.

The present invention discloses a preparing method of the calcium phosphate cement/chitosan-gelatin composite scaffold, the process is as follows: mixing the alpha- tricalcium phosphate, monohydrate calcium biphosphate, hydroxyapatite and calcium carbonate according to the mass ration and rubbing, then obtaining the calcium carbonate cement solid powder; mixing the disodium hydrogen phosphate solution and the cement solid powder in the mass ratio and stirring to a mash, then filling into a mould, vacuum solidifying and mold releasing, after that, solidifying again and obtaining the calcium phosphate cement scaffold; subsequently, dissolving the chitosan and the gelatin into the acetic acid water solution, configuring the chitosan-gelatin solution and adding the glutaraldehyde water solution; putting the calcium phosphate cement scaffold into the chitosan-gelatin solution, in vacuum condition, filling chitosan-gelatin solution into the calcium phosphate cement scaffold, after that cooling and drying, obtaining the calcium phosphate cement/chitosan-gelatin composite scaffold. The prepared calcium phosphate cement/chitosan-gelatin composite scaffold has a perfect mechanical property and biological property.

The invention discloses scaffolds of umbilical cord decellularized Wharton jelly for tissue engineering and a preparation method thereof. Umbilical cords are employed as the raw material and their outer membranes and vascular tissues are peeled off. And the rest part of the umbilical cords is subjected to hypotonic freeze-thaw, mechanical pulverization, differential centrifugation, enzymatic digestion for decellularization. Then the umbilical cord Wharton jelly is collected and injected into a mold. After freeze drying and crosslinking, multiple three dimensional porous sponge scaffolds and composite scaffolds can be obtained. The method of the invention has the advantages of wide material source, low cost, simple technology. And the prepared scaffolds are characterized by controllable fine structure, appropriate degradation rate, good biocompatibility, and biomechanical strength, which are in favor of cell adhesion and the uniform distribution of seed cells within the scaffolds, as well as seed cell multiplication, migration and growth. Thus, the scaffolds of umbilical cord decellularized Wharton jelly in the invention can be widely applied in the tissue engineering field such ascartilage, bone, skin and nerve, with a favorable clinical application prospect.

The invention relates to a preparation method and application of a silicon rubber/collagen-based porous skin scaffold material. The preparation method comprises the following steps of: (1) putting an organic silicon film into a plasma treatment instrument, and introducing a polar group into the surface of silicon rubber; (2) preparing collagen solution or mixed solution of collagen and other biomacromolecules at certain concentration, coating the solution on the surface of a silicon rubber film obtained in the step (1), and extracting a solvent by using a freeze dryer; (3) soaking a silicon rubber/collagen-based composite scaffold obtained in the step (2) in a dehydrating agent-containing mixed solvent of ethanol and water, reacting for 6 to 12 hours at room temperature, and soaking and washing by using a large amount of deionized water; and (4) putting a scaffold obtained in the step (3) into the freeze dryer again to extract absorbed water. The silicon rubber/collagen-based composite porous material prepared by the method has high biocompatibility and appropriate pore diameter, is stable, and can be used for a skin scaffold, or a temporary dressing favorable for repairing wound parts.

The present invention discloses an injectable-porous-drug loaded polymethyl methacrylate-based composite scaffold bone transplant material and a preparation method thereof, and belongs to the field of organic functional materials preparation. The polymethyl methacrylate-based composite scaffold bone transplant material uses polymethyl methacrylate (PMMA) as a scaffold for providing mechanical supporting, and a chitosan-based thermosensitive hydrogel as a pore forming agent and an osteoconductive material and a drug carrier, and the polymethyl methacrylate (PMMA) and the chitosan-based thermosensitive hydrogel are mixed with each other to form an injectable-porous three-dimensional structural bone cement composite. The scaffold bone transplant material is simple in preparation method, suitable in reaction temperature, and good in biocompatibility, has matched mechanical properties, good biological mineralization and corresponding anti-bacterial, anti-inflammatory or anti-tumor capabilities, and has broad prospects in clinical application of reconstruction of bone tissues in future.

A composite chitosan/nano-hydroxyapatite microsphere-based material used for bone-grafting and delivery of therapeutic agents. The composite material may be produced using co-precipitation methods. The composite material may be used to form scaffolds with significantly greater surface area and surface roughness than scaffolds composed of only chitosan. Composite scaffolds exhibit less swelling and greater toughness and flexibility than scaffolds fabricated by other techniques. Composite scaffolds also exhibit greater osteoblast proliferation. Composite scaffolds also may contain therapeutic agents or medicaments, and may be lyophilized.

The invention discloses a porous calcium phosphate/natural polymer composite scaffold, a preparation method and application thereof. The method comprises the following steps: firstly utilizing 3D printing to manufacture a polymer support in which holes are completely connected as a sacrifice model, putting the sacrifice model into a mold, then uniformly mixing natural polymer microspheres with calcium phosphate bone cement to prepare a slurry, pouring the slurry into the model so that the holes of the model are filled with the slurry, then using a solvent to remove the polymer model so as to obtain the connected porous calcium phosphate/natural polymer composite scaffold. Combined with the characteristics of bone cement such as low-temperature self-setting and good mechanical property andthe characteristics of natural polymer such as high biological activity and easy degradation, the three-dimensional connected macroporous structure composite scaffold is prepared. After the scaffold is planted into the body, the natural polymer microspheres are rapidly degraded so as to form a hierarchical pore structure in situ. High-temperature processing is not needed in the preparation process, the biological activity and mechanical property are good, the structure and degradation rate are controllable, the ingrowth of bone tissues and blood vessels is facilitated, and the bone repair effect is improved.

The invention relates to a 3D-printed multi-structure bone composite scaffold. The 3D-printed multi-structure bone composite scaffold comprises a multi-layer structure, wherein different layers are made of composite materials in different proportions through 3D printing, and the 3D-printed multi-structure bone composite scaffold has different 3D printing fiber spacings and porosity factors. The specific structure comprises a bionic bone structure, the outer layer is low in porosity and small in aperture to simulate a compact bone structure, the inner layer is high in porosity and large in aperture to simulate a cancellous bone structure, and the scaffold similar to a real bone structure is integrally formed; in an osseointegration structure, the outer layer is high in porosity and large inaperture so as to promote integration with surrounding bones, the inner layer is low in porosity and small in aperture so as to support the overall structure while promoting osseointegration, and thewhole structure is suitable for repairing bone defects. The material of the scaffold is preferably a composite material of tricalcium phosphate (TCP) and polycaprolactone (PCL), and the scaffold hasgood biocompatibility and printability. The bone repair effect is promoted by adding metal ions and performing surface modification treatment.

Implantable, biocompatible scaffolds containing a biocompatible, porous, polymeric matrix, a biocompatible, porous, fibrous mat encapsulated by and disposed within said polymeric matrix, and a plurality of mammalian cells seeded within said tissue scaffold. The invention also is directed to methods of treating disease or structural defects in a mammal utilizing the scaffolds of the invention.

The embodiments herein disclose a method of fabricating composite scaffolds for skin tissue regeneration. The methacrylated hyaluronic acid (HAMA) and methacrylated gelatin (GelMA) are synthesized. The poly (glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) microfibrous scaffolds are synthesized. The hydrogel is synthesized. The composite scaffold comprising hydrogel and poly (glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) microfibrous scaffolds is fabricated. A plurality of physico-chemical characteristics of the composite scaffold comprising hydrogel and poly (glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) microfibrous scaffolds are analysed. The physico-chemical characteristics comprises mechanical properties, swelling ratio and enzymatic degradation and scanning electron microscope imaging. The fibroblast cells are encapsulated within the composite scaffold comprising hydrogel and poly (glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) microfibrous scaffolds and hydrogels. The fibroblast cells are seeded on composite scaffold and PGS-PCL scaffold. The fibroblast cell viability, fibroblast cell attachment, fibroblast cell spreading, fibroblast cell proliferation and fibroblast cell metabolism are analysed in composite scaffolds, PGS-PCL scaffolds and hydrogels.

The invention discloses a biological composite scaffold for repairing bone defect. The composite scaffold is prepared from nano hydroxyapatite and silk fibroin, wherein the mass ratio of the nano hydroxyapatite to the silk fibroin is 90:10-60:40. The composite scaffold is porous, the porosity is 70 to 95 percent, the aperture size is 100 to 600 microns, pores are mainly circular, and the pores are mutually communicated. Aqueous solution of the de-gummed silk fibroin is used as a carrier, silk fibroin/nano hydroxyapatite composite sol is prepared by a co-precipitation method, the silk fibroin/nano hydroxyapatite composite scaffold is prepared by combining ion percolation and freeze drying process, and the distribution of aperture in the composite scaffold is controlled by adjusting the radius of NaCl particles and freezing parameters. Compared with the prior art, the scaffold has the advantages of easily-controlled porosity and the aperture distribution, and good mechanical property.

The invention discloses a hydroxyapatite-based biological composite scaffold composed of hydroxyapatite, silk fibroin and chitosan, wherein a mass ratio of the components is represented by that: HA:SF:CS=(60-80):(10-20):(10-20). The composite scaffold is in a porous shape, wherein the porosity is 70 to 90%, and the pore size is 150 to 200mun. The pores are basically round, and are communicated with each other. The invention also discloses a tissue engineered bone constructed by using the hydroxyapatite-based biological composite scaffold. According to the invention, BMSCs transfected with VEGF gene is implanted into the hydroxyapatite-based biological composite scaffold, such that the tissue engineered bone is formed. According to the invention, hydroxyapatite, silk fibroin and chitosanare are adopted as raw materials, and a porogen-leaching technology is combined with a vacuum drying technology, such that the hydroxyapatite-based biological composite scaffold material is prepared. A degradation rate of the scaffold is regulated through the regulation of the proportions of the components. The pore size distribution of the composite material is controlled through the regulations of the radiuses and the addition amount of sodium chloride particles.

The invention relates to a method for producing a multi-layer collagen scaffold. The method generally comprises the steps of: preparing a first suspension of collagen and freezing or lyophilising the suspension to provide a first layer; optionally preparing a further suspension of collagen and adding the further suspension onto the layer formed in the previous step to form a further layer, and freezing or lyophilising the layers, wherein when the layer formed in the previous step is formed by lyophilisation the lyophilised layer is re-hydrated prior to addition of the next layer; optionally, repeating the aforementioned step to form one or more further layers; and preparing a final suspension of collagen and pouring the final suspension onto the uppermost layer to form a final layer, and freeze-drying the layers to form the multilayer collagen composite scaffold.

The invention discloses a high-strength continuous gradient composite scaffold, and relates to the field of biomedical materials. The composite scaffold consists of hydrogel and magnetic composite nano particles; the weight percentages of the magnetic composite nano particles at the top and the bottom of the high-strength continuous gradient composite scaffold are respectively 0 percent and 8 to 70 percent; the weight percentage of the magnetic composite nano particles is gradually increased from the top of the high-strength continuous gradient composite scaffold to the bottom to form continuous gradient distribution; and the hydrogel is hydrogel of polyvinyl alcohol or hydrogel of polyvinyl alcohol and a natural material. The high-strength continuous gradient composite scaffold solves the problem of interface defect of the conventional multilayer scaffold, and can keep mechanical property and biological function and well meet the multilevel requirement of an osteochondral natural structure. The invention also discloses a preparation method for the high-strength continuous gradient composite scaffold. The preparation method is easy to implement and control, and has a broad application prospect.

The invention provides a calcium phosphate/collagen/bone-like apatite three-level bionic bone tissue engineering scaffold and a preparation method thereof. The material of the scaffold possesses components similar to natural bone tissues, and an inorganic/organic/inorganic three-level bionic bone-like netted three-dimensional structure. The preparation method comprises: customizing a calcium phosphate ceramic which possesses a bionic porous structure similar to the natural bone micropore structure, filling collagen prepared by simulated body fluid (SBF) into the porous calcium phosphate ceramic base, low-temperature aging to simulate a biomineralization process, in-situ nucleating on the collagen organic macro-molecule matrix, and self-assembly crystallizing to form a third level bone-like apatite layer structure. The composite scaffold material has bionic material components and bionic microstructure both similar to the natural bone tissues, the mechanical properties and the biological activity of the material are substantially improved, and the composite scaffold material has a wide application prospect.

The invention provides an integrated three-layer composite scaffold for repairing joint cartilages, and a production method thereof. The composite scaffold comprises a cartilage layer, a calcified subchondral bone plate layer and a spongy bone layer which are sequentially superposed from bottom to top, the composite scaffold is formed in a one-shot manner through three-dimensional printing, the cartilage layer is a sodium alginate hydrogel porous layer, the calcified subchondral bone plate layer is a biological ceramic particle and sodium alginate composite hydrogel compact layer, the spongy bone layer is a biological ceramic particle and sodium alginate composite hydrogel porous layer, and the sodium alginate components in all above layers undergo ionic cross-linking to form an integrated structure, so every layer of the composite scaffold has a specific composition and a specific structure, the composite scaffold has good interlayer binding force, and the integral structure stability of the composite scaffold is improved. The invention also provides the production method of the integrated three-layer composite scaffold for repairing joint cartilages. The method has the advantages of simplicity, easiness in enforcement, easiness in control of technologic parameters, acceleration of the promotion and application of a three-dimensional printing technology in the biologic field, and wide market prospect.

The invention relates to a ligament-bone composite scaffold with a biomimetic connecting interface and a forming method thereof. The forming method comprises the following steps: firstly simulating a natural ligament-bone interface structure and utilizing a rapid forming technology to manufacture a resin negative type of a bone scaffold model with a fiber connecting structure; pouring a bone scaffold material solution into the resin negative type, and performing freeze-drying and high-temperature sintering to manufacture a bone scaffold with an internal communication pipeline and the fiber connecting structure; then primarily connecting ligament fiber with the fiber connecting structure of the bone scaffold, and fixing a die used for manufacturing of a biomimetic interface with a ligament-bone scaffold formed by primary connection; pouring the ligament material composite solution with the bone scaffold material in various changes of concentration into the interface of the ligament and the bone scaffold as secondary connection; and performing freeze-drying and removing the die, so as to obtain the ligament-bone composite scaffold with the biomimetic interface. According to the invention, the transmission of nutrients and metabolites is facilitated, the connecting strength of the ligament-bone composite scaffold is improved, and the ingrowth of cells after implantation is facilitated.