96 results about "Composite scaffold" patented technology

The invention provides a pourable tumor organoid vascularization model and a construction method thereof, and relates to the technical field of organoid culture. A temperature-sensitive degradable material and a photo-crosslinking modified temperature-sensitive material are used, the composite stent is printed through two channels, a multi-component gel mask partition curing process is combined, the stability of a perfusion pipeline is improved, and a partitioned highly-bionic complex microenvironment is accurately constructed in a macroscopic three-dimensional space; and constructing to obtain the perfusion tumor organ vascularization model. The model is high in bionic degree and stable in structure, can be applied to deepening cell and microenvironment interaction research and tumor-related drug screening, and has a good application prospect.

The invention provides a developmental growth plate layered stent as well as a preparation method and application thereof, and belongs to the technical field of bone tissue engineering materials. The developmental growth plate layered stent provided by the invention is divided into three layers, the upper layer is a cartilage cell layer, the middle layer is a blood vessel barrier layer, and the lower layer is an ossification layer. The blood vessel barrier layer can be used as biological glue to bond the upper layer and the lower layer, so that the composite stent is stable in structure and not easy to break; the upper layer contains bone marrow mesenchymal stem cells (BMSCs), and the middle layer and the lower layer are free of cells. Under the condition of in-vitro chondrogenesis induction culture, the BMSCs on the upper layer can penetrate through the middle layer and be colonized on the lower layer, and in the process, part of the BMSCs can be differentiated into cartilage cells. The middle layer containing the platelet reaction protein 1 (TSP1) has a specific intervention effect on cell migration, which is expressed by inhibiting the migration of vascular endothelial cells, but has no influence on the migration of the BMSCs.

The invention provides a multi-nozzle 3D printing composite scaffold capable of promoting bone regeneration as well as a preparation method and application thereof, the multi-nozzle 3D printing composite scaffold is prepared by adopting multi-nozzle collaborative printing of a 3D printing system, the multi-nozzle 3D printing composite scaffold comprises a synthetic polymer material printed by a high-temperature nozzle and a hydrogel material printed by a low-temperature nozzle, and microfilament patterns formed by the two materials are alternately arranged. Wherein the synthetic polymer material is prepared from polycaprolactone and magnesium oxide nano particles; the hydrogel material is prepared from methacrylated gelatin, methacrylated gilan gum and double drug loading nano particles. The 3D printing composite scaffold provided by the invention has excellent mechanical properties, the double-drug-loaded nanoparticle sequence released osteogenesis promoting and vascular promoting drug coupling osteogenesis-vascularization promoting effects, released magnesium ions synergistically enhance osteogenesis and vascularization performance, and the 3D printing composite scaffold has a good bone defect repair effect and has application potential in the field of bone tissue repair.

The present invention discloses an integrated bone and cartilage scaffold with a directional pore structure and a preparation method thereof, which belongs to the fields of tissue engineering, biomedicine and biomanufacturing technology. The method simulates the natural material and structure of bone cartilage, uses the principle of directional crystallization to prepare a bone and cartilage scaffold with a directional structure, and uses a low-temperature deposition manufacturing process based on rapid prototyping technology to prepare a horizontally oriented cartilage layer scaffold and a vertically oriented subchondral bone layer scaffold. Between the cartilage layer and the subchondral bone layer, methacrylated gelatin capable of UV cross-linking is combined with other materials with chemical grafting and bonding effects as a transition layer, thereby preparing a tissue engineering bone and cartilage integrated composite scaffold. The present invention is aimed at the treatment of articular bone and cartilage defects, and uses a bioactive material to manufacture a bone and cartilage composite scaffold with a directional pore structure. It can promote the coordinated repair of bone and cartilage tissue according to the characteristics of different functional areas of bone and cartilage, thereby improving the repair effect of bone and cartilage defects.

Artificial meniscal scaffolds characterized by a composite of circumferential polymer fiber network and orthogonal polymer fiber network embedded in an arcuate bioresorbable matrix comprised of collagen and hyaluronic acid. The orthogonal polymer fiber network prevents separation of the circumferential polymer fiber networks. The polymer fiber networks convert axial compressive forces on the scaffolds to tensile loads on the circumferential polymer fibers. The composite scaffold can be anchored to bone by novel anchoring components that protect the polymer fibers and ensure immediate securement of the artificial meniscal scaffold to bone.

The application discloses a kind of hydrogel filled polycaprolactone composite material scaffolds and its preparation method and application, the polycaprolactone composite material scaffold has porous structure, wherein the surface and internal pore are interconnected, and the aperture from surface to inside is gradiently larger;The pore of the polycaprolactone composite material scaffold is filled with drug-loaded gel composite material.The hydrogel filled polycaprolactone composite material scaffold of the application prints scaffold and has macroscopic macropore and microscopic small hole simultaneously, not only can improve the surface roughness of scaffold, reach the purpose of significantly promoting scaffold cell adhesion, also can significantly improve the specific surface area of scaffold, make polycaprolactone accelerate degradation and release more acidic degradation product, solve the problem that polydopamine is slow in degradation in vitro and in vivo.The application fills good hydrogel in the pore of printing scaffold, can further regulate the hydrophilicity and hydrophobicity of material while maintaining the mechanical strength of material, so that it is more suitable for the regeneration and repair of bone defect.

The disclosed composite scaffold provides a highly porous and flexible structure that substantially maintains its three-dimensional shape under tension and provides mechanical reinforcement of the repair or reconstruction-first via scaffold mechanical properties, and subsequently, through newly regenerated functional tissue as the scaffold is resorbed.

The invention belongs to the technical field of biomedical materials, and discloses an anisotropic nanofiber yarn composite scaffold as well as a preparation method and application thereof. The anisotropic nanofiber yarn composite scaffold is formed by compounding methylacryloyl modified gelatin and a nanofiber yarn scaffold prepared through electrostatic spinning. The anisotropic nanofiber yarn composite scaffold not only has more excellent mechanical properties, but also can provide a more suitable microenvironment for cell adhesion and growth, can significantly improve the remodeling efficiency of an extracellular matrix, and has a wide application prospect in the field of heart valve repair.

The invention discloses a preparation method and application of a functional degradable soybean oil-based bionic porous composite bone scaffold. The method comprises the following steps: taking epoxidized soybean oil acrylate as a bio-based base material, compounding isobornyl methacrylate to improve the defects of high viscosity and low photosensitivity of the bio-based base material, adding a photoinitiator containing ethyl 2, 4, 6-trimethylbenzoyl phenyl phosphonate and phenyl bis (2, 4, 6-trimethylbenzoyl) phosphine oxide, compounding, and carrying out aging treatment to obtain base resin; mixing calcium lignosulphonate into the base resin to prepare composite photosensitive resin, and printing a triple-period extremely-small curved surface structure through a digital light processing technology and a 3D printing technology to finally obtain the functional degradable soybean oil-based bionic porous scaffold. The scaffold has calcium lignosulphonate-mediated controllable photo-thermal response behaviors of near-infrared light illumination regulation and bone scaffold component dependence under the condition of simulating the in-vivo environment of a human body, so that a controllable photo-thermal response-mediated remote shape memory function is realized, and meanwhile, a mild, safe and controllable photo-thermal curative effect can also be realized. Besides, calcium lignosulphonate promotes degradation of the composite scaffold by preferentially dissolving and generating a microporous structure on the surface of a matrix while improving the hydrophilic performance of the scaffold, and continuously releases calcium ions, so that the scaffold shows enhanced biomineralization ability and promotes cell proliferation and osteogenic differentiation. The research provides a solution with potential for the soybean oil-based biological photosensitive material in the digital light processing preparation technology and the multi-functional application of the bionic porous bone scaffold so as to deal with complex and irregular bone defects.

The invention relates to a living plant cell-animal cell composite scaffold as well as a preparation method and application thereof. The living plant cell-animal cell composite scaffold comprises a 3D printing hydrogel scaffold used as a muscle repair carrier and a 3D printing biological ceramic scaffold used as a bone repair carrier, wherein the 3D printing hydrogel scaffold and the 3D printing biological ceramic scaffold are assembled by simulating a muscle-skeleton structure; the hydrogel scaffold comprises a polymer matrix component, and functional plant cell components, namely diatom cells and mammalian cell components, namely muscle stem cells, which are distributed in the polymer matrix component, the biological ceramic scaffold comprises a biological ceramic scaffold matrix component and mammalian cell component bone marrow mesenchymal stem cells inoculated and loaded on the biological ceramic scaffold matrix component.

The invention discloses an antibacterial hydroxyapatite fiber-gelatin composite scaffold as well as a preparation method and application thereof, the composite scaffold has the structural characteristics of regular spherical macropores and communicating pores, and the interior of the composite scaffold is formed by mutually interwoven antibacterial hydroxyapatite fiber-gelatin composite fibers; the preparation method comprises the following steps: injecting antibacterial hydroxyapatite fiber / gelatin turbid liquid into a paraffin ball template under negative pressure, and carrying out thermal cooling phase separation and a paraffin ball template leaching technology. The composite scaffold integrates multiple functions of active antibiosis, osteogenic differentiation promotion, neovascularization and bone tissue ingrowth and the like, and has huge application potential in the field of promotion of rapid repair and regeneration of infectious bone defects.

The application discloses a kind of bionic bone / cartilage double-layer composite scaffold material and its preparation method and application.The bionic bone / cartilage double-layer composite scaffold material of the application is composed of upper cartilage repair hydrogel layer and lower bone repair scaffold layer.The main feature of the application is that by radial freezing, the chitosan-chitin whisker liquid crystal composite hydrogel micron radial channel with diameter gradient change is constructed in the lower scaffold, which can promote the transport of nutrients, cell recruitment, migration and adhesion;At the same time, the drug-loaded liquid crystal hydrogel constructed in the longitudinal large channel of the scaffold and the upper cartilage repair layer can realize the sustained release of active drugs, so as to further regulate stem cell osteogenesis / cartilage differentiation and promote the generation of blood vessels, and is expected to have good application prospect as biomedical materials such as bone / cartilage tissue repair materials.

The invention discloses a modified silk fibroin artificial blood vessel and a preparation method and application thereof.The modified silk fibroin artificial blood vessel comprises a modified silk fibroin composite scaffold and a polyurethane layer, the polyurethane layer is arranged on the modified silk fibroin composite scaffold through electrostatic spinning, and the polyurethane layer is prepared from polyurethane, the modified silk fibroin composite scaffold is prepared from the following raw materials: modified silk fibroin, arginine-glycine-aspartic acid polypeptide modified polycaprolactone, bacterial cellulose, a growth factor and glutaraldehyde. The modified silk fibroin is in bridge connection with the arginine-glycine-aspartic acid polypeptide modified polycaprolactone and is loaded with the growth factor. The comprehensive biocompatibility of the artificial blood vessel can be improved, thrombosis and intimal hyperplasia are effectively inhibited, and therefore the long-term patency rate of the small-caliber artificial blood vessel is greatly increased.

The application discloses a modified polycaprolactone composite scaffold and a preparation method and application thereof, and the polycaprolactone composite scaffold has a porous structure, wherein the pores on the surface and inside are interconnected, and the pore diameter is gradually increased from the surface to the inside; the surface of the polycaprolactone composite scaffold is loaded with metal organic framework materials loaded with antibacterial drugs inside. The modified polycaprolactone composite scaffold of the application has macro-pores (fiber gaps) and micro-pores (fiber surface holes) at the same time, can not only improve the surface roughness of the scaffold, achieve the purpose of significantly promoting cell adhesion of the scaffold, but also can significantly improve the specific surface area of the scaffold, accelerate the degradation of polycaprolactone and release more acidic degradation products, thereby accelerating the degradation of polydopamine and accelerating the release of the drugs embedded inside, and the problem that the polydopamine is slow in degradation in vivo and in vitro is solved.

The disclosed composite scaffolds provide a highly porous and flexible structure that substantially maintains its three-dimensional shape under tension and provides mechanical reinforcement to the repair or reconstruction, first through the mechanical properties of the scaffold and then through the newly regenerated functional tissue as the scaffold is resorbed.

The present application relates to the technical field of biological medicine, in particular to a kind of polydopamine and collagen composite scaffold and its preparation method and application, preparation method includes the following steps: (1) dopamine hydrochloride is added to the deionized water containing appropriate amount of ammonia and anhydrous ethanol, stirring at room temperature, to obtain crude polydopamine. (2) the crude polydopamine is centrifuged at low speed, and then high-speed centrifugation is carried out, and the precipitate is collected;After cleaning, freeze-drying is carried out, to obtain purified polydopamine. (3) then weigh appropriate amount of polydopamine and add to collagen solution, adjust pH, stirring at room temperature, freeze-drying, to obtain polydopamine and collagen composite scaffold. The polydopamine and collagen composite scaffold of the present application are prepared by physical blending rather than chemical modification strategy, which not only maintains the biocompatibility of the composite scaffold but also greatly reduces the production cost, and the simple preparation process provides feasibility guarantee for clinical conversion, and the composite scaffold can be used for the treatment of diabetic wounds.

This invention discloses a magnesium alloy composite scaffold, its preparation method, and its application. The composite scaffold comprises a JDBM magnesium alloy scaffold with a highly interconnected porous structure and a composite hydrogel layer filling and encapsulating it. The hydrogel layer is formed by photocrosslinking and curing of methacrylamide-loaded glucose oxidase. This composite scaffold is a bio-battery-like therapeutic system. GOx catalyzes the biocatalytic oxidation of glucose to hydrogen peroxide, while magnesium alloy acts as an electroactive anode, together forming an adaptive electrochemical cascade reaction system. Through spontaneous oxidation dissolution and short-circuit electrochemical reactions, it rapidly removes hydrogen peroxide into water and oxygen while simultaneously promoting the oxidation of magnesium. 2+ Release. Therefore, this system not only transforms the harmful high-glucose microenvironment of diabetes into a microenvironment conducive to bone regeneration, but also achieves multiple synergistic effects of anti-inflammation, anti-oxidation, and bone promotion through the continuous supply of magnesium ions and the timely removal of H2O2, significantly promoting the repair and regeneration of bone defects under diabetic conditions.

This invention provides a calcium phosphate-polycaprolactone composite scaffold, its preparation method, and its applications. The raw materials for preparing the calcium phosphate-polycaprolactone composite include polycaprolactone, β-tricalcium phosphate, and hydroxyapatite. The resulting composite scaffold possesses functions of promoting immunity and regulating bone regeneration. The preparation method of this invention involves first forming a hydroxyapatite / polycaprolactone composite film, which is then broken down and recombined with β-tricalcium phosphate to form a composite solution. The resulting 3D-printed composite scaffold not only possesses good mechanical strength and biocompatibility but also induces stem cell proliferation and osteogenic differentiation, and induces macrophage regeneration and polarization. It can be used to regulate immune cell responses, induce a bone immune microenvironment conducive to bone formation, thereby enhancing bone regeneration, and can be used for the repair and regeneration of tissue defects.

The invention is suitable for the technical field of biomedical materials, and provides a luteolin chemical grafting-based composite scaffold as well as a preparation method and application thereof. Polycaprolactone glycol, polyethylene glycol and luteolin form a high-molecular polymer through a chemical grafting method, and the high-molecular polymer is prepared into a stent form through 3D printing. The preparation method is simple in synthesis process and low in cost, and the luteolin chemically-grafted polycaprolactone glycol / polyethylene glycol high-molecular polymer composite scaffold is constructed through carbamate bonds, so that uniform dispersion and slow release of luteolin in the scaffold are realized; the problems that luteolin is poor in water solubility, high in metabolism speed and prone to agglomeration in physical blending are solved, immunoregulatory bone regeneration is achieved by means of the function that luteolin regulates and controls M2 type polarization of macrophages, and meanwhile the surface hydrophilicity of the polymer composite scaffold is improved. The composite scaffold can be applied to the biomedical field of bone defects in an immune microenvironment abnormal state and the like, and repair of a bone defect area is promoted through the effects.

This invention discloses a composite scaffold for promoting bone regeneration in diabetic patients, its preparation method, and its applications, relating to the field of medical biomaterials technology. The composite scaffold for promoting bone regeneration in diabetic patients comprises: a three-dimensional porous framework; and a bioactive hydrogel layer loaded with naringin. The bioactive hydrogel layer loaded with naringin is coated on the surface and pore walls of the three-dimensional porous framework. The technical solution provided by this invention uses a 3D-printed PLGA / HA porous framework to provide mechanical support and osteoconductivity, and a supramolecular hydrogel coating loaded with naringin to endow it with immunomodulatory and drug sustained-release functions. This scaffold has good hydrophilicity and biocompatibility, and can effectively induce macrophage polarization towards the M2 anti-inflammatory phenotype by activating the JAK-STAT signaling pathway, improving the local inflammatory microenvironment of diabetic bone defects. Simultaneously, it indirectly promotes vascular endothelial cell migration, lumen formation, and osteogenic differentiation of bone marrow mesenchymal stem cells by regulating macrophages.

The application discloses a kind of biomimetic composite scaffold with periosteum-cortical bone-cancellous bone structure and its preparation method and application.The scaffold is assembled by independent periosteum biomimetic scaffold module and independent bone trunk biomimetic module.The periosteum biomimetic scaffold module is cylindrical tube structure hydrogel, loaded with zinc silicate particles and periosteum related cells, with the biological functions of promoting cartilage differentiation, secreting nerve and vascular factors and synthesizing periosteum matrix proteins.The bone trunk biomimetic scaffold module is cylindrical bioceramic, with multiple vertical pipes embedded in the dense layer of outer wall to simulate cortical bone, and with staggered structure inside to simulate cancellous bone.Animal experiments show that the biomimetic composite scaffold can effectively promote the integrated regeneration of bone trunk and periosteum in rabbit radial critical size defect model.Therefore, the application provides a new solution for the biomimetic design and regeneration repair of bone tissue engineering.

The invention relates to a chiral hydroxyapatite composite biological material with a multilayer structure as well as preparation and application of the chiral hydroxyapatite composite biological material. The biological material is prepared from a CS / CA-CMHAP composite scaffold and silver ions (Ag < + >) loaded on the CS / CA-CMHAP composite scaffold, wherein the CS / CA-CMHAP composite scaffold is obtained by compounding CS, CA and a hydroxyapatite nanosheet CMHAP. Compared with the prior art, the biological material provided by the invention has a remarkable healing promoting effect on old-age infected skin wounds in an in-vivo experiment, and can effectively reduce inflammatory response, promote tissue regeneration and the like by regulating and controlling an immune microenvironment.

The invention discloses a functional decellularized porcine cornea, a decellularized porcine cornea composite scaffold containing layered slow-release bioactive molecules and a preparation method of the functional decellularized porcine cornea composite scaffold. The preparation method of the functionalized decellularized pig cornea comprises the following steps: (1) pretreating raw materials; (2) carrying out decellularization treatment; (3) carrying out functionalization treatment on the decellularized porcine cornea and the like. Researches show that the functionalized decellularized porcine cornea prepared by the method disclosed by the invention can well load bioactive molecules. In addition, the functionalized decellularized porcine cornea prepared by the method disclosed by the invention also has better mechanical properties. Furthermore, based on the functionalized decellularized porcine cornea, a layered sustained-release design and a 3D printing customization technology are adopted, the cornea scaffold with anti-inflammatory, regeneration promotion and neural restoration functions is constructed, breakthrough is achieved in the aspects of immune compatibility, mechanical properties and curative effect controllability, and an innovative solution is provided for cornea disease treatment.

This invention discloses an injectable hydrogel with piezoelectric effect, its preparation method, and its application. The method includes: under sterile conditions, dissolving medical-grade gelatin at 1-5 w / v% in PBS at pH 7.4, and continuously stirring at a water bath temperature of 40-45°C until completely dissolved; then adding glycerol to obtain a gelatin solution; and incorporating barium titanate at 0.1-1.5% by mass into the gelatin solution and stirring until homogeneous. This invention forms a stable BT-gelatin composite scaffold by uniformly dispersing BT nanoparticles in a gelatin hydrogel precursor solution. The key advantage of this composite system lies in its injectability: it is in a fluid state at room temperature, facilitating precise delivery to the intervertebral disc via a minimally invasive needle; under in vivo body temperature, the gelatin chains rapidly cross-link and solidify, firmly anchoring the BT particles in the gel network, thereby achieving in-situ retention and long-term functionalization of BT within the degenerated nucleus pulposus, providing a practical delivery solution for piezoelectric stimulation repair of the intervertebral disc.

The invention discloses a soybean oil-based composite bone scaffold with photo-thermal mediated shape memory and thermal therapy functions and a preparation method of the soybean oil-based composite bone scaffold. The method comprises the following steps: taking bio-based acrylated epoxidized soybean oil and polyethylene glycol diacrylate as a composite matrix, doping natural melanin nanoparticles as functional components into the composite matrix to prepare composite photosensitive resin, and finally preparing the porous scaffold through a digital light treatment 3D printing technology. Under irradiation of near-infrared light, the composite scaffold shows a controllable and efficient photo-thermal response behavior, and the target steady-state temperature can be achieved by adjusting the loading capacity and irradiation conditions of the natural melanin nanoparticles. On one hand, the photothermal effect can trigger shape memory recovery so as to realize minimally invasive implantation and shape self-adaptive filling of irregular bone defects. On the other hand, the composite scaffold shows an efficient photo-thermal inhibition effect (the inhibition rate exceeds 90%) on human osteosarcoma cells. In addition, the composite scaffold exhibits enhanced in vitro biomineralization ability. The invention provides a promising solution for designing and preparing a multifunctional bio-based 3D printing bone scaffold to cope with the challenge of complex bone defects.

The invention belongs to the technical field of preparation of tissue engineering scaffolds, and discloses a flexible composite scaffold based on digital light printing polycaprolactone and a surface coating and a preparation method of the flexible composite scaffold. The flexible composite scaffold with the surface coating comprises a polycaprolactone skeleton, and a silk fibroin film layer and the surface coating which are sequentially positioned on the skeleton from inside to outside, the surface coating is a modified layer of functional molecules, and the functional molecules simultaneously comprise PEGDA, GelMA and RGDMA. According to the invention, a polycaprolactone three-dimensional skeleton printed by a digital light processing technology is used as a configuration support frame, the outer layer of the polycaprolactone three-dimensional skeleton is coated with a silk fibroin film, and the surface of the silk fibroin film is modified with functional molecules, so that the composite scaffold which is programmable in shape, adjustable in mechanical property and has biological activity is realized through a collaborative construction mode; the method is suitable for various tissue engineering application fields such as bone repair, valve regeneration and soft tissue repair, and has excellent application prospect and popularization value.

The invention relates to a composite bio-ink, a preparation method thereof and a bone organ constructed based on the composite bio-ink. The composite bio-ink can be used for extrusion type 3D bio-printing, and the composite bio-ink adopts methylacryloylated gelatin (GelMA) as a matrix, and aloe gel with M2 polarization capability and nano-clay which endows the material with structural stability are introduced. The composite bio-ink provided by the invention is multifunctional composite bio-ink with an endogenous immunoregulation function and excellent osteogenesis performance, and breaks through the traditional immunoregulation mode depending on short-term exogenous cytokines, so that precise regulation and control of a local immune microenvironment are realized, functional regeneration of bone tissues is promoted, and the osteogenesis effect is improved. Higher biological safety and clinical transformation potential are realized. According to the composite bio-ink, a 3D extrusion type printing forming method is adopted, and a composite scaffold with multiple functions of immune induction, biocompatibility, mechanical strengthening, osteogenesis promotion and the like can be constructed. Based on the composite scaffold, a bone organ can be further prepared.

The invention discloses a composite gel scaffold as well as a preparation method and application thereof. The preparation method of the composite gel scaffold comprises the following steps: dissolving calcium-deficient hydroxyapatite and recombinant collagen in water, adding a cross-linking agent, stirring and standing to prepare the composite gel scaffold; the preparation method of the calcium-deficient hydroxyapatite comprises the following steps: S1, calcining natural hydroxyapatite powder for 2 to 6 hours at the temperature of 700 to 900 DEG C; s2, soaking the calcined hydroxyapatite in a modifier solution to supplement phosphorus element; wherein the modifier is a salt containing a phosphorus element; and S3, drying the soaked hydroxyapatite, calcining at 500-700 DEG C for 0.5-3 hours to reduce the crystallinity, and cooling to prepare the calcium-deficient hydroxyapatite. The composite scaffold not only has the soft elastic function of gel, but also has the rigid supporting performance of particles.

This invention relates to the field of bio-tissue engineering technology, and particularly to an injectable amniotic membrane compound gel, its preparation method, and its application. It addresses the problems of degradation and unadjustable mechanical properties of existing materials, easy displacement and sedimentation of amniotic membrane particles, insufficient cell loading, and short-lasting repair effects. The gel comprises a composite scaffold membrane and an aldehyde-based hyaluronic acid gel in a mass ratio of 1:3 to 10. The membrane is formed by freeze-drying and grinding the composite scaffold. The scaffold comprises a sequentially stacked upper electrospun fiber membrane layer, a decellularized amniotic membrane matrix intermediate layer, and a lower electrospun fiber membrane layer, cross-linked and bonded together. The electrospun fibers in the upper and lower electrospun fiber membrane layers have a core-shell structure. The shell layer raw material components include natural polymer materials, and the core raw material components include synthetic polymer materials. The preparation method includes preparing a decellularized amniotic membrane matrix, constructing a gradient fiber membrane layer through coaxial electrospinning, cross-linking with tannic acid, freeze-drying and grinding, and preparing and compounding the aldehyde-based hyaluronic acid gel.

The application relates to a preparation method of a bacterial cellulose-based multilayer structure functional bionic scaffold, which comprises the following steps: firstly, injecting a biomaterial solution into a silica gel model with a microchannel system to perform freeze drying to obtain a porous biological scaffold; secondly, performing crosslinking treatment on the porous biological scaffold; thirdly, inoculating a culture bacteria solution with a Xylobacter concentration of 10 9 ~ 10 15 individuals / mL on the crosslinking-treated porous biological scaffold, and performing static culture and freeze drying to obtain a bacterial cellulose-based composite scaffold; and finally, adopting a coating solution to coat the bacterial cellulose-based composite scaffold through a microchannel system, and performing demolding treatment to obtain the bacterial cellulose-based multilayer structure functional bionic scaffold. The application provides a functional coating process for the multilayer bionic scaffold by using the shrinkage space of the treated material, the micro-pore channel and the injection method of the patterned drainage network, effectively prevents the penetration of the coating solution, and realizes the perfect solidification of the coating solution on the scaffold.