49 results about "Composite scaffold" patented technology

The invention provides a pourable tumor organoid vascularization model and a construction method thereof, and relates to the technical field of organoid culture. A temperature-sensitive degradable material and a photo-crosslinking modified temperature-sensitive material are used, the composite stent is printed through two channels, a multi-component gel mask partition curing process is combined, the stability of a perfusion pipeline is improved, and a partitioned highly-bionic complex microenvironment is accurately constructed in a macroscopic three-dimensional space; and constructing to obtain the perfusion tumor organ vascularization model. The model is high in bionic degree and stable in structure, can be applied to deepening cell and microenvironment interaction research and tumor-related drug screening, and has a good application prospect.

The invention provides a multi-nozzle 3D printing composite scaffold capable of promoting bone regeneration as well as a preparation method and application thereof, the multi-nozzle 3D printing composite scaffold is prepared by adopting multi-nozzle collaborative printing of a 3D printing system, the multi-nozzle 3D printing composite scaffold comprises a synthetic polymer material printed by a high-temperature nozzle and a hydrogel material printed by a low-temperature nozzle, and microfilament patterns formed by the two materials are alternately arranged. Wherein the synthetic polymer material is prepared from polycaprolactone and magnesium oxide nano particles; the hydrogel material is prepared from methacrylated gelatin, methacrylated gilan gum and double drug loading nano particles. The 3D printing composite scaffold provided by the invention has excellent mechanical properties, the double-drug-loaded nanoparticle sequence released osteogenesis promoting and vascular promoting drug coupling osteogenesis-vascularization promoting effects, released magnesium ions synergistically enhance osteogenesis and vascularization performance, and the 3D printing composite scaffold has a good bone defect repair effect and has application potential in the field of bone tissue repair.

Artificial meniscal scaffolds characterized by a composite of circumferential polymer fiber network and orthogonal polymer fiber network embedded in an arcuate bioresorbable matrix comprised of collagen and hyaluronic acid. The orthogonal polymer fiber network prevents separation of the circumferential polymer fiber networks. The polymer fiber networks convert axial compressive forces on the scaffolds to tensile loads on the circumferential polymer fibers. The composite scaffold can be anchored to bone by novel anchoring components that protect the polymer fibers and ensure immediate securement of the artificial meniscal scaffold to bone.

The disclosed composite scaffold provides a highly porous and flexible structure that substantially maintains its three-dimensional shape under tension and provides mechanical reinforcement of the repair or reconstruction-first via scaffold mechanical properties, and subsequently, through newly regenerated functional tissue as the scaffold is resorbed.

The invention belongs to the technical field of biomedical materials, and discloses an anisotropic nanofiber yarn composite scaffold as well as a preparation method and application thereof. The anisotropic nanofiber yarn composite scaffold is formed by compounding methylacryloyl modified gelatin and a nanofiber yarn scaffold prepared through electrostatic spinning. The anisotropic nanofiber yarn composite scaffold not only has more excellent mechanical properties, but also can provide a more suitable microenvironment for cell adhesion and growth, can significantly improve the remodeling efficiency of an extracellular matrix, and has a wide application prospect in the field of heart valve repair.

The invention discloses a modified silk fibroin artificial blood vessel and a preparation method and application thereof.The modified silk fibroin artificial blood vessel comprises a modified silk fibroin composite scaffold and a polyurethane layer, the polyurethane layer is arranged on the modified silk fibroin composite scaffold through electrostatic spinning, and the polyurethane layer is prepared from polyurethane, the modified silk fibroin composite scaffold is prepared from the following raw materials: modified silk fibroin, arginine-glycine-aspartic acid polypeptide modified polycaprolactone, bacterial cellulose, a growth factor and glutaraldehyde. The modified silk fibroin is in bridge connection with the arginine-glycine-aspartic acid polypeptide modified polycaprolactone and is loaded with the growth factor. The comprehensive biocompatibility of the artificial blood vessel can be improved, thrombosis and intimal hyperplasia are effectively inhibited, and therefore the long-term patency rate of the small-caliber artificial blood vessel is greatly increased.

This invention discloses a magnesium alloy composite scaffold, its preparation method, and its application. The composite scaffold comprises a JDBM magnesium alloy scaffold with a highly interconnected porous structure and a composite hydrogel layer filling and encapsulating it. The hydrogel layer is formed by photocrosslinking and curing of methacrylamide-loaded glucose oxidase. This composite scaffold is a bio-battery-like therapeutic system. GOx catalyzes the biocatalytic oxidation of glucose to hydrogen peroxide, while magnesium alloy acts as an electroactive anode, together forming an adaptive electrochemical cascade reaction system. Through spontaneous oxidation dissolution and short-circuit electrochemical reactions, it rapidly removes hydrogen peroxide into water and oxygen while simultaneously promoting the oxidation of magnesium. 2+ Release. Therefore, this system not only transforms the harmful high-glucose microenvironment of diabetes into a microenvironment conducive to bone regeneration, but also achieves multiple synergistic effects of anti-inflammation, anti-oxidation, and bone promotion through the continuous supply of magnesium ions and the timely removal of H2O2, significantly promoting the repair and regeneration of bone defects under diabetic conditions.

This invention provides a calcium phosphate-polycaprolactone composite scaffold, its preparation method, and its applications. The raw materials for preparing the calcium phosphate-polycaprolactone composite include polycaprolactone, β-tricalcium phosphate, and hydroxyapatite. The resulting composite scaffold possesses functions of promoting immunity and regulating bone regeneration. The preparation method of this invention involves first forming a hydroxyapatite / polycaprolactone composite film, which is then broken down and recombined with β-tricalcium phosphate to form a composite solution. The resulting 3D-printed composite scaffold not only possesses good mechanical strength and biocompatibility but also induces stem cell proliferation and osteogenic differentiation, and induces macrophage regeneration and polarization. It can be used to regulate immune cell responses, induce a bone immune microenvironment conducive to bone formation, thereby enhancing bone regeneration, and can be used for the repair and regeneration of tissue defects.

This invention discloses a composite scaffold for promoting bone regeneration in diabetic patients, its preparation method, and its applications, relating to the field of medical biomaterials technology. The composite scaffold for promoting bone regeneration in diabetic patients comprises: a three-dimensional porous framework; and a bioactive hydrogel layer loaded with naringin. The bioactive hydrogel layer loaded with naringin is coated on the surface and pore walls of the three-dimensional porous framework. The technical solution provided by this invention uses a 3D-printed PLGA / HA porous framework to provide mechanical support and osteoconductivity, and a supramolecular hydrogel coating loaded with naringin to endow it with immunomodulatory and drug sustained-release functions. This scaffold has good hydrophilicity and biocompatibility, and can effectively induce macrophage polarization towards the M2 anti-inflammatory phenotype by activating the JAK-STAT signaling pathway, improving the local inflammatory microenvironment of diabetic bone defects. Simultaneously, it indirectly promotes vascular endothelial cell migration, lumen formation, and osteogenic differentiation of bone marrow mesenchymal stem cells by regulating macrophages.

The application discloses a kind of biomimetic composite scaffold with periosteum-cortical bone-cancellous bone structure and its preparation method and application.The scaffold is assembled by independent periosteum biomimetic scaffold module and independent bone trunk biomimetic module.The periosteum biomimetic scaffold module is cylindrical tube structure hydrogel, loaded with zinc silicate particles and periosteum related cells, with the biological functions of promoting cartilage differentiation, secreting nerve and vascular factors and synthesizing periosteum matrix proteins.The bone trunk biomimetic scaffold module is cylindrical bioceramic, with multiple vertical pipes embedded in the dense layer of outer wall to simulate cortical bone, and with staggered structure inside to simulate cancellous bone.Animal experiments show that the biomimetic composite scaffold can effectively promote the integrated regeneration of bone trunk and periosteum in rabbit radial critical size defect model.Therefore, the application provides a new solution for the biomimetic design and regeneration repair of bone tissue engineering.

This invention discloses an injectable hydrogel with piezoelectric effect, its preparation method, and its application. The method includes: under sterile conditions, dissolving medical-grade gelatin at 1-5 w / v% in PBS at pH 7.4, and continuously stirring at a water bath temperature of 40-45°C until completely dissolved; then adding glycerol to obtain a gelatin solution; and incorporating barium titanate at 0.1-1.5% by mass into the gelatin solution and stirring until homogeneous. This invention forms a stable BT-gelatin composite scaffold by uniformly dispersing BT nanoparticles in a gelatin hydrogel precursor solution. The key advantage of this composite system lies in its injectability: it is in a fluid state at room temperature, facilitating precise delivery to the intervertebral disc via a minimally invasive needle; under in vivo body temperature, the gelatin chains rapidly cross-link and solidify, firmly anchoring the BT particles in the gel network, thereby achieving in-situ retention and long-term functionalization of BT within the degenerated nucleus pulposus, providing a practical delivery solution for piezoelectric stimulation repair of the intervertebral disc.

This invention relates to the field of bio-tissue engineering technology, and particularly to an injectable amniotic membrane compound gel, its preparation method, and its application. It addresses the problems of degradation and unadjustable mechanical properties of existing materials, easy displacement and sedimentation of amniotic membrane particles, insufficient cell loading, and short-lasting repair effects. The gel comprises a composite scaffold membrane and an aldehyde-based hyaluronic acid gel in a mass ratio of 1:3 to 10. The membrane is formed by freeze-drying and grinding the composite scaffold. The scaffold comprises a sequentially stacked upper electrospun fiber membrane layer, a decellularized amniotic membrane matrix intermediate layer, and a lower electrospun fiber membrane layer, cross-linked and bonded together. The electrospun fibers in the upper and lower electrospun fiber membrane layers have a core-shell structure. The shell layer raw material components include natural polymer materials, and the core raw material components include synthetic polymer materials. The preparation method includes preparing a decellularized amniotic membrane matrix, constructing a gradient fiber membrane layer through coaxial electrospinning, cross-linking with tannic acid, freeze-drying and grinding, and preparing and compounding the aldehyde-based hyaluronic acid gel.

The application relates to a preparation method of a bacterial cellulose-based multilayer structure functional bionic scaffold, which comprises the following steps: firstly, injecting a biomaterial solution into a silica gel model with a microchannel system to perform freeze drying to obtain a porous biological scaffold; secondly, performing crosslinking treatment on the porous biological scaffold; thirdly, inoculating a culture bacteria solution with a Xylobacter concentration of 10 9 ~ 10 15 individuals / mL on the crosslinking-treated porous biological scaffold, and performing static culture and freeze drying to obtain a bacterial cellulose-based composite scaffold; and finally, adopting a coating solution to coat the bacterial cellulose-based composite scaffold through a microchannel system, and performing demolding treatment to obtain the bacterial cellulose-based multilayer structure functional bionic scaffold. The application provides a functional coating process for the multilayer bionic scaffold by using the shrinkage space of the treated material, the micro-pore channel and the injection method of the patterned drainage network, effectively prevents the penetration of the coating solution, and realizes the perfect solidification of the coating solution on the scaffold.

According to the multi-component synergistic 3D printing multi-structure long-acting anti-infection bone repair composite scaffold and the preparation method thereof, the method comprises the following steps: preparing a PVA aqueous solution as a slurry adhesive, and mixing HA, beta-TCP, the PVA aqueous solution and glycerol as a printing slurry; 3D printing parameters are set, a model is imported, a printing structure, discharging and wiring speeds are set, and a customized support is obtained through 3D printing; drying and sintering the scaffold to obtain a porous bone scaffold without organic matters; preparing porous microspheres ABX coated PLGA loaded with antibiotics by taking gelatin as a pore-foaming agent; and coating the sintered porous bone scaffold with a gelatin coating, and loading the microspheres on the porous bone scaffold to obtain the drug-loaded composite bone scaffold. The bone repair scaffold disclosed by the invention is combined with an antibiotic local drug delivery system to prevent infectious bone defects, can overcome the defects of systemic drug delivery of antibiotics, realizes controllable release of drugs in the whole process in a bone regeneration period, has good antibacterial performance while repairing bone defects in vivo, and also has relatively good compressive strength and biocompatibility.

The invention relates to the technical field of modern biomedicine, in particular to a method for promoting differentiation of human umbilical cord mesenchymal stem cells based on an EVs composite material, which comprises the following steps: 1, preparing a double-layer composite scaffold; 2, preparation and characterization of EVs: primary cartilage cell culture, enzymatic digestion of cartilage tissue, digestion termination and cell collection, EVs extraction, and EVs characterization identification; 3, loading the EVs; 4, separation, identification and differentiation induction of the hUC-MSCs; and 5, differentiation effect detection. The method has the characteristics that the differentiation efficiency and stability are good, the EVs delivery efficiency is remarkably improved, the biocompatibility and safety are good, the operation is simple and convenient, the cost is controllable, and the application scene is flexible and wide.

The invention provides a 3D printing composite material stent and a preparation method thereof. The 3D printing composite material stent is prepared by combining a 3D printing technology, casting, injection molding and a seepage method. According to the method, the porous structure of the 3D printing material and other materials are combined, the stent with excellent mechanical properties is prepared, the problem that a composite stent cannot be effectively and directly prepared through existing 3D printing is solved, and the technical problem that most 3D printing materials such as titanium alloy and tantalum alloy are not firmly combined with other materials is solved. The preparation method comprises the following steps: designing a porous structure three-dimensional model, and exporting printer recognition format data; performing 3D printing to obtain a porous structure; designing and / or selecting other materials and preparing a mold; the porous structure obtained through 3D printing is relatively fixed in a mold; other materials are poured into the mold, and the materials can flow into pores of the 3D printing porous structure; and curing and / or hardening to obtain the 3D printing composite material stent. The 3D printing composite material support can be used in orthopedic implants, has the advantages of being firm in structure, not prone to damage and good in bone ingrowth effect, can be applied to large-segment bone defects of bearing parts, promotes bone tissue regeneration on the basis of achieving mechanical supporting, and achieves the curative effect of repairing the large-segment bone defects.

Provided herein are composite scaffold biomaterials including two or more scaffold biomaterial subunits, each including a decellularized plant or fungal tissue from which cellular materials and nucleic acids of the tissue are removed, the decellularized plant or fungal tissue having a 3-dimensional porous structure, the two or more scaffold biomaterial subunits being assembled into the composite scaffold biomaterial and held together via gel casting using a hydrogel glue; via complementary interlocking geometry of the two or more scaffold biomaterial subunits; via guided assembly based biolithography (GAB); via chemical cross-linking; or any combinations thereof. Methods for producing such scaffold biomaterials, as well as methods and uses thereof, are also provided.

The present application relates to the field of sludge conditioning, and particularly relates to a method for sludge purification conditioning using composite carbon source, which comprises the following steps: mixing sludge and alkaline solution, and then performing heating treatment to obtain pretreated sludge; then mixing the pretreated sludge with composite carbon source, and performing anaerobic fermentation to obtain treated sludge; adding dehydrating agent to the treated sludge, and then performing filtration to obtain dewatered sludge; performing drying treatment on the dewatered sludge, and then completing sludge purification conditioning; wherein the preparation method of the composite carbon source comprises the following steps: mixing calcium hydroxide suspension and aluminum chloride suspension, and then performing reaction to obtain hydrotalcite; mixing the hydrotalcite, chitosan and solvent, and then performing heating drying to obtain composite scaffold material; mixing the composite scaffold material, sodium alginate, peanut cake powder and wood-rotting fungi, and then performing dispersion to obtain composite solution; mixing the composite solution and calcium chloride solution to obtain composite carbon source. The method has the advantages of high treatment efficiency, low operation cost, less secondary pollution and the like.

The application provides a developmental growth plate layered scaffold and a preparation method and application thereof, and belongs to the technical field of bone tissue engineering materials. The developmental growth plate layered scaffold provided by the application is divided into three layers, the upper layer is a cartilage cell layer, the middle layer is a blood vessel barrier layer, and the lower layer is a bone formation layer. The blood vessel barrier layer can be used as biological glue to bond the upper layer and the lower layer, so that the composite scaffold structure is stable and is not easy to break; and the upper layer contains bone marrow mesenchymal stem cells (BMSCs), and the middle layer and the lower layer are cell-free. Under the in-vitro chondrogenic induction culture condition, the BMSCs in the upper layer can pass through the middle layer and colonize in the lower layer, and in the process, part of the BMSCs can differentiate into cartilage cells. The middle layer containing thrombospondin 1 (TSP1) has specific intervention effect on cell migration, which is manifested as inhibition of the migration of vascular endothelial cells, but has no effect on the migration of BMSCs.

The invention provides a 3D printing double-layer gradient composite scaffold and a preparation method and application thereof, the 3D printing double-layer gradient composite scaffold is prepared by adopting a multi-nozzle collaborative printing technology of a 3D printing system, and the 3D printing double-layer gradient composite scaffold comprises a cartilage repair layer and a subchondral bone repair layer. Wherein the cartilage repair layer and the subchondral bone repair layer are respectively prepared by cooperatively printing a thermoplastic polymer material and a photocurable natural polymer material mixed with cells and double drug-loaded nanoparticles through double nozzles of a 3D printing system. The distance between the printed microwires of the cartilage repair layer is smaller than that between the printed microwires of the subchondral bone repair layer. The double-layer gradient composite scaffold is integrally prepared by using a multi-nozzle collaborative printing technology of a 3D printing system, has good mechanical strength and biocompatibility, and can realize integrated repair of osteochondral defects.

The invention discloses an artificial periosteum and a preparation method thereof. The preparation method comprises the following steps: performing directional freeze drying on a first solution and a second solution containing mineralized collagen to respectively form a first porous layer facing a bone surface side and a second porous layer facing a soft tissue side; forming a composite scaffold layer between the first porous layer and the second porous layer by using a composite material solution of a biodegradable high polymer material and a bioactive material; any one of the first solution, the second solution and the composite material solution is firstly placed in a mold to be formed; the pore sizes of the first porous layer and the second porous layer are gradually reduced from the bone surface side to the soft tissue side; compared with a second porous layer, the first porous layer formed by directional freeze drying has higher pre-freezing temperature and lower cooling rate, cooling amplitude, vacuum degree, heating rate and sublimation rate. Through a parameter control mode of a directional freeze-drying process, a bionic structure of aperture difference and in-layer gradient aperture distribution is realized on the premise of not changing a material system.

The application belongs to the field of biomedical materials and biomedical engineering technology, and discloses a DFO@GMs-pDA / PN composite scaffold for promoting vascularization and osteogenesis, and a preparation method and application thereof. The DFO@GMs-pDA / PN composite scaffold for promoting vascularization and osteogenesis is constructed by using electrospinning, pDA modification and emulsification-extraction technology, and by wrapping DFO into GMs and loading the DFO into a pDA surface modified PN electrospinning film. The most superficial DFO@GMs begin to disintegrate and release DFO after implantation, and the maximum drug release rate is reached after 48 hours, so as to promote the ingrowth of new blood vessels, the recruitment of stem cells and the secretion and presentation of related factors in the early stage of bone regeneration, and to provide an important blood supply basis for bone regeneration.

The invention discloses a 4D printing composite scaffold for repairing irregular bone defects and a preparation method and application of the 4D printing composite scaffold. The scaffold has a negative Poisson's ratio structure, is prepared from a body fluid responsive hydrogel material through 4D printing, and can be spontaneously converted into a target shape matched with a bone defect part from an initial shape in a body fluid environment. According to the 4D printing composite stent for repairing the irregular bone defect, provided by the invention, aiming at the core contradiction that the shape of a traditional 3D printing stent is fixed and the irregular bone defect is complex in form and difficult to accurately prejudge before an operation, accurate matching of the defect form before the operation is not needed by virtue of the synergistic effect of the body fluid responsive material and the negative Poisson's ratio structure; when implanted, the stent enters in a compressed state in a minimally invasive mode, spontaneously deforms under stimulation of body fluid after an operation, and finally is highly attached to the contour of a defect part, the limitation that a traditional stent needs to be polished on site and is difficult to adapt to complex defects is thoroughly eliminated, and fundamental crossing from static geometric matching to dynamic function self-adaption is achieved.

This invention discloses a highly active ceramic-collagen composite bone scaffold and its preparation method, belonging to the field of bone repair material preparation technology. Quaternized ammonium inorganic material inclusions, photosensitive resin, and dispersant are mixed and ball-milled to obtain a mixed slurry. The mixed slurry is then printed to obtain a bioceramic porous scaffold green body. The bioceramic porous scaffold green body is sintered to obtain a bioceramic porous scaffold. The bioceramic porous scaffold is then vacuum-mixed with collagen fiber slurry, followed by post-treatment to obtain a ceramic-collagen composite scaffold. The ceramic-collagen composite scaffold is then impregnated with rhBMP-2 solution to obtain the highly active ceramic-collagen composite bone scaffold. The preparation method of this invention not only solves the mechanical properties of the prepared bone scaffold but also achieves good osteogenic effects.

The application belongs to the technical field of metal powder processing, and discloses a degradable gradient porous Zn@beta-TCP composite material support for bone defect repair and a preparation method.The volume fraction of Zn and beta-TCP in the Zn@beta-TCP composite material of the support is preferably 97 vol.% and 3 vol.%; the internal structure of the support is a gradient porous structure, specifically a three-week minimal surface as a pore topological structure, and the pore diameter linearly transitions from 700±50 mu m to 1100±50 mu m.The preparation method is that a gradient porous support model is designed by computer-aided design, Zn is used as a metal matrix, beta-TCP is used as a reinforcing phase, and a Zn@beta-TCP composite material support with a gradient porous structure is prepared by using a laser powder melting technology.The gradient structure design in the prepared support can provide more space for the adhesion and proliferation of osteoblasts, and is beneficial to the transmission of nutrients and the ingrowth of bone tissue.

The invention discloses a method for producing an active rhBMP9 bone repair material based on a double-transgene bombyx mori silk gland biosynthesis system as well as a product and application of the active rhBMP9 bone repair material, and realizes cooperative expression of complex precursor protein Pro-mhBMP9 and precursor protein invertase hFurin in bombyx mori posterior silk glands. An hBMP9 / hFurin double-gene biosynthesis system capable of completing precursor protein shearing, maturation and functionalized silk material synthesis in vivo is established, the rhBMP9 / CaP / Sericin composite scaffold is further constructed by extracting silk protein of double-gene transformation bombyx mori, and the rhBMP9 / CaP / Sericin composite scaffold shows excellent bone repair and angiogenesis capacities in both the cell level and the animal level, and can be used for preparing a bone repair material. And a solid experimental and theoretical basis is provided for a functional protein-material-tissue regeneration integrated strategy based on a silk gland biosynthesis system.

The invention relates to the technical field of biomedical materials, in particular to a controllable degradable collagen-based composite scaffold and a preparation method thereof. The invention relates to an adjustable and degradable collagen-based composite scaffold, which is prepared from the following raw materials in parts by weight: 10 parts of type I collagen, 2 to 4 parts of glycosaminoglycan consisting of chondroitin sulfate and hyaluronic acid, 1 to 2 parts of chitosan, 1 to 1.5 parts of polyvinyl alcohol and 1 to 15 parts of glycerol, and is a flexible film with the thickness of 0.5 to 1.5 mm, the flexible thin film is of a double-layer flexible thin film structure with a surface compact layer and an inner porous layer, pores are formed in the surface compact layer and the inner porous layer, therapeutic drugs are loaded in the pores, and the therapeutic drugs comprise at least one of hemostatics, hormones, contraceptives, antibiotic drugs or traditional Chinese medicine extracts.

The invention discloses application of a bone cell specific Wnt signal in critical bone defect repair, and relates to the field of medicine. A 3D printed composite scaffold for use in the treatment of critical bone defects, bone nonunion or osteoporosis, which is applied in the form of a 3D printed composite scaffold comprising bio-ink and polycaprolactone PCL, the bio-ink comprising a bone-derived acellular extracellular matrix (dECM) in a drug-activated bone cell Wnt signal. The dECM bio-ink based on drug activated bone cell Wnt signals and the PCL are used for constructing the composite scaffold through 3D printing, the composite scaffold is used for repairing critical bone defects and retaining a microstructure and bioactive components of a natural bone matrix, and osteogenic differentiation, osteoclast generation, angiogenesis and neurogenesis of bone marrow stromal cells BMSCs can be remarkably promoted.

This invention discloses a 3D scaffold for repairing defective lung injury, its construction method, and its applications, belonging to the field of biomedical materials and tissue engineering technology. This invention uses cationic polysaccharide chitosan and polymer γ-polyglutamic acid as raw materials, utilizing their electrostatic interaction to achieve cross-linking without cross-linking agents, and combines this with freeze-drying technology to prepare a three-dimensional spherical γ-PGA / CS composite scaffold. The above composite scaffold is combined with bone marrow stem cell exosomes (MSC-Exos) and type II alveolar epithelial cells (RLE-6TN). By loading exosomes onto the scaffold and implanting lung cells, local enrichment and targeted delivery are achieved, solving the problems of poor targeting of bone marrow stem cell exosomes and easy loss during transplantation of individual cells. This functional scaffold can accelerate lung function recovery by regulating the inflammatory microenvironment at the site of lung injury in SD rats, promoting lung cell regeneration, and reducing secondary lung tissue damage. It provides a novel implantable, minimally invasive treatment for defective lung injury-related diseases, possessing good clinical translational potential and application prospects in organ damage repair.

The application provides a perfusable tumor organoid vascularization model and a construction method thereof, and relates to the technical field of organoid culture. The application uses temperature-sensitive degradable materials and photo-crosslinking modified temperature-sensitive materials, prints a composite scaffold through a double-channel, combines a multi-component gel mask partition solidification process, improves the stability of a perfusion pipeline, accurately constructs a partitioned highly bionic complex microenvironment in a macro three-dimensional space, and constructs a perfusable tumor organoid vascularization model. The model has high bionics, stable structure, can be applied to deepening the research on cell and microenvironment interaction and screening of tumor-related drugs, and has good application prospect.