95results about How to "Moderate degradation rate" patented technology

The invention discloses a three-dimensional porous cartilage extracellular matrix sponge scaffold made of natural cartilage, which can compound cells further to construct tissue engineered cartilage, and can be used for clinically repairing cartilage defects. In the invention, conditions which can fully perform cell extraction and form a porous scaffold matrix are provided by processing cartilage into cartilage microfilaments, and then the cell extraction and solidification and/or strengthening treatment are performed to obtain the three-dimensional porous cartilage extracellular matrix sponge scaffold which is completely decellurized. The antigenicity and cell components are removed in the natural cartilage, and an extracellular matrix component of the cartilage is retained to obtain the scaffold with appropriate pore diameter and porosity, suitable degradation rate, good biocompatibility and certain biomechanical strength. The scaffold has the advantages of broad material sources, low cost, simple and feasible preparation technology, and good repetitiveness; and the scaffold can be widely applied in the field of tissue engineering and has good clinical application prospect.

The invention provides an artificial dura mater spinalis, and a preparation method and a use method thereof. The artificial dura mater spinalis has a double-layer structure and concretely comprises an internal layer and an external layer, the internal layer is an oriented polylactic acid/glycollic acid copolymer nanofiber membrane, the external layer is a non-oriented polylactic acid/glycollic acid copolymer-chitosan nanofiber membrane, and the internal layer and the external layer are connected. The invention belongs to the technical field of the biomedicine. The artificial dura mater spinalis can realize the dura mater spinalis regeneration and prevent epidural scarring and adhesion, and can substantially reduce the postoperative cerebrospinal leakage incidence rate.

The invention relates to a composite biomaterial containing cross-linked hyaluronic acid derivative and hydroxylapatite and a preparation method thereof. The composite biomaterial is characterized by containing the cross-linked hyaluronic acid derivative and the hydroxylapatite. The composite biomaterial can be obtained by uniformly mixing hyaluronic acid or soluble salt of the hyaluronic acid with the hydroxylapatite and then carrying out the cross-linked reaction, or can be obtained by firstly preparing the cross-linked hyaluronic acid derivative and then uniformly mixing the cross-linked hyaluronic acid derivative with the hydroxylapatite. The composite biomaterial has good biological compatibility, has suitable rate of biodegradation, mechanical property, pore diameter and porosity, and can be used as the cytoskeleton to be applied to bone or cartilage tissue engineering.

The invention relates to a biodegradable iron-zinc alloy and a preparation method thereof, and belongs to the technical field of design and manufacture of biomedical iron-zinc alloys. The iron-zinc alloy consists of 80-95% of iron and 5-20% of zinc according to the mass percentage. The preparation method comprises the steps: preparing an iron powder and a zinc powder according to the designed components, carrying out ball milling for more than 20 h at the rotational speed of 240-400 r/min under protection of argon gas, to obtain an iron-zinc alloy powder; then using the iron-zinc alloy powder,and under the protection of argon gas, adopting a selective laser sintering process, controlling the laser power to 100-200 W, controlling the scanning rate to 10-30 mm/s, controlling the sintering temperature to 750-1400 DEG C, controlling the light spot diameter to 80-120 [mu]m, and thus preparing the iron-zinc alloy. The biodegradable iron-zinc alloy is molded by combining mechanical alloyingwith selective laser sintering. The biodegradable iron-zinc alloy has suitable degradation rate, high mechanical strength and good biocompatibility, and has a good application prospect in the field ofbone tissue repair.

The invention provides a degradable metal composite plate for the cranio-maxillofacial bone restoration and a preparation method thereof. The metal composite plate comprises a layer of magnesium-basedor iron-based metal plate and two layers of zinc-based metal plates, wherein the two layers of zinc-based metal plates are respectively compounded onto the two surfaces of the magnesium-based or iron-based metal plate to form a zinc-magnesium-zinc three-layer metal composite plate or a zinc-iron-zinc three-layer metal composite plate. The degradable metal composite plate for the cranio-maxillofacial bone restoration of the structure can solve the problems of poor biocompatibility, undegradability and requirement of secondary operation for taking out of a cranio-maxillofacial bone restorationmaterial in clinics. Through controlling the thickness ratio of the magnesium-based or iron-based metal plate and the zinc-based metal plate, the regulation on the metal composite degradation time canbe achieved, so that the degradation speed can better correspond to the human body restoration speed; the human body restoration speed is accelerated. By the coating lamination rolling preparation method, the zinc-based metal plate is compounded onto the surface of the magnesium-based or iron-based metal plate to form the degradable cranio-maxillofacial bone restoration material; the process treatment method is simple; the control is easy; the manufacturing cost is low.

The invention discloses a method for preparing an HA/collagen/chitosan interpenetrating polymer network bracket. The method comprises the following steps: firstly, forming an HA sol system containing polyvinylpyrrolidone in an alkali liquor control system, aging and washing the HA sol system to obtain granular directional arranged HA sol, mixing the HA sol and blended solution of collagen and chitosan, dispersing the mixture by adopting a high-speed dispersion machine to obtain mixed solution of HA/collagen/chitosan, reducing pressure and removing foam from the mixed solution which is crosslinked by mixed cross linker and chitosan cross linker, injecting the mixed solution into a model, and then carrying out post treatment to obtain the bracket. The bracket is compounded with a matrix in a form of HA sol, and the HA in granular directional arrangement has good dispersion in the collagen/chitosan interpenetrating polymer network matrix and forms effective and stable bond with the matrix so as to realize the effective compound of the HA and the collagen/chitosan matrix on the structure and function. The method also effectively solves the problems of collagen and chitosan materials at the same time. The method has simple process and simple operation, and is easy for popularization and application.

The invention discloses a nano silicon dioxide/polymer material guided bone regeneration composite porous membrane and a preparation method thereof. The thickness of the membrane is 0.1-0.3 mm, the mass ratio of nano-silicon dioxide to a polymer material is 1:(2-4), and the polymer material is one or two of a poly(N-isopropyl acrylamide)-polycaprolactone-polyethylene glycol copolymer, polycaprolactone, polylactic acid, polyglycollide and collagen. The nano silicon dioxide/polymer material guided bone regeneration composite porous membrane is high in mechanical strength, good in biocompatibility, moderate in degradation speed and capable of achieving a good physical barrier effect, quickly growing fibroblasts are prevented from migrating to a wound part, a regeneration space is provided forslowly growing bone tissue, and the guided bone regeneration composite porous membrane has wide application prospects.

The invention discloses a method for preparing a cross-linked collagen/chitosan tissue engineering porous support in one-step freeze-drying mode, which uses natural biological material of collagen and chitosan as raw materials, directly adds collagen/chitosan mixed swelling solutions in a glutaraldehyde solution and prepares the collagen/chitosan tissue engineering porous support through a one-step refrigeration freeze-drying mode. The collagen/chitosan support prepared through the method has the advantages of being controllable in mechanical property, proper in degradation rate and good in biocompatiability. Further, the method only needs a one-step freeze-drying process and effectively solves the problems of support collapsing, micro-structure breaking and the like caused by a traditional preparing method. The method is simple and practical, low in energy consumption, time-saving, material-saving and good in repeatability, and the constructed collagen/chitosan porous support can be widely used in the field of tissue engineering and has good clinical application prospect.

The invention belongs to the technical field of environmental protection, and particularly relates to a technology for degrading perchlorate in water by employing a biodegradable material as a carbon source and a used degradable composite material. The biodegradable composite material is prepared from 10-80 parts of polyhydroxybutyrate (PHB) and 10-70 parts of PLC by cross-linking reaction. By adopting the degradable composite material disclosed by the invention, a micro-pore or filiform texture is correspondingly generated on the particle surface along with decomposition of degradable PHB. Thus, follow-up adhesion and growth of microorganisms and development into a biological membrane on the carrier surface are facilitated, slow release of the carbon source is achieved, the problems of reduction of a metabolic function of perchlorate reducing bacteria and reduction of the pollutant removal effect caused by deficiency of the carbon source can be overcome; and the degradation rate is moderate, and the degradable composite material can provide enough carbon source for metabolism of the perchlorate reducing bacteria and also can ensure a long replacement period of the carbon source. Therefore, the average removal rate and handling capacity can be effectively improved, and the treatment time is shortened.

The invention provides a method for preparing collagen/chitosan three-dimensional network artificial cornea scaffold, comprising: taking acetic acid solution as solvent as well as collagen and chitosan as solute for preparing collagen/chitosan mixed solution; adding 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide and N-hydroxy succinimide mixed crosslinking agent into the mixed solution; after that, adding chitosan crosslinking agent; finally, removing bubble and injecting the obtained mixed solution into a model, putting the model into an ultra low temperature refrigerator to be frozen and transferring into a freezer dryer for freeze drying, and obtaining the collagen/chitosan three-dimensional network artificial cornea scaffold.

The invention relates to a preparation method for a double-layer osteochondral tissue repair scaffold. The preparation method comprises the following steps: preparing a first feed liquid which comprises recombinant collagen, sodium hyaluronate and hydroxyapatite; preparing a second feed liquid which comprises recombinant collagen and sodium hyaluronate; freezing and drying the first feed liquid and the second feed liquid so as to form a gel-like double-layer structure; and adding a cross-linking agent into the gel-like double-layer structure for cross-linking. The invention also relates to thedouble-layer osteochondral tissue repair scaffold. The double-layer osteochondral tissue repair scaffold comprises a first layer and a second layer, wherein the first layer is prepared from raw materials including the recombinant collagen, the sodium hyaluronate and the hydroxyapatite; and the second layer is prepared from raw materials including the recombinant collagen and the sodium hyaluronate. The double-layer osteochondral tissue repair scaffold prepared by using the preparation method provided by the invention has excellent mechanical properties, good biocompatibility and proper degradation rate; and the scaffold material can be reused as a raw material for new osteogenesis after being degraded, so the repair of osteochondral tissues is realized.

The invention discloses a preparation method and applications of an antibacterial and biodegradable zinc alloy anastomosis nail, wherein the anastomosis nail is prepared from a biodegradable biomedical zinc alloy with antibacterial effect. The preparation method comprises the following steps: 1, preparing a wire material with a required size by utilizing a biodegradable zinc alloy with antibacterial effect; 2, preparing an anastomosis nail from the wire material obtained in the step 1; and 3, directly implanting the anastomosis nail obtained in the step 2 into a stapler nail bin, carrying outdisinfection treatment, and packaging to obtain the final application product, or further carrying out coating treatment and/or medicine carrying treatment, implanting into a stapler nail bin, carrying out disinfection treatment, and packaging to obtain the final application product. According to the invention, the preparation method is simple in process, the use is convenient after the prepared anastomosis nail is loaded into a medical stapler, the tightness is suitable, the rapid suture and the simple operation are guaranteed while the advantages of antibacterial property, surgical infectionprevention and the like are achieved, degradation and absorption are achieved, and the product can be widely applied to occasions needing tissue and organ suture in gastrointestinal and thoracic surgeries, vascular suture and other surgeries.

The invention discloses a preparation method of a gel material for dental restoration. The preparation method comprises the following steps of: (1) preparing a double-gel network component; (2) preparing a collagen-based antibacterial gel material component; (3) preparing an auxiliary antibacterial component; (4) preparing a premixed solution; and (5) performing gel forming. The gel material has astrong antibacterial effect; in addition, polyethylene glycol-poly-L-lysine and mineral ions are added in the gel curing process; therefore, the gel has strong acid resistance and good stability in an oral cavity environment; due to the fact that the mineral ions and lone pair electrons on carboxyl and nitrogen atoms in gel molecules form coordination bonds, the solidification time of the gel solution is relatively short; the mechanical strength is good; and meanwhile, the degradation time of the gel is slowed down.

The invention discloses an enhanced silk fibroin artificial jaw scaffold. The enhanced silk fibroin artificial jaw scaffold is prepared from the components in percentage by weight: 88-96% of decalcified dentin granules, 3-9% of silk fibroin and 1-3% of PEG. The enhanced silk fibroin artificial jaw scaffold can be used in bone tissue engineering, cells are combined with scaffold materials, the defects of bone source shortage, many postoperative complications, poor biocompatibility of materials and the like are overcome, so that the face of a patient is restored well and the operation cost is reduced; the domestic silkworm silk fibroin, decalcified cementum and PEG are reasonably compounded, the obtained composite scaffold has high mechanical properties, moderate degradation rate in vivo, directional osteogenic differentiation ability, no acid degradation products, and immune rejection is reduced.

The present invention relates to the technical field of biomedical materials and particularly to a 3D stent for constructing an in-vitro tumor model and a preparation method and an application of the3D stent. The 3D stent mainly comprises the following raw materials in parts by weight: SF, Cs and Alg at a ratio of 1:1:1. The present invention also comprises the application of the 3D stent for constructing the in-vitro tumor model. Advantages are as follows: step 1, by selecting each raw material and the optimal proportion among the raw materials, and preferably selecting the preparation method, the 3D obtained stent is moderate in degradation rate, moderate in expansion rate, high in water absorption and high in porosity; step 2, a 3D in-vitro culture system is used for replacing 2D cellculture for in-vitro cell culture of colon cancer, biological behaviors of the colon cancer cells in vivo are simulated more truly, and a more reliable basic research basis is provided for diagnosis and treatment of the colon cancer; and step 3, the in vitro tumor model constructed by the stent provides simplicity and convenience for basic scientific research institutions and also is close to growth state of in-vivo tumors.

The invention relates to the technical field of 3D printing and particularly relates to a method for preparing a strontium-containing biological composite material based bone bracket. The method comprises the specific steps: (1) preparing a sodium alginate solution; (2) adding biological inorganic material powder into the sodium alginate solution in multiple times, and carrying out homogeneous dispersion, so as to obtain a biological inorganic material-sodium alginate suspension; and (3) subjecting the biological inorganic material-sodium alginate suspension obtained in the step (2) to crosslinking for 1min or more with a solution containing strontium ions while controlling the crosslinking temperature to 4 DEG C to 80 DEG C, thereby obtaining a strontium-containing biological composite material. According to the strontium-containing biological composite material and the preparation method therefor, provided by the invention, the porous strontium-containing biological composite material for bone repair has good biocompatibility, appropriate degradation rate and good osteogenesis induction capability, the clinical requirements of bone-defection regeneration repair materials on performance are met, and the clinical application of the strontium-containing biological composite material is promoted.

The invention belongs to the technical field of medicine and sanitation, and particularly relates to preparation and application of biological adhesion enhanced temperature-sensitive chitosan-based postoperative adhesion prevention hydrogel. The preparation method comprises the following steps: 1, grafting hydroxybutyl groups on chitosan to prepare hydroxybutyl chitosan with temperature-sensitive performance; 2, grafting thioglycollic acid on the chitosan to prepare the thiolated chitosan with enhanced biological adhesion performance. and 3, physically mixing the hydroxybutyl chitosan solution and the sulfhydrylated chitosan solution according to a certain proportion to obtain the biological adhesion enhanced temperature-sensitive chitosan-based postoperative adhesion prevention hydrogel. The product has the following advantages: the product has reversible temperature-sensitive phase change ability, is a liquid with good fluidity at low temperature, is convenient to inject and use, can rapidly flow to fit and cover irregular wounds after being injected to the wounds, rapidly forms a hydrogel barrier at physiological temperature to isolate adjacent tissue or organ surfaces, and prevents postoperative adhesion.