128 results about "Bone tissue engineering" patented technology

The invention discloses a cerium oxide-based nano material for bone defect repair and a preparation method of the cerium oxide-based nano material. The cerium oxide-based nano material for bone defect repair is prepared by performing surface modification on cerium oxide-based nano enzyme, and the surface modification comprises introducing a targeting ligand and / or carbon quantum dots into the cerium oxide-based nano enzyme. The invention also discloses a cerium oxide-based composite bone scaffold. The cerium oxide-based composite bone scaffold is prepared by compounding the cerium oxide-based nano-enzyme and / or the cerium oxide-based nano-material and the bone tissue engineering material.

The invention provides an interface-enhanced regenerated bone scaffold for sustained-release medicines as well as a preparation method and application of the interface-enhanced regenerated bone scaffold, and belongs to the technical field of bone tissue engineering. According to the invention, polycaprolactone and hydroxyapatite are taken as matrix components of the regenerated bone scaffold, so that the regenerated bone scaffold has mechanical strength matched with a host bone and good biocompatibility; the polydopamine coating is loaded on the surface and internal pores of the regenerated bone scaffold matrix, surface functional modification is easy to perform, and a catechol structure in the polydopamine coating can perform coordination binding with metal ions in MOFs, so that the falling rate of MOFs particles is reduced, and the MOFs particles are uniformly and stably distributed; the MOFs particles are used for loading the eucommia ulmoides extract, safety and non-toxicity are achieved, the eucommia ulmoides extract is encapsulated in the MOFs particles, the stability of the eucommia ulmoides extract can be improved, and a better drug release effect and a better bone regeneration promoting effect are achieved.

The invention provides a preparation method of a mineralized collagen scaffold material with a bionic structure, and belongs to the field of biomedical engineering.The preparation method comprises the steps that an amorphous calcium phosphate solution stabilized by polyacrylic acid is prepared, and the molar ratio of Ca to P in the amorphous calcium phosphate solution is 1.67: 1; preparing a collagen solution containing noradrenaline; carrying out isoelectric focusing treatment on the collagen solution under a constant voltage to form a dense membrane, and stretching the dense membrane to generate 120-200% deformation, so as to obtain a collagen membrane with a bionic structure; and soaking the collagen membrane in the amorphous calcium phosphate solution, and then performing freeze drying to obtain the noradrenaline-mineralized collagen scaffold material. The mineralized collagen scaffold material prepared by the method has excellent biocompatibility, osteogenesis inductivity and sufficient mechanical strength, can effectively promote bone tissue defect repair, and has a good application prospect in the field of bone tissue engineering. The invention further provides the mineralized collagen scaffold material with the bionic structure and application of the mineralized collagen scaffold material.

The invention belongs to the technical field of cell small molecule compound reprogramming and bone tissue engineering material culture, and discloses a method for inducing fibroblasts to be reprogrammed into osteoblasts. According to the invention, small molecule compounds act on fibroblasts, each small molecule is endowed with characteristics of a set of characteristic gene expression mode, and the small molecules are divided into different modules according to the expression characteristic mode, so that the fibroblasts of the cells are converted into osteogenic differentiation, the osteogenic differentiation effect is enhanced and maintained, and the in-vivo development of the cells is simulated to a great extent; therefore, healthy osteoblasts can be prepared from the skin fibroblasts, cell stress pressure can be resisted, and the prepared cells are free of tumorigenicity, safe and healthy. The osteoblast disclosed by the invention can be applied to preparation of bone materials and medicines for treating bone defects, for example, the osteoblast and a traditional bone material are used for constructing an active bone tissue engineering material, so that the use of allogeneic bones commonly used in clinic at present is avoided, and the occurrence of adverse ethical events is reduced.

The invention relates to an E7 peptide grafted methacrylamide karaya harlequin tree hydrogel scaffold and a preparation method thereof, the hydrogel scaffold is a comprehensive bone tissue engineering scaffold, the diameter is 140-680 nm, and the hydrogel scaffold includes but is not limited to regeneration and repair of bone defects clinically; the methacrylamide and karaya karaya are mainly used as main base materials and grafted with E7 peptide, and the finally obtained methacrylamide karaya karaya hydrogel scaffold grafted with E7 peptide has a plurality of uniformly distributed pores and has a good intercommunication microstructure. The method is simple and clear in design, the shape and the microstructure of the scaffold can be accurately controlled, the mechanical property can be adjusted according to needs, the cost is relatively low, the method is suitable for tissue repair, and a preliminary research foundation is laid for exploring a novel tissue engineering scaffold.

The invention relates to the technical field of ceramic aerogel, in particular to hydroxyapatite biological ceramic aerogel with a directional hole structure as well as a preparation method and application of the hydroxyapatite biological ceramic aerogel. The preparation method comprises the following steps: firstly, adding hydroxyapatite particles, a sintering aid and a binder into a water solvent according to a certain mass ratio to prepare aerogel ink; and secondly, filling the ink into an extrusion needle tube of a direct-writing 3D printing technology, and carrying out customized printing on hydroxyapatite aerogel on a liquid nitrogen cooled copper plate by adopting an ice template method as an assistance. After freeze drying and sintering, the biological ceramic aerogel with the directional hole structure on the micro scale is finally obtained. The oriented pore biological ceramic aerogel has excellent mechanical properties and biocompatibility, and has good prospects in the fields of biological scaffolds, bone tissue engineering, biosensor substrates and the like. The method for preparing the hydroxyapatite biological ceramic aerogel by using the ice template method to assist the 3D printing technology is low in cost, simple to operate and wide in application range, and has great application potential.

The invention provides porous biological ceramic as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing aluminum oxide slurry with a pore forming agent to obtain mixed slurry; carrying out light curing reaction on the mixed slurry in a layer-by-layer projection manner by adopting DLP 3D printing, and carrying out layer-by-layer printing, accumulation and forming to obtain a ceramic body; according to the preparation method, through the aluminum oxide slurry composite pore-forming agent and the DLP 3D printing technology, it is guaranteed that no through hole exists in the material, high density and mechanical strength are maintained, and the problem that in the prior art, surface performance and mechanical performance are difficult to consider at the same time is solved; a discrete porous structure is constructed on the surface of the high-solid-content aluminum oxide ceramic, so that accurate regulation and control of the surface roughness are realized, surface discrete holes are directly formed, and a complex post-treatment process is omitted; through the sintering process, the overall sintering shrinkage rate is controlled, the forming precision of a complex structure is improved, the large-scale production requirement is met, and the material can be applied to bone tissue engineering scaffolds and tooth restoration materials.

The present invention relates to the technical field of bone tissue engineering scaffolds, and discloses a modified polycaprolactone scaffold and a preparation method thereof. The modified polycaprolactone scaffold comprises the following preparation raw materials: polycaprolactone, bioactive ceramics, solvent I and composite hydrogel; the composite hydrogel comprises a hydrogel solution and ZIF‑8 surface-modified polyester microspheres dispersed therein. The modified polycaprolactone scaffold provided by the present invention has a compressive strength higher than 20MPa, low in vitro cytotoxicity, high biosafety, no risk of hemolysis, can well induce cells to secrete alkaline phosphatase, has osteogenic activity, can also exert a long-term antibacterial effect, and is suitable for the repair and regeneration of bone tissue defects under infection.

The invention relates to the technical field of bone tissue engineering and regenerative medicine, and discloses a stem cell-loaded bionic bone scaffold material, which comprises a bone scaffold main body, the bone scaffold main body is formed by compounding hydroxyapatite and a biodegradable high-molecular polymer and has a porous structure, the porosity is 70%-90%, the aperture range is 100-500 microns, and pores are communicated with one another; a vascularization induction factor slow release system is arranged in the porous structure of the bone scaffold main body, the stem cell-loaded bionic bone scaffold material does not need to take the autologous bone of a patient, and the problems of donor injury and limited sources are avoided; the composite scaffold is matched with low-immunogenicity stem cells, so that the risks of immunological rejection and disease transmission are avoided; the material has the advantages of good biocompatibility, mechanical matching with human bones, degradability, no secondary operation, promotion of vascular regeneration and stem cell osteogenesis, and realization of bone regeneration integration.

The invention provides a developmental growth plate layered stent as well as a preparation method and application thereof, and belongs to the technical field of bone tissue engineering materials. The developmental growth plate layered stent provided by the invention is divided into three layers, the upper layer is a cartilage cell layer, the middle layer is a blood vessel barrier layer, and the lower layer is an ossification layer. The blood vessel barrier layer can be used as biological glue to bond the upper layer and the lower layer, so that the composite stent is stable in structure and not easy to break; the upper layer contains bone marrow mesenchymal stem cells (BMSCs), and the middle layer and the lower layer are free of cells. Under the condition of in-vitro chondrogenesis induction culture, the BMSCs on the upper layer can penetrate through the middle layer and be colonized on the lower layer, and in the process, part of the BMSCs can be differentiated into cartilage cells. The middle layer containing the platelet reaction protein 1 (TSP1) has a specific intervention effect on cell migration, which is expressed by inhibiting the migration of vascular endothelial cells, but has no influence on the migration of the BMSCs.

The invention discloses a high-biological-activity human-derived I-type collagen variant, a composite material based on the high-biological-activity human-derived I-type collagen variant and a preparation method. According to the invention, the COL109 fragment which can be efficiently secreted and expressed in pichia pastoris and has a triple helix structure is obtained through screening; an adhesion domain (GPP) 10 is further fused to improve the mechanical strength, and a variant COL109 (GPP) 10 is obtained; and finally, compounding with polycaprolactone (PCL) according to an optimized ratio (1: 1) to obtain the composite material with high biological activity (promoting osteogenic differentiation and wound healing) and excellent mechanical properties. The material is suitable for bone tissue engineering scaffolds, skin regeneration dressings and the like.

The invention relates to bioglass powder containing nano titanium nitride as well as a preparation method and application of the bioglass powder. The bioglass comprises the following components in percentage by mass: 75-95% of bioglass powder and 5-25% of nano titanium nitride powder, the melting process of the bioglass containing nano titanium nitride comprises the following conditions: the temperature is 650-800 DEG C, and the time is 10-15 minutes; the photo-thermal treatment method comprises the step of applying near-infrared light to the nano titanium nitride-containing bioglass powder to regulate and control release of active ingredients in the bioglass, and can also comprise the step of inducing generation of hydroxyapatite on the surface of the bioglass. Compared with the prior art, the bioglass not only has a remarkable photothermal effect, but also has the advantages that the nano titanium nitride powder is not easy to lose and the photothermal performance of the bioglass containing the nano titanium nitride is kept good in an experiment of simulating implantation into a human body environment; the release of active ingredients in the bioglass and the generation of hydroxyapatite on the surface of the bioglass are regulated and controlled by applying near-infrared light, so that controllable and sustainable repair of bone tissues is realized. The technology can be applied to the fields of bone repair, regeneration and the like in bone tissue engineering.

The invention provides a porous biological ceramic hard tissue slice as well as a preparation method and application thereof. The preparation method of the porous biological ceramic hard tissue slice comprises the following steps: preparing a hydroxyl phospholime bone restoration specimen; dehydrating and transparentizing the hydroxyapatite bone restoration specimen; carrying out permeation and embedding on the hydroxyl phospholime bone restoration specimen; carrying out block trimming and slicing on the hydroxyl phospholime bone restoration specimen; mounting the hydroxyl phospholime bone restoration specimen; through the five steps, the porous biological ceramic hard tissue slice is obtained. According to the method, the defect that a tissue-material structure is easy to damage when a biological ceramic material is treated by a traditional hard tissue slicing technology is overcome; the improved hard tissue slicing technology is suitable for research of biological ceramic materials in bone tissue engineering application.

The invention belongs to the field of biomedical engineering, and particularly relates to gradient collagen hydroxyapatite conductive hydrogel and a preparation method thereof. The preparation method comprises the following steps: uniformly distributing hydroxyapatite in a collagen solution, adding sodium alginate and polyvinyl alcohol, and mixing to obtain a mixed solution; hydroxyapatite particles are distributed in a gradient mode along a hydrogel support through centrifugal treatment, then freezing treatment is conducted, a salt solution composed of metal ions and hydrophilic anions is introduced, on one hand, the introduced metal ions can form a cross-linked structure on sodium alginate, and on the other hand, the hydrophilic anions can form a hydrophilic structure on the other hand; meanwhile, part of freely moving metal ions can be intercepted in the hydrogel to provide conductivity for the gel; on the other hand, the mechanical property of the hydrogel scaffold is rapidly improved by hydrophilic anions through an ion specificity effect. Therefore, the hydrogel has excellent mechanical properties and conductivity, and meanwhile, due to gradient distribution of hydroxyapatite, the hydrogel is more similar to a full-thickness osteochondral tissue structure, and the hydrogel has a better application prospect in bone tissue engineering.

A scaffold for bone-tissue engineering includes a mesh and a coating on the mesh. The mesh includes a plurality of offset layers of filaments of polyester. The coating covers the mesh and includes magnesium hydroxide / copper oxide nanoparticles suspended in gelatin. A method of constructing the scaffold comprises combining magnesium hydroxide / copper oxide nanoparticles with a gelatin solution and coating a polyester mesh with the combined magnesium hydroxide / copper oxide nanoparticle and gelatin solution.

The invention belongs to the field of bone tissue engineering, and particularly relates to a frozen 3D printing stent with synergistic signal molecules and a preparation method and application thereof.The ink composition with the synergistic signal molecules comprises a first porous carrier loaded with CO molecule prodrugs and a second porous carrier loaded with NO molecule prodrugs, the surface of the first porous carrier is coated with a first encapsulation layer, the first encapsulation layer is used for blocking the CO molecule prodrug in pores of the first porous carrier, and the first encapsulation layer can be self-degraded and release CO molecules under the condition of high oxygen activity; the surface of the second porous carrier is coated with a second encapsulation layer, the second encapsulation layer is used for blocking the NO molecule prodrug in pores of the second porous carrier, and the second encapsulation layer can be self-degraded and release NO molecules under an acidic condition. By means of the method, the stent which is complete in structure, capable of achieving timely vascularization and capable of stabilizing osteogenesis can be obtained.

The utility model relates to the technical field of medical instruments, and discloses a composite support for bone tissue engineering, which comprises a shell, a plurality of fixing rings are equidistantly mounted on the outer wall of a supporting layer, a filter screen is mounted in the middle of the outer wall of each fixing ring, and a fixing block is fixedly connected to the bottom end of each fixing ring. A bottom plate is fixedly connected to the bottom end of the fixing block, a connecting pipe is fixedly connected to the middle of the top end of the bottom plate, a plurality of second medicine outlet holes are formed in the top wall of the bottom plate at equal intervals, a second cavity is formed in the middle of the fixing block and the middle of the connecting pipe, and a first cavity is formed in the middle of the shielding layer and the middle of the supporting layer. According to the utility model, the cavity I can be filled with medicines for diminishing inflammation, relieving pain, resisting infection and promoting cartilage growth, the medicines enter the connecting tube and then flow into the adjacent cavity II from the groove, and then the medicines are guided to a fracture area through the medicine outlet II, so that smooth and rapid growth of bone tissue cells can be effectively promoted, and damaged bone tissue can be repaired.

The invention belongs to the technical field of biological materials, and particularly relates to a nano composite material for bone repair as well as a preparation method and application thereof. One of the purposes of the present invention is to provide a nano composite material for bone repair, the nano composite material comprising anthraquinone derivative molecules and metal ions forming a nano network structure, the nano network structure being formed by a carbonyl group and an alpha-phenolic hydroxyl group structure in the anthraquinone derivative molecules on the basis of the coordination of adjacent oxygen atoms to the metal ions. The nanocomposite not only shows good biocompatibility, but also can effectively promote proliferation of rat bone marrow mesenchymal stem cells (rBMSCs) and accelerate the osteogenic differentiation process, thereby having important application value in bone tissue engineering and repair medicine.

The invention belongs to the technical field of biomedical materials and bone tissue engineering, and discloses preparation and application of a self-piezoelectric aluminum nitride film / cavity assembly structure for promoting large-section critical bone defect repair. The thin film is based on the piezoelectric property of AlN, the self-power-generation capacity in the physiological vibration environment is achieved by optimizing the cavity structure, and the surface current density of AlN is remarkably improved. The film provided by the invention can continuously provide stable bio-electricity signals under the conditions of no polarization treatment and no external physical stimulation, so that the adhesion, proliferation and differentiation of osteoblasts are promoted. In-vivo experiments show that the film / cavity assembly structure can effectively repair the critical bone defect of the rabbit femur without a stent, and is superior to an existing piezoelectric material. The limitation that a traditional piezoelectric bone repair material depends on external stimulation and a stent is broken through, and an innovative strategy with high clinical transformation potential is provided for bone tissue repair.

The invention relates to the field of bone tissue engineering and the field of biomedical materials, in particular to application of an ultrasonic response piezoelectric collagen scaffold. According to the invention, full-thickness pigskin is used as a raw material, hair and subcutaneous adipose tissue are removed, and then decellularization is carried out so as to obtain the membrane-like scaffold. The membrane-like scaffold retains a complete three-stage structure of natural collagen, which is a source of the natural collagen having a piezoelectric effect; meanwhile, a piezoelectric signal detection result shows that periodic piezoelectric signals appear on the membrane-shaped stent, and then the collagen stent provided by the invention is proved to have an intrinsic piezoelectric effect. Experimental results show that mesenchymal stem cells can be attached to the surface of the collagen scaffold and can promote repair and regeneration of bone tissues, and in addition, rat subcutaneous ectopic bone regeneration experiments prove that the collagen scaffold provided by the invention has a good bone regeneration promoting effect under ultrasonic stimulation.

The invention provides composite hydrogel, photo-thermal response drug release composite hydrogel as well as a preparation method and application of the photo-thermal response drug release composite hydrogel, and belongs to the technical field of biomedical materials. The hydrogel is constructed by taking methylacryloyl gelatin as a matrix and combining lauric acid microspheres carrying an osteogenesis promoting drug baicalin, a reduced graphene oxide photothermal agent and a photoinitiator LAP. Under the irradiation of near-infrared light, the hydrogel can realize local mild temperature rise, induce the lauric acid microspheres to generate solid-liquid phase change, release the entrapped osteogenesis promoting drug as required, realize the synergistic effect of photothermal therapy and controlled release of the drug, and have a good osteogenesis repair effect. The hydrogel has good mechanical properties, biocompatibility and degradability, can be used for local treatment of bone defect parts, and effectively promotes regeneration and repair of oral and maxillofacial bone tissues. The invention provides a novel controllable and efficient treatment thought for bone tissue engineering and regenerative medicine.

The invention belongs to the field of biological materials, and particularly relates to a starfish skeleton bionic scaffold and a preparation method and application thereof.The preparation method comprises the following steps that firstly, nanofiber of bionic starfish porous soft tissue is prepared through an electrostatic spinning technology; then, mineralizing the nano-fibers in simulated body fluid to obtain nano-fibers of which the surfaces are bionic with starfish bones; and finally, combining the starfish skeleton with chitosan hydrogel to obtain the starfish skeleton bionic scaffold. Compared with the prior art, the problem that in the prior art, hydrogel is insufficient in mechanical strength is solved, the starfish bone bionic scaffold is prepared, the synergistic effect of mechanical adaptation and osteogenesis promotion is achieved, and a new bone tissue engineering strategy is provided for clinical treatment of bone defect repair.

This invention discloses a biodegradable, highly bioactive magnesium bone particle, its preparation method, and its applications, belonging to the field of biomedical materials technology. Using magnesium bone particles as a matrix, an active composite coating layer comprising biodegradable polymer components and inorganic phase components is formed on its surface to construct biodegradable, highly bioactive magnesium bone particles. The biodegradable polymer components are one or more selected from polycaprolactone, polylactic acid, polylactic-co-glycolic acid copolymer, polyhydroxyalkanoates, chitosan, and their derivatives; the inorganic phase is one or more selected from hydroxyapatite, titanium dioxide, calcium phosphate materials, and combinations thereof. The preparation method includes magnesium bone particle matrix preparation, active composite layer slurry preparation, magnesium bone particle coating, and post-treatment. The prepared biodegradable, highly bioactive magnesium bone particles exhibit good degradation regulation performance and excellent bioactivity, and can be used in bone tissue engineering, bone defect repair, and scaffold filling fields.

The invention relates to the field of bone tissue engineering and regenerative medicine, in particular to non-denatured bone protein calcium superfine powder with bone tissue oriented growth and rapid repair effects and a preparation method and application of the non-denatured bone protein calcium superfine powder, and the non-denatured bone protein calcium superfine powder comprises the following components in percentage by weight: 25%-45% of non-denatured type I collagen, 0.005%-0.05% of bone morphogenetic protein-2, 0.001%-0.02% of transforming growth factor-beta1, 0.01%-0.02% of calcium sulfate, 0.01%-0.02% of calcium sulfate, 0.01%-0.02% of calcium The bone tissue repairing agent is prepared from, by weight, 0.001%-0.02% of basic fibroblast growth factors, 20%-40% of ultramicro hydroxyapatite nanoparticles, 5%-15% of chitosan oligosaccharide, 3%-10% of mannitol calcium complex, 2%-8% of sodium hyaluronate, 0.01%-0.1% of vitamin D3, 0.01%-0.1% of vitamin K2, 5%-15% of chondroitin sulfate, 1%-4% of casein phosphopeptides and the balance auxiliary materials, and directional growth and rapid repairing of bone tissue are promoted.

The application belongs to the technical field of bone tissue engineering, and particularly relates to a recombinant collagen-chitosan-PEG-hydroxyapatite bone repair scaffold material, a preparation method and application. The bone repair scaffold material is formed into a one-way ordered three-dimensional scaffold by a directional freezing method through recombinant collagen, chitosan and PEG. Nano-hydroxyapatite is uniformly deposited in the inside and surface through a special mineralization process. The prepared material has a very good bionic natural bone structure and composition, a good reticular structure and mechanical strength, good biocompatibility and cell activity, a significant repair effect on critical size bone defects, a good osteogenic induction effect and bone conductivity. The bone repair scaffold material provides a new potential treatment method for severe bone defects, and has a wide application in the fields of artificial bone, artificial cartilage, biological scaffolds and the like.

The application belongs to the field of biomedical materials and bone tissue engineering technology, and discloses a preparation and application of a self-piezoelectric aluminum nitride film / cavity assembly structure for promoting repair of large segment critical bone defects. The film is based on the piezoelectric properties of AlN, and through optimization of the cavity structure, self-power generation capability under a physiological vibration environment is realized, and the surface current density of AlN is significantly improved. The film can continuously provide stable bioelectric signals without polarization treatment and external physical stimulation, thereby promoting adhesion, proliferation and differentiation of osteoblasts. In vivo experiments show that the film / cavity assembly structure can effectively repair rabbit femoral critical bone defects without a scaffold, and is superior to existing piezoelectric materials. The application breaks through the limitations of traditional piezoelectric bone repair materials which depend on external stimulation and scaffolds, and provides an innovative strategy with high clinical conversion potential for bone tissue repair.

A scaffold for bone-tissue engineering includes a mesh and a coating on the mesh. The mesh includes a plurality of offset layers of filaments of polyester. The coating covers the mesh and includes magnesium hydroxide / copper oxide nanoparticles suspended in gelatin. A method of constructing the scaffold comprises combining magnesium hydroxide / copper oxide nanoparticles with a gelatin solution and coating a polyester mesh with the combined magnesium hydroxide / copper oxide nanoparticle and gelatin solution.

The invention relates to the technical field of biomedical tissue engineering, and particularly discloses a multi-modal response bone tissue engineering material as well as a preparation method and application thereof. The multi-mode response bone tissue engineering material is prepared by mixing methylacryloylated gelatin, sodium hyaluronate subjected to methacrylic acid esterification, 2, 4, 6-trimethylbenzoyl lithium phosphate, Michaene and nano-hydroxyapatite and then carrying out ultraviolet light cross-linking curing on the mixture. In the multi-mode response bone tissue engineering material provided by the invention, MXene has near-infrared light thermal response capability, nHAP provides release of multiple ions such as calcium, phosphorus, magnesium, strontium and the like, vascularization and osteogenic differentiation are synergistically promoted, and GelMA, HAMA and LAP provide mechanical support. Diabetes fracture animal model verification shows that the bone tissue engineering material with multi-mode response provided by the invention can significantly accelerate bone regeneration.

The invention provides a porous tissue engineering scaffold with a drug controlled release function as well as preparation and application of the porous tissue engineering scaffold. The preparation method of the porous tissue engineering scaffold comprises the following steps: S1) preparing double-layer nanoparticles by PLGA-PEG; and S2) preparing a composite scaffold by a low-temperature 3D printing technology. The stent prepared by the method has efficient drug controlled release capability, excellent mechanical properties, good biocompatibility and controllable degradation. The optimized porous structure promotes cell proliferation and tissue regeneration, meanwhile, low-temperature printing keeps the stability and activity of materials and drugs, and the material is widely applied to the fields of bone tissue engineering, local drug delivery and the like and shows remarkable clinical application value and market potential.