55 results about "Bone tissue engineering" patented technology

The invention relates to the technical field of bone tissue engineering and regenerative medicine, and discloses a stem cell-loaded bionic bone scaffold material, which comprises a bone scaffold main body, the bone scaffold main body is formed by compounding hydroxyapatite and a biodegradable high-molecular polymer and has a porous structure, the porosity is 70%-90%, the aperture range is 100-500 microns, and pores are communicated with one another; a vascularization induction factor slow release system is arranged in the porous structure of the bone scaffold main body, the stem cell-loaded bionic bone scaffold material does not need to take the autologous bone of a patient, and the problems of donor injury and limited sources are avoided; the composite scaffold is matched with low-immunogenicity stem cells, so that the risks of immunological rejection and disease transmission are avoided; the material has the advantages of good biocompatibility, mechanical matching with human bones, degradability, no secondary operation, promotion of vascular regeneration and stem cell osteogenesis, and realization of bone regeneration integration.

A scaffold for bone-tissue engineering includes a mesh and a coating on the mesh. The mesh includes a plurality of offset layers of filaments of polyester. The coating covers the mesh and includes magnesium hydroxide / copper oxide nanoparticles suspended in gelatin. A method of constructing the scaffold comprises combining magnesium hydroxide / copper oxide nanoparticles with a gelatin solution and coating a polyester mesh with the combined magnesium hydroxide / copper oxide nanoparticle and gelatin solution.

The utility model relates to the technical field of medical instruments, and discloses a composite support for bone tissue engineering, which comprises a shell, a plurality of fixing rings are equidistantly mounted on the outer wall of a supporting layer, a filter screen is mounted in the middle of the outer wall of each fixing ring, and a fixing block is fixedly connected to the bottom end of each fixing ring. A bottom plate is fixedly connected to the bottom end of the fixing block, a connecting pipe is fixedly connected to the middle of the top end of the bottom plate, a plurality of second medicine outlet holes are formed in the top wall of the bottom plate at equal intervals, a second cavity is formed in the middle of the fixing block and the middle of the connecting pipe, and a first cavity is formed in the middle of the shielding layer and the middle of the supporting layer. According to the utility model, the cavity I can be filled with medicines for diminishing inflammation, relieving pain, resisting infection and promoting cartilage growth, the medicines enter the connecting tube and then flow into the adjacent cavity II from the groove, and then the medicines are guided to a fracture area through the medicine outlet II, so that smooth and rapid growth of bone tissue cells can be effectively promoted, and damaged bone tissue can be repaired.

The invention belongs to the technical field of biological materials, and particularly relates to a nano composite material for bone repair as well as a preparation method and application thereof. One of the purposes of the present invention is to provide a nano composite material for bone repair, the nano composite material comprising anthraquinone derivative molecules and metal ions forming a nano network structure, the nano network structure being formed by a carbonyl group and an alpha-phenolic hydroxyl group structure in the anthraquinone derivative molecules on the basis of the coordination of adjacent oxygen atoms to the metal ions. The nanocomposite not only shows good biocompatibility, but also can effectively promote proliferation of rat bone marrow mesenchymal stem cells (rBMSCs) and accelerate the osteogenic differentiation process, thereby having important application value in bone tissue engineering and repair medicine.

The invention provides composite hydrogel, photo-thermal response drug release composite hydrogel as well as a preparation method and application of the photo-thermal response drug release composite hydrogel, and belongs to the technical field of biomedical materials. The hydrogel is constructed by taking methylacryloyl gelatin as a matrix and combining lauric acid microspheres carrying an osteogenesis promoting drug baicalin, a reduced graphene oxide photothermal agent and a photoinitiator LAP. Under the irradiation of near-infrared light, the hydrogel can realize local mild temperature rise, induce the lauric acid microspheres to generate solid-liquid phase change, release the entrapped osteogenesis promoting drug as required, realize the synergistic effect of photothermal therapy and controlled release of the drug, and have a good osteogenesis repair effect. The hydrogel has good mechanical properties, biocompatibility and degradability, can be used for local treatment of bone defect parts, and effectively promotes regeneration and repair of oral and maxillofacial bone tissues. The invention provides a novel controllable and efficient treatment thought for bone tissue engineering and regenerative medicine.

This invention discloses a biodegradable, highly bioactive magnesium bone particle, its preparation method, and its applications, belonging to the field of biomedical materials technology. Using magnesium bone particles as a matrix, an active composite coating layer comprising biodegradable polymer components and inorganic phase components is formed on its surface to construct biodegradable, highly bioactive magnesium bone particles. The biodegradable polymer components are one or more selected from polycaprolactone, polylactic acid, polylactic-co-glycolic acid copolymer, polyhydroxyalkanoates, chitosan, and their derivatives; the inorganic phase is one or more selected from hydroxyapatite, titanium dioxide, calcium phosphate materials, and combinations thereof. The preparation method includes magnesium bone particle matrix preparation, active composite layer slurry preparation, magnesium bone particle coating, and post-treatment. The prepared biodegradable, highly bioactive magnesium bone particles exhibit good degradation regulation performance and excellent bioactivity, and can be used in bone tissue engineering, bone defect repair, and scaffold filling fields.

The invention relates to the field of bone tissue engineering and regenerative medicine, in particular to non-denatured bone protein calcium superfine powder with bone tissue oriented growth and rapid repair effects and a preparation method and application of the non-denatured bone protein calcium superfine powder, and the non-denatured bone protein calcium superfine powder comprises the following components in percentage by weight: 25%-45% of non-denatured type I collagen, 0.005%-0.05% of bone morphogenetic protein-2, 0.001%-0.02% of transforming growth factor-beta1, 0.01%-0.02% of calcium sulfate, 0.01%-0.02% of calcium sulfate, 0.01%-0.02% of calcium The bone tissue repairing agent is prepared from, by weight, 0.001%-0.02% of basic fibroblast growth factors, 20%-40% of ultramicro hydroxyapatite nanoparticles, 5%-15% of chitosan oligosaccharide, 3%-10% of mannitol calcium complex, 2%-8% of sodium hyaluronate, 0.01%-0.1% of vitamin D3, 0.01%-0.1% of vitamin K2, 5%-15% of chondroitin sulfate, 1%-4% of casein phosphopeptides and the balance auxiliary materials, and directional growth and rapid repairing of bone tissue are promoted.

The application belongs to the technical field of bone tissue engineering, and particularly relates to a recombinant collagen-chitosan-PEG-hydroxyapatite bone repair scaffold material, a preparation method and application. The bone repair scaffold material is formed into a one-way ordered three-dimensional scaffold by a directional freezing method through recombinant collagen, chitosan and PEG. Nano-hydroxyapatite is uniformly deposited in the inside and surface through a special mineralization process. The prepared material has a very good bionic natural bone structure and composition, a good reticular structure and mechanical strength, good biocompatibility and cell activity, a significant repair effect on critical size bone defects, a good osteogenic induction effect and bone conductivity. The bone repair scaffold material provides a new potential treatment method for severe bone defects, and has a wide application in the fields of artificial bone, artificial cartilage, biological scaffolds and the like.

The application belongs to the field of biomedical materials and bone tissue engineering technology, and discloses a preparation and application of a self-piezoelectric aluminum nitride film / cavity assembly structure for promoting repair of large segment critical bone defects. The film is based on the piezoelectric properties of AlN, and through optimization of the cavity structure, self-power generation capability under a physiological vibration environment is realized, and the surface current density of AlN is significantly improved. The film can continuously provide stable bioelectric signals without polarization treatment and external physical stimulation, thereby promoting adhesion, proliferation and differentiation of osteoblasts. In vivo experiments show that the film / cavity assembly structure can effectively repair rabbit femoral critical bone defects without a scaffold, and is superior to existing piezoelectric materials. The application breaks through the limitations of traditional piezoelectric bone repair materials which depend on external stimulation and scaffolds, and provides an innovative strategy with high clinical conversion potential for bone tissue repair.

The invention provides a porous tissue engineering scaffold with a drug controlled release function as well as preparation and application of the porous tissue engineering scaffold. The preparation method of the porous tissue engineering scaffold comprises the following steps: S1) preparing double-layer nanoparticles by PLGA-PEG; and S2) preparing a composite scaffold by a low-temperature 3D printing technology. The stent prepared by the method has efficient drug controlled release capability, excellent mechanical properties, good biocompatibility and controllable degradation. The optimized porous structure promotes cell proliferation and tissue regeneration, meanwhile, low-temperature printing keeps the stability and activity of materials and drugs, and the material is widely applied to the fields of bone tissue engineering, local drug delivery and the like and shows remarkable clinical application value and market potential.

This invention belongs to the field of bone tissue engineering, specifically relating to a 3D printing bio-ink for bone defect repair, its preparation method, and its application. The bio-ink comprises an organic phase and an inorganic phase. The inorganic phase includes osteoinducible calcium phosphate modified with at least one of tannic acid, gelatin, chitosan, and silk fibroin. The organic phase is selected from at least one of sodium alginate, polylactic acid, polyvinyl alcohol, polycaprolactone, and polyetheretherketone. The bio-ink of this invention can better promote bone repair by eliminating foreign body reactions.

The application discloses a kind of biomimetic composite scaffold with periosteum-cortical bone-cancellous bone structure and its preparation method and application.The scaffold is assembled by independent periosteum biomimetic scaffold module and independent bone trunk biomimetic module.The periosteum biomimetic scaffold module is cylindrical tube structure hydrogel, loaded with zinc silicate particles and periosteum related cells, with the biological functions of promoting cartilage differentiation, secreting nerve and vascular factors and synthesizing periosteum matrix proteins.The bone trunk biomimetic scaffold module is cylindrical bioceramic, with multiple vertical pipes embedded in the dense layer of outer wall to simulate cortical bone, and with staggered structure inside to simulate cancellous bone.Animal experiments show that the biomimetic composite scaffold can effectively promote the integrated regeneration of bone trunk and periosteum in rabbit radial critical size defect model.Therefore, the application provides a new solution for the biomimetic design and regeneration repair of bone tissue engineering.

According to the graphene-based stem cell electrical stimulation differentiation device provided by the invention, a flexible graphene electrode array is adopted to release micro-current, an in-vivo bioelectric environment is simulated, and directional differentiation of stem cells to osteoblasts is promoted. The device combines electrical stimulation and impedance monitoring functions, can feed back the cell state in real time, and improves the differentiation efficiency. The graphene electrode has excellent biocompatibility, high conductivity and flexibility, and is suitable for long-term cell culture. By integrating the flexible graphene electrode, micro-current stimulation and impedance monitoring technologies, the invention provides an efficient and controllable stem cell differentiation scheme which can be applied to the fields of bone tissue engineering, regenerative medicine, drug screening and the like.

The invention discloses a porous diamond reinforced polylactic acid composite bone scaffold and a preparation method thereof, and belongs to the technical field of biomedical implants. The stent is integrally formed through a selective laser sintering technology and is composed of a polylactic acid matrix and porous diamonds uniformly dispersed in the polylactic acid matrix, and the mass fraction of the porous diamonds is 2%-16%. The surface of the porous diamond is rich in porous structures and oxygen-containing functional groups, the porous diamond and a polylactic acid matrix form a molecular bonding and mechanical interlocking synergistic enhancement interface, and the mechanical property of the polylactic acid composite bone scaffold is remarkably improved. The preparation method comprises the following steps: (1) weighing polylactic acid powder and porous diamond powder according to a set proportion, and uniformly mixing through vacuum ball milling to obtain composite powder; and (2) performing selective laser sintering on the composite powder in an argon protective atmosphere, and integrally forming to obtain the composite bone scaffold. The problem that traditional diamond particle reinforced phase interface bonding is weak is solved, and the method has wide application prospects in the field of bone tissue engineering.

The invention relates to the technical field of crossing of bone tissue engineering and intelligent antibacterial materials, in particular to a heterojunction nanocomposite and a preparation method thereof, and a heterojunction stent and a preparation method and application thereof. The preparation method comprises the following steps: by taking pCu-BTC and MoS2 as raw materials, carrying out ball milling in a dispersion medium at room temperature, then carrying out intermittent ultrasonic treatment under an ice bath condition to form a type II heterojunction interface so as to obtain a heterojunction nano composite material, compounding the heterojunction nano composite material with poly-L-lactic acid, and then carrying out high-pressure homogenization-3D printing forming, thereby obtaining the ultrasonic response type MoS2 / pCu-BTC / PLLA heterojunction scaffold. The stent has the sonodynamic / chemical dynamic dual-mode antibacterial and osteogenesis promoting functions, is good in biocompatibility and controllable in degradation, can be widely applied to the field of infectious bone defect repair, and provides a novel material solution for antibacterial-bone regeneration synergistic treatment.

The present application relates to the technical field of biomaterials and regenerative medicine, and particularly relates to a preparation method of a whole-fish-scale-based bone repair material, comprising the following steps: S1, fish scale fiber preparation: collected fish scales are washed and subjected to decellularization treatment, and then ground into fibrous form; S2, fish scale collagen adhesive preparation: collagen is extracted from the decellularized fish scales by using at least two different extraction methods, the obtained collagen products are mixed, freeze-dried, and then dissolved in deionized water to prepare collagen adhesives with different concentrations; S3, composite material preparation: the collagen adhesive prepared in step S2 is mixed with the fish scale fibers obtained in step S1, and then extruded into a shape; the whole-fish-scale-based bone tissue engineering material can be prepared by combining fish scale collagen and fish scale fibers, the material is not only safer and more reliable in composition, but also allows accurate regulation of the composition, thereby providing more innovative possibilities for the application of fish scale collagen adhesive.

This invention belongs to the field of bone tissue engineering, and particularly relates to a 3D-printed scaffold with improved bioactivity and high strength, and its preparation method. The method includes the following steps: 3D printing a bio-ink to form a scaffold; the bio-ink comprises an organic phase and an inorganic phase, the inorganic phase comprising osteoinductive calcium phosphate modified with at least one of tannic acid, gelatin, quaternized chitosan, and silk fibroin; the organic phase is selected from at least one of sodium alginate, polylactic acid, polyvinyl alcohol, polycaprolactone, and polyetheretherketone; the mass ratio of the inorganic phase to the organic phase is 1:(0.5-2). The scaffold prepared by this invention exhibits high strength and bioactivity.

The application belongs to the technical field of material chemistry, and discloses a kind of trans-aromatic fumarate copolymer and its preparation method and application, the trans-aromatic fumarate copolymer has the structure general formula shown in formula (I), and the number average molecular weight is greater than 8000 g / mol;Its preparation method is with phthalic anhydride, 1,8-naphthalene dicarboxylic anhydride or glutamic anhydride and maleic anhydride, propylene oxide is raw material, under the catalysis of 2,6-di-tert-butyl-4-methyl phenol magnesium, with benzyl alcohol as initiator, carry out ring-opening copolymerization reaction to form cis-aromatic fumarate copolymer, then isomerization is obtained, because main chain contains aromatic rigid unit, with high molecular weight, stable structure, high photocuring activity, good mechanical property, biodegradable and other advantages, can be prepared suitable for biomedical field biodegradable photocuring 3D printing photosensitive resin, for bone tissue engineering, personalized implant body and other fields, has good application prospect.

The invention relates to a method for three-dimensionally printing bone organs and application, belongs to the technical field of three-dimensional printing, and provides a material composition for three-dimensionally printing the bone organs, the material composition comprises bio-ink, osteoblasts and osteoclasts, and the number ratio of the osteoblasts to the osteoclasts is 1: (2-3.5). Meanwhile, the invention provides a method for carrying out three-dimensional printing on a bone organoid by utilizing the material composition and a method for preparing a bone aging organoid. The bone organoid provided by the invention provides an ideal experimental platform for pathogenesis research of degenerative bone diseases, can be used for bone tissue engineering research, can also provide an important experimental basis for drug screening, treatment strategy optimization and the like of degenerative diseases such as osteoarthritis and the like, and has remarkable scientific value and application potential.

According to the nano bionic bone tissue engineering hydrogel and the preparation process and application thereof, through the anti-oxidation and anti-inflammatory dual-function characteristics of tannic acid (TA), the osteogenesis promoting synergistic effect of TA and CaP / Mg < 2 + > and form-mechanical regulation and control of Gel-MA hydrogel (methylacryloylated gelatin hydrogel), the nano bionic bone tissue engineering hydrogel is prepared; the technical bottlenecks of insufficient inflammation microenvironment regulation, low bone regeneration induction efficiency and incomplete defect repair in the prior art are systematically solved, and an innovative solution with functional suitability and biocompatibility is provided for periodontitis alveolar bone absorption treatment. The nano bionic bone tissue engineering hydrogel can be directly used or used for loading medicines, the nano bionic bone tissue engineering hydrogel is filled into a bone defect area before photocuring (in a liquid state), and then the filled material is subjected to photocuring crosslinking to form a three-dimensional network gel structure which is accurately matched with the shape of the bone defect area.

The application belongs to the technical field of biomedical materials and bone tissue engineering, and particularly relates to a preparation method of a blood-derived GeLMA-lignin biomimetic hydrogel scaffold. The method comprises the following steps: S1: preparing a GeLMA hydrogel precursor; S2: preparing lignin nanoparticles; S3: preparing PRF powder; S4: preparing LIG / PRF nanoparticles; and S5: preparing the blood-derived GeLMA-lignin biomimetic hydrogel scaffold. The single cold source ice mold technology of the application can control the pore direction, and the structure is similar to cancellous bone in height. The lignin nanoparticles realize the triple functions of mechanical enhancement, antioxidant protection and growth factor release. The PRF is derived from autologous blood, has the best physiological proportion and synergistic effect, and avoids the proportion imbalance and immune rejection possibly caused by the addition of exogenous factors. The single cold source process has strong controllability and has industrialization potential.

The application discloses a preparation method of a bone formation inducing biological additive based on red blood cell-derived apoptotic vesicles and application thereof, and the preparation method comprises the following steps: a, red blood cell apoptosis induction treatment; b, collection and purification of red blood cell-derived apoptotic vesicles RBC-ApoEVs through series differential ultracentrifugation; c, characterization and quality control of the apoptotic vesicles; d, acquisition, culture and basic identification of SHED; and e, determination of 0.5 mu g / mL as the optimal bone formation inducing synergistic concentration of the red blood cell-derived apoptotic vesicles RBC-ApoEVs. The bone formation inducing biological additive prepared by the preparation method can be used as a safe and efficient bone formation inducing synergistic agent, and can solve the problem that "proliferation promotion" and "differentiation promotion" are difficult to be coordinated, and can also avoid the dependence on exogenous growth factors and related risks. The bone formation inducing biological additive can be applied to in-vitro induction of SHED bone differentiation, bone regeneration cell suspension and bone tissue engineering compound.

The application discloses a method for preparing wolf tooth rod-shaped calcium phosphate based on ion coordination regulation and directional nucleation, and the method uses calcium chloride as a calcium source, di-ammonium hydrogen phosphate as a phosphorus source, and polyethylene glycol-6000 as a morphology regulator; through a hydrothermal reaction combined with a high-temperature calcination process, wolf tooth rod-shaped calcium phosphate with high monomodal preferential orientation is prepared; EDS energy spectrum scanning proves that the product is calcium phosphate and has excellent biocompatibility, and can be widely applied to bone tissue engineering scaffolds, drug carriers, biological coatings and other fields; the process steps are simple, the reaction conditions are controllable, and the cost is low, so that the application provides a new idea for preparation of high-performance calcium phosphate materials.

The present application relates to the field of bone tissue engineering materials, and relates to a synthetic bone repair material with a biomimetic structure.The present application prepares a nano wool protein / hydroxyapatite composite biomimetic bone material through an in-situ mineralization process, and the wool material is dissolved, purified, dialyzed and separated and purified to obtain a wool protein solution.The wool protein solution is mixed with a calcium ion solution, and a proper surfactant, a pH value regulator and a performance control substance are added to obtain a precursor solution.On this basis, the in-situ mineralization reaction is carried out by dropping the phosphate to obtain the nano wool protein / hydroxyapatite composite biomimetic bone material.The composite biomimetic bone material obtained by the in-situ mineralization deposition has high rigidity and toughness, can simulate the organic / inorganic components in human bones, and has good bone replacement performance.

The invention provides a preparation method and application of a conductive hydrogel composite film, and belongs to the technical field of composite materials. The conductive hydrogel composite membrane is obtained by repeatedly freezing and thawing polyvinyl alcohol (PVA), pectin (Pectin) and MXene and crosslinking with CaCl2 to form a three-dimensional network, the MXene is introduced into a PVA and Pectin hydrogel system, the hydrogel composite membrane is endowed with good mechanical performance, biocompatibility and osteogenesis performance, the hydrogel composite membrane has excellent swelling degradability and conductivity, and the hydrogel composite membrane can be applied to the field of biomedical materials. And the hydrogel composite membrane provided by the invention is non-toxic and has good chemical stability. The hydrogel composite membrane disclosed by the invention can promote bone tissue healing, remarkably enhance the expression of osteogenic factors, promote formation of new blood vessels, promote formation of the blood vessels into bones and strengthen nutrient substance delivery, can further accelerate bone tissue healing in combination with electrical stimulation, and shows a wide application prospect in the field of bone tissue engineering.

The invention relates to a culture medium system capable of efficiently preparing mineralized stem cell spheres, a method for preparing the stem cell spheres by using the culture medium system, the mineralized stem cell spheres prepared by the method and application of the culture medium system or the mineralized stem cell spheres in construction of bone organs, preparation of bone defect repair materials and / or preparation of in-vitro research models. When the culture medium system is used for preparing the mineralized stem cell spheres, the preparation efficiency is high, and the shortest preparation period is only 4 days; and the prepared mineralized stem cell spheres have strong osteogenic ability, show excellent bone repair and osteogenic functions when being applied to preparation of bone repair materials, and provide a new technical path for research and development of efficient and high-performance repair materials in the field of bone tissue engineering.

The application provides a developmental growth plate layered scaffold and a preparation method and application thereof, and belongs to the technical field of bone tissue engineering materials. The developmental growth plate layered scaffold provided by the application is divided into three layers, the upper layer is a cartilage cell layer, the middle layer is a blood vessel barrier layer, and the lower layer is a bone formation layer. The blood vessel barrier layer can be used as biological glue to bond the upper layer and the lower layer, so that the composite scaffold structure is stable and is not easy to break; and the upper layer contains bone marrow mesenchymal stem cells (BMSCs), and the middle layer and the lower layer are cell-free. Under the in-vitro chondrogenic induction culture condition, the BMSCs in the upper layer can pass through the middle layer and colonize in the lower layer, and in the process, part of the BMSCs can differentiate into cartilage cells. The middle layer containing thrombospondin 1 (TSP1) has specific intervention effect on cell migration, which is manifested as inhibition of the migration of vascular endothelial cells, but has no effect on the migration of BMSCs.

The present application relates to the technical field of bone tissue engineering scaffold, in particular to a porous bone scaffold with controllable drug release, a preparation method and application thereof, the porous bone scaffold comprises a hydrogel matrix, a temperature-sensitive drug-loaded microsphere and a photo-thermal conversion agent in the hydrogel matrix, the temperature-sensitive drug-loaded microsphere contains a first active drug, the hydrogel matrix carries a second active drug, the hydrogel matrix has a porous structure, the photo-thermal conversion agent in the hydrogel matrix generates heat under near-infrared light irradiation to trigger the temperature-sensitive drug-loaded microsphere to release the first active drug, and the second active drug in the porous bone scaffold is continuously released. The porous bone scaffold with controllable drug release is used, the drug release rate of the temperature-sensitive drug-loaded microsphere is better than the matrix degradation rate of the hydrogel matrix, the time sequence release of early on-demand release of the first active drug and long-acting sustained release of the second active drug is formed, early angiogenesis and long-acting bone defect repair are promoted, and the overall treatment effect and economy of the porous bone scaffold in complex bone tissue repair are greatly improved.

The invention belongs to the technical field of biomedical materials, and particularly relates to injectable-oxygen-carrying-antibacterial composite hydrogel as well as a preparation method and application thereof. The method comprises the following steps: dissolving dopamine hydrochloride in a buffer solution, adding bioactive glass, carrying out ultrasonic treatment to obtain BGs (at) PDA, dispersing the BGs (at) PDA in water, dropwise adding perfluorooctanoyl chloride, and carrying out ultrasonic treatment to obtain fluorinated bioactive glass; the preparation method comprises the following steps: dissolving hyaluronic acid in water, adding sodium periodate, reacting in a dark place, adding ethylene glycol, continuously reacting, dialyzing and freeze-drying to obtain an oxidized hyaluronic acid solid; and dispersing the fluorinated bioactive glass into a gelatin solution, and then uniformly mixing with an oxidized hyaluronic acid solution, thereby obtaining the product. The hydrogel provided by the invention has injectability, excellent mechanical property and oxygen carrying effect, has good antibacterial effect and biocompatibility, can effectively promote bone defect repair, and has wide application prospect in the field of bone tissue engineering.

The present application relates to a double-nanoparticle co-stable 3D printing Pickering emulsion ink, a preparation method and an application, wherein trace functional element hydroxyapatite (X-HA) and gelatin nanoparticles (GNPs) are used as stabilizers, a mixed solution composed of distilled water, gelatin molecules and a crosslinking agent is used as a continuous phase, n-hexane is used as a dispersed phase, a Pickering emulsion ink is obtained after homogenization emulsification, a porous scaffold material is obtained through a direct ink writing technology of 3D printing, the porous scaffold material is soaked in salt solutions with different concentrations to improve mechanical properties, the dispersed phase is removed, and a hierarchical interconnected porous scaffold is obtained through freeze-drying.The 3D printing Pickering emulsion ink constructed by the present application has excellent rheological properties, can stably flow through a printer nozzle and allow an extruded filament to be immediately deposited.The printed product is stable, has a variety of channel structures, has good biocompatibility, and can be applied to the field of promoting bone repair in bone tissue engineering.