Method for preparing nano hydroxyapatite/polylactic acid composite microspheres

A technology of nano-hydroxyapatite and hydroxyapatite, which is applied in the direction of microsphere preparation and microcapsule preparation, can solve the problem that does not involve the preparation method of nano-hydroxyapatite and polylactic acid composite microspheres, is not suitable for promotion, and is not easy Operation and other problems, to achieve the effect of easy control of the size of the microspheres, good combination of the two phases, and easy operation

Inactive Publication Date: 2009-12-02
CHONGQING UNIV OF ARTS & SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0013] The above-mentioned materials only relate to the preparation of microspheres of polylactic acid and other organic substances, or single polylactic acid, but do not involve the preparation method of nano-hydroxyapatit...

Method used

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  • Method for preparing nano hydroxyapatite/polylactic acid composite microspheres
  • Method for preparing nano hydroxyapatite/polylactic acid composite microspheres
  • Method for preparing nano hydroxyapatite/polylactic acid composite microspheres

Examples

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Embodiment 1

[0029]Weigh 25g of micron hydroxyapatite and place it in four agate grinding jars in an agate ball mill, add large, medium and small agate balls in a ratio of 5:10:20 to each jar, and add grinding agent anhydrous ethanol 10. Milliliter, grind for 7 hours, pour out the ground nano-hydroxyapatite and place it in a far-infrared drying oven to dry at a low temperature of 35° C. to remove the abrasive to obtain nano-scale hydroxyapatite for use. Weigh 6.572g of polylactic acid into an iodine volumetric flask containing 20ml of chloroform, oscillate in an ultrasonic cleaner, and dissolve the polylactic acid in chloroform under the action of ultrasonic waves to prepare a concentration of 0.3286g / ml, then Add 0.3290g (5:100 mass ratio) of nano-hydroxyapatite, continue to ultrasonically oscillate for 2 to 3 hours, keep the ultrasonic water temperature not more than 30 °C, and shake the iodine volumetric flask by hand twice during this period, until the solution is milky white and free o...

Embodiment 2

[0031] Weigh 2.064g of polylactic acid and add it to the iodine measuring bottle filled with 10ml of tetrahydrofuran, vibrate in an ultrasonic cleaner, dissolve the polylactic acid in tetrahydrofuran under the action of ultrasonic waves to prepare a concentration of 0.2064g / ml, then add 0.3102g ( 15:100 mass ratio) according to the nano-hydroxyapatite prepared in Example 1, continue to ultrasonically vibrate for 2 to 3 hours, keep the ultrasonic water temperature no more than 30 ° C, and shake the iodine volumetric flask by hand 3 times during this period, until the solution is milky white and has no Nano-hydroxyapatite particles are precipitated. Take a 1000ml suction filter bottle, add 700ml of water and 0.6102g of polyacryl alcohol, place it on a magnetic stirrer, stir and dissolve at a rotational speed of 1000r / min. Set the diameter of the computer constant-flow pump: 3mm, preset: 10ml, turn: forward, set the flow rate to 5ml / min, and add the mixed nano-hydroxyapatite / poly...

Embodiment 3

[0033] Weigh 0.8041g of polylactic acid and add it to an iodine volumetric flask containing 10ml of dichloromethane, oscillate in an ultrasonic cleaner, dissolve polylactic acid in dichloromethane under the action of ultrasonic waves to prepare a concentration of 0.0804g / ml, and then Add 0.0412g (5:100 mass ratio) of the nano-hydroxyapatite prepared according to Example 1, continue to ultrasonically vibrate for 2-3h, keep the ultrasonic water temperature no more than 30°C, and shake the iodine volumetric flask by hand twice during this period. The solution was milky white and no nano-hydroxyapatite particles were precipitated. Take a 1000ml suction filter bottle, add 700ml of water and 3ml of soil temperature -80, place it on a magnetic stirrer, stir and dissolve at a speed of 800r / min. Set the diameter of the computer constant-flow pump: 3mm, preset: 10ml, turn: forward, set the flow rate to 5ml / min, and add the mixed nano-hydroxyapatite / polylactic acid mixture into the sucti...

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Abstract

The invention discloses a method for preparing nano hydroxyapatite/polylactic acid composite microspheres, which uses nano hydroxyapatite (n-HA) and polylactic acid (PLA) as raw materials and adopts an ultrasonic blending composite process and an emulsification-solvent evaporation method to prepare the microspheres of a nano hydroxyapatite/polylactic acid composite material. The preparation method comprises the following steps: nano-crystallizing hydroxyapatite, proportionally preparing complex liquid, performing ultrasonic dissolving and blending, emulsifying the mixture into small liquid drops, reducing pressure to volatilize a solvent, performing refrigeration and filtration, performing washing, and performing freeze-drying to obtain the composite microspheres. Compared with like products, the composite microspheres have the characteristics that the method has simple and easy operation, the size of the microspheres is easy to control, and the prepared microspheres have large surface holes and specific area, and good mechanical property. The prepared nano hydroxyapatite/polylactic acid composite microspheres are mainly applied to microsphere bonding type brackets in bone tissue engineering and cell micro-carriers in cell engineering, and can be applied to conveying medicaments and bioactive molecules.

Description

technical field [0001] The invention relates to an artificial composite material, in particular, to a preparation method of nano-hydroxyapatite / polylactic acid composite microspheres. Background technique [0002] Polylactic acid (PLA) has been widely used in the preparation of tissue engineering scaffolds due to its good biodegradability, biocompatibility and processability, and is one of the most commonly used bioscaffold materials in tissue engineering research. At the same time, hydroxyapatite (HA) has a similar inorganic composition to human bone tissue, and is an ideal matrix environment for bone cells. The combination with polylactic acid can improve the mechanical properties of polylactic acid and the characteristics of guiding osteogenesis, and it has a high resistance to hydroxyapatite. The excessively rapid degradation of limestone has a controlling effect, which can ensure that the recovery speed of bone tissue is consistent with the degradation speed of the mate...

Claims

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Application Information

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IPC IPC(8): B01J13/02
Inventor 徐文峰廖晓玲张进
Owner CHONGQING UNIV OF ARTS & SCI
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