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Silk fibrin and hydroxyapatite compound material and preparation process thereof

A technology of silk fibroin hydroxyapatite and hydroxyapatite, which is applied in the field of biomedical materials, can solve the problems of limited application area, unsuitable cell culture scaffold, and inability to realize cell culture, etc., to improve biological activity and increase biological activity Effect

Inactive Publication Date: 2006-02-22
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Before the present invention was made, the Chinese patent "Hydroxyapatite Skull Implant" (publication number CN1370507A) disclosed a technology of layering hydroxyapatite powder between silk mesh and hollow nylon mesh to make a skull implant , it adopts the scheme that the layers are connected by adhesives, and then pressurized and heated at about 100°C to form the artificial skull repair material with a composite layer structure due to manual coating or mechanical molding. There are no interpenetrating voids, so this hydroxyapatite composite is not suitable for use as a cell culture scaffold in tissue engineering
Similarly, Hu Jiashan et al. [Material Science and Engineering. 2001, 19(3): 79-83] made carbon fiber and raw silk surface treated with organic reagents to make fibers with a length of about 2mm and mix them with hydroxyapatite. And press molding with 10MPa pressure, the composite bone cement obtained by using the above technology can not form interpenetrating gaps of a certain size, and cannot realize cell culture, so its application is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Add 0.6g of hydroxyapatite (HA) into 20ml of 3% silk fibroin (SF) solution, stir for 10min, place it in ultrasonic wave for homogenization, then add pre-degummed and cut into 5mm long Silk fiber 0.04g, stirred for 30min.

[0025] The above mixture is poured into the metal mold that has been formed, and it is placed in an ethanol bath at -80 ° C for 1 hour at low temperature and quickly frozen, so that the mixture in the mold forms a frozen body.

[0026] Take out the metal mold containing the frozen body, put it in the freezer at -10°C for 20 hours, then immerse the whole mold in an appropriate amount of ethanol solution, let the ethanol slowly penetrate into the silk fibroin hydroxyapatite frozen body, The silk fibroin was gelled, and it was taken out after 2 hours.

[0027] Put the metal mold in an oven, pre-dry it at 30°C for 20 hours, then dry it at 110°C for 5 hours, and make a porous silk fibroin hydroxyapatite composite material after demoulding. Number is 1 #...

Embodiment 2

[0029] Add 4.8g HA to 20ml silk fibroin solution with a concentration of 6%, stir for 10min, then treat it with ultrasonic waves for 10min, add 0.36g silk fiber (pre-degummed, cut into 10mm length), continue stirring for 10min, and form an emulsion in the mixture.

[0030]Pour the mixture into a metal mold, and immediately place the mold in an acetaldehyde bath at -85°C for 5 hours to form a frozen body, and then freeze it at -30°C for 24 hours. The whole mold is immersed in an appropriate amount of acetaldehyde solution, and the acetaldehyde slowly penetrates into the frozen body to make the silk fibroin gel, take it out after 24 hours, and dry it in an oven at 30°C for 20 hours, and then dry it at 120°C for 5 hours , the silk fibroin hydroxyapatite porous composite material was obtained after demoulding, number 2 # , and its properties are shown in Table 1.

Embodiment 3

[0032] The content of HA is 3.6g, the concentration of silk fibroin solution is 9%, the pre-degummed silk fibers are cut into 2mm long, add 0.5g, and use liquid nitrogen to freeze quickly at low temperature for 10min, and other preparation processes are the same as in Example 2 to obtain silk fibroin Apatite porous composite material, number 3 # , and its properties are shown in Table 1.

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PUM

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Abstract

The invention discloses a silk hydroxyapatite composite stock and making method, which is characterized by the following: taking the hydroxyapatite and silk protein as base; taking the degelatinized cultivated silk or cloth as reinforcement; solidifying HA power in more homogeneous solution rapidly by adapting solution blending-freezing gel method; elevating temperature above the glass transition temperature of silk solution; adding low-molecular-weight effumable organic reagent to rearrange the silk protein molecule in the freezing solution; gelifing to fix HA powder; formatting pore of certain structure after detaching the water and organic reagent by heating; forming silk hydroxyapatite composite stock with multipore. The invention qualifies good dynamical property, cellular compatibility and right degradation speed to satisfy the need of cell cultivation bracket as bone injuring renovation, medicine control releasing and bone tissue engineering stock with broad prospect.

Description

technical field [0001] The invention relates to a biomedical material, in particular to a porous composite material with hydroxyapatite and silk fibroin as a matrix and degummed silkworm silk or fabric as a reinforcement and a preparation process thereof. Background technique [0002] Bone defect and nonunion are common clinical sequelae of bone trauma and infection. How to repair bone defect and nonunion has always been a thorny issue in the field of orthopedics. Therefore, bioactive artificial bone materials with excellent properties are often required in surgical operations such as orthopedics, dentistry, cosmetic surgery, and maxillofacial repair. Hydroxyapatite (Hydoxyapatite, referred to as HA), its chemical formula is Ca 10 (PO 4 ) 6 (OH) 2 , is the main inorganic substance that constitutes bones and teeth. Its chemical composition, crystal structure, and physical and chemical properties are similar to those of normal human bone, and it h...

Claims

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Application Information

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IPC IPC(8): A61L27/12A61L27/22A61L27/40
Inventor 卢神州李明忠
Owner SUZHOU UNIV
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