Nanometer hydroxyapatite/natural polymer composite, preparation method and application thereof

A nano-hydroxyapatite and natural polymer technology, applied in the field of preparation of nano-hydroxyapatite, can solve the problems of high inorganic mineral content, unsatisfactory bone tissue engineering scaffold materials, etc., and achieve low cost and simple operation process Viable, Inexpensive Effects

Inactive Publication Date: 2010-04-14
JINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, tissue engineering, especially bone tissue engineering scaffold materials, requires a relatively high content of inorganic minerals, and the tensile and compr...

Method used

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  • Nanometer hydroxyapatite/natural polymer composite, preparation method and application thereof
  • Nanometer hydroxyapatite/natural polymer composite, preparation method and application thereof
  • Nanometer hydroxyapatite/natural polymer composite, preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Mineralization of Chitosan Porous Sponge. The porous chitosan sponge is prepared by freeze-drying 2% (weight) chitosan acetic acid solution at -20° C. for 8 hours. The size of the sponge is 2×2×1 cm, the porosity is about 80%, and the average pore diameter is about 300 μm. Add 5ml of deionized water, 15ml of ethanol and 0.3g of urea into a jar and stir evenly, then add 5ml of sodium dihydrogen phosphate solution (0.1M). Immerse the chitosan sponge in the mixed solution and stir at a low speed for 10 minutes; make the solution fully penetrate into the sponge network, then add 8.35ml calcium chloride solution (0.1M) and continue to stir for 5 minutes, adjust the wide mouth with saturated sodium hydroxide solution The pH value of the reaction system in the bottle was 12. Seal the jar and place it in an oven at 80°C for 24 hours. The chitosan porous sponge after mineralization is fully cleaned with 85% (weight) ethanol aqueous solution, ultrasonic (40KHz) concussion 10min...

Embodiment 2

[0042] Mineralization of phosphorylated chitosan porous scaffolds. Phosphorylated chitosan porous scaffold is made by printing 2% (weight) chitosan acetic acid solution with a three-dimensional printer, and then modifying the phosphorylated surface. It is cylindrical, with a diameter of 1 cm × height of 1 cm, a porosity of 50%, and a pore size of 500 μm; Five phosphorylated chitosan porous scaffolds were placed in a reaction system containing 10ml water, 30ml ethanol and 0.5g urea. Then 10 ml of sodium dihydrogen phosphate solution (0.1 M) and 16.7 ml of calcium chloride solution (0.1 M) were added. After stirring for 5 minutes, the pH value of the system was adjusted to 10 by solid sodium hydroxide. The reaction system was sealed and placed in an oven at 70°C for 24 hours to react. The mineralized scaffold was a nano-hydroxyapatite / phosphorylated chitosan composite material. The mineralized scaffold was fully washed with water, oscillated by ultrasonic (40 KHz) for 10 min, ...

Embodiment 3

[0044] Mineralization of silk protein fiber bundles. The silk protein fiber bundle is prepared by electrospinning, the fiber diameter is less than 1 nm, and the fiber bundle diameter is about 20 μm. Place 0.1g of silk protein fiber bundles in a mixed solution containing 5ml of water, 15ml of ethanol and 0.3g of urea, and vibrate on a shaker at a speed of 60rpm. Then add 8.35ml of calcium chloride solution (0.05M) and 5ml of sodium dihydrogen phosphate solution (0.05M), and adjust the pH value of the system to 12 with saturated sodium hydroxide solution. Seal the reaction system and place it in an oven at 60° C. for 8 hours to obtain a nano-hydroxyapatite / silk protein composite material. The mineralized sponge was fully washed with water, oscillated by ultrasonic (20KHz) for 5min, then frozen at -20°C for 2h, and then freeze-dried. Transmission electron microscopy and scanning electron microscopy of the mineralized material show that the surface of the fiber is tightly covere...

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Abstract

The invention relates to tissue engineering materials and particularly discloses a nanometer hydroxyapatite/natural polymer composite, a preparation method and an application thereof. The preparation method includes the steps of putting the natural polymer composite into the mixed solution of alcohol, water and urea and adding monosodium phosphate solution and calcium chloride solution to conduct sealing reaction and obtain the composite, wherein the volume ratio of the alcohol and the water in the mixed solution is 2-6:1, the concentration of urea in the mixed solution is 1-3g/100ml. The method is quick and effective, the conditions of the reaction system are mild, the reaction method and the reaction system are easy to operate and control, the cost is low and investment of high energy source is saved. The composite obtained by the method has a thicker mineralization layer and therefore higher tensile and compressive strength and also can be applied to the field of the tissue engineering materials requiring high mechanical strength, for example the manufacture of bone tissue engineering materials.

Description

technical field [0001] The invention relates to the preparation of nano-hydroxyapatite, in particular to a nano-hydroxyapatite / natural polymer composite material and its preparation method and application. Background technique [0002] Natural bone tissue is a biocomposite of nano-hydroxyapatite (HAP) and natural polymers, so the composite material of nano-hydroxyapatite has always been a research hotspot, but the exudation of nanoparticles may cause damage to the body. Therefore, biomineralization may be an effective method to improve the biocompatibility of materials while maintaining the mechanical properties of matrix materials. Biomineralization is based on the principle of bionics, using biological macromolecules as templates, and controlling the growth of inorganic crystals through self-assembly or self-organization to replicate the nanocomposite and gradient structures of natural bone tissue. [0003] Natural polymer materials such as chitin, chitosan and its deriva...

Claims

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Application Information

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IPC IPC(8): C08L5/08C08L5/04C08L89/00C08K9/04C08K3/32A61L27/46A61L27/56
CPCC08L89/06A61L2430/02C08L5/08C08L89/00A61L27/46C08L5/04
Inventor 李立华周长忍
Owner JINAN UNIVERSITY
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