56results about How to "Increase alkaline phosphatase activity" patented technology

Methods are disclosed for generating HLA homozygous parthenogenetic human stem cell (hpSC-Hhom) lines from both HLA homozygous and HLA heterozygous donors. These hpSC-Hhom lines demonstrate typical human embryonic stem cell morphology, expressing appropriate stem cell markers and possessing high levels of alkaline phosphatase and telomerase activity. Additionally, injection of these cell lines into immunodeficient animals leads to teratoma formation. Furthermore, in the case of HLA heterozygous donors, the hpSC-Hhom lines inherit the haplotype from only one of the donor's parents. SNP data analysis suggests that hpSC-Hhom lines derived from HLA heterozygous oocyte donors are homozygous throughout the genome as assessed by single-nucleotide polymorphism (SNP) analysis. The protocol as disclosed minimizes the use of animal-derived components, which makes the stem cells more practical for clinical application.

The invention belongs to the field of biomedical materials and in particular relates to a preparation method of a co-crosslinked double network hydrogel scaffold for promoting osteogenic growth. The preparation method comprises the following steps: (1) preparation of GelMA; (2) preparation of photo-crosslinkable OGP; and (3) preparation of the hydrogel scaffold: dissolving 50mg of the GelMA prepared in the step (1) and 20mg of the photo-crosslinkable OGP prepared in the step (2) in 1mL of PBS, uniformly mixing the two, adding 10mg of a light initiator, uniformly mixing the mixture, and irradiating the mixture by ultraviolet rays to prepare the hydrogel scaffold. The hydrogel scaffold prepared by the invention plays obvious roles of promoting increase of bone density and union of bone defects, and can regulate proliferation and differentiation of osteoblast and mineralization of a matrix and improve the alkaline phosphatase activities. The hydrogel scaffold is of a porous structure, so that OGP is co-crosslinked to the surface of the porous scaffold to induce OGP to slowly release, and therefore, actions of the biological scaffold in the fields of orthopedics department such as bone defects and bone graft fusion are achieved.

The invention relates to a preparation method of a dental implant and a composite surface thereof. In the dental implant, medical titanium or titanium alloy is used as a substrate, and porous morphology is prepared on the surface while polydopamine and hydroxyapatite are deposited to obtain a dental implant composite surface with good bioactivity. In the invention, a uniform multi-layer pore oxidation film is quickly formed on the surface of the titanium substrate to obtain nano porous morphology; and by enabling a thin layer of protein analogue (polydopamine) to tightly adhere to the surface, the surface hydrophilicity and the corrosion resistance of the dental implant can be improved. Finally, the hydroxyapatite deposition is induced on the surface to obtain a three-layer composite surface; moreover, the nano porous composite surface can effectively reinforce the adhesion and spreading of osteoblast on the implant surface as well as the alkaline phosphatase activity, early bone integration of the implant is accelerated, and firm combination of the material and the organism interface is realized so as to improve the stability and success rate of the implant.

The present invention relates to an application of iridoid in the preparation of anti-osteoporosis medicines, which belongs to the medicines technical field. The invention provides an application of iridoid which is shown in a general formula I in the preparation of medicines used for treating and/or preventing osteoporosis. Experiments found that ALP activity in UMR106 cells is taken as guidance for tracing and separating an extract of iridoid possessing general formula I, the extract and each separated component are researched from the aspects of osteoblast propagation and antioxidation activity, the iridoid possessing the general formula I has the effects of substantially promoting alkaline phosphatase activity and osteoblast differentiation, so that the iridoid has anti-osteoporosis effect and can be used for preparing the medicines used for treating or preventing osteoporosis. The component of specnuezhenide and a compound G13 possesses the effects of substantially promoting the alkaline phosphatase activity and osteoblast differentiation, and the specnuezhenide has effects for obviously promoting UMR-106 cell proliferation, a better effect is obtained when the specnuezhenide is used for anti-osteoporosis effect.

The invention discloses a cultivation method for improving the quality of grass carps, belonging to the technical field of aquaculture. The quality of the grass carps is remarkably improved by utilizing a mode that grass and compounded feeds are fed together under the condition that the growth period and other cultivation effects of the grass carps are not changed, and the cultivation method is simple in technique, low in investment cost, small in risk, high in operability, easy to master and use and easy to popularize.

The invention provides a Chinese herbal medicinal feed additive for improving the quality of carp flesh, and the Chinese herbal medicinal feed additive is prepared from the following raw material drugs in parts by weight: 2 to 4 parts of eucommia, 2 to 4 parts of dandelion, 3 to 5 parts of angelica root, 3 to 5 parts of yam, and 3 to 5 parts of chrysanthemum. A preparation method of the additive comprises the following steps: weighing the eucommia, the dandelion, the angelica root, the yam and the chrysanthemum Chinese herbal medicinal slices in weight proportion, and respectively crushing into coarse powder; collecting volatile oil from the chrysanthemum coarse powder by using a distillation method; immersing the eucommia, dandelion, angelica root and yam Chinese herbal medicinal coarse powders with water, and decocting for three times with the distilled chrysanthemum dreg with slow fire; combining the three filtrates, and heating to obtain concentrated liquid; and combining the volatile oil and the concentrated liquid, adding antiseptic and water quantitatively, uniformly stirring, filtering, filling and sterilizing to obtain the Chinese herbal medicinal feed additive. The Chinese herbal medicinal feed additive is used for improving the quality of carp flesh, and has the advantages of convenient use, low cost, good effect, safety and non toxic or side effect.

The invention relates to a heterodimer protein of a recombinant human bone morphogenetic protein and an efficient expression and renaturation method of the heterodimer protein. The method comprises the following steps: linking a BMP (Bone Morphogenetic Protein) 7 gene with a BMP2 gene through DNA fragments encoding (GlyGlyGlyGlySer)3 by virtue of a fusion PCR technology, inserting fusion gene into a pHis-NusA plasmid so as to construct a recombinant plasmid, introducing the recombinant plasmid into escherichia coli BL21 (DE3), culturing at 37 DEG C, and inducing the expression by virtue of 0.5mM IPTG. The results show that the BMP7/BMP2heterodimer protein can be efficiently expressed by virtue of the method, the expressed protein is present in a form of an inclusion body and a large number of renaturated proteins can be obtained by virtue of simple purification and renaturation of the inclusion body. According to the method, the operation for obtaining the BMP active protein is simple, the preparation cost is greatly reduced and the method is suitable for large-scale fermentation production and provides the possibility of clinical wide application of BMP in the treatment of bone damage.

The invention relates to a polyether-ether-ketone material and a surface modification method thereof. The method comprises the step of carrying out plasma immersion ion implantation on a polyether-ether-ketone material by taking argon and water vapor as ion sources, wherein the process parameters of the plasma immersion ion implantation technique are as follows: the implantation voltage is 500-1000V, the argon flow is 15-60 sccm, the radio-frequency power is 300-500W, the base pressure is 5*10<-3> Pa, the duty ratio is 30%, the frequency is 30 kHZ, the implantation time is 30-90 minutes, the vacuum chamber temperature is 80-120 DEG C, and after the water vapor is fed, the vacuum chamber pressure is greater than 5*10<-3> Pa and smaller than 1*10<-2> Pa. The surface of the modified polyether-ether-ketone material is of a gully-shaped nanostructure, and the surface of the modified polyether-ether-ketone material is connected with a hydroxyl functional group.

The invention relates to a polyether-ether-ketone material and a surface modification method thereof. The method comprises the steps of performing plasma immersion ion implantation of the surface of the polyether-ether-ketone material with argon as an ion source, and then, soaking the polyether-ether-ketone material treated by plasma immersion ion implantation in a hydrogen peroxide aqueous solution. The surface of the modified polyether-ether-ketone material has a shallow hole-shaped nanometer structure.

The present invention discloses a process for obtaining somatic cell derived embryonic stem cells (encoded by reprogrammed somatic cell nuclei), ES cell-like cells or other types of embryo-derived stem cells by nuclear transplantation, and a process for inducing said stem cells into various differentiated cell types.

The invention relates to a method for expressing a recombinant human bone morphogenetic protein in insect cells. The method comprises the following steps of cloning a mature peptide gene segment of the human bone morphogenetic protein (BMP) to an an improved insect cell-baculovirus protein expression vector; co-transfecting sf9 insect cells; preparing recombinant baculovirus having mature peptide gene segment of the BMP; then transfecting High5 insect cells; highly expressing the BMP mature peptides; and purifying the expressed BMP mature peptides via an Ni affinity column. Detections on in-vitro alkaline phosphatase activity and induced-bone experiments of muscular pouch embedded in mice demonstrate that both expressed BMP2 and BMP7 have activities for inducing ectopic bone formation. By using an improved insect cell-baculovirus protein expression system,, 40 mg purified BMP2 or BMP7 mature peptide can be obtained per litre cultured insect cells; and cost is reduced at least 10 times than that in CHO cells.

The invention relates to the field of microorganisms, and particularly discloses a low temperature efficient corn stalk degradation composite microbial system and an application thereof. The composite microbial system comprises bacteria and fungi. The bacteria comprise a Cellvibrio bacterial genus, an Azospira bacterial genus, an Arcobacter bacterial genus, an Azonexus bacterial genus, a Clostridium bacterial genus and a Paludibacter bacterial genus. The fungi comprise a Lotharella bacterial genus and a Trichosporon bacterial genus. According to the low temperature efficient corn stalk degradation composite microbial system and the application thereof, the bacterial genera with specific components are used as bacterium source samples, culture media adaptive to the system are adopted, the composite microbial system which is composed of the specific bacterial genera is screened and acclimated through enrichment culture, subculture, enzyme production living bacterium culture and corn stalk degradation culture, corn stalks can be efficiently degraded under a low temperature condition through the system, and the defect that an existing microorganism bacterium agent can only degrade the crop stalks under a medium-high temperature condition is overcome.

The invention relates to the field of novel organic fertilizers, and discloses a high-activity small-molecular carbon-rich fertilizer and a preparation method thereof. The high-activity small-molecular carbon-rich fertilizer is prepared from, by weight, 1 part of organic solid waste, 0.6-0.9 part of straw, 0.04-0.1 part of kieselguhr, 0.01-0.1 part of oyster shells and 0.1-0.5 part of wood dust. Compared with the prior art, the high-activity small-molecular carbon-rich fertilizer contains rich amino acids, dipeptide and polypeptide and small-molecular organic matter, does not contain heavy metals, hormones, ova, pathogens and the like and has the advantages of being harmless, small in molecule, high in activity, small in dosage, capable of saving manpower, small in specific gravity, looser and the like. Meanwhile, the high-activity small-molecular carbon-rich fertilizer has the obvious effects of improving soil, promoting plant growth and enhancing plant resistance, inhibiting plant pathogens and the like, and a series of agricultural problems caused by usage of chemical pesticides and chemical fertilizers are thoroughly solved.

The invention relates to a fermentation type compound feed for laying hens. The problems that nutrient resistant components are high, and the discharge quantity of nitrogen elements and ammonia in a poultry house is large, are fundamentally solved. The fermentation type compound feed is characterized by comprising the following components in parts by weight of 564-601 parts of corn, 12.6-31 partsof corn protein powder, 20-45 parts of corn DDGS, 11-28 parts of corn germ meal, 20-35 parts of cottonseed meal, 114-172 parts of soybean meal, 40-80 parts of fermentation raw materials, 74-85 parts of stone powder, 5-7 parts of soybean oil, 6-8 parts of dicalcium phosphate, 2.5-3.0 parts of sodium chloride, 0.4-0.6 part of choline chloride, 1-1.5 parts of methionine, 2.3-5 parts of lysine salt, 0.2-0.3 part of phytase, 10-12 parts of a trace element premix feed, 1-1.1 parts of a vitamin premix feed and 0.2-0.3 part of complex enzymes, wherein the enzyme activity of the phytase is 5000U/g. Thefermentation type compound feed is reasonable to design, the laying rate is increased, the feed-egg ratio is reduced, the discharge quantity of nitrogen elements in excrement and urine is reduced, and the economic benefits of an enterprise are increased.

Provided are compounds of general formula A and A′, wherein X₁ and X₂ are each C, CH or N; R₃ and R₄ are each H, optionally substituted C₁-C₃₀ saturated or unsaturated chemical group, or together form an optionally substituted C₅-C₈ cycle; Z₁; Z₂ and Z₃ are each N or CH; V is C═O, C═S or CH₂; n is from 1 to 12; W₁ and W₂ are each H, CH₂, O or S; and R₁ and R₂ are each H, Cr₁C₆ alkyl, C₁C₆ aryl, C₁C₁₂ alkylaryl, optionally substituted phenyl, C₁C₆ alkoxy, C₁C₆ thioalkoxy, F, Cl, Br or I. These compounds inhibit steroid sulfatase (STS), act as selective estrogen receptor modulators (SERMs), increase alkaline phosphatase (ALP) activity, and are useful in the treatment of medical conditions involving hormones such as breast cancer, prostate cancer, endometriosis, osteoporosis, benign prostatic hyperplasia and endometrial cancer.

The invention belongs to the field of health products and in particular relates to a pharmaceutical composition for increasing bone density and a preparation method thereof. The pharmaceutical composition provided by the invention is prepared from the following raw materials in parts by weight: 2-6 parts of oysters, 0.5-2 parts of malt, 1-4 parts of Chinese yams, 1-3 parts of poria cocos, 1-4 parts of Chinese dates, 0.5-2 parts of liquorice, 0.5-2 parts of endothelium corneum gigeriae, 0.000001-0.000005 part of vitamin D2, 5-15 parts of calcium gluconate, 2-5 parts of sodium L-ascorbic-acid-2-phosphate, 1-4 parts of glutathione, 1-4 parts of phellinus igniarius fermented powder and 2-75 parts of auxiliary materials. The pharmaceutical composition provided by the invention has a good calcium supplementing effect, is rich in nutritional ingredients, safe and effective, convenient to take and applicable to being taken by children for a long time and can promote sustainable growth of the bone density.

The invention discloses selenium-enriched probiotic growing and fattening pig feed keeping metabolic balance and a preparing method thereof. The selenium-enriched probiotic growing and fattening pig feed is characterized by being prepared from, by weight, 500-550 parts of corn kernels, 100-120 parts of coarse cereal flour, 100-120 parts of alfalfa meal, 5-6 parts of alfalfa saponin, 20-23 parts of selenium-enriched probiotics, 15-18 parts of salt, 100-120 parts of vinasse, 30-35 parts of broad bean flour, 10-12 parts of pericarpium citri reticulatae, 20-25 parts of folium ginkgo, 10-12 parts of semen cassiae, 30-35 parts of almonds, 15-18 parts of lophatherum gracile, 5-6 parts of fresh lemon slices, 10-12 parts of lotus leaf powder, 10-12 parts of lilies, 30-35 parts of tomato sauce and 20-23 parts of syrup. The selenium-enriched probiotic growing and fattening pig feed keeping metabolic balance has the advantages of having comprehensive nutrition, savoury, mellow, fine and smooth mouthfeel and a uniform orange color, and the effective ingredients have the effects of keeping metabolic balance, improving immunity, increasing appetite, diminishing inflammation, decreasing internal heat and the like.

The invention provides a fluorine modified polyether-ether-ketone/graphite nano composite material and an artificial joint prosthesis. The fluorine modified polyether-ether-ketone/graphite nano composite material comprises a polyether-ether-ketone/graphite nano composite material and fluorine atoms used for performing surface modification on the polyether-ether-ketone/graphite nano composite material, wherein the fluorine atoms are loaded on the surface of the polyether-ether-ketone/graphite nano composite material through plasma injection. The fluorine modified polyether-ether-ketone/graphitenano composite material has the advantages that the plasma injection technology is further utilized to perform surface fluorine modification on the polyether-ether-ketone/graphite nano composite material to allow fluoride and fluorine-containing functional groups to form on the surface of the polyether-ether-ketone/graphite nano composite material so as to increase the bioactivity of the polyether-ether-ketone/graphite nano composite material, human bones can favorably grow into the surface of polyether-ether-ketone/graphite nano composite material, the combining firmness of a polyether-ether-ketone/graphite nano composite material implant and the human bones, and accordingly the long-term stability of the implant is increased, and the service life of the implant is prolonged.

The invention discloses a selenium-enriched probiotic pig fattening feed for clearing heat, removing toxic materials and killing parasites and a preparation method thereof. The selenium-enriched probiotic pig fattening feed for clearing heat, removing toxic materials and killing parasites is characterized by comprising the following raw materials in parts by weight: 500-550 parts of maize kernels, 100-120 parts of alfalfa meal, 5-6 parts of alfalfa saponins, 20-23 parts of selenium-enriched probiotics, 15-18 parts of salt, 13-15 parts of rice bran oil, 50-55 parts of lotus leaf superfine powder, 60-70 parts of whole wheat flour, 60-65 parts of aloe juice, 150-160 parts of bran, 5-6 parts of honeysuckle, 5-6 parts of freshwater snail shells, 10-12 parts of rice vinegar, 10-12 parts of wild sarcandra glabra, 5-6 parts of flos paulowniae, 5-6 parts of menthol, 5-6 parts of cyrtomium fortunei and 15-18 parts of honey. The effective components of the selenium-enriched probiotic pig fattening feed for clearing heat, removing toxic materials and killing parasites have the functions of clearing the heat, removing the toxic materials, resisting the bacteria, diminishing the inflammation, enhancing the immunity, promoting the digestion, and adjusting the metabolism.

The invention discloses a method of testing amentoflavone (AME) in the preparation of bone tissue engineering seed cells. The method includes: culturing mesenchymal stem cells (MSCs), adding osteogenesis-inducing medium (OIM) or osteogenesis-inducing medium containing different concentrations of amentoflavone, and detecting cell proliferation by an MTT (methyl thiazolyl tetrazolium) method; after the cells completely fuse, adding osteogenesis-inducing medium or osteogenesis-inducing medium containing different concentrations of amentoflavone to carry out culturing, adding a cell lysis solution, detecting activity of alkaline phosphatase with a kit, and measuring protein concentration by a Bio-Rad method; staining the cells with the alkaline phosphatase, and observing and photographing the cells under a microscope; after the cells completely fuse, adding osteogenesis-inducing medium or osteogenesis-inducing medium containing different concentrations of amentoflavone into each hole, and measuring osteocalcin level with an osteocalcin detection kit.

The invention discloses growth-promoting selenium-enriched probiotic pig fattening feed and a preparation method thereof. The growth-promoting selenium-enriched probiotic pig fattening feed is characterized by being prepared from, by weight, 500-550 parts of corn kernels, 100-120 parts of alfalfa meal, 5-6 parts of alfalfa saponins, 20-23 parts of selenium-enriched probiotics, 15-18 parts of table salt, 1-1.2 parts of calcium powder, 10-12 parts of vinegar residues, 30-35 parts of beet pulps, 20-23 parts of water chestnuts, 5-6 parts of cortex mori, 5-6 parts of rhizoma atractylodis, 30-35 parts of mulberry fruit, 8-9 parts of spreading hedyotis herbs, 50-55 parts of fermented oil-tea-cakes, 5-6 parts of rhizoma coptidis, 30-35 parts of cacti, 10-12 parts of butter and 10-12 parts of pectin. The growth-promoting selenium-enriched probiotic pig fattening feed has the advantages of being diverse in nutrition, capable of increasing appetite, helping digestion, regulating metabolism, enhancing immunity, supplementing calcium, improving meat product quality and promoting growth and the like.

The invention discloses selenium-rich probiotics growing and fattening pig feed resisting bacteria, relieving inflammation and facilitating feces excretion and a preparing method of the feed. The feed is characterized by being prepared from, by weight, 500-550 parts of corn kernels, 30-35 parts of chrysanthemums, 20-23 parts of selenium-rich probiotics, 100-120 parts of alfalfa meal, 5-6 parts of alfalfa saponin, 15-18 parts of salt, 30-35 parts of Chinese yam, 50-55 parts of common nepenthes, 100-120 parts of soybean curd residues, 20-23 parts of rice bran protein, 30-35 parts of black rice, 25-30 parts of pumpkin flowers, 10-12 parts of folium eriobotryae, 10-12 parts of onion polysaccharide, 25-30 parts of honeycombs and 10-12 parts of aloe gel. The selenium-rich probiotics growing and fattening pig feed resisting bacteria, relieving inflammation and facilitating feces excretion has the advantages of being diverse in nutrition, rich in taste, delicious in flavor, uniform in particle and the like, and the effective ingredients of the feed have the effects of resisting bacteria, relieving inflammation, facilitating feces excretion, achieving detoxification, enhancing immunity, maintaining balance of gastrointestinal tract microbial florae and the like.