2results about How to "Increase alkaline phosphatase activity" patented technology

This invention belongs to the field of agricultural biotechnology and discloses a low-temperature synergistic biological agent, its preparation method, and its application. The core innovation lies in using a unique engineered Bacillus subtilis strain as the core functional carrier, and performing a ternary targeted compounding with polydeoxyribonucleotides (PDRN) and biostimulants to achieve a triple synergistic low-temperature resistance effect. The engineered Bacillus subtilis strain was deposited at the China General Microbiological Culture Collection Center (CGMCC) on March 11, 2026, with accession number CGMCC No. 37899. The chassis strain of Bacillus subtilis was deposited at the same center on March 3, 2026, with accession number CGMCC No. 37834. The modification strategy involves knocking out the α-subunit gene of acetoin dehydrogenase and the phosphoacetyltransferase gene from the genome of Bacillus subtilis CGMCC No. 37834, and expressing the acetyllactate synthase gene and the α-acetyllactate decarboxylase gene.

The invention relates to the technical field of biological medicines, in particular to a lignan-reducing compound, an extraction method thereof and application of the lignan-reducing compound to a medicine for resisting postmenopausal osteoporosis (PMOP). The invention discloses a lignan-reducing compound, the structural formula of which is # imgabs0. The extraction method of the lignan-reducing compound mainly comprises the following steps: taking dry rhizome of curculigo macrophylla, performing reflux extraction twice with 95% ethanol at 60 DEG C, performing reflux extraction for 3 hours each time, combining extracting solutions, and performing reduced pressure distillation until no extracting solution residue exists, thereby obtaining the lignan-reducing compound. The curculigo macrophylla ethanol extract extractum is obtained. The invention provides a preparation method and an identification process of three novel lignan-reducing compounds for treating PMOP from curculigo macrophylla, which show that the three compounds have good effects of promoting bone formation and regulating bone metabolism.