Low-temperature-resistant synergistic biological agent as well as preparation method and application thereof

By combining genetically engineered Bacillus subtilis with PDRN and biostimulants in a ternary compound, the limitations of existing low-temperature resistance technologies have been overcome, enabling crop growth promotion and soil improvement under low-temperature conditions, thereby enhancing crop low-temperature tolerance and yield.

CN122271331APending Publication Date: 2026-06-26SINOCHEM AGRI LINYI R&D CENT CO LTD +1
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Patent Information

Application Number
CN202610311926.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-13
Publication Date
2026-06-26

AI Technical Summary

Technical Problem

Existing methods for combating low temperatures are costly, have a narrow range of applications, leave chemical residues, and have limited effectiveness with single microbial agents. They are insufficient to address the problems of slow crop cell repair, insufficient energy supply, and rhizosphere microecological imbalance under low temperatures.

Method used

By combining genetically engineered Bacillus subtilis with polydeoxyribonucleic acid (PDRN) and various biostimulants in a ternary complex, a synergistic low-temperature resistance mechanism is formed. This mechanism utilizes the rhizosphere colonization and signaling molecule-mediated effects of Bacillus subtilis to enhance crop stress resistance and soil microecological regulation.

Benefits of technology

It enhances crop low-temperature tolerance, promotes growth, improves soil microecology, increases crop yield, and is environmentally friendly, suitable for a variety of crops and greenhouse cultivation.

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Abstract

The invention belongs to the technical field of agricultural biology, and discloses a low-temperature-resistant synergistic biological preparation as well as a preparation method and application thereof, and the core innovation is that specific bacillus subtilis engineering bacteria are taken as a core functional carrier and are subjected to ternary directional compounding with polydeoxyribonucleotide (PDRN) and biostimulant to realize a triple synergistic low-temperature-resistant effect. The bacillus subtilis engineering bacterium is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 11, 2026, and the preservation number is CGMCC No.37899. The chassis strain bacillus subtilis is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 3, 2026, and the preservation number is CGMCC No.37834. The invention further discloses a preparation method of the bacillus subtilis engineering bacterium. According to the modification strategy, an acetoin dehydrogenase alpha subunit gene and a phosphate acetyl transferase gene are knocked out from a bacillus subtilis CGMCC No.37834 genome, and an acetolactate synthase gene and an alpha-acetolactate decarboxylase gene are expressed.
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