Preparation method of poly (lactic acid-glycolic acid)/hydroxyapatite nanofiber compound bracket for bone repair

A technology of hydroxyapatite and glycolic acid, applied in nanostructure manufacturing, phosphorus compounds, nanotechnology and other directions, can solve the problems of difficult to promote the expression of normal activity of osteoblasts, difficult to obtain cell active sites, etc.

Inactive Publication Date: 2010-04-14
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Traditional bone repair materials are difficult to obtain cell active sites, and it is difficult to promote the expression of normal activity of osteoblasts

Method used

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  • Preparation method of poly (lactic acid-glycolic acid)/hydroxyapatite nanofiber compound bracket for bone repair
  • Preparation method of poly (lactic acid-glycolic acid)/hydroxyapatite nanofiber compound bracket for bone repair
  • Preparation method of poly (lactic acid-glycolic acid)/hydroxyapatite nanofiber compound bracket for bone repair

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0026] 1) Dissolve calcium chloride and diammonium hydrogen phosphate in 50g / L polyethylene glycol aqueous solution respectively, prepare the polyethylene glycol aqueous solution of diammonium hydrogen phosphate with a concentration of 0.1mol / L and the polyethylene glycol solution with a concentration of 0.1mol / L The polyethylene glycol aqueous solution of calcium chloride; the polyethylene glycol aqueous solution of diammonium hydrogen phosphate is added dropwise in the polyethylene glycol aqueous solution of calcium chloride, and the calcium phosphorus volume ratio is controlled to be 1.67, and the pH value is adjusted by magnetic stirring was 10, reacted in a water bath at 0°C for 30 minutes, and aged for 48 hours; ultrasonically washed 3 times with deionized water, frozen at -20°C for 2 hours, and freeze-dried in a lyophilizer for 48 hours to obtain amorphous hydroxyl Apatite particles. The amorphous particles were calcined at 900° C. for 4 hours and ultrasonically oscilla...

example 2

[0033] Step 1) is the same as step 1) in Example 1 to obtain granular hydroxyapatite nanocrystals.

[0034] Step 2) is the same as step 2) in Example 1, but the electrospinning flow rate is set at 0.5ml / h to obtain poly(lactic acid-glycolic acid) nanofibrous membrane support and poly(lactic acid-glycolic acid) / hydroxyapatite nanofibers Composite membrane scaffold.

example 3

[0036] Step 1) is the same as step 1) in Example 1 to obtain granular hydroxyapatite nanocrystals.

[0037] Step 2) is the same as step 2) in Example 1, but the electrospinning voltage is set to be 15kV to obtain poly(lactic acid-glycolic acid) nanofiber membrane support and poly(lactic acid-glycolic acid) / hydroxyapatite nanofiber composite membrane stand.

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Abstract

The invention discloses a preparation method of a poly (lactic acid-glycolic acid)/hydroxyapatite nanofiber compound bracket for bone repair, which comprises the following steps: adding hydroxyapatite nanoparticles into a mixed solvent of tetrahydrofuran and dimethylformamide, and then, adding poly (lactic acid-glycolic acid) to obtain a mixed solution of the hydroxyapatite and the poly (lactic acid-glycolic acid); carrying out electrostatic spinning on the mixed solution; and obtaining the poly (lactic acid-glycolic acid)/hydroxyapatite nanofiber compound bracket for bone repair. The invention has simple and convenient preparation method and wide material sources, simulates the composition and the structure of natural bones of human bodies, introduces the bioactive ceramic material hydroxyapatite into a degradable poly (lactic acid-glycolic acid) substrate to form a compound nanofiber bracket, has the advantages of good biocompatibility, excellent comprehensive performance, convenient use and the like, can effectively promote the adhesion, growth and function expression of bone cells and meet the biological requirements of the bone tissue engineering.

Description

technical field [0001] The invention relates to a preparation method of poly(lactic acid-glycolic acid) / hydroxyapatite nanofiber composite scaffold for bone repair. Background technique [0002] Bone is a general term for a group of biomineral materials, mainly developed in vertebrates. Although the structure and composition of each type of bone vary slightly, they all have a common feature: they are mainly composed of type I collagen fibers, carboxyhydroxyapatite and water. Bone is one of the most complex biomineralization systems and the most typical natural organic-inorganic composite material. The carbab hydroxyapatite crystals in bone are all plate-shaped, with an average length and width of 50nm and 25nm, respectively; the crystal thickness is extremely thin and very consistent, generally 1.5nm to 4.0nm. The main organic phase of bone is collagen fibers, and there are also a small amount of bone sialoprotein, chondroitin sulfate, lipids, peptides, etc. The procollag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/46A61L27/58C01B25/32B82B3/00
Inventor 高长有劳丽红王颖俊朱旸
Owner ZHEJIANG UNIV
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