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Nanometer hydroxyapatite/chitosan/gelatin porous scaffold material and preparation method thereof

A technology of nano-hydroxyapatite and porous scaffold, which is applied in medical science, prosthesis, coating, etc.

Inactive Publication Date: 2006-07-12
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, these composite materials still have their shortcomings. Prepare bone tissue engineering scaffold materials similar to natural bone structure, improve their mechanical properties and biological properties, and improve and control the dispersion and particle size of hydroxyapatite in the scaffold. Size is the focus of research in bone tissue engineering

Method used

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  • Nanometer hydroxyapatite/chitosan/gelatin porous scaffold material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Prepare 50ml of acetic acid solution with a mass percentage concentration of 1%, weigh 1g of chitosan and dissolve it into it with magnetic stirring for 20min to fully dissolve it, and prepare a 2% chitosan solution with a mass concentration.

[0022] (2) Weigh 1 g of gelatin and dissolve it in 25 ml of deionized water at 50° C. to prepare a 4% gelatin solution.

[0023] (3) The two solutions of (1) and (2) were mixed, and magnetically stirred at room temperature for 10 hours.

[0024] (4) Prepare 100ml of 0.25% glutaraldehyde solution, take (3) 7ml of the prepared mixed solution and add 3ml of glutaraldehyde solution to the mixed solution, stir it magnetically for about 50s, and quickly drop it into the 24-well culture plate with a rubber dropper . Stand at 4°C for 12 hours to remove air bubbles in the solution. Then put the culture plate into a low-temperature refrigerator (-60°C) to pre-freeze for 12 hours.

[0025] (5) Place the pre-frozen gel in a freeze dry...

Embodiment 2

[0031] The raw materials used are the same as in the examples.

[0032] Embodiment one (8) is adjusted to take by weighing 2.36gCa (NO 3 ) 2 6H 2 O and 2.28gNa 3 PO 4 12H 2 O was dissolved in 100ml Tris biological buffer respectively (the Ca(NO 3 ) 2 solution and 0.06mol / L Na 3 PO 4 solution); chitosan / gelatin porous scaffold material in Ca(NO 3 ) 2 After soaking in the Tris biological buffer solution for 10 hours, wash it with deionized water for 2-3 times; then soak it in Na 3 PO 4 Tris biological buffer and adjust the pH value of the solution to between 11-13 with NaOH, take it out after 10 hours and rinse it with deionized water for 2-3 times; freeze-dry again.

[0033] The remaining steps are the same as in Embodiment 1.

Embodiment 3

[0035] The raw materials used are the same as the examples

[0036] Adjust the volume ratio of the chitosan / gelatin mixed solution and the glutaraldehyde solution in Implementation 1 (4) to 1:1, that is, take 5ml of the mixed solution and 5ml of the glutaraldehyde solution, mix them with magnetic force for about 40s and quickly use the glue head pipette to drop it into the 24-well plate.

[0037] The remaining steps are the same as in Embodiment 1.

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Abstract

The invention discloses a nano hydroxyapatite / chitose / gelatine composite porous bone tissue engineering cradle material and the preparing method. The material comprises chitose and gelatine with their mass ratio being 3:7-7:3 and the nano hydroxyapatite of 40-80nm deposited on the porous cradle material formed by chitose and gelatine. Preparing the porous cradle material with pore diameter 100-300 ª–m and factor of porosity greater than 90% by using phase separation technique, then immersing it in the Tris buffer solution of Ca(NO3)2 for 10-12 hours, washing with deionized water three times and then immersing it in the Tris buffer solution of Na3PO4 for 10-12 hours, regulating pH value between 11 to 13, washing with deionized water three times and freezing for drying, then getting the nano hydroxyapatite / chitose / gelatine composite porous material by recycling the steps above several times, the size diameter of the hydroxyapatite is about 50nm, the material prepared in this invention possesses good mechanical property and biological compatibility.

Description

technical field [0001] The invention relates to a nano-hydroxyapatite / chitosan / gelatin porous support material and a preparation method thereof, belonging to the bone repair biomaterial technology. Background technique [0002] Bone repair and regeneration in plastic surgery is a common and complex clinical problem. Every year, millions of people around the world suffer from orthopedic diseases due to trauma, and many people die due to the lack of ideal replacement materials. The current bone graft materials mainly include autologous bone, allograft bone, specially treated xenograft bone and artificial synthetic bone materials. However, autologous bone sources are limited and secondary operations are required, and complications after transplantation can reach 8%. Allogeneic bone also has disadvantages such as immunogenicity and pathogenicity. There are infection and immune reactions during allograft transplantation, and may cause other diseases health problems. Finding ide...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/28A61L27/56
Inventor 姚康德李俊杰陈亦平姚晖濮礼臣
Owner TIANJIN UNIV
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