Nanometer hydroxyapatite/chitosan/gelatin porous scaffold material and preparation method thereof

Abstract

The invention discloses a nano hydroxyapatite / chitose / gelatine composite porous bone tissue engineering cradle material and the preparing method. The material comprises chitose and gelatine with their mass ratio being 3:7-7:3 and the nano hydroxyapatite of 40-80nm deposited on the porous cradle material formed by chitose and gelatine. Preparing the porous cradle material with pore diameter 100-300 ª–m and factor of porosity greater than 90% by using phase separation technique, then immersing it in the Tris buffer solution of Ca(NO3)2 for 10-12 hours, washing with deionized water three times and then immersing it in the Tris buffer solution of Na3PO4 for 10-12 hours, regulating pH value between 11 to 13, washing with deionized water three times and freezing for drying, then getting the nano hydroxyapatite / chitose / gelatine composite porous material by recycling the steps above several times, the size diameter of the hydroxyapatite is about 50nm, the material prepared in this invention possesses good mechanical property and biological compatibility.

Description

technical field

[0001] The invention relates to a nano-hydroxyapatite / chitosan / gelatin porous support material and a preparation method thereof, belonging to the bone repair biomaterial technology. Background technique

[0002] Bone repair and regeneration in plastic surgery is a common and complex clinical problem. Every year, millions of people around the world suffer from orthopedic diseases due to trauma, and many people die due to the lack of ideal replacement materials. The current bone graft materials mainly include autologous bone, allograft bone, specially treated xenograft bone and artificial synthetic bone materials. However, autologous bone sources are limited and secondary operations are required, and complications after transplantation can reach 8%. Allogeneic bone also has disadvantages such as immunogenicity and pathogenicity. There are infection and immune reactions during allograft transplantation, and may cause other diseases health problems. Finding ide...

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