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Bone tissue repair ink, composition and bracket, and preparation methods thereof as well as kit

A technology of bone tissue and composition, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of easy loss of cell characteristic protein expression, affecting cell growth, etc., and achieve easy loss, good growth environment, and uniform distribution. Effect

Active Publication Date: 2019-02-26
REGENOVO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the prior art, in the application of bone tissue engineering, the common three-dimensional scaffold has the problem that the expression of some characteristic proteins of cells is easy to lose, which affects the growth of cells on the scaffold

Method used

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  • Bone tissue repair ink, composition and bracket, and preparation methods thereof as well as kit
  • Bone tissue repair ink, composition and bracket, and preparation methods thereof as well as kit
  • Bone tissue repair ink, composition and bracket, and preparation methods thereof as well as kit

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preparation example Construction

[0048] Some embodiments of the present invention provide a method for preparing bone tissue repair ink, the steps of which are as follows:

[0049] (1) A mixture of one or more biodegradable materials is provided.

[0050] (2) A mixture of one or more auxiliary molding materials is provided.

[0051] (3) Select a material formula 1 from one or more biodegradable materials in step (1), select a formula 2 from one or more auxiliary molding materials in step (2), and combine formulas 1 and 2 Slurry to form bone tissue repair ink.

[0052] Further, the biodegradable material in the bone tissue repair ink is hydroxyapatite or tricalcium phosphate, and the mass ratio of hydroxyapatite and tricalcium phosphate in the bone tissue repair ink is about 0:10-10:0 , such as 0:10, 1:9, 2:8, 3:7, 4:6, 5:5, 6:4, 7:3, 8:2, 9:1, 10:0. The mass percentage of the mixture of hydroxyapatite and tricalcium phosphate in the bone tissue repairing ink is 50%-80%. In some preferred embodiments, the ...

Embodiment 1

[0132] This embodiment provides a scaffold for bone tissue repair, which is prepared according to the following steps:

[0133] (1) The mixed powder of β-tricalcium phosphate (β-TCP) and hydroxyapatite (HAP) with a mass ratio of 7:3 is used as a raw material, and a mixture of deionized water and glycerol (a volume ratio of 6: 4) is used as a solvent, sodium polyacrylate is used as a dispersant, and a vacuum defoaming machine is used for gradient centrifugal stirring for 1 h (the maximum speed is set at 3000 rpm) to prepare a slurry with a solid phase content of 38 vol%. Add collagen to the slurry as a bio-adhesive, the addition is 1wt% of the mixed powder, use a vacuum defoamer to mix for 2 hours, and use ultrasonic vibration (frequency 100Hz, time 30min, temperature 30°C), low temperature defoaming ( time 12h, temperature 4°C), to obtain bioactive ceramic slurry, the viscosity of the slurry is lower than 100Pa·S, and the solidification time in air is less than 1min.

[0134]...

Embodiment 2

[0137] This embodiment provides a scaffold for bone tissue repair, which is prepared according to the following steps:

[0138] (1) The mixed powder of β-tricalcium phosphate (β-TCP) and hydroxyapatite (HAP) with a mass ratio of 7:3 is used as a raw material, and a mixture of deionized water and glycerol (a volume ratio of 6: 4) is used as a solvent, sodium polyacrylate is used as a dispersant, and a vacuum defoaming machine is used for gradient centrifugal stirring for 1 h (the maximum speed is set at 3000 rpm) to prepare a slurry with a solid phase content of 38 vol%. Sodium alginate was added to the slurry as a bio-binder in an amount of 0.8wt% of the mixed powder, mixed for 2 hours using a vacuum defoamer, and degassed by ultrasonic vibration (frequency 100Hz, time 30min, temperature 30°C) and low temperature. Bubble (time 12h, temperature 4°C) to obtain bioactive ceramic slurry, the viscosity of the slurry is lower than 100Pa·S, and the solidification time in air is less ...

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Abstract

The invention relates to the field of bone tissue engineering, in particular to bone tissue repair ink, a bone tissue repair composition and a bone tissue repair bracket, and preparation methods thereof as well as a kit. The bone tissue repair ink and a bioactive carrier wrapping cells are used as raw materials, and the bone tissue repair bracket is printed according to a preset three-dimensionalstructure by adopting a biological printer. Meanwhile, living cell printing is realized by adopting the bone tissue repair ink and the bioactive carrier as the raw materials. The cells are uniformly distributed on a bracket model formed by the bone tissue repair ink and difficultly slide down to the bottom of the bracket, so that the problem that the expression of some characteristic proteins of the cells is easily lost in the prior art is effectively solved. The growth of the cells on the bracket is facilitated. A human body bone cell growth environment is better simulated, the cell proliferation, the directional differentiation and the specific protein expression are promoted, the extension and the migration of the cells in the bone tissue bracket and the cell junction establishment arefacilitated, and an organic construction body is formed.

Description

technical field [0001] The invention relates to the field of bone tissue engineering, in particular to a bone tissue repair ink, a composition, a bracket, a preparation method and a kit. Background technique [0002] Currently, bone defects caused by infection, tumor, and trauma are limited by the source of autologous bone and the specificity of bone structure, and it is difficult for traditional bone grafting techniques to prepare personalized scaffold materials according to the structure of the specific injury site, making bone defects It has always been a difficult problem in clinical treatment. This is because traditional methods (including solution casting / ion elution, electrostatic spindle, phase separation / lyophilization, in-situ forming, gas / hole-forming fiber weaving, fiber bonding, melt molding, and air pressure molding) pore method, etc.), lack of stable structure, poor mechanical properties, and residual harmful chemical solvents, it is difficult to realize the ...

Claims

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Application Information

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IPC IPC(8): A61L27/58A61L27/12A61L27/18B33Y70/00B33Y80/00
CPCA61L27/12A61L27/18A61L27/58A61L2430/02B33Y70/00B33Y80/00C08L67/04
Inventor 徐铭恩王玲
Owner REGENOVO BIOTECH
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