53 results about "Cell junction" patented technology

The invention relates to the field of bone tissue engineering, in particular to bone tissue repair ink, a bone tissue repair composition and a bone tissue repair bracket, and preparation methods thereof as well as a kit. The bone tissue repair ink and a bioactive carrier wrapping cells are used as raw materials, and the bone tissue repair bracket is printed according to a preset three-dimensionalstructure by adopting a biological printer. Meanwhile, living cell printing is realized by adopting the bone tissue repair ink and the bioactive carrier as the raw materials. The cells are uniformly distributed on a bracket model formed by the bone tissue repair ink and difficultly slide down to the bottom of the bracket, so that the problem that the expression of some characteristic proteins of the cells is easily lost in the prior art is effectively solved. The growth of the cells on the bracket is facilitated. A human body bone cell growth environment is better simulated, the cell proliferation, the directional differentiation and the specific protein expression are promoted, the extension and the migration of the cells in the bone tissue bracket and the cell junction establishment arefacilitated, and an organic construction body is formed.

Disclosed are methods for making surface plasmon resonance-capable arrays wherein molecules, such as proteins or nucleic acids, or cells, are adhered to a metal substrate. The metal substrates are modified by depositing an omega-modified alkanethiol monolayer to the substrate and then contacting the omega-modified monolayer with a heterobifunctional linking compound. Biomolecules or cells can then be attached to the heterobifunctional linking compound. Also disclosed are arrays wherein glutathione-containing molecules are immobilized on the substrate and GST-containing molecules are then specifically immobilized onto the substrate, taking advantage of the affinity between glutathione and GST.

The present invention is directed to a non-immunogenic cellular composition comprising: a cell having a cell surface and antigenic determinants on the cell surface; an optional linker molecule covalently attached to the cell surface; and a hydrophilic, biocompatible, non-immunogenicity providing compound or polymer (e.g., polyethylene glycol or a derivative thereof) covalently attached to the linker molecule or directly to the cell. In one embodiment, the linker molecule is covalently attached directly to the antigenic determinant on the cell surface. In an alternate embodiment, the linker molecule may be covalently attached to a non-antigenic site on the cell surface, but will camouflage the antigenic determinant on the cell surface. Various uses of the resulting non-immunogenic cell are also provided, including a method of decreasing phagocytosis of a cell, a method of decreasing an adverse reaction to a transfusion, a method of decreasing rejection of a transplanted cell, tissue or organ, and a method of decreasing antibody-induced aggregation of cells.

The invention relates to a preparation method of tissue engineering skin containing appendant organs. In the invention, the preparation method comprises the following steps of: inoculating amniotic mesenchyme stem cells, amniotic mesenchyme stem cells induced to the directions of hair papillae, amniotic epithelial cells and amniotic epithelial cells induced to the directions of the epithelia of the sweat gland into a gel solution, then inoculating the amniotic epithelial cells and the amniotic epithelial cells induced by keratinocyte in a warp direction on the surface of a structure of the hair follicle and the sweat gland after culturing by a dermal layer by induced culture forming the structure of the hair follicle and the sweat gland, and obtaining the tissue engineering skin with the structure of the hair follicle and the sweat gland after culturing. The skin has elasticity, toughness, vigorous cellular metabolism, strong multiplication capacity, sufficient secretion of extracellular matrices, tight linkage of the cuticular layer, the inophragma and the cells of the dermal layer and little possibility of falling off, enhances the success rate of transplantation, can promote the healing of a wound surface, enhances the effects of restoration and replacement and has the function of regulating the immunological rejection of a receptor; by the contained structure of the hair follicle and the sweat gland, the function of the skin is more complete; the adopted amniotic tissues are postpartum wastes and have extensive source, strong cell multiplication capability and multiplepassage frequency; and the invention is beneficial to the mass production of seed cells and lowers the industrialization cost.

A magnetic random access memory (MRAM) device is provided which includes a conductive line configured to induce a magnetic field with a higher magnitude along at least a portion of a magnetic cell junction than along a spacing arranged adjacent to the magnetic cell junction. In some embodiments, the conductive line may include first portions aligned with a plurality of magnetic cell junctions and second portions aligned with spacings arranged between the plurality of magnetic cell junctions. In such an embodiment, the first portions preferably include different peripheral profiles than the second portions. A method for fabricating such an MRAM device is also provided herein. The method may include aligning magnetic cell junctions and first portions of a field-inducing line with each other such that at least part of the first portions of the field-inducing line are configured to conduct a higher density of current than second portions of the field-inducing line.

Vascular scaffolds and methods of fabricating the same are disclosed for tissue engineering of vascular constructs. By combining electrospun matrices with cell sheet technologies, vascular constructs with more mature cell layers can be obtained for reconstruction of blood vessels, heart valves and the like. A engineered smooth muscle cell sheet, wrapped around an electrospun vascular scaffold, is able to provide a mature SMC layer that expresses strong cell-to-cell junction markers and contractile proteins. In addition, preconditioning of the cell sheet covered vascular scaffold maintained cell viability and infiltration into the scaffold.

A device for forming a cellular network is provided. The device comprises a substrate. A plurality of hydrophilic sites are provided on the substrate for occupation by cells. A plurality of hydrophilic tracks are provided on the substrate for guiding formation of cell connections between sites. Also provided are a plurality of hydrophilic regions. The hydrophilic sites and hydrophilic tracks are recessed between the hydrophobic regions. The hydrophilic sites and/or hydrophilic tracks may be provided with a guidance cue for guiding formation of the cellular network. Preferably the guidance cue is a chemical guidance cue, such as an extracellular matrix (ECM) protein. An example of an ECM protein is laminin. The device may be used for forming a cellular network of neurons.

This invention relates to a method of fabricating a semiconductor device. A P well for a cell junction may be formed by performing an ion implantation process employing a zero tilt condition. Stress caused by collision between a dopant and a Si lattice within a semiconductor substrate may be minimized and, therefore stress remaining within the semiconductor substrate may be minimized. Accordingly, Number Of Program (NOP) fail by disturbance caused by stress remaining within a channel junction may be reduced. Further, a broad doping profile may be formed at the interface of trenches by using BF₂ as the dopant when the P well is formed. A fluorine getter layer may be formed on an oxide film of the trench sidewalls and may be used as a boron diffusion barrier. Although a Spin On Dielectric (SOD) insulating layer may be used as an isolation layer, loss of boron (B) may be prevented. Accordingly, an additional ion implantation process for compensating for lost boron (B) may be omitted and a NOP disturbance characteristic may be improved.

The invention discloses a porous chitosan microcarrier as well as a preparation method and an application thereof. The porous chitosan microcarrier takes chitosan as a main component, the diameter ofthe porous chitosan microcarrier is 100-300 mu m, interconnected pores are formed in the surface and the interior of the porous chitosan microcarrier, the diameter of the pores is 10-70 mu m, and theporosity is 95% or higher. The porous chitosan microcarrier is uniform in size, the interconnected pores are formed in the surface and the interior, and the highest porosity can reach 95% or higher. With adoption of the porous chitosan microcarrier for culturing cells, the cells can not only be adhered to the surface of the carrier, but also can enter the internal pores to grow, effective cell-cell connection is formed in three-dimensional space, real three-dimensional culture is realized, in vivo environment is effectively simulated, vitality and function of cells are better maintained, and the microcarrier has good biocompatibility.

The invention discloses a cryopreservation method for protecting cell junction. The method comprises the following steps of: a) culturing Madin Darby canine kidney (MDCK) cells; b) dividing the MDCK cells in the step a) into a normal control group, a cryopreservation group, a trehalose group, an Hsp70 high expression group and an Hsp70+ trehalose group; c) performing filter culture on the normal control group, the cryopreservation group and the trehalose group respectively, performing filter culture and gene transfection on the Hsp70 high expression group, and performing filter culture and gene transfection on the Hsp70+ trehalose group; d) performing cryopreservation on the normal control group, the cryopreservation group, the trehalose group, the Hsp70 high expression group and the Hsp70+ trehalose group; e) growing cells and performing morphologic observation (the cultured cells are observed by a microscope and are subjected to HE staining); f) testing the survival rate of the cells; g) observing and analyzing the cell junction morphology and structure; and h) performing a fluorescein experiment. The method is simple, and convenient to use; and the structure and function of the cell junction after tissues and organs are subjected to cryopreservation can be kept complete, and the damage of the cell junction can be effectively avoided.

Multi-domain peptides including a heparin-binding peptide sequence covalently linked to a linker sequence, which linker sequence is covalently linked to a trifunctional amino acid residue forming a branch with two arms, with substantially similar (homodimeric) cellular attachment peptide sequences covalently linked, directly or through an intermediate, to each branch arm, where the sequences are cell binding analogs of or derived from laminin or a portion of laminin. Further provided are preparations for medical devices, pharmaceutical compositions and methods of using the same.

The invention relates to a measuring method for a silicon solar cell junction depth, which belongs to field of silicon solar cell performance parameter measurement method. The measuring method measures and calculates the silicon solar cell junction depth by employing anode oxidation method and differential weighing calculation method, and includes steps: measuring weight of uneroded phosphorus diffused layer silicon substrate namely cell sheet and square resistance, oxidizing surface of the silicon substrate by the anode oxidation method to obtain a oxidation layer; etching the generated oxidation layer by hydrofluoric acid and measuring the square resistance, repeating the upper step until the square resistance becomes smaller, weighting the silicon substrate. Difference value of the weight and the weight measured at beginning is weight of the diffused layer, average depth of the diffused layer namely the cell junction depth can be obtained through dividing the weight difference value by density of the diffused layer and area of the eroded diffused layer.

The invention discloses application of 4', 5, 7-genistein in preparing rotavirus resisting medicine. The genistein can restrain damage of tyrosine protein kinase to cell junction by restraining activation, caused by rotavirus infection, of tyrosine protein kinase of intestinal epithelial cells, so that the intestinal epithelial cells are prevented from being damaged by rotavirus, the completeness of intestinal mucosa is protected, the symptom of rotavirus diarrhea is obviously reduced, and the lethality is reduced. Thus, when the genistein is used as a main active ingredient, high-efficient and low-toxicity specificity rotavirus diarrhea resisting medicine can be researched and developed hopefully.

The invention discloses a method and device for locking a cell by a fixed wireless terminal. The method includes: obtaining current location information of the fixed wireless terminal when a network service enabling instruction arrives; and judging whether distance between the current location information and reference location information is smaller than or equal to a preset distance threshold, if yes, allowing the fixed wireless terminal to initiate the network service normally, and if no, forbidding the fixed wireless terminal to initiate the network service. The method for locking the cell by the fixed wireless terminal adopts a region of a fixed area, as long as customized preset distance thresholds are the same, even if for different fixed wireless terminal, the ranges of locking cells are certainly the same, and the network effective coverage area determined by the method for locking the cell is only related to the reference location and the distance threshold, thereby preventing the problem that cell locking fails or an optimal cell cannot be used at a cell junction, and ensuring accuracy of the range of cell locking.

A method for patterning a magnetic memory cell junction is provided herein, which includes etching exposed portions of a stack of layers to a level spaced above a tunneling barrier layer of the stack of layers. In addition, the method may include implanting dopants into exposed portions of the stack of layers. For example, the method may include oxidizing and/or nitriding the exposed portions of the stack of layers. In some embodiments, the steps of etching and implanting dopants may form an upper portion of the magnetic cell junction. Alternatively, the method may include alternating the steps of etching and implanting dopants throughout the thickness of the exposed portions of the stack of layers. In either case, the stack of layers may include a magnetic layer which includes a material adapted to prevent the introduction of dopants underlying the tunneling barrier layer during the step of implanting.

The invention discloses a functional food for skin aging resistance and wrinkle resistance and a preparation method thereof. The functional food for skin aging resistance and wrinkle resistance is characterized by comprising the following components in parts by weight: 0.1-10 parts of haematococcus pluvialis, 3-10 parts of vitamin, 16-60 parts of protein peptide and 1-20 parts of a plant powder. The preparation method provided by the invention is simple; the functional food is prepared from haematococcus pluvialis, vitamins, protein peptides and the plant powder. The raw materials are green and natural; no side effect is caused; wherein the components such as haematococcus pluvialis, vitamins, protein peptides and the like have a synergistic effect and have the functions of resisting oxidation and scavenging free radicals, cell connection can be tightened, metabolism speed can be increased, exfoliation of dead skin can be promoted, skin fine wrinkles can be reduced, melanin generationcan be inhibited, and deposition can be prevented;the plant powder possesses health care effect, has a health-care effect on intestines and stomach, can promote digestion of intestines and stomach, expel toxins and beautify faces, and has a positive effect on eliminating wrinkles and fine wrinkles of human bodies.

The invention relates to the technical field of cell observation, and particularly relates to a method for in-situ observation of cell connection between monolayer cells by using a transmission electron microscope. The method comprises the following steps: (1) cell preparation: putting sterilized epoxy resin sheets into a culture plate, centrifuging and resuspending the single-layer cells, uniformly adding the single-layer cells into a substrate with the epoxy resin sheets, and putting the substrate into an incubator for culture; (2) specimen fixing, dehydrating and soaking; (3) area marking,block repairing and pre-embedding; (4) pre-embed block repairing and re-embedding; and (5) slicing, dyeing and electron microscope observation. Through the steps, the original appearance of the monolayer cells can be restored to the greatest extent in situ. Compared with the traditional method, the method has the advantages that the damage to cell connection is reduced; compared with a foreign in-situ embedding method, the resin serving as a carrier for cell growth is simpler and easier to implement compared with a glass slide, and the pre-embedding and re-embedding method is more stable and effective.

The present invention relates to a method for fabricating a semiconductor device with improved refresh time. The method includes the steps of: forming a plurality of gate lines on a substrate; forming a plurality of cell junctions by ion-implanting a first dopant with use of the gate lines as a mask; forming a buffer layer along a gate line profile; and forming a plurality of plug ion-implantation regions in the cell junctions by ion-implanting a second dopant into the substrate under the presence of the buffer layer to thereby from the plugs thereon.

The invention relates to a high-throughput single cell capture and arrangement chip and method and belongs to the field of materials and tissue engineering and particularly relates to high-throughputcapture and arrangement of single cells. The method comprises the following steps: making a substrate surface micro-pattern under the action of protein penetration, performing the high-throughput capture and arrangement of the single cells under the action of membrane pore limitation, and arranging and paving the cells on the complex micro-pattern. The high-throughput single cell capture and arrangement chip and method have the benefits that the capture rate of 85 percent or above of single cells can be realized, and the orderly arrangement and the orderly pavement of the cells on the specified protein pattern are realized; the influence caused by cellular heterogeneity during the large cell population study is avoided, and the method is simple and high in single cell capture rate, has a suitable follow-up culture environment, can be determined to be applied to the study on cell junction, intercellular interaction, cell signal transduction and the like at a single cell level and has avery important effect in the cell engineering and tissue engineering study.

The invention provides a photovoltaic cell junction box comprising a labelling device and a label storage cavity formed in the labelling device. A label storage mechanism is arranged in the label storage cavity. The label storage mechanism can paste stored labels to junction boxes. The label storage cavity further internally comprises a pasting mechanism. The pasting mechanism can paste the labelsto the surfaces of the junction boxes and conduct pressing. A junction box transport cavity is formed below the label storage cavity. A junction box transport mechanism is arranged in the junction box transport cavity. The device is simple in mechanism and easy and convenient to use, and the labels can be automatically pasted to the surfaces of the junction boxes. According to the device, the junction boxes are evenly conveyed out by driving conveyor belts through small-angle rotation of a swinging sector gear; during work, under the condition that no junction box arrives within a long periodof time, none of main working parts of the device operate, and the electric power resource can be saved in a limited manner; and power input of the device is achieved through only one motor, and thedevice cost is reduced.