43 results about "Cell junction" patented technology

A single cell sequencing data interpolation method based on a biclustering and graph convolutional neural network comprises the following steps: 1) respectively downloading matrix-form gene expression data of human and mouse from a gene database, and screening out cells of which the total number of gene expressions is less than 3 and genes of which the total number of cell expressions is less than 200; 2) performing feature selection on the gene expression matrix, finding out cells with consistent growth trend, forming a new two-dimensional matrix, and constructing a cell connection diagram; 3) constructing a BiGCN single-cell interpolation model by using a graph convolutional neural network, learning a nonlinear relationship between cells, dividing a data set into a training set and a test set, training the model by using the training set, and reserving the trained BiGCN single-cell interpolation model; 4) inputting the test set into the trained BiGCN single cell interpolation model to obtain an interpolation result, and evaluating the interpolation result by using an evaluation index; the method has the characteristics that the feature extraction capability is improved, interference of redundant zero expression is avoided, data sparsity is effectively relieved, and the interpolation accuracy is improved.

The invention relates to the field of agricultural bioengineering, in particular to application of an oilseed rape BnaA08G0167600WE gene in enhancing pod shattering resistance of oilseed rape. According to the invention, after a transgenic plant is constructed in oilseed rape by utilizing a modern biotechnological means and the oilseed rape gene BnaA08G0167600WE is knocked out, the silique of the mutant is not easy to crack compared with a wild type under the action of external force, and a separation layer and a wood layer of the cross section of the silique of the mutant have more cell connections compared with the wild type, so that the yield of the mutant is improved. And the generation of the mutants provides an important genetic material for further researching the function of the gene and the effect of the gene in the rape.

Described herein, inter alia, are compositions comprising and methods of making and using endothelial cells (ECs) comprising one or more mitophagy-inducing agents (e.g., rapamycin, SKF-96365, lithium, carbamazepine, latrepirdine, spermidine, resveratrol, sodium valproate, trehalose, kaempferol, metformin, AICAR, nilotinib, ambroxol, curcumin, and VL-004). Also described herein, inter alia, are methods vascular cell therapy, promoting blood vessel formation, and increasing vascular development, angiogenesis, and cell junction via administration of a population of ECs comprising a mitophagy-inducing agent (MIA-ECs).

The invention discloses a method for enhancing mechanical force of CAR-T cells on target cells to overcome mechanical immune checkpoints of SFs and application, and relates to the technical field of crossing of immunotherapy and biomechanics, in particular to CAR-T cells targeting fibroblast activated protein. The invention discloses a CAR-T (chimeric antigen receptor T-T) cell and SFs (small-form-factor s) DNA (deoxyribonucleic acid) nano connector for connecting the CAR-T cell with the SFs, the formation of a CAR cluster and mechanical force transmission are promoted by shortening the membrane spacing of an immune synaptic interface to 16-28nm, the DNA nano connector is formed by self-assembling complementary tetrahedral DNA structures, and the TDS comprises a single-stranded DNA vertex S4 with the length of 24-58 nucleotides. According to the method for enhancing the mechanical force of the CAR-T cells on the target cells to overcome the mechanical immune checkpoints of the SFs and the application, the escape and killing problems of the soft target cells are solved, the soft target cells are efficiently removed, and the killing rate of the CAR-T cells treated by DNJ-17 on the SFs is remarkably increased.

The invention relates to a device (1-1, 1-2) for manometric gas-sorption analysis and for determining the solid density of a material. The device (1-1, 1-2) comprises a temperature-controlled region (2), a dosing system, at least one calibrated dosing volume (3, 3a, 3b), a pressure-measuring system (4, 4a, 4b), which is connected to the calibrated dosing volume (3, 3a, 3b) and has at least one pressure sensor, and a connection (15) for connecting a gas-sorption measuring cell (20a), as well as the gas-sorption measuring cell (20a), which is releasably connected to the connection (15) and is intended for receiving a first sample (21a). The dosing system is formed with a dosing element (9a), an inert-gas connection (10), a measuring-gas connection (11) and a vacuum-pump connection (12). The at least one calibrated dosing volume (3, 3a) is connected to the connection (15) for connecting the gas-sorption measuring cell (20a). The device (1-1, 1-2) also comprises a density-measuring cell (20b) for receiving a second sample (21b). The density-measuring cell (20b) is connected to the at least one calibrated dosing volume (3, 3b). The at least one calibrated dosing volume (3, 3a, 3b) and the density-measuring cell (20b) are arranged within the temperature-controlled region (2). The invention also relates to a combined method of manometric gas-sorption analysis for characterizing a material and determining the solid density of the material using the device (1-1, 1-2).

Molecular signature of a common alopecia state, associated with cell junctions. The present invention relates to a method for the in vitro prognosis and / or diagnosis of a common alopecia state of the scalp in a subject, in particular androgenetic alopecia, comprising at least one step a) of measuring the level of expression, in a biological sample taken from an area suspected of being an alopecia area or of becoming an alopecia area in said subject, of at least one gene selected from CDH1, ACTB, ACTBL2, TUBB, TUBB2A, GSN, MYO3B, MYO5B, MYO6, DSG2, DSG3, DSG4, DSC2, GJB2, GJA1, GJB6, GJA3, TJP2, CLDN8, CLDN10, CLDN19, and optionally of at least one gene selected from CTNNB1 and CTNND2.The invention also relates to an in vitro method for evaluating the effectiveness of a treatment for a common alopecia condition, a cosmetic treatment method, the use of a modulator of said gene(s) mentioned above, as well as a method for identifying a compound enabling the prevention and / or treatment of a common alopecia condition.

A microfluidic chip may comprise a plurality of separate microfluidic regions formed into a chip substrate. Each microfluidic region may be configured to simulate an organ and may comprise at least one inlet and at least one outlet with a microfluidic channel therebetween. Connecting microfluidic pathways may be provided between the separate microfluidic regions. The connecting microfluidic pathways may be configured to be vascularized. The connecting microfluidic pathways may comprise a surface treatment configured to receive endothelial cells. The surface treatment may comprise an extracellular matrix protein coating. Endothelial cells may be disposed within the connecting microfluidic pathways. The endothelial cells may comprise organ-specific endothelial cells that match an organ simulated by at least one of the separate microfluidic regions. The connecting microfluidic pathways may comprise synthetic microvascular networks having non-linear channels with physiologically relevant geometries. The physiologically relevant geometries may comprise bifurcations, varying cross-sectional areas, and convolutions.

The invention relates to a combined depolymerization therapy for enhancing colorectal cancer radiotherapy sensitivity, and belongs to the technical field of biological medicine. Specifically, the invention provides an application of an LDH / EDTA layered double hydroxide material in preparation of an anti-colorectal cancer drug used in combination with radiotherapy. In order to solve the problem that the curative effect of low-level colorectal cancer is low, the invention provides a combined therapy for enhancing the curative effect of radiotherapy through depolymerization treatment by using a zinc-aluminum layered dihydroxide material loaded with ethylenediaminetetraacetic acid. By adopting the method, the connection between cells can be efficiently destroyed, so that the tumor is loosened, the hypoxic environment of the tumor is improved, the sensitivity of the tumor to radiotherapy is finally enhanced, and the curative effect is further enhanced.

Provided for herein are novel signal peptides that direct an encoded protein to cell junctions, recombinant polypeptides comprising the same, nucleic acid molecules encoding the same, pharmaceutical compositions comprising the same, and methods of use thereof.

The invention provides trispecific antibodies having one binding site binding to a target antigen on a cancer cell, pathogen, infected cell or autoreactive cell, and second and third binding sites binding to CD3 and CD28 respectively. Such antibodies can crosslink CD3 and CD28 on the surface of T cells at the cell-to-cell junction with target cells, and trigger CD3-mediated signal transduction (Signal 1) and costimulatory molecule-mediated signal transduction (Signal 2) for activation of T cells resulting in efficient elimination of target cells.

The present disclosure pertains to universal and adoptable cell culture substrates designed for applications in biology, medicine, pharmaceutics, and other relevant fields. The disclosure particularly focuses on processes and methodologies for manufacturing such substrates, which prove advantageous for both non-neural (cells exhibiting robust adhesion and weak cell-cell junctions, as well as cells with regular adhesion and cell-cell junctions) and neural cell sheet engineering. These substrates aim to enhance cell adhesion, stimulate cell growth, and enable rapid and uniform harvesting of cell sheets. Additionally, the present invention introduces a scaffold-free method for tissue engineering, facilitating the creation of a 2D / 3D neural tissue construct featuring unidirectional neuron bundles.

A microfluidic chip may comprise a plurality of separate microfluidic regions formed into a chip substrate. Each microfluidic region may be configured to simulate an organ and may comprise at least one inlet and at least one outlet with a microfluidic channel therebetween. Connecting microfluidic pathways may be provided between the separate microfluidic regions. The connecting microfluidic pathways may be configured to be vascularized. The connecting microfluidic pathways may comprise a surface treatment configured to receive endothelial cells. The surface treatment may comprise an extracellular matrix protein coating. Endothelial cells may be disposed within the connecting microfluidic pathways. The endothelial cells may comprise organ-specific endothelial cells that match an organ simulated by at least one of the separate microfluidic regions. The connecting microfluidic pathways may comprise synthetic microvascular networks having non-linear channels with physiologically relevant geometries. The physiologically relevant geometries may comprise bifurcations, varying cross-sectional areas, and convolutions.

Provided herein are novel signal peptides that direct encoded proteins to cell junctions, recombinant polypeptides comprising the signal peptides, nucleic acid molecules encoding the recombinant polypeptides, pharmaceutical compositions comprising the nucleic acid molecules, and methods of use thereof.

The invention discloses application of a known compound, namely a thiazole carboxamide derivative (with the CAS number of 933886-42-1), in preparation of a medicine for treating acute lung injury / acute respiratory distress syndrome, the compound is an SLC39A1 agonist, zinc ions entering cells can be effectively increased, and by reducing the expression quantity of lung tissue inflammation genes IL-1beta and TNF-alpha of mice, the lung tissue inflammation genes IL-1beta and TNF-alpha can be effectively inhibited, so that the acute lung injury / acute respiratory distress syndrome can be effectively inhibited, and the acute lung injury / acute respiratory distress syndrome can be effectively inhibited. Therefore, lung cell infiltration in ALI / ARDS mice is reduced, oxidative damage and tissue damage of lung tissue, inflammatory factor expression and cell connection are improved, and a new choice is provided for treatment of ALI / ARDS.

Provided for herein are novel signal peptides that direct an encoded protein to cell junctions, recombinant polypeptides comprising the same, nucleic acid molecules encoding the same, pharmaceutical compositions comprising the same, and methods of use thereof.

To create a cell connector for electrically conductively connecting at least one first cell terminal of a first electrochemical cell and at least one second cell terminal of a second electrochemical cell, comprising at least one first contact area, for electrically conductively connecting the cell connector to the at least one first cell terminal, at least one second contact area, for electrically conductively connecting the cell connector to the at least one second cell terminal, and at least one elastically and / or plastically deformable connection area, arranged in the flow path between the at least one first contact area and the at least one second contact area, the cell connector requiring little installation space and allowing a small distance between the electrochemical cells that are electrically conductively connected to one another by the cell connector, it is proposed that the connection area is formed at least partially from a work-hardened metallic material.

The application discloses a kind of high efficiency gentle mammal blastocyst single cell dissociation method.The method includes the following steps: (1) zona pellucida removal: the blastocyst is placed in calcium magnesium table type liquid, and zona pellucida is completely removed by dissolving and physical blowing operation;(2) enzyme digestion incubation: the blastocyst after step (1) is handled and is transferred to 35-37 ℃ pre-equilibrated complex dissociation solution and incubated for 15-30 minutes, during which intermittent blowing is carried out, until the blastocyst is completely dissociated into single cell;(3) washing: the single cell obtained by dissociation is washed using dPBS, and the residual dissociation solution is removed.The method of the application has high efficiency, high activity and simplified operation.The dissociation process is simplified from 3-4 steps to two steps (zona pellucida dissolution+enzyme digestion incubation), the total time is shortened from more than 90 minutes to less than 30 minutes, the dissociation efficiency is significantly improved;Cell viability is increased from about 70% to more than 90%, the cell junction is gently lysed, and cell damage is reduced.

In particular, compositions comprising iPSC-derived parietal cell precursors (iMPCs) generated using NK3 homeobox 1 (NKX3.1; parietal cell fate-determining transcription factor) and methods for their preparation and use are described herein. In addition, in particular, methods for maturing iMPCs into functional parietal cell subtypes, including smooth muscle cells, perivascular cells, and fibroblasts, as well as methods for increasing angiogenesis, angiogenesis, and cell junctions, are described herein. iMPCs mediate the formation of functional blood vessels when transplanted together with endothelial cells (ECs); thus, methods for modeling vascular diseases (e.g., 3D vascular organoids (VOs)) and therapeutic vascularization, comprising a method of administering iMPCs and ECs and a step of administering iMPCs and ECs, are also described herein.

Provided are methods to increase the nephron yield and control over nephron locations in kidney organoids and kidney replacement tissues; the methods achieve spatiotemporal control over the mechanical microenvironment to engineer favorable environments for nephron formation within kidney organoids. This engineered control can be created using microdevices that impose mechanical stress at defined intervals synchronized with cyclical nephron development in vivo, agonists / antagonists of tension-generating biochemical pathways in whole organoids or cells, optogenetic control over tension in stem cell collectives, and / or cell-cell junction mediated transfer of mechanical information between cells, or similar approaches.

Disclosed is a method and system for detecting connection failure of a parallel connection cell, and for a battery that is being discharged by the operation of an external device, by detecting whether there is a section matching the amount of change in the discharge voltage value when a cell connection failure condition occurs due to a pre-prepared current interruption device (CID) operation or an open parallel connection line from the change in the discharge voltage value of the battery being discharged.

The application discloses a kind of peptide functionalized cellulose derivatives targeting A beta and its preparation method and application, belong to the field of biological medicine technology.The peptide functionalized cellulose derivative obtained by connecting A beta targeting peptide with hydroxypropyl cellulose through ester bond can be combined with A beta protein through molecular recognition specificity, and self-assembly is formed into core-shell structure nanomicelle, A beta oligomer is efficiently encapsulated, A beta aggregation and fiberization are inhibited, meanwhile, the interaction between A beta and endothelial cell junction protein is blocked by anti-fouling performance, APEL effect induced by amyloid is inhibited, and the integrity of blood-brain barrier is protected.In addition, the peptide functionalized cellulose derivative has good biocompatibility, can reduce A beta plaque deposition in brain, improve the cognitive function of AD patients, and provides a new type of efficient candidate drug for the treatment of Alzheimer's disease.

The invention belongs to the field of biological medicine, and particularly relates to a bispecific binding protein structure of a T cell connector, nucleic acid, a carrier, a cell and a preparation method and application of the bispecific binding protein structure. The invention provides a bispecific binding protein of a T cell connector, the bispecific binding protein comprises a TCE double antibody structure of an anti-CD3 antibody sequence, a Fab / Fab-Fab Fc structure or a Fab / Fab-scFv Fc structure is used to construct a bispecific antibody of a 2 + 1 valence tumor-associated antigen and a CD3 protein, and the bispecific binding protein has good tumor cell killing activity after being combined with different TAAs.

The invention provides trispecific antibodies having one binding site binding to a target antigen on a cancer cell, pathogen, infected cell or autoreactive cell, and second and third binding sites binding to CD3 and CD28 respectively. Such antibodies can crosslink CD3 and CD28 on the surface of T cells at the cell-to-cell junction with target cells, and trigger CD3-mediated signal transduction (Signal 1) and costimulatory molecule-mediated signal transduction (Signal 2) for activation of T cells resulting in efficient elimination of target cells.

The invention relates to a method for manufacturing a solar cell string, wherein at least two solar cells arranged next to each other are electrically connected. The invention is characterized in that the solar cells are electrically connected by means of a cell connector structure, wherein the cell connector structure is formed with a plurality of linear, parallel cell connection fingers and the cell connection fingers are applied to the two front sides of the adjacent solar cells or the two back sides of the adjacent solar cells by means of a printing process.

The invention belongs to the technical field of organoid, and particularly relates to a core-shell structure vascularized bone organoid and a preparation method and application thereof. The MSCs and the ECs are cultured by adopting an organoid culture mode, the cell spheroid with a'core-shell 'structure is constructed by using the MSCs / ECs with a specific cell proportion, a microenvironment for in-vivo growth of cells can be better simulated, cell-cell connection and a complete extracellular matrix are preserved, the cell spheroid has a structure and a function similar to those of source cells or directionally differentiated stem cells, and the cell spheroid has a good application prospect. And the development process of organs is simulated, so that higher self-renewal and proliferation capabilities are achieved, the metabolic capability of cells is improved, the cell viability is improved, and good proliferation, osteogenesis and vascularization effects are achieved. The vascularized bone organ prepared by the method can be widely applied to preparation of bone repair materials.

Chimeric antigen receptor (CAR) T cells, bispecific adapters that link the CAR-T cells to tumor cells that express carbonic anhydrase IX (CAIX), and methods of treating CAIX-expressing cancers using the same.

Provided herein are novel recombinant polypeptides comprising an endogenous cell junction targeting signal peptide and a payload protein, nucleic acid molecules encoding the recombinant polypeptides, pharmaceutical compositions comprising the nucleic acid molecules, and methods of use thereof.

Housing cover for energy storage module and corresponding energy storage module The present invention relates to a housing cover for a housing (4) for an energy storage module (1), which is intended to receive an arrangement of a plurality of energy storage cells (3), electrically connected to each other by means of cell connectors (2), the energy storage cells (3) forming a degassing surface having degassing openings (301) arranged along the degassing surface, which serve, in the event of an overpressure occurring in a critical situation in the energy storage cell (3) concerned, to allow gases to escape in a controlled manner from the interior of the energy storage cell (3) through the degassing opening (301).According to the invention, the housing cover (402) and the degassing surface form a degassing channel (5) extending towards at least one end of the housing cover (402), preferably towards both ends of the housing cover (402). Figure to be published with the abstract: Fig. 2.

Described herein, inter alia, are compositions comprising and methods of making and using iPSC-derived mural progenitor cells (iMPCs) generated using NK3 Homeobox 1 (NKX3.1; a mural cell fate-determining transcription factor). Also described herein, inter alia, are methods maturing the iMPCs into functional mural cell subtypes, including smooth muscle cells, pericytes, and fibroblasts, as well as methods of increasing vascular development, angiogenesis, and cell junction via the iMPCs. The iMPCs mediate the formation of functional vessels when implanted with endothelial cells (ECs); thus, methods of administering iMPCs and ECs and methods of modeling vascular diseases (e.g., a 3D vascular organoid (VO)) and therapeutic vascularization comprising administering iMPCs and EC are also described herein.