36 results about "Silique" patented technology

Provided in the present invention are a Brassica napus drought-tolerant gene BnaC04ARL and the use thereof. The gene is expressed in a plant to improve drought tolerance of the plant. The gene comprises a nucleotide sequence selected from the group consisting of: a nucleotide sequence shown as SEQ ID NO: 1; and / or a nucleotide sequence encoding an amino acid sequence shown as SEQ ID NO: 2. The overexpression of the gene can improve the viability of Brassica napus under drought conditions, enhance root system development, and improve the thousand-grain weight, the grain yield per plant, the number of siliques per plant, dry weight per plant, silique length, etc.

The invention relates to the technical field of plant genetic engineering, in particular to a transcription factor CsKNAT1 and application thereof in regulation and control of development of tea trees. The nucleotide sequence of the gene is as shown in SEQ ID NO: 1, and the sequence of protein coded by the gene is as shown in SEQ ID NO: 2. Research finds that overexpression of the CsKNAT1 gene can influence the form of plant leaves: the edges of the leaves are promoted to have notches (asymmetric leaves are formed, resulting in increase of sawteeth at the edges of the leaves); the formation of compound leaves can be promoted; leaves on plant stems can grow in a rosette form, and secondary stems are bolted in the middles of rosette leaves; the research also finds that the overexpression of the CsKNAT1 gene can obviously influence the flowering phase and the flower form of the plant; the delay of the flowering phase is promoted; overexpression of the CsKNAT1 gene can further influence development of floral organs and formation of siliques, part of arabidopsis plants have phenotypes that corolla becomes small, stamens are exposed, petals gradually extend and are zigzag in a wavy shape, the length of mature siliques is remarkably shortened by 20%-36.1%, and the phenomenon that the fruits are aborted occurs.

The invention relates to the field of agricultural bioengineering, in particular to application of an oilseed rape BnaA08G0167600WE gene in enhancing pod shattering resistance of oilseed rape. According to the invention, after a transgenic plant is constructed in oilseed rape by utilizing a modern biotechnological means and the oilseed rape gene BnaA08G0167600WE is knocked out, the silique of the mutant is not easy to crack compared with a wild type under the action of external force, and a separation layer and a wood layer of the cross section of the silique of the mutant have more cell connections compared with the wild type, so that the yield of the mutant is improved. And the generation of the mutants provides an important genetic material for further researching the function of the gene and the effect of the gene in the rape.

The invention belongs to the technical field of plant breeding, and particularly relates to a breeding method of high-yield multi-chamber brassica napus. Comprising the following steps: performing hybridization by taking a mustard type rape multi-chamber resource as a female parent and a cabbage type rape as a male parent to obtain an F1 generation, and performing isolated propagation to obtain F2 free seeds; selecting partial brassica napus type single plants from F2 free seeds for selfing propagation to obtain F3-generation seeds, planting the F3-generation seeds according to plant lines, performing selfing propagation, screening out single-plant selfing seeds with multi-chamber pods, continuing selfing, continuously repeating for 2-4 generations, screening out single plants closest to male parent characters, and obtaining high-yield multi-chamber brassica napus resources. The multi-chamber brassica napus resource with the multi-chamber pod rate larger than 60% is obtained through the method, the novel multi-chamber brassica napus resource with the pod length close to that of double No.11 in brassica napus parents is obtained, and the pod length of the novel multi-chamber brassica napus resource reaches 11.01 cm.

The invention discloses an oilseed rape gene BnaKNAT1s and an application thereof in creation of droop-type silique oilseed rape, and belongs to the technical field of plant genetic engineering. The gene comprises two copies of BnaA03.KNAT1 and BnaC03.KNAT1, and the CDS (Coding Sequence) sequences of the gene are respectively shown as SEQ ID NO: 1 and SEQ ID NO: 2. Targeted sgRNA is designed according to homologous regions of first exons of the two genes, nucleotide sequences of the sgRNA are shown as SEQ ID NO: 3 and SEQ ID NO: 4 respectively, CRISPR / Cas9 editing vectors are constructed, agrobacterium tumefaciens is transferred, and then rape is infected, so that a transgenic plant is obtained. And screening a homozygous mutant with two genes subjected to frameshift mutation and without a transgenic element, hybridizing the homozygous mutant with a good variety, and carrying out multi-round backcross and selfing to obtain a new germplasm of the sagittaria silique rape. According to the germplasm, the canopy structure is optimized, the light energy utilization rate and ventilation performance are improved, high-density planting is adapted, and gene resources and core materials are provided for high-yield breeding of oilseed rape.

The application discloses application of a plant fruit shape control gene CmFSI8. The application provides application of a protein CmFSI8 in a-f; a, changing plant fruit shape; b, inhibiting elongation of a plant organ; c, shortening plant leaf length; d, reducing the length-width ratio of a plant leaf; e, reducing plant plant height; and f, reducing fruit length of a plant. A gene CmFSI8 for controlling melon fruit shape is separated. Overexpression of the gene in Arabidopsis thaliana by means of construction of a transformation vector can significantly cause the leaf of the Arabidopsis thaliana to be round and short, the silique to be short, and the plant to be dwarfed, and the gene plays a role in inhibiting elongation of an organ. The trait control gene of the application provides a new gene resource for melon fruit shape control, and is expected to change the fruit shape of melon through conventional means and genetic engineering technology, and has important application value in breeding.

This invention belongs to the field of agricultural biotechnology, specifically relating to a strain of Pseudomonas sp. R10 and its applications. The Pseudomonas sp. R10 provided by this invention is deposited at the China Center for Type Culture Collection (CCTCC), accession number CCTCC M 2024850. This strain exhibits significant control effects against clubroot disease, particularly in rapeseed. Verification through examples shows that Pseudomonas sp. R10 inhibits the germination rate of dormant spores of Pseudomonas by 35.14%. In pot experiments, it reduced the clubroot disease severity index by 53.06%. In field trials, treatment of rapeseed with R10 solution reduced the clubroot disease severity index by 39.45%, and significantly increased plant height, rootstock diameter, fresh weight, number of effective branches, and number of effective siliques in the main inflorescence compared to the control plot. R10 solution treatment also increased rapeseed yield by 59.86% and thousand-grain weight by 7.25%.

The invention belongs to the field of fertilizers, and relates to a functional crop special formula fertilizer, which comprises the following components by weight: 70-130 parts of vinasse, 25-75 parts of corn straw, 10-30 parts of chicken manure, 3-7 parts of a microbial agent EM bacteria, 15-25 parts of an inorganic fertilizer, and 2-8 parts of a functional auxiliary material. The inorganic fertilizer comprises urea, diammonium phosphate, potassium chloride and zinc sulfate; the functional auxiliary materials comprise humic acid, alginate oligosaccharide and chitosan oligosaccharide. The special formula fertilizer disclosed by the invention can obviously increase the single-plant silique number, thousand seed weight and yield of the rape.

The application discloses application of a PLC2 gene in regulating growth and development of castor-oil plant and belongs to the technical field of plant genetic engineering. The application discloses application of the PLC2 gene in regulating plant height, seed quality, pollen vitality, flowering period or silique of the castor-oil plant. It is proved that, compared with wild type plants, the plant height of the PLC2 gene overexpression mutant plants is shorter, the leaf abscission is faster, the main stem ear is longer and the flowering is earlier, the number of pistils and stamens is increased, the pistil stigma is longer, the fruits and seeds are fuller, the glossiness is higher, and the mature seed germination rate is higher. It is shown that the PLC2 gene can be applied to genetic engineering and breeding of the castor-oil plant and has important utilization value in cultivating castor-oil plant varieties with excellent traits.

The invention belongs to the technical field of oilseed rape genetic improvement and genetic engineering, and discloses application of a brassica napus BnaUGE2 gene in regulating and controlling the flowering period of oilseed rape. The BnaUGE2 gene has four homologous copies, and the four homologous copies are respectively as shown in SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 5 and SEQ ID NO. 7. Experiments prove that the BnaUGE2 gene is over-expressed under the background of brassica napus B409, so that a plant can flower in advance, and the pod character is not influenced; on the contrary, the activity of the gene is inhibited through a gene knockout technology, so that flowering delay, pod length increase and grain number reduction of each pod can be caused. On the basis, through expression regulation of the BnaUGE2 gene, fine regulation of the flowering period of the rape can be realized, and new germplasm and gene resources are provided for breeding of a new variety of early-maturing rape.

The invention relates to the field of agricultural bioengineering, in particular to application of an oilseed rape BnaC09T0533300WE gene in changing the pod length of oilseed rape. The rape BnaC09T0533300WE gene is positioned in a C09 chromosome, research finds that the BnaC09T0533300WE gene plays an important role in regulating and controlling the pod length of the rape, so that a transgenic plant is constructed in the rape by utilizing a modern biotechnological means, a gene knockout vector is constructed by designing a knockout target of the rape BnaC09T0533300WE gene, genetic transformation is performed in the rape, and the rape pod length can be regulated and controlled. It is found that the pods can be shortened due to the fact that the function of the BnaC09T0533300WE gene is lost, the BnaC09T0533300WE gene has universality in rape variety cultivation, and a new thought is provided for cultivation of better rape varieties.

This invention belongs to the field of microbial technology, specifically relating to a strain of Microbacterium R1 and its applications. The Microbacterium sp. R1 provided by this invention is deposited at the China Center for Type Culture Collection (CCTCC), with accession number CCTCC M 2024851. This strain exhibits significant control effects against clubroot disease. Verification through examples shows that Microbacterium R1 can inhibit the germination rate of dormant spores of Clubroot by 30.4%. In pot experiments, it can reduce the disease index of clubroot by 48.3%, and in field trials, it can reduce the seedling incidence of clubroot by 30.33% and the disease index by 54.61%. The treated group showed significantly higher rapeseed plant height, growing point height, number of green leaves, total number of leaves, rootstock diameter, fresh weight, effective length of the main inflorescence at the silique stage, and number of effective siliques in the main inflorescence compared to the control group. After treatment with the R1 strain, rapeseed yield increased by 77.53%, and thousand-grain weight increased by 9.4%.

The application discloses a breeding method of a rapeseed radish cytoplasm restorer line independent of a recovery source, and relates to the technical field of crop breeding. The breeding method comprises the following steps: crossbreeding or multi-generation backcrossing of rapeseed and a rapeseed radish cytoplasmic male sterile line to form a radish cytoplasm hybrid F1 generation or a stable new radish cytoplasmic male sterile line; pollinating and inducing the radish cytoplasm hybrid F1 generation or the stable new radish cytoplasmic male sterile line by using a rapeseed double haploid induction line without radish cytoplasm recovery genes; bagging and screening fertile single plants in offspring obtained by pollination; continuously self-crossing the fertile single plants from which the radish cytoplasm recovery genes are induced for 2-3 generations; re-crossing; and if all the offspring after re-crossing is fertile, breeding a stable rapeseed radish cytoplasm restorer line with the radish cytoplasm recovery genes. The application has the advantages of short improvement cycle, high efficiency and low cost, and effectively solves the problems of a long breeding cycle of the radish cytoplasm restorer line, genetic instability of the radish cytoplasm restorer line, and negative effects of short siliques and poor seed setting of the radish cytoplasm restorer line and the radish recovery source.

The invention belongs to the technical field of agricultural biology, and particularly relates to a method for germinating sesbania seeds. The method comprises the following steps: collecting mature sesbania siliques, treating to obtain qualified seeds with uniform particle sizes, carrying out heat treatment on the obtained qualified seeds for 2 hours, grinding the seeds with fine sand to damage seed coats, soaking the seeds in hydrogen peroxide and gibberellin in sequence, and finally putting the soaked seeds in a saturated sodium bicarbonate environment with trace vent holes, germinating in a variable-temperature mode of illumination for 12 hours and darkness for 12 hours. According to the method, seed coat obstacles are broken through multi-step cooperative treatment, germination conditions are optimized, and the germination efficiency of the sesbania seeds can be remarkably improved.

The invention relates to the technical field of molecular biology and genetic breeding, and discloses an SSR molecular marker closely linked with rape main inflorescence pod number traits, the SSR molecular marker is located in a 73.5-79.5 cM interval of a rape genome A06 chromosome, and the corresponding physical position is a 40823976bp-42156034bp area. By amplifying the SSR molecular marker and identifying and analyzing the amplified product, the breeding of the optimal character of the main inflorescence pod number of the early rape can be realized. When the SSR molecular marker and the primer sequence provided by the invention are used for breeding the rape, the breeding period is shortened, the production cost is saved, the breeding accuracy is improved, the breeding efficiency is greatly improved, and a new method is provided for breeding the good variety of rape.

The invention relates to the technical field of plant sexual reproduction, in particular to a plant embryo in-vitro rescue method which comprises the following steps: selecting a plant female parent needing embryo rescue; preparing a treating fluid containing a plant hormone regulator; fixing a continuous release device on a branch below the silique or legume of the maternal plant; the treatment liquid is applied to the continuous release device, so that active ingredients in the treatment liquid are absorbed by the branches and transported to the siliques or pods. The externally applied plant hormone regulator can be upwards transported to ovules through the phloem and the xylem and directly acts on the hybrid embryo with development stagnation. Hormonal balance in the embryo is remodeled through cooperative regulation and control of hormones, and a stagnation signal is reversed into a development signal, so that the normal development process of the embryo is rescued and completed in situ without in-vitro operation, and the method is simple to operate, high in success rate, time-saving and labor-saving, and convenient to popularize and apply.

The application discloses a Pinus massoniana tandem zinc finger structure PmTZF1 gene and an expression protein and application thereof, and relates to the technical field of plant genetic engineering.The Pinus massoniana tandem zinc finger structure PmTZF1 gene is disclosed for the first time, a nucleotide sequence of which is shown as SEQ ID NO.1, and an amino acid sequence of which is shown as SEQ ID NO.2.The application constructs an expression vector pBI121-PmTZF1 of the Pinus massoniana tandem zinc finger structure PmTZF1 gene; the constructed expression vector of the Pinus massoniana tandem zinc finger structure PmTZF1 gene is transformed into Arabidopsis; and a transgenic Arabidopsis plant with improved non-biological stress resistance is obtained through cultivation and screening.Compared with a wild type plant, the root of the transgenic strain is significantly longer than that of the wild type, and the transgenic strain blooms and sprouts earlier; under drought stress, the transgenic strain normally blooms and bears fruits, has more branches, and has more silique quantity, length and other properties, which are significantly better than those of the wild type strain.

The invention discloses application of an arabidopsis thaliana gene AT1G12064 in arabidopsis thaliana breeding and a method, and belongs to the field of gene engineering. Through verification, the gene negatively regulates and controls the size and weight of arabidopsis seeds and the number of grains per pod, and positively regulates and controls the pod length, the flowering period and the growth period. A mutant of the gene is knocked out, the seed length, width, area, grain weight, grain number per pod and single plant yield are remarkably increased, the flowering period and the growth period are shortened, and early maturing and high yield are achieved. New gene resources and technical support are provided for early-maturing and high-yield breeding of cruciferous crops such as arabidopsis thaliana, oilseed rape and Chinese cabbage, and the application prospect is wide.

The invention discloses an ecological prevention and control method for sclerotinia rot of colza based on microbial metabolites, and relates to the technical field of agricultural biology. The method comprises the following steps: constructing a microbial team consisting of bacillus subtilis X-01, streptomyces gall YJ1 and coniothyrium minitans parasitic bacteria, wherein the microbial team respectively secretes iturinA and sclerotia to form inhibition antibiotics and parasitic enzymes; extracting and compounding the metabolites to form a synergistic network; preparing a composite carrier composed of an inner core loading layer mesoporous silica, a middle pH sensitive hydrogel layer and an outer hydrophobic ethyl cellulose layer; and accurately applying a quick-release type suspending agent in an initial flowering stage, a pH-responsive type suspending agent in a full-bloom stage and a long-acting slow-release type suspending agent in a pod stage in stages in the whole growth period. A metabolite collaborative network is delivered through a carrier system, and a staged strategy is matched, so that the problems of single strain, poor metabolite stability and short prevention and control period in the prior art are solved, and efficient, stable and ecological prevention and control of sclerotinia rot of colza in the whole growth period are realized.

The application belongs to the technical field of genetic engineering, and particularly relates to application of BnCDS1 gene in plant disease prevention and control and / or growth and an sgRNA combination. The application edits the BnCDS1 gene of rapeseed, and the mutant rapeseed is not different from the wild type Westar in aspects of seedling stage plant phenotype, chlorophyll content, light energy conversion efficiency, nitrogen content and flowering time, but the plant height becomes shorter with the increase of the copy editing number, and the silique becomes smaller and shorter. It is indicated that BnCDS1 is involved in the regulation of rapeseed growth, seed setting and other traits. The edited BnCDS1 rapeseed mutant is inoculated with Sclerotinia sclerotiorum, Botrytis cinerea and Plasmodiophora brassicae, and it is found that the edited rapeseed has enhanced resistance to Sclerotinia sclerotiorum, Botrytis cinerea and Plasmodiophora brassicae, and the higher the editing copy number is, the stronger the resistance is. The BnCDS1 gene edited mutant shows a certain broad-spectrum disease resistance, and the BnCDS1 gene can be used as a new gene resource for green prevention and control of rapeseed diseases.

This invention discloses a silique-specific promoter for plants. The SSEp promoter disclosed in this invention comprises: at least nucleotides 1801-3300 of Sequence 1 in the sequence listing at its 3′ end; and extending from position 1802 of Sequence 1 towards its 5′ end, following the nucleotide sequence of Sequence 1, to obtain any DNA fragment of length from 1500 to 3300 bp; this DNA molecule possesses promoter function. Experiments have demonstrated that the SSEp of this invention possesses promoter function and can specifically drive the expression of target genes in plant siliques, especially in the septum and carpel. The promoter of this invention has excellent application prospects.

The application belongs to the field of oilseed rape breeding, and particularly relates to application of an oilseed rape silique length and grain weight related gene BnaC01.CCT8 in oilseed rape breeding BnaC01.CCT8 . The application clones a gene for simultaneously controlling oilseed rape silique length and grain weight, the gene encodes an amino acid sequence as shown in SEQ ID NO. 2. Overexpression of the gene in Arabidopsis and oilseed rape through genetic engineering technology can significantly improve the silique length and grain weight of Arabidopsis and oilseed rape BnaC01.CCT8 . Hap1 is identified as an excellent haplotype of the gene through haplotype analysis BnaC01.CCT8 . Genetic regulation aiming at the gene can be applied to improvement of oilseed rape yield breeding, and provides a brand new gene resource for basic research and breeding utilization of oilseed rape silique length and grain weight.

This invention discloses a lung-nourishing Solomon's Seal Ointment, its preparation method, and its application, relating to the field of traditional Chinese medicine technology. The herbal composition comprises three herbs: Herb A, Herb B, and Herb C. Herb A includes Solomon's Seal, Lycium barbarum, Chrysanthemum indicum, Mulberry fruit, White peony root, Rehmannia glutinosa, Raw Chinese yam, Ziziphus jujuba peel, Moutan bark, and Poria cocos. Herb B includes Achyranthes bidentata, Polygonatum sibiricum (processed with wine), Ophiopogon japonicus, Adenophora stricta, Tangerine peel, Prepared licorice root, Astragalus membranaceus, and Anemarrhena asphodeloides. The lung-nourishing Solomon's Seal Ointment is prepared by combining Herbs A and B in the herbal composition, decocting them in water, filtering the filtrate, concentrating the liquid, adding Herb C to dissolve, and then simmering over low heat to form an ointment. This technical solution is applied to the treatment of chronic obstructive pulmonary disease (COPD), forming a multi-target, holistic conditioning system. It provides crucial synergistic value to Western medicine's strategy of simply controlling symptoms in improving chronic COPD symptoms and enhancing quality of life, thereby improving the quality of life for COPD patients.

The invention discloses application of AP4B protein and related biological materials thereof in regulation and control of plant drought tolerance. Research on drought-enduring phenotypes of arabidopsis thaliana AP4B homozygous mutants ap4b-3 and ap4b-4 and wild type arabidopsis thaliana finds that AP4B gene mutation can cause drought-sensitive phenotypes, which are specifically shown as follows: compared with the wild type arabidopsis thaliana, the drought-enduring phenotypes of the arabidopsis thaliana AP4B homozygous mutants ap4b-3 and ap4b-4 under a drought treatment condition can cause drought-enduring phenotypes of the wild type arabidopsis thaliana the survival rate of the ap4b mutant, the expression level of the stress response marker gene and the light energy utilization efficiency are obviously reduced. In addition, the invention also finds that AP4B gene mutation can cause plant height reduction, pod length reduction and leaf reduction. The AP4B protein has the functions of regulating and controlling plant drought tolerance, plant height, leaf size and pod length. Gene resources are provided for cultivation of new drought-resistant varieties of crops, a foundation is laid for further research on functions of the AP4B gene in other crops, and the application value is important.

The application provides application of calmodulin in regulation of seed quantity in Arabidopsis thaliana siliques, and the seed quantity in the siliques of Arabidopsis thaliana is increased by applying calmodulin to the culture environment outside Arabidopsis thaliana cells.

The invention provides a rape silique black spot control agent and a preparation method thereof, and belongs to the technical field of vegetable planting. The pesticide for preventing and treating the black spot of the rape pods comprises the following raw materials in parts by weight: 10-20 parts of a natural plant extract, 0.3-0.5 part of a nano-carrier, 4-6 parts of a bacillus subtilis suspension, 5-9 parts of a dispersing agent and 2-3 parts of a stabilizer, the natural plant extract comprises tea tree oil, aloe extract and peppermint oil; the nano-carrier is a star-shaped cationic polymer with pentaerythritol as a core and poly (dimethylaminoethyl methacrylate) as a branched chain. By means of the direct antibacterial effect of the natural plant extract, the biological control mechanism of the bacillus subtilis and the slow release and targeted delivery functions of the nano-carrier, efficient control over the brassica juncea silique is achieved, and a new thought and method are provided for green control over the brassica juncea silique.

The invention belongs to the technical field of gene engineering, and particularly relates to application of an overexpressed OsTTG1 gene in regulating and controlling the seed size and pod length of arabidopsis thaliana, the nucleotide sequence of the OsTTG1 gene is as shown in SEQ ID NO: 1, and the coding amino acid sequence is as shown in SEQ ID NO: 2. According to the present invention, the OsTTG1 gene is subjected to overexpression in the arabidopsis thaliana ttg1 mutant so as to construct the transgenic line OsTTG1-OE; experiments show that compared with the ttg1 mutant, the seeds of the transgenic line are increased, and the silique length is increased. RNA-seq analysis shows that the OsTTG1 promotes the development of seeds and siliques by up-regulating a brassinolide synthesis pathway gene CYP72C1 and regulating and controlling seed development related genes such as TTG2, ABI5 and the like. The invention provides a new target for high-yield breeding of crops, and can be used for cultivating arabidopsis varieties with large seeds and long siliques.

This invention discloses the application of the BnaA01.SnRK1.1 gene in Brassica napus. The BnaA01.SnRK1.1 gene is expressed in various tissues, but its expression level is highest in roots, leaves, flowers, and siliques. Its encoded protein is located in the cell membrane and nucleus. Studies have found that overexpression of the BnaA01.SnRK1.1 protein kinase-encoding gene in plants results in shorter and wider siliques, an increase in the number of siliques per plant, and an increase in yield per plant. Therefore, the BnaA01.SnRK1.1 protein kinase can be used to regulate plant architecture and yield. Overexpression of the BnaA01.SnRK1.1 protein in plants can lead to high-yielding rapeseed, which is of great significance for improving crop traits and increasing yield.

The invention discloses a molecular marker closely linked with a rape pod length character and application of the molecular marker, and relates to the field of plant molecular biology. The molecular marker provided by the invention is C4-ID28, and comprises a forward primer and a reverse primer of which the nucleotide sequences are respectively shown as SEQ ID NO.1 and SEQ ID NO.2. Through identification, the primer of the molecular marker can be used for genotype identification in the early stage of breeding, and when the amplification result of the primer is a single electrophoretic band, the single plant is judged to be in a short-silique phenotype; and if the amplification result is two electrophoretic bands, determining that the single plant is in a long-silique phenotype. The primer can accurately screen the silique length phenotype, significantly improve the selection efficiency and reduce the field workload, thereby accelerating the breeding process.