The present invention belongs to the technical field of
rapeseed planting, and discloses a method for identifying and evaluating the
cold tolerance of
Brassica napus during the flowering period, which comprises the following steps: First, when
Brassica napus is in the flowering period, it is subjected to sub-zero low-
temperature stress treatment, and after the treatment is completed, the plants are moved back to the
greenhouse to resume growth; Then, 5 days after the plants resume growth, the
cold damage phenotypic symptoms of the upper stem leaves, flower buds, petals, main
inflorescence, branched
inflorescence, fruit stalks, young siliques, and long siliques of the plants are collected, and the corresponding phenotypic symptoms are scored; Finally, the low-temperature tolerance level of the
plant is determined according to the total
score. Starting from the changes in the phenotypic symptoms of
Brassica napus plants under low-
temperature stress, the present invention visually observes the phenotypic traits of Brassica napus, and constructs an
evaluation system for Brassica napus under low-
temperature stress during the flowering period; The operating conditions are easy to meet, easy to operate, convenient and fast. Through field production tests, the results are accurate and reliable, and can provide
germplasm reference for the breeding of
rapeseed varieties with
cold tolerance during the flowering period.