17 results about "Silique" patented technology

Provided in the present invention are a Brassica napus drought-tolerant gene BnaC04ARL and the use thereof. The gene is expressed in a plant to improve drought tolerance of the plant. The gene comprises a nucleotide sequence selected from the group consisting of: a nucleotide sequence shown as SEQ ID NO: 1; and / or a nucleotide sequence encoding an amino acid sequence shown as SEQ ID NO: 2. The overexpression of the gene can improve the viability of Brassica napus under drought conditions, enhance root system development, and improve the thousand-grain weight, the grain yield per plant, the number of siliques per plant, dry weight per plant, silique length, etc.

The invention relates to the field of agricultural bioengineering, in particular to application of an oilseed rape BnaA08G0167600WE gene in enhancing pod shattering resistance of oilseed rape. According to the invention, after a transgenic plant is constructed in oilseed rape by utilizing a modern biotechnological means and the oilseed rape gene BnaA08G0167600WE is knocked out, the silique of the mutant is not easy to crack compared with a wild type under the action of external force, and a separation layer and a wood layer of the cross section of the silique of the mutant have more cell connections compared with the wild type, so that the yield of the mutant is improved. And the generation of the mutants provides an important genetic material for further researching the function of the gene and the effect of the gene in the rape.

The invention discloses an oilseed rape gene BnaKNAT1s and an application thereof in creation of droop-type silique oilseed rape, and belongs to the technical field of plant genetic engineering. The gene comprises two copies of BnaA03.KNAT1 and BnaC03.KNAT1, and the CDS (Coding Sequence) sequences of the gene are respectively shown as SEQ ID NO: 1 and SEQ ID NO: 2. Targeted sgRNA is designed according to homologous regions of first exons of the two genes, nucleotide sequences of the sgRNA are shown as SEQ ID NO: 3 and SEQ ID NO: 4 respectively, CRISPR / Cas9 editing vectors are constructed, agrobacterium tumefaciens is transferred, and then rape is infected, so that a transgenic plant is obtained. And screening a homozygous mutant with two genes subjected to frameshift mutation and without a transgenic element, hybridizing the homozygous mutant with a good variety, and carrying out multi-round backcross and selfing to obtain a new germplasm of the sagittaria silique rape. According to the germplasm, the canopy structure is optimized, the light energy utilization rate and ventilation performance are improved, high-density planting is adapted, and gene resources and core materials are provided for high-yield breeding of oilseed rape.

The invention relates to the field of agricultural bioengineering, in particular to application of an oilseed rape BnaC09T0533300WE gene in changing the pod length of oilseed rape. The rape BnaC09T0533300WE gene is positioned in a C09 chromosome, research finds that the BnaC09T0533300WE gene plays an important role in regulating and controlling the pod length of the rape, so that a transgenic plant is constructed in the rape by utilizing a modern biotechnological means, a gene knockout vector is constructed by designing a knockout target of the rape BnaC09T0533300WE gene, genetic transformation is performed in the rape, and the rape pod length can be regulated and controlled. It is found that the pods can be shortened due to the fact that the function of the BnaC09T0533300WE gene is lost, the BnaC09T0533300WE gene has universality in rape variety cultivation, and a new thought is provided for cultivation of better rape varieties.

The application discloses a breeding method of a rapeseed radish cytoplasm restorer line independent of a recovery source, and relates to the technical field of crop breeding. The breeding method comprises the following steps: crossbreeding or multi-generation backcrossing of rapeseed and a rapeseed radish cytoplasmic male sterile line to form a radish cytoplasm hybrid F1 generation or a stable new radish cytoplasmic male sterile line; pollinating and inducing the radish cytoplasm hybrid F1 generation or the stable new radish cytoplasmic male sterile line by using a rapeseed double haploid induction line without radish cytoplasm recovery genes; bagging and screening fertile single plants in offspring obtained by pollination; continuously self-crossing the fertile single plants from which the radish cytoplasm recovery genes are induced for 2-3 generations; re-crossing; and if all the offspring after re-crossing is fertile, breeding a stable rapeseed radish cytoplasm restorer line with the radish cytoplasm recovery genes. The application has the advantages of short improvement cycle, high efficiency and low cost, and effectively solves the problems of a long breeding cycle of the radish cytoplasm restorer line, genetic instability of the radish cytoplasm restorer line, and negative effects of short siliques and poor seed setting of the radish cytoplasm restorer line and the radish recovery source.

The invention belongs to the technical field of agricultural biology, and particularly relates to a method for germinating sesbania seeds. The method comprises the following steps: collecting mature sesbania siliques, treating to obtain qualified seeds with uniform particle sizes, carrying out heat treatment on the obtained qualified seeds for 2 hours, grinding the seeds with fine sand to damage seed coats, soaking the seeds in hydrogen peroxide and gibberellin in sequence, and finally putting the soaked seeds in a saturated sodium bicarbonate environment with trace vent holes, germinating in a variable-temperature mode of illumination for 12 hours and darkness for 12 hours. According to the method, seed coat obstacles are broken through multi-step cooperative treatment, germination conditions are optimized, and the germination efficiency of the sesbania seeds can be remarkably improved.

The application discloses a Pinus massoniana tandem zinc finger structure PmTZF1 gene and an expression protein and application thereof, and relates to the technical field of plant genetic engineering.The Pinus massoniana tandem zinc finger structure PmTZF1 gene is disclosed for the first time, a nucleotide sequence of which is shown as SEQ ID NO.1, and an amino acid sequence of which is shown as SEQ ID NO.2.The application constructs an expression vector pBI121-PmTZF1 of the Pinus massoniana tandem zinc finger structure PmTZF1 gene; the constructed expression vector of the Pinus massoniana tandem zinc finger structure PmTZF1 gene is transformed into Arabidopsis; and a transgenic Arabidopsis plant with improved non-biological stress resistance is obtained through cultivation and screening.Compared with a wild type plant, the root of the transgenic strain is significantly longer than that of the wild type, and the transgenic strain blooms and sprouts earlier; under drought stress, the transgenic strain normally blooms and bears fruits, has more branches, and has more silique quantity, length and other properties, which are significantly better than those of the wild type strain.

The invention discloses application of an arabidopsis thaliana gene AT1G12064 in arabidopsis thaliana breeding and a method, and belongs to the field of gene engineering. Through verification, the gene negatively regulates and controls the size and weight of arabidopsis seeds and the number of grains per pod, and positively regulates and controls the pod length, the flowering period and the growth period. A mutant of the gene is knocked out, the seed length, width, area, grain weight, grain number per pod and single plant yield are remarkably increased, the flowering period and the growth period are shortened, and early maturing and high yield are achieved. New gene resources and technical support are provided for early-maturing and high-yield breeding of cruciferous crops such as arabidopsis thaliana, oilseed rape and Chinese cabbage, and the application prospect is wide.

The invention discloses an ecological prevention and control method for sclerotinia rot of colza based on microbial metabolites, and relates to the technical field of agricultural biology. The method comprises the following steps: constructing a microbial team consisting of bacillus subtilis X-01, streptomyces gall YJ1 and coniothyrium minitans parasitic bacteria, wherein the microbial team respectively secretes iturinA and sclerotia to form inhibition antibiotics and parasitic enzymes; extracting and compounding the metabolites to form a synergistic network; preparing a composite carrier composed of an inner core loading layer mesoporous silica, a middle pH sensitive hydrogel layer and an outer hydrophobic ethyl cellulose layer; and accurately applying a quick-release type suspending agent in an initial flowering stage, a pH-responsive type suspending agent in a full-bloom stage and a long-acting slow-release type suspending agent in a pod stage in stages in the whole growth period. A metabolite collaborative network is delivered through a carrier system, and a staged strategy is matched, so that the problems of single strain, poor metabolite stability and short prevention and control period in the prior art are solved, and efficient, stable and ecological prevention and control of sclerotinia rot of colza in the whole growth period are realized.

This invention discloses a lung-nourishing Solomon's Seal Ointment, its preparation method, and its application, relating to the field of traditional Chinese medicine technology. The herbal composition comprises three herbs: Herb A, Herb B, and Herb C. Herb A includes Solomon's Seal, Lycium barbarum, Chrysanthemum indicum, Mulberry fruit, White peony root, Rehmannia glutinosa, Raw Chinese yam, Ziziphus jujuba peel, Moutan bark, and Poria cocos. Herb B includes Achyranthes bidentata, Polygonatum sibiricum (processed with wine), Ophiopogon japonicus, Adenophora stricta, Tangerine peel, Prepared licorice root, Astragalus membranaceus, and Anemarrhena asphodeloides. The lung-nourishing Solomon's Seal Ointment is prepared by combining Herbs A and B in the herbal composition, decocting them in water, filtering the filtrate, concentrating the liquid, adding Herb C to dissolve, and then simmering over low heat to form an ointment. This technical solution is applied to the treatment of chronic obstructive pulmonary disease (COPD), forming a multi-target, holistic conditioning system. It provides crucial synergistic value to Western medicine's strategy of simply controlling symptoms in improving chronic COPD symptoms and enhancing quality of life, thereby improving the quality of life for COPD patients.

The application provides application of calmodulin in regulation of seed quantity in Arabidopsis thaliana siliques, and the seed quantity in the siliques of Arabidopsis thaliana is increased by applying calmodulin to the culture environment outside Arabidopsis thaliana cells.

This invention discloses the application of the BnaA01.SnRK1.1 gene in Brassica napus. The BnaA01.SnRK1.1 gene is expressed in various tissues, but its expression level is highest in roots, leaves, flowers, and siliques. Its encoded protein is located in the cell membrane and nucleus. Studies have found that overexpression of the BnaA01.SnRK1.1 protein kinase-encoding gene in plants results in shorter and wider siliques, an increase in the number of siliques per plant, and an increase in yield per plant. Therefore, the BnaA01.SnRK1.1 protein kinase can be used to regulate plant architecture and yield. Overexpression of the BnaA01.SnRK1.1 protein in plants can lead to high-yielding rapeseed, which is of great significance for improving crop traits and increasing yield.

The invention discloses a molecular marker closely linked with a rape pod length character and application of the molecular marker, and relates to the field of plant molecular biology. The molecular marker provided by the invention is C4-ID28, and comprises a forward primer and a reverse primer of which the nucleotide sequences are respectively shown as SEQ ID NO.1 and SEQ ID NO.2. Through identification, the primer of the molecular marker can be used for genotype identification in the early stage of breeding, and when the amplification result of the primer is a single electrophoretic band, the single plant is judged to be in a short-silique phenotype; and if the amplification result is two electrophoretic bands, determining that the single plant is in a long-silique phenotype. The primer can accurately screen the silique length phenotype, significantly improve the selection efficiency and reduce the field workload, thereby accelerating the breeding process.

The invention belongs to the field of crop genetic breeding, and discloses a method for identifying and screening superior germplasm of rape plant canopy leaves and application. The first leaf position of the rape is free of leaves and the uppermost short stalk leaves are used as representative leaves of the canopies of the rape plants from the initial flowering stage to the silique stage. Comprising the following steps: 1) canopy leaf area measurement: measuring the length, width and leaf area of a first leaf position without leaves; 2) performing canopy leaf function duration statistics, recording a development time point and an aging time point when a first leaf position is free of leaves, and obtaining duration when the first leaf position is free of leaves and plays a function; 3) identifying canopy leaf area dominant germplasm, comparing the first leaf position of the to-be-selected material without leaves with the current main planting reference variety, and determining the germplasm with the leafless area exceeding 50% as the leafless area dominant germplasm; 4, canopy leaf function duration dominant germplasms are identified, and materials with the leafless function duration exceeding the current main planting reference variety by 20% or materials with the leafless function duration being 20% or materials with the leafless function duration being 20% or materials with the leafless function duration being 20% or materials with the leafless function duration being 20% or materials with the leafless function duration being 2 weeks before rape plant silique ripening are defined as the leafless function duration dominant germplasms.

The application provides a gene structure variation combination, primers and application thereof significantly related to a Brassica napus growth period, belongs to the field of plant molecular breeding and gene editing technology, and the gene structure variation combination comprises one or more of "DEL00146035 gene structure variation + INS00146036 gene structure variation", DEL00006200 gene structure variation, "DEL00018831 gene structure variation + INS00018834 gene structure variation". BnaC6.MED13 As a major genetic locus (initial flowering time, final flowering time), the gene structure variation combination is respectively composed of BnaA2.GA20ox2 , BnaA4.SPL15 A gene structure variation combination of a genetic locus (silique maturation time) can accurately and quickly and accurately identify a short growth period of a rapeseed variety.

The application discloses rape with regulated silique length BnaCDPK10 Gene and application thereof, and relates to the technical field of plant molecular biology. BnaCDPK10 The nucleotide sequence of the gene is shown as SEQ ID NO. 3, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 4. BnaCDPK10 The application constructs a transgenic overexpression vector of the gene, and obtains a transgenic Arabidopsis thaliana plant overexpressing the gene. BnaCDPK10 It is found that the length of the silique can be obviously increased after the gene is overexpressed, and the gene has the function of positively regulating the length of the silique. The gene is transferred into crops and horticultural plants, and has good application potential in increasing crop yield.

The invention belongs to the technical field of biology, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker related to the pod number of oilseed rape and application of the SNP molecular marker. The SNP molecular marker is located at the 106th site of a nucleotide sequence of SEQ ID NO.1, the SNP molecular marker is closely associated with the silique number of the rape, the SNP molecular marker is not limited by the growth stage of the rape and can be selected in the seedling stage, the breeding workload is greatly reduced, the breeding selection period of the rape is remarkably shortened, the breeding efficiency is improved, and the breeding process is improved.