Molecular marker and application closely related to grain weight and silique length of oilseed rape

A technology of molecular markers and grain weight, which is applied in the field of rapeseed breeding and molecular biology, can solve the problems of low selection efficiency, long breeding cycle, and susceptibility to environmental influences, so as to reduce the workload, shorten the breeding period of rapeseed, and improve the quality of rapeseed breeding. efficiency effect

Active Publication Date: 2018-07-20
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In order to solve the above problems, the present invention provides a molecular marker closely related to rapeseed grain weight and silique length, which solves the problem that grain weight and silique len

Method used

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  • Molecular marker and application closely related to grain weight and silique length of oilseed rape
  • Molecular marker and application closely related to grain weight and silique length of oilseed rape
  • Molecular marker and application closely related to grain weight and silique length of oilseed rape

Examples

Experimental program
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Effect test

Embodiment 1

[0030] A molecular marker BnaA.ARF18a-186 and BnaA.ARF18a-262 closely related to rape seed weight and silique length were obtained by the following method:

[0031] In this example, 157 rapeseed varieties with different genetic sources and large variations in grain weight and silique length were used as an example to construct a natural population, and describe in detail the method for obtaining molecular markers closely related to rapeseed grain weight and silique length. details as follows:

[0032] (1) Group construction:

[0033] A natural population of 157 rapeseed cultivars with different genetic origins and large variations in grain weight and silique length (1.75-5.71g / 4.02-10.62cm) was used as research materials (collected worldwide).

[0034] (2) Determination of grain weight and silique length:

[0035] (1) The plant material is planted in the field, with 3 lines per line, 8-10 plants in each line, random block design; the Yangtze River Basin is generally sown in ...

Embodiment 2

[0061] The application of a molecular marker closely related to rapeseed grain weight and silique length in high-yield breeding of rapeseed comprises the following steps:

[0062] Using the European winter variety Express (low-grain weight silique) and the Chinese semi-winter variety House7 (high-grain weight long silique) as parents, a DH segregation population was constructed as research materials, using BnaA.ARF18a-186 and BnaA.ARF18a -262 Two kinds of dCAPS markers further expand the detection range, the detection method is as follows:

[0063] (1) Extracting DH population and parental DNA, the method is the same as in Example 2.

[0064] (2) First detect the haplotype type of the parents of the DH population, and use the following primers to carry out the PCR process:

[0065] The forward primer sequence of BnaA.ARF18a-186 is 5'-ATGGCGAATGTAGATGGAGAT-3', and the reverse primer sequence is 5'-CTCTGTGTACAGCTGATCTTGATAT-3';

[0066] The forward primer sequence of BnaA.ARF1...

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Abstract

The invention relates to the field of oilseed rape breeding and molecular biology, in particular to a molecular marker and application closely related to the grain weight and silique length of oilseedrape. The molecular marker closely related to the grain weight and silique length of the oilseed rape is characterized by the molecular marker to the molecular marker of gene BnaA.ARF18a, and a nucleotide sequence is shown in SEQ ID No.1. The molecular marker solves the problem that the grain weight and the silique length in a conventional breeding method can be only identified later, so that thebreeding period is long, the breeding is easily affected by the environment, and the selection efficiency is low, the efficiency of the oilseed rape breeding is improved, the grain weight and siliquelength of different breeds of oilseed rape single plants can be quickly and reliably identified in the seedling stage of the oilseed rape, the breeding efficiency of the oilseed rape is improved, andthe breeding cycle of the oilseed rape is greatly shortened.

Description

technical field [0001] The invention relates to the fields of rapeseed breeding and molecular biology, in particular to a molecular marker closely related to rapeseed grain weight and silique length and its application. Background technique [0002] Brassica napus is an important oil crop in my country. It not only provides edible oil for human beings, but also provides feed rich in protein. Therefore, increasing the yield of rapeseed has always been the primary goal of breeders. Seed size (weight) and silique length are important components of rapeseed yield. Hundreds of QTLs for grain weight and silique length have been found in rapeseed in the past two decades, but the genetic and molecular regulatory networks of these QTLs are still unclear. Not sure. [0003] Seed size / weight is considered to be one of the most important traits. As the product of sexual reproduction in higher plants, seeds are not only the most important form of plant reproduction, but also the most im...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 钱伟董红利梅家琴贺亚军傅鹰魏大勇贺艳李玉震
Owner SOUTHWEST UNIVERSITY
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