A05 chromosome major QTL locus of brassica napus major inflorescence silique density character, SNP molecular marker and application

Abstract

The invention provides an A05 chromosome major QTL locus of a cabbage type rape major inflorescence pod density character. The A05 chromosome major QTL locus is located between a 20957390th basic group and a 21315610th basic group of an A05 chromosome of cabbage type rape. Preferably, the contribution rate to the brassica napus main inflorescence silique density character is 15.36%. The SNP molecular marker is closely linked with a first SNP molecular marker, is located at the 20957390th basic group and is A or G, and the mutation causes polymorphism. The SNP molecular marker is closely linkedwith a second SNP molecular marker, is located at the 21315610th basic group and is C or T, and the mutation causes polymorphism. The SNP molecular marker is closely linked with a peak SNP molecularmarker, is located at the 21171737th basic group and is C or T, and the mutation causes polymorphism. The invention further provides a related SNP molecular marker and application. The A05 chromosomemajor QTL locus is high in contribution rate to cabbage type rape main inflorescence pod density traits, plays a key role in adjustment and control of cabbage type rape main inflorescence pod density,can be used for map-based cloning and molecular marker-assisted selection, and is suitable for large-scale popularization and application.

Description

technical field [0001] The invention relates to the technical field of molecular biology and rapeseed breeding, in particular to the technical field of silique density traits of main inflorescence of Brassica napus, and specifically refers to a main effect QTL site and SNP of A05 chromosome for the density trait of siliques of main inflorescence of Brassica napus Molecular markers and their applications. Background technique [0002] The demand for edible oil in my country is growing rigidly at a rate of 1 million tons per year. It is estimated that by 2025, the demand for vegetable oil will be 3.5-4.0 times that of the existing domestic production capacity. However, my country's arable land resources continue to decrease, so increasing the yield of oil crops The fundamental way to increase the total output of oilseeds. Rapeseed is the largest oil crop in my country, with an annual sowing area of ​​more than 100 million mu and a total output of more than 15 million tons. Rap...

AI Technical Summary

Problems solved by technology

Genes controlling seed density have been cloned in crops such as rice and corn, but little research has been done on silique density in rapeseed

Images