Single nucleotide polymorphism (SNP) molecular marker primer of pear fruit juice content main effect quantitative trait locus (QTL) and application thereof

A technology of fruit juice content and labeling primers, applied in the field of molecular genetics and breeding, can solve the problems that the development and application of SNP molecular markers have not yet been reported, and achieve the effects of high linkage and reliability, and improved accuracy and efficiency

Active Publication Date: 2014-09-10
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0004] At present, the research on QTL mapping of quantitative traits of pears is still in its infancy. Existing studies are mainly aimed at some diseases or growth traits, QTL mapping of pear juice content traits and detection of polymorphism expression on QTL loci. The development and application of SNP molecular markers has not been reported yet

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  • Single nucleotide polymorphism (SNP) molecular marker primer of pear fruit juice content main effect quantitative trait locus (QTL) and application thereof
  • Single nucleotide polymorphism (SNP) molecular marker primer of pear fruit juice content main effect quantitative trait locus (QTL) and application thereof
  • Single nucleotide polymorphism (SNP) molecular marker primer of pear fruit juice content main effect quantitative trait locus (QTL) and application thereof

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Embodiment 1

[0029] Molecular markers linked to the main QTL for pear fruit juice content were obtained by the following methods:

[0030] a) Using 'August Red' and 'Dangshansu pear' (variety is known and public, see literature: Zhang Ruiping et al., Construction of pear AFLP marker genetic map and QTL mapping of fruit-related traits, Acta Horticultural Science, 2011, 38(10): 1991–1998) to obtain 102 F 1 Progeny single plant.

[0031] b) Using RADseq method for high-throughput sequencing, analyze 'August Red' and 'Dangshan Suli' and their offspring, and statistically analyze the genetic type of polymorphic sites in the offspring population, using χ 2 The test analyzes whether the segregation of each marker conforms to the Mendelian genetic segregation ratio of 3:1 or 1:1.

[0032] c) Molecular genetic linkage maps of 'August Red' and 'Dangshan Suli' were constructed using Joinmap4.0 analysis software. Import the polymorphic marker sites obtained in step b) into Joinmap4.0 according to t...

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Abstract

The invention provides a single nucleotide polymorphism (SNP) molecular marker primer of pear fruit juice content main effect quantitative trait locus (QTL) and application thereof. A forward primer sequence of a close linkage SNP mark Pyd05_008 of the pear fruit juice content main effect QTL is shown in SEQ ID NO.1 and a reverse primer sequence is shown in SEQ ID NO.2. The content of the fruit juice is identified by utilizing the specific primer based on a high resolution melting curve and can be parted well, so that the difference of the polymorphism expression fruit juice content on qJuice of QTL is determined. The pear fruit juice content main effect QTL molecular marker can be used for marker-assisted selective breeding of the phenotypic trait of the pear fruit juice content; for quickening the genetic improvement process of the pear species, the SNP molecular marker primer has the important theoretical and practical guiding significance in improving the breeding selection efficiency.

Description

technical field [0001] The invention belongs to the field of molecular genetic breeding, and relates to a SNP molecular marker primer for a main effect QTL site of pear fruit juice content and an application thereof. Background technique [0002] my country is one of the most important origins of Pyrus L. in the world, with rich genetic diversity. The cultivars of pears are widely distributed, and they are cultivated except in Hainan Province and Taiwan Province. The pear pulp is crisp, tender and juicy, sweet and sour, and is very popular among consumers. The content of pear juice not only affects the flavor of fresh fruit, but also contains rich nutrients that are beneficial to human health. For example, pear juice is cool in nature and can clear away heat and calm. And tannic acid and other ingredients, can eliminate phlegm and relieve cough, and have a maintenance effect on the throat; pear juice also contains more carbohydrates and vitamins, which are easily absorbed ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张绍铃吴俊李雷廷姚改芳刘伦杨雅楠谢智华
Owner NANJING AGRICULTURAL UNIVERSITY
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