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Main effect QTL site of the main inflorescence pod number character of Brassica napus, development of SNP molecular markers and application thereof

A technology of Brassica napus and molecular markers, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., to achieve the effect of accelerating the process, high contribution rate, and suitable for large-scale popularization and application

Active Publication Date: 2019-10-22
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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Problems solved by technology

Although there have been reports on the QTL of rapeseed silique number and its related traits, most of them are in the initial stage of mapping.

Method used

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  • Main effect QTL site of the main inflorescence pod number character of Brassica napus, development of SNP molecular markers and application thereof
  • Main effect QTL site of the main inflorescence pod number character of Brassica napus, development of SNP molecular markers and application thereof
  • Main effect QTL site of the main inflorescence pod number character of Brassica napus, development of SNP molecular markers and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Determination of the phenotype of the main inflorescence silique number trait of Brassica napus

[0031] 1. Determination of the main inflorescence silique number phenotype of the associated population

[0032] (1) 324 high-generation lines of Brassica napus from all over the world constitute a natural population, and complete the investigation of the number of siliques in the main inflorescence at 2 points in 3 years at the Yangluo Experimental Base of Wuhan City and the Experimental Base of Yangzhou Academy of Agricultural Sciences.

[0033] (2) The seedlings were fixed by direct seeding, the row spacing was 33 cm, the plant spacing was 15 cm, 4 rows in each plot, and three replicates were randomly designed. Protective rows were planted around the test material fields.

[0034] (3) The number of siliques in the main inflorescence: 10 plants were taken from each plot, and the number of siliques containing more than one full or less full seed on the main infl...

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Abstract

The invention provides a main effect QTL site of the main inflorescence pod number character of Brassica napus. The main effect QTL site is located between bases 5808417 and 7114866 in the A02 chromosome of Brassica napus. Preferably, the contribution rate of the main effect QTL site to the main inflorescence pod number of Brassica napus is 10.6%. The site is closely linked to the first SNP molecular marker. The first SNP molecular marker is located at base 5580417, which is C or T. This mutation leads to polymorphism. The site is closely linked to the second SNP molecular marker. The second SNP molecular marker is located at base 7113686, which is A or C. This mutation leads to polymorphism. The site is closely linked to the peak SNP molecular marker. The peak SNP molecular marker is located at base 6926697, which is A or C. This mutation leads to polymorphism. The invention also provides development of related SNP molecular markers and application thereof. The main effect QTL site ofthe main inflorescence pod number character of the Brassica napus provided by the invention has a high contribution rate to the main inflorescence pod number character of the Brassica napus, plays akey role in the regulation of the main inflorescence pod number of the Brassica napus. It can be used for map-based cloning and molecular marker-assisted selection, and is suitable for large-scale promotion and application.

Description

technical field [0001] The invention relates to the technical field of molecular biology and rapeseed breeding, in particular to the technical field of the number traits of the main inflorescence siliques of Brassica napus, in particular to the SNP molecular markers closely linked to the main effect QTL loci of the traits of the number of siliques in the main inflorescence of Brassica napus and application. Background technique [0002] Rapeseed (Brassica napus) is an important oil-bearing crop in the Brassica genus, and napus is the main oil-bearing crop widely planted in the world, and it is also the only winter oil-bearing crop in my country. Therefore, the country regards the development of rapeseed production as the focus of ensuring the safe supply of edible oil, which has important practical significance. Brassica napus in my country has become the main cultivar due to its characteristics of disease resistance, high yield, wide adaptability, and strong retrograde tol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 刘胜毅张园园唐敏强刘越英程晓辉黄军艳童超波董彩华
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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