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Method for distinguishing reservoir fluid type by adopting resistivity data
ActiveCN101930082AMastering Real Response PropertiesImprove the identification rateElectric/magnetic detection for well-loggingMaterial resistancePorosityLower limit
The invention discloses a method for distinguishing a reservoir fluid type by adopting resistivity data, relating to the technological field of petroleum and gas logging, geology and core test analysis. The method comprises the following steps of: a. logging by utilizing core data scales and accurately calculating reservoir shale content, rock constituent and porosity; b. eliminating the influence of rock characters and the porosity on resistivity; c. utilizing a core test to obtain the parameters of m, a, n and b reflecting a pore structure, calculating the resistivity lower limit RR of an air layer and eliminating the influence of the pore structure on the resistivity; and d. distinguishing the reservoir fluid type by comparing a deep-induction and deep- lateral resistivity value RT andthe resistivity lower limit RR of the air layer obtained in the step c. The invention eliminates the influence of non-fluid factors of the rock characters, the porosity, the pore structure and the like on the resistivity, and maintains and utilizes the response characteristics of fluids with different resistivities so as to greatly enhance the coincidence rate for distinguishing the reservoir fluid type.
Owner:BC P INC CHINA NAT PETROLEUM CORP +1
Method for identifying lithology of three-dimensional volcanic rock
The invention relates to a method for identifying the lithology of volcanic rock. The method comprises the following steps of: determining the types of rock needing lithological identification; calculating the content of each element in stratigraphic rock in percentage by weight by using the theory of oxygen closed mold on the basis of elemental capture spectroscopy logging information; classifying the lithology of volcanic rock by using a silicon-alkali classification method; extracting the rock structure information of different classes of lithology of volcanic rock on the basis of imaging logging information in combination with oilfield on-site coring analysis and geologic description; combining the rock components calculated according to elemental capture spectroscopy logging with therock structure information determined according to the imaging logging information together and converting a TAS two-dimensional plane into a three-dimensional plane by using the rock structure information as a third dimension scale; and realizing three-dimensional volcanic rock lithological identification by using an SVM (Support Vector Machine) method. By adopting the method, the coincidence rate of lithological identification can be improved to reach more than 90%, which is 10 times that of the traditional method.
Owner:PETROCHINA CO LTD
Method for discriminating type of reservoir fluid by using resistivity data
InactiveCN102012526AMastering Real Response PropertiesImprove the identification rateElectric/magnetic detection for well-loggingAcoustic wave reradiationLithologyPorosity
The invention discloses a method for discriminating the type of reservoir fluid by using resistivity data, which relates to the technical field of oil and gas logging as well as geology and core experiment analysis, and comprises the steps of: a, logging the well through scaling core data, and correctly calculating the mud content, the rock constituents and the porosity of the reservoir fluid; b, eliminating the influence from the lithology and the porosity to the resistivity; c, obtaining m, a, n and b by using core experiment to reflect the parameters of the pore structure, calculating the lower limit RR of the gas-layer resistivity, and eliminating the influence from the pore structure to the resistivity; and d, discriminating the type of the reservoir fluid through comparing a deep lateral resistivity RT in deep induction with the lower limit RR of the gas-layer resistivity obtained in the step c. The method eliminates the influence from the non-fluid factors such as the lithology, the porosity, and the pore structure and the like to the resistivity, and retains and utilizes the response characteristics of different fluids for the resistivity so as to greatly improve the coincidence rate of the discriminating the type of the reservoir fluid.
Owner:四川西德电器有限公司
A Reservoir Pore Structure Classification Method Based on NMR Three Component Percentages
ActiveCN102297828AHigh compliance rateAnalysis using nuclear magnetic resonancePermeability/surface area analysisPetrologyCoincidence
The invention relates to a method for a classifying pore structure of reservoirs based on percentages of three nuclear-magnetic components. The method comprises the following steps: performing matched nuclear magnetic resonance and mercury injection experiments on rock core samples with different pore textures and classifying the pore textures of the rock core samples on the basis of morphological characteristics of a mercury injection curve; analyzing the change characteristics of the mercury injection curve, a nuclear magnetic resonance T2 spectrum and an accumulated porosity curve of the rock core samples with different pore textures and confirming the key factors controlling the quality of the pore textures; extracting percentages S1, S2 and S3 of small-size, middle-size and large-size pore components in a whole pore system from a nuclear magnetic resonance experimental data; confirming a confirmation standard of the change rule of the sizes of the S1, S2 and S3 of different pore textures; and extracting S1, S2 and S3 from a nuclear-magnetic log data and quickly classifying by utilizing the confirmation standard. The nuclear-magnetic log data of thirty wells are processed by using the method provided by the invention and the coincidence rate between the acquired results and a test oil capacity result is 90%, which is increased by 16% from the coincidence rate acquired by using the prior art.
Owner:PETROCHINA CO LTD
Method for identifying reservoir by combining electrical imaging and reef geologic model
ActiveCN102011583AImprove live interpretationHigh compliance rateCharacter and pattern recognitionBorehole/well accessoriesElectricityWell logging
The invention discloses a method for identifying a reservoir by combining electrical imaging and a reef geologic model, and belongs to the technical field of marine carbonatite well logging interpretation. The method comprises the following steps of: selecting a carbonatite reef reservoir sedimentary facies model according to a reef reservoir sedimentation model; establishing the correspondence between the reef reservoir sedimentation model and an electrical imaging well logging image characteristic based on an electrical imaging well logging image; establishing an electrical imaging image library of a standard reservoir of the carbonatite reef reservoir; performing rock structure characteristic-based image dynamic enhancement contrast according to images in the electrical imaging image library; and determining a part of a reef phase reservoir, through which a well runs, based on a typical image of an electrical imaging reef phase, and comparing with a typical image of a favorable reservoir to determine the quality of the reservoir. The method greatly improves the coincidence rate of well on-site interpretation and test oil, and is fully applied to various related oil fields in China.
Owner:PETROCHINA CO LTD
Log multi-parameter oil-gas interpretation method based on radar map and cloud model
The invention relates to a log multi-parameter oil-gas layer interpretation and evaluation method based on a radar map and a cloud model. The method comprises the steps of: obtaining interpretation and evaluation parameters by normal geological log, gas log, geochemical log, nuclear magnetic log and the like; integrating log interpretation databases; drawing the log multi-parameter oil-gas interpretation radar map according to different reservoir types in different areas by using the databases; and mapping the interpretation parameters onto the rays of the radar map. The radar map is divided into a good reservoir, a medium reservoir and a poor reservoir from inside to outside; the area of the radar map is taken as the characteristic quantity for finishing characteristic extraction; the area of the radar map is used to calculate a cloud boundary equation, and the cloud boundary equation is mapped on the manufactured cloud scale; the cloud model is the same as the radar map, namely the reservoir is divided into a dry layer area, a low-yield oil-gas layer area and an oil-gas layer area with industrial value from inside to outside. The intuitional and quantitative interpretation and evaluation of log multi-parameter oil-gas reservoirs is realized by the radar map and the cloud model. The interpretation coincidence rate can be increased, and the interpretation conclusion is represented by a graph, so that the log multi-parameter oil-gas layer interpretation and evaluation method is intuitional in expression and strong in applicability.
Owner:CHINA PETROLEUM & CHEM CORP +1
Continuous tube horizontal borehole process and device
The invention relates to a horizontal well logging technology with a continuous tube and a device thereof. The technology uses a high-strength and high-tenacity continuous tube as tools to convey down-hole logging instruments so as to ensure that the down-hole instruments perform tests under various horizontal wells. The adopted device comprises a continuous tubing unit, the continuous tube, a wellhead device, connecting joints between the continuous tubes and the down-hole instruments, down-hole logging instruments and tools for the horizontal wellbore, a ground numerical control logging system, a depth system of the continuous tube and a signal transmission system of the continuous tube. The invention can ensure the down-hole instruments to perform tests under various horizontal holes by using the continuous tube as the conveyance tool of the down-hole instruments; the technology uses few application equipment and has the advantages of good maneuverability, convenient operation and high construction efficiency thanks to the application of continuous tubing unit and other special devices.
Owner:BC P INC CHINA NAT PETROLEUM CORP +1
Double-antibody sandwich ELISA (enzyme linked immunosorbent assay) kit used for detecting avian leukosis group specific antigen
ActiveCN102636644ASensitiveHigh compliance rateImmunoglobulins against virusesMicroorganism based processesAvian leukosis virusesElisa method
The invention discloses double-antibody sandwich ELISA (enzyme linked immunosorbent assay) kit used for detecting an avian leukosis group specific antigen. The kit comprises an enzyme plate coated by a monoclonal antibody which is secreted by a hybridoma cell strain the preservation serial number of which is CGMCC (china general microbiological culture collection center) No.5961. The invention also discloses a double-antibody sandwich ELISA method which is established by utilizing the monoclonal antibody and is capable of rapidly and effectively detecting an ALV (avian leukosis virus). In the double-antibody sandwich ELISA method, the monoclonal antibody prepared by pronucleus expressive HLJ09mdj-1p27 albumen is utilized as a peridium antibody, and antibodies prepared by p27 are utilized as a detection antibody. According to the invention, the minimum detection amount of the p27 is 1.25 ng / ml, the method is not reacted with the common virus of birds, and the specificity is good. The method is utilized to detect egg white and an anus swab sample, and the coincidence rate is respectively 96.5% and 88.9% compared with a PCR (polymerase chain reaction) method; and the result proves that the method has the advantages of convenience, celerity, differentia, sensitivity and the like, and is useful for the detection and population purification of the ALV.
Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Four-quadrant reservoir stratum type identification method and system
ActiveCN103306671AHigh compliance rateAccurately reflectBorehole/well accessoriesMaterial analysis by transmitting radiationElectricityReservoir type
The invention discloses a four-quadrant reservoir stratum type identification method and system, wherein the four-quadrant reservoir stratum type identification method comprises the following steps: selecting a core sample, and acquiring the mud filtrate specific resistance and electrical imaging logging information of a well corresponding to the core sample; performing CT scanning imaging on the core sample to generate a CT imaging pore distribution spectrum of the core sample; according to the mud filtrate specific resistance and the imaging logging pixel conductivity, computing an electrical imaging porosity spectrum of the well corresponding to the core sample by utilizing the Archie's formulas; calibrating the electrical imaging porosity spectrum corresponding to a stratum segment by utilizing the CT imaging pore distribution spectrum, and computing the mean value and distribution variance of the calibrated electrical imaging porosity spectrum; performing standardized treatment on the mean value and distribution variance of the calibrated electrical imaging porosity spectrum to generate the standard mean value and distribution variance of porosities; forming a two-dimension plane by the standard mean value and distribution variance of porosities, wherein the standard mean value of porosities is the abscissa axis, and the standard distribution variance of porosities is the ordinate axis; and identifying the reservoir stratum type according to the two-dimension plane.
Owner:PETROCHINA CO LTD
Method for judging fluid type of reservoir by utilizing logging data of while-drilling drilling fluid
ActiveCN103806911AEasy to masterData sources are timelyBorehole/well accessoriesWell drillingReservoir fluid
The invention discloses a method for judging a fluid type of a reservoir by utilizing logging data of while-drilling drilling fluid, which comprises the following steps of: A, data collection, i.e., collecting performance parameters of the while-drilling drilling fluid of a target stratum, wherein the performance parameters comprise electrical conductivity, a temperature, drilling time and a hydrocarbon component of the drilling fluid; B, data processing; C, setting of a crossplot graph data track, i.e., for a plumb shaft, respectively combining an Sf data track and a Tf data track to form an S-T data track, combining the Sf data track and a DHf data track to form an S-DH data track and respectively making crossplots; D, judgment of the fluid type, i.e., using the S-T dat track for judging a dry layer (a micro gas-bearing bed) and an aqueous layer, using the S-DH data track for the plumb shaft to judge the gas-bearing bed or a gas reservoir and using a DO-DH data track for judging an oil-bearing formation or the gas-bearing bed. When the method is adopted, an explanation result of the method is objective and accurate, the coincidence rate of the explanation result and the oil testing conclusion is high, and the requirements on production and research can be met.
Owner:BC P INC CHINA NAT PETROLEUM CORP +1
Injection-production relation optimizing and adjusting method for well group
InactiveCN104453834ATuning parameters are reliableHigh compliance rateFluid removalSpecial data processing applicationsDecision-makingSaturation distribution
Owner:CHINA PETROLEUM & CHEM CORP +1
Fluorescence immunochromatographic assay method for quantitatively detecting heart fatty acid binding protein and kit for quantitatively detecting same
ActiveCN102520194ASolve the backgroundSolve the signal indistinguishableBiological testingBlood plasmaBiology
The invention discloses a fluorescence immunochromatographic assay method for quantitatively detecting hFABP (heart fatty acid binding protein) and a kit for quantitatively detecting the same. The fluorescence immunochromatographic assay method for quantitatively detecting the hFABP realizes quantitative fluorescence detection on the basis of optimizing components of a test strip by the aid of excellent fluorescent characteristics of quantum dots and by means of combining bicolor labeling technique and immunochromatographic assay. Compared with a conventional colloidal gold immunochromatographic assay method, the fluorescence immunochromatographic assay method has the advantages of fine labeling stability, low non-specificity, high sensitivity, wide linear range and accuracy in quantization. The kit is used for quantitatively detecting the hFABP, can be used for simultaneously detecting whole blood, blood serum and plasma samples, serves as a simple, accurate, specific and inexpensive detecting tool for early screening and prognosis evaluation of acute myocardial infarction, is applicable to hospitals at all levels, and is particularly beneficial to wide popularization in primary hospitals and clinics.
Owner:SHENZHEN KANGMEI BIOTECH
Method for discriminating reservoir fluid by establishing gas logging chart on basis of discriminant analysis
ActiveCN102900433ASolve the problem of difficult to effectively distinguish reservoir fluidsAccurate discriminationBorehole/well accessoriesMarking outPeak value
The invention discloses a method for discriminating reservoir fluid by establishing a gas logging chart on the basis of discriminant analysis, relating to the technical field of oil and gas exploitation and development. The method includes the steps of: a, collecting the gas logging peak value data and the oil test results in an oil test completion well; b, according to the categories of the oil test results, grouping the gas logging peak value data of the logging display sections which have the same category of oil test results; c, establishing discrimination factors by taking the gas logging peak value data of the logging display sections as a base; d, selecting the established discrimination factors, and formulating discrimination functions which can effectively discriminate the property of the fluid on the basis of discriminant analysis; and e, according to the coincidence rates of the formulated discrimination functions, preferentially selecting two discrimination functions with the highest coincidence rates to be used as x axis and y axis to establish a gas logging chart to discriminate the property of the reservoir fluid. The method disclosed by the invention solves the problem that conventional gas logging charts are difficult for effectively discriminating the reservoir fluid, so the dominant regions of different fluids can be well marked out.
Owner:BC P INC CHINA NAT PETROLEUM CORP +1
Colloidal gold test strip and test strip card for detecting IgM antibody, and preparation and detection method
InactiveCN105259345AThe detection process is fastImprove efficiencyBiological testingParainfluenza virus antigenIgm antibody
The invention provides a colloidal gold test strip for detecting an IgM antibody. The IgM antibody is a specific IgM antibody for nine respiratory tract infection pathogens, and the colloidal gold test strip comprises a sample pad, a conjugate pad, a nitrocellulose film and a water absorption pad which are attached to a polyvinyl chloride base plate in sequence; the conjugate pad is a glass fiber film wrapped with a rabbit-anti-human IgM antibody-colloidal gold conjugate; the nitrocellulose film is wrapped with 9 detection lines and 1 quality control line in sequence; the 9 detection lines are respectively a mycoplasma pneumoniae recombined antigen, a chlamydia pneumoniae recombined antigen, an influenza a virus antigen, an influenza B virus antigen, a sendai virus antigen, a legionella pneumophila antigen, a Coxiella burnetii antigen, a respiratory syncytial virus antigen and an adenovirus antigen, and the quality control line is a second antibody. The invention further provides a colloidal gold test strip card comprising the colloidal gold test strip and a colloid gold kit, a preparation method of the colloidal gold test strip card, and a method for realizing detection by adopting the colloidal gold test strip, the test strip card or the kit.
Owner:THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
Colloidal gold immunochromatography test strip used for testing H1N1 influenza antigen and method for testing H1N1 influenza antigen
ActiveCN104101706ARead results quickly and intuitivelyGet rid of dependenceBiological material analysisAntibody conjugateTest strips
The invention relates to a colloidal gold immunochromatography test strip used for testing an H1N1 influenza antigen and a method for testing the H1N1 influenza antigen. The test strip comprises a base plate, a sample pad, a conjugate pad, a pyroxylin film and an absorbent paper, wherein the sample pad, the conjugate pad, the pyroxylin film and the absorbent paper are lapped and adhered on the base plate sequentially; the conjugate pad is a combination of a gold-label-biotin conjugate pad and a streptavidin-gold-antibody conjugate pad; according to the method for testing the H1N1 influenza antigen, a sample to be tested is dropwise added on the test strip; if a red band is displayed at a testing line (1), the sample to be tested contains the H1N1 influenza antigen. According to the advantages, the test strip provided by the invention is cheap in price, fast, simple, convenient, and greatly suitable for on-site testing; moreover, the test strip can meet the requirement of higher testing sensitivity, and has an important clinical diagnostic significance.
Owner:THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
On-site detection immuno-chip and preparation method thereof and application
The invention discloses an on-site detection immuno-chip, comprising a chip carrier and an agarose gel layer which is paved on the chip carrier, wherein, the agarose gel layer is fixed with a plurality of antibody microarrays of 4*4, and chip-dedicated fences or Super PAP Pen scribings are used for separating the microarrays from each other; the double antibody sandwich principle is adopted to detect the antigen to carry out the dot matrix of corresponding capture antibody, positive control and negative control on the solid phase carrier simultaneously; the antibody protein is connected with the solid phase carrier through the covalent bond and physical adsorption; the sample liquid to be detected and the chip are directly incubated; the antigen to be detected in the samples combine with the corresponding antibody which is fixed on the chip; a specific monoclonal antibody probe marked by horse radish peroxidase is added and the macroscopic detection results can be obtained after the coloration of substrates. The invention can detect viruses in multiple aquatic animal samples and the results are macroscopic, thus being applicable to rapid and accurate detection of viruses of aquatic animals in breeding production.
Owner:OCEAN UNIV OF CHINA
Colloidal gold chromatographic band for joint detecting specific IgM. IgG antibody and producing method thereof
The invention provides a colloidal gold chromatography band and the preparing technology to detect the special IgM,IgG. The colloidal gold is as the labeled thing of the colloidal gold. One end of the PVC back plate is pasted with the sample pad, the compound pad, the nitryl fibrous membrane, the other end is pasted with the absorbing pad; the compound pad is the glass fibrous membrane covered with the antigen-colloidal gold compound. The character is in that: the fibrous membrane includes three cover lines which cover the anti IgM antibody, the special antigen and the antibody according to the antigen separately. The invention detects the IgM antibody using the immune capture method principle and detects the IgG antibody by the double antigen sandwich method. So it can detect the special IgM, IgG antibody by one operation and the result has the high whole according ratio.
Owner:北京英诺特生物技术股份有限公司
ELISA (enzyme linked immunosorbent assay) kit for detecting PPRV (peste des petits ruminants virus) antibody
ActiveCN107238702AEasy to monitorStrong specificitySsRNA viruses negative-senseAntibody mimetics/scaffoldsAntigenElisa kit
The invention discloses an ELISA (enzyme linked immunosorbent assay) kit for detecting a PPRV (peste des petits ruminants virus) antibody. The ELISA kit for detecting the PPRV antibody comprises an envelope antigen, wherein the envelope antigen is a recombinant PPRV H-F fusion protein which is a protein of a) or b) as follows: a) protein formed by an amino acid sequence shown in SEQ ID No.2; b) soluble protein obtained from the amino acid sequence shown in SEQ ID No.2 through substitution and / or deletion and / or addition of one or several amino acid residues. The ELISA kit for detecting the PPRV antibody not only can be used for diagnosing PPR (peste des petits ruminants), but also can be used for evaluating a vaccine immunity effect, further can detect PPR rapidly and accurately and is beneficial to clinical monitoring of PPR, thereby playing a positive role in better control of spread of PPR in China.
Owner:CHINA ANIMAL DISEASE CONTROL CENT
Dot-immunogold filtration reagent kit for livestock schistosomiasis diagnosis, its preparation and application method
InactiveCN1700008ACheap manufacturingLow costMaterial analysis by observing effect on chemical indicatorBiological testingAntigenFiltration
The invention provides a preparing and application method of diffusion medium box for diagnosing livestock's snail fever spot gold, which is a new immune labeling technique and livestock's snail fever immunology diagnosis technique. The invention labels colloid gold onto Japanese snail antigen protein, and is suit for testing many kinds of animals such as cattle, sheep, pig and rabbit; it spots tested animal's blood dried paper leachate onto pyroxylin film as posited phase, then it droplets snail antibody colloid gold 2í½3 drops (100í½150ª–l) and produces naked eye visible red speckle in 2 minutes. Its positive coincidence rate is 100% and its negative coincidence rate is 99.2%.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Colour development protein chip using BCIP/NBT as substrate as well as application in detection of autoantibody thereof
InactiveCN101226192AIncreased sensitivityImprove featuresMaterial analysis by observing effect on chemical indicatorBiological testingAntigenAutoantibody production
The invention discloses a color protein chip which substrate is BCIP / NBT. The invention is composed of a substrate, an array, 12 antigens coated on the array, negative contrast, positive contrast and blank contrast, wherein the substrate is a glass slide decorated by APES, the kinds and point sample densities of the 12 antigens are 520ug / ml ANA, 465ug / ml Ro-60, 530ug / ml La / SSb, 530ug / ml Jo-1, 525ug / ml Scl-70, 520ug / ml Sm, 615ug / ml Ro-52 / SSa, 340ug / ml RF, 465ug / ml CCP, 410ug / ml u1RNP, 490ug / ml CENP-B, 580ug / ml dsDNA. The invention uses the alkali phosphatase coloring method whose substrate is BCIP / NBT as the final check signal of protein chip to be widely used for self antibody check, while the check sensitivity has higher coincidence rate than prior art, with reduced check cost and time. The invention can be used in clinical check, sanitary supervision, regular sanitary supervision and scientific research or the like.
Owner:SHANDONG MEDICAL BIO TECH RES CENT
Oligonucleotide chip capable of detecting five enteroviruses simultaneously and application thereof
InactiveCN101654713AStrong specificityHigh compliance rateNucleotide librariesMicrobiological testing/measurementFluorescenceOligonucleotide chip
The invention discloses an oligonucleotide chip capable of detecting five enteroviruses simultaneously and an application thereof. The chip comprises a substrate, probes of pathogens of five enteroviruses coated on the substrate, negative contrast, positive contrast and blank contrast, wherein the probes of pathogens are five 54-70mer of HAV-P, ROV-P, NOV-P, ASV-P and ADV-PV1 probes. The chip of the invention adopts multiple PCR to amplify a plurality of target sequences of pathogens simultaneously and uses the downstream primer Tamara labeling method to perform fluorescence labeling to PCR product, the labeled PCR product is hybridized with the chip to realize the accurate detection of pathogens, the detection sensitivity is equal to that of PCR and the specificity is high. The detectionefficiency and accuracy are obviously shortened. The technology system of the invention is applicable to fields such as sea water and marine life specimens monitoring, food hygiene surveillance, customs quarantine, related clinical detection and the like.
Owner:SHANDONG MEDICAL BIO TECH RES CENT +1
Aspergillus galactomannan (GM) antigen immunodetection kit as well as preparation method and application thereof
InactiveCN105866407ASensitive detectionIncreased sensitivityBiological material analysisColor depthCoincidence
The invention provides an Aspergillus galactomannan (GM) antigen immunoassay kit, comprising a solid phase carrier coated with GM antigen, an anti-GM antigen polyclonal antibody and a GM antigen standard product, the kit first GM antigen is coated on a solid phase carrier. During detection, the sample to be tested or GM antigen standard competes with the coated antigen for limited antibody binding sites, and then a color reaction occurs between the enzyme and the substrate, and the color depth of the measurement result It is negatively correlated with the concentration of the antigen to be tested. A standard curve can be drawn according to the GM antigen standard, and the concentration of the antigen to be tested can be calculated according to the standard curve equation. The kit has good sensitivity and specificity, the result coincides with the reference reagent in a high rate, and can provide more accurate and reliable test results. Its operation is simple and easy, and the detection is fast and sensitive. Inexpensive, the detection kit provides an effective tool for the quantitative detection of GM antigen.
Owner:DYNAMIKER BIOTECH TIANJIN
Joint inspection kit for IgG and IgM antibodies of hepatitis C virus and preparation method thereof
InactiveCN102109523AQuick screeningEasy to operateMaterial analysis by observing effect on chemical indicatorGlass fiberNitrocellulose
The invention relates to a joint inspection kit for IgG and IgM antibodies of hepatitis C virus and a preparation method thereof. The kit adopts colloidal gold as markers; a sample pad (1), a colloidal gold pad (2), a nitrocellulose membrane (3) and a sample absorbing pad (4) are mutually overlapped and adhered on a bottom board (5) in sequence, wherein the colloidal gold pad (2) is made of glass fibers or non-woven fabrics with mixed antigens of the hepatitis C virus (HCV) marked by colloidal gold; and the nitrocellulose membrane (3) is coated by anti-human IgM antibodies and HCV mixed antigens respectively as detecting lines and is coated with anti-HCV antibodies as quality control lines. In the invention, a colloidal gold immunochromatography technology is adopted to determine the IgG and IgM antibodies of hepatitis C, and the IgG and IgM antibodies of the hepatitis C can be jointly inspected by one-time operation, so that the operation process is simplified. The invention has the characteristics that the operation is simple, the reaction is fast, the sensitivity is high and the economy and practicability are achieved and the like.
Owner:北京库尔科技有限公司
Recognition method for underground single ancient channel under condition of dense well pattern
ActiveCN106837319AAvoid drudgeryAvoiding Insufficient Determination of Vertical ResolutionGeological measurementsBorehole/well accessoriesImage resolutionModel fitting
The invention discloses a recognition method for an underground single ancient channel under the condition of a dense well pattern. By adoption of an analogy method, field outcrop observation results are used for guiding recognition of the underground single ancient channel, and a model fitting geological thinking method is embodied. Through application of the recognition method in a Sulige gas field, quantitative description results are applied to three-dimensional geological modeling and serve as constraint conditions to enable the model consistency rate to be remarkably increased. According to the recognition method for the underground single ancient channel under the condition of the dense well pattern, aiming at morphological characteristics of the single ancient channel, dense well pattern drilling data are sufficiently utilized, tedious work of sedimentary micro-phase interpretation conducted on a single well is avoided, the phenomenon that the seismic datum longitudinal resolution ratio is not enough for determining is avoided, and the recognition method has the characteristic of being convenient to operate. According to application of the recognition method in the Sulige gas field, recognition accuracy of vertical sand bodies is improved to be less than 8 m from being 15-20 m, the original quantitative description results achieve quantification, the results are applied to three-dimensional geological modeling and serve as the constraint conditions to enable the model consistency rate to be increased by 10%, and the effect is obvious.
Owner:PETROCHINA CO LTD
Chemiluminescence detection kit of swine foot-and-mouth disease 3ABC and 2C antibodies
ActiveCN106596932AHigh purityDetection of high sensitivityChemiluminescene/bioluminescenceEscherichia coliSerum ige
The invention discloses a chemiluminescence detection kit of swine foot-and-mouth disease 3ABC and 2C antibodies and belongs to the field of immunological detection. The kit comprises a chemiluminescent immunoreaction plate, an enzyme-labeled antibody, a serum diluent, a chemiluminescent substrate, a chemiluminescent enhancer and a PBST washing liquid, and is characterized in that the chemiluminescent immunoreaction plate is a milky white opaque polystyrene 96-well plate, the bottom in each one of the wells is coated with a 3ABC-2C fusion protein, the enzyme-labeled antibody is a HRP-rabbit anti-pig lgG antibody, the serum diluent comprises Tween-20, bovine serum albumin and a lysate of escherichia coli, the chemiluminescent substrate comprises luminol and bovine serum albumin, and the chemiluminescent enhancer comprises IPP, H2O2 and Tween-20. Compared with CLIA only coated with a 3BAC antibody and the commercial common ELISA kit, the chemiluminescence detection kit has higher sensitivity, specificity and diagnostic ability and good repeatability and stability.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Oligonucleotide chip and application thereof
InactiveCN101586149ASuitable for clinical testing applicationsStrong specificityMicrobiological testing/measurementPositive controlOligonucleotide chip
The invention discloses an oligonucleotide chip labeling a random primer PCR amplification based on a biotin-avidin-cy3 reaction system, which is made by a substrate, and CMV, HSVI, RV, EBV or TB pathogen probes, a negative control, a positive control and a blank control that are coated on the substrate in an array way, wherein the substrate is a surface aldehyde glass slide; the pathogen probes are 10 of CMV-1, CMV-2, HSVI-1, HSVI-2, RV1, RV2, EBV1, EBV2, TB1 and TB2 probes with 70mer; the positive control is a synthesized marker probe; and the blank control is double distilled water. The invention performs the biotin-11-dUTP amplified labeling to various pathogen target sequences by using the random primer and detects the pathogen by adopting a method of increasing the labeling efficiency through using a biotin-avidin-cy3 signal amplification system, has high detection sensitivity and obviously shortens the detection cost and the detection time. The technical system can be suitable to the fields of clinical detection, health supervision, customs quarantine control, scientific research, and the like.
Owner:SHANDONG MEDICAL BIO TECH RES CENT
Detection method for specific antibodies IgM of mycoplasma pneumonia and influenza viruses based on micro-fluidic chip
ActiveCN104360060AAvoid non-specific reactionsMeet testing needsChemiluminescene/bioluminescenceBiological material analysisSmall sampleSpecific antibody
The invention relates to a detection method for specific antibodies IgM of mycoplasma pneumonia and influenza viruses based on a micro-fluidic chip and application of the detection method. The method is characterized by taking the micro-fluidic chip with a plurality of pipelines as a reaction platform and detecting by using a chemical luminescent signal so as to independently or simultaneously detect the serum specific antibodies IgM of mycoplasma pneumonia and influenza viruses. As the micro-fluidic chip is combined with chemiluminiscence, the detection method integrates the advantages of rapidness, high efficiency and small sample amount of the micro-fluidic chip and high specificity of chemiluminiscence, also has the advantages of simplicity in operation, low cost, rapidness and the like, is a multi-target, real-time and high-sensitivity detection method and can be applied to rapid diagnosis of pneumonia and influenza.
Owner:THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
Method for identifying properties of reservoir fluid by three-dimensional quantitative fluorescence parameter charts
InactiveCN104100262AImprove the level ofHigh compliance rateBorehole/well accessoriesAnalysis dataReservoir fluid
The invention discloses a method for identifying properties of reservoir fluid by three-dimensional quantitative fluorescence parameter charts. The method includes the steps of based on tested sidewall core quantitative fluorescence analysis data, establishing an interpretation chart, where the properties of the reservoir fluid are identified through three-dimensional quantitative fluorescence parameters, in a two-dimensional space composed of oiliness indexes and comparison grades; collecting samples to be identified, and analyzing the samples to obtain three-dimensional quantitative fluorescence spectrogram data of the samples to be identified; projecting the oiliness indexes and the comparison grades in the obtained three-dimensional quantitative fluorescence spectrogram data to the interpretation chart, where the properties of the reservoir fluid are identified through three-dimensional quantitative fluorescence parameters; judging the properties of the reservoir fluid according to an area of projection points. After the method is applied to Bohai Oilfield, interpretation coincidence rate reaches 89.5%, and the level and coincidence rate of comprehensive interpretation for fluid properties on a logging site are greatly increased. The method is simple and practical, is easy to operate, allows timely judgment, and has great application and popularization prospects.
Owner:CHINA NAT OFFSHORE OIL CORP +1
Evaluation method for judging water-oil-gas distribution in petroleum and natural gas reservoir stratum
The invention discloses an evaluation method for judging water-oil-gas distribution in a petroleum and natural gas reservoir stratum. A novel gas logging and gas interpretation and evaluation method which has certain application range and is prior to a Pixler method in the judgment of a gas reservoir, an oil reservoir, a water-oil reservoir and a water reservoir of the reservoir stratum is summarized according to three gas ratios, namely C2 / C1 (the ratio of ethane to methane), C3 / C1 (the ratio of propane to methane) and C4 / C1 (the ratio of butane to methane) by using statistic analysis of field gas logging and well completion oil testing data of a petroleum and natural gas exploration and development well. The evaluation method has the advantages of simpleness, practicality, higher coincidence rate and wide application range. Particularly, the evaluation method has unique technical advantages of quickly and accurately obtaining the characteristics of water, oil and gas contained in an underground reservoir stratum by drilling in the logging while drilling operation process of petroleum and natural gas exploration and development.
Owner:CNPC BOHAI DRILLING ENG
Method for recognizing reservoir fluid property by adopting three-dimensional quantitative fluorescent longitudinal parametric variation trend
InactiveCN104076020AImprove the level ofHigh compliance rateFluorescence/phosphorescenceContrast levelWell logging
The invention discloses a method for recognizing reservoir fluid properties by adopting a three-dimensional quantitative fluorescent longitudinal parametric variation trend. The method comprises the following steps: comparing and analyzing oiliness concentration and contrast level data in collected three-dimensional quantitative fluorogram data and a corresponding oil testing conclusion and a well logging interpretation result and establishing the interpretation standards of judging the reservoir fluid properties; collecting and analyzing specimens to be recognized to obtain the three-dimensional quantitative fluorogram data of the specimens to be recognized; determining the reservoir fluid properties by contrasting the oiliness concentration and the comparison level in the three-dimensional quantitative fluorogram data of the specimens to be recognized with the interpretation standards on the basis of the variation trend of well depths. According to the invention, when the method is applied in the Bohai oilfield, the interpretation coincidence rate can reach 89.5%, and the level and the coincidence rate of the comprehensive interpretation fluid properties in well logging fields can be greatly improved; the method is simple, practical, easy to operate and timely and accurate in judgment, and has a very good application and popularization prospect.
Owner:CHINA NAT OFFSHORE OIL CORP +1
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