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Joint inspection kit for IgG and IgM antibodies of hepatitis C virus and preparation method thereof

A hepatitis C virus and kit technology, which is applied in the direction of material analysis, measuring device, instrument, etc. by observing the impact on chemical indicators, can solve the problems of cumbersome operation process, insufficient detection convenience and speed, and simplify the operation process. , rapid response, high sensitivity effect

Inactive Publication Date: 2011-06-29
北京库尔科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the detection of hepatitis C virus IgG antibody and IgM antibody separately, and then comprehensive analysis of the test results has no effect on the diagnosis of the disease, but the operation process is cumbersome and the detection is not convenient and fast enough.

Method used

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  • Joint inspection kit for IgG and IgM antibodies of hepatitis C virus and preparation method thereof
  • Joint inspection kit for IgG and IgM antibodies of hepatitis C virus and preparation method thereof
  • Joint inspection kit for IgG and IgM antibodies of hepatitis C virus and preparation method thereof

Examples

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Embodiment 1

[0034] Example 1: Preparation of Hepatitis C Virus IgG and IgM Antibody Joint Inspection Kit

[0035] 1. Preparation of HCV multi-epitope complex antigen

[0036] Five highly conserved T cell and B cell epitopes of hepatitis C virus were selected from the core protein (1-16aa, 132-141aa), E1 (126-135aa), NS3 (139-147aa) and NS5 (768- 775aa), the hepatitis C virus multi-epitope complex antigen was prepared by E. coli expression and high purification.

[0037] 2. Preparation of anti-human IgM antibodies

[0038] Purified high-concentration human IgM was used to immunize BALB / C mice. The spleen cells of the immunized mice were fused with mouse myeloma cells. The antibody-positive well cells were cloned three times and then injected into the abdominal cavity of BALB / C mice to prepare ascites. , Purify the monoclonal antibody by the octanoic acid method. Store at 0-4°C and put in the middle for later use.

[0039] 3. Preparation of colloidal gold particles

[0040] Take 100ml of 0.01% chlo...

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Abstract

The invention relates to a joint inspection kit for IgG and IgM antibodies of hepatitis C virus and a preparation method thereof. The kit adopts colloidal gold as markers; a sample pad (1), a colloidal gold pad (2), a nitrocellulose membrane (3) and a sample absorbing pad (4) are mutually overlapped and adhered on a bottom board (5) in sequence, wherein the colloidal gold pad (2) is made of glass fibers or non-woven fabrics with mixed antigens of the hepatitis C virus (HCV) marked by colloidal gold; and the nitrocellulose membrane (3) is coated by anti-human IgM antibodies and HCV mixed antigens respectively as detecting lines and is coated with anti-HCV antibodies as quality control lines. In the invention, a colloidal gold immunochromatography technology is adopted to determine the IgG and IgM antibodies of hepatitis C, and the IgG and IgM antibodies of the hepatitis C can be jointly inspected by one-time operation, so that the operation process is simplified. The invention has the characteristics that the operation is simple, the reaction is fast, the sensitivity is high and the economy and practicability are achieved and the like.

Description

Technical field [0001] The invention belongs to the technical field of biological applications, and particularly relates to a hepatitis C virus IgG and IgM antibody joint inspection kit and a preparation method thereof. Background technique [0002] Hepatitis C virus is abbreviated as HCV and belongs to positive-strand RNA virus in virus classification. It has a spherical shape with a diameter of less than 80nm (36-40nm in liver cells, 36-62nm in blood). Hepatitis C is caused by HCV and is one of the infectious diseases that seriously threaten human health. Since there is no preventive vaccine and no specific drug treatment, early diagnosis is an effective means to prevent the spread of HCV. Hepatitis C is caused by infections such as blood transfusion or blood products, hemodialysis, apheresis and blood cell transfusion, kidney transplantation, intravenous drug use, sexual transmission, mother-to-child transmission, etc. In the prevention of hepatitis C, screening of blood dono...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/576G01N21/78
Inventor 李峰陈立柱杨利
Owner 北京库尔科技有限公司
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