The invention belongs to the technical field of medicines, and relates to honokiol nanoparticles and a preparation method thereof. The honokiol nanoparticles are prepared by adopting an ultrasonic-precipitation method in combination with a freeze-drying technology, and the composition of prescription is as follows: the combination ratio of honokiol to a stabilizer is 1:(0.05-50). According to the honokiol nanoparticles and the preparation method thereof, through prescription optimization, povidone (PVP) and bovine serum albumin (BSA) are screened as the best stabilizer combination. The prepared honokiol nanoparticles are high in drug loading, stable in nature, simple in prescription composition, easy, convenient and feasible in preparation process, and the particle size range is 100nm-300nm; and through the preparation of the nanoparticles, the dissolution of honokiol can be obviously promoted, the oral bioavailability is improved, and tissue distribution in the body is improved. In addition, the honokiol nanoparticles can be freeze-dried, and a suitable excipient is added into the obtained freeze-dried powder for further preparing different dosage forms such as oral liquid, tablets, granules and capsules, and also can be prepared into injection for subcutaneous, muscle or intravenous drug delivery so as to be convenient for clinical application.