The application discloses a method for determining human bocavirus type I / II based on HNB-LAMP and
CRISPR / Cas12a-AuNP double colorimetric signals, and belongs to the technical field of
in vitro molecular diagnosis. The application first performs isothermal amplification on a human bocavirus NP1
gene in a
single tube by using HNB-LAMP technology, realizes one-step visual preliminary screening through color change (purple to blue) of hydroxynaphthol blue (HNB), then adds a
CRISPR / Cas12a-AuNP
typing system to positive amplification products, utilizes trans-cleavage of HT44 hairpin transduction structure after Cas12a is activated by specific amplicons, releases
RNA cross-linking agent, and then causes aggregation and
precipitation of
DNA functionalized gold nanoparticles (AuNPs), to produce visible purple precipitate. The application realizes accurate
typing detection of human bocavirus type I / II through double colorimetric signals (blue solution + purple precipitate), does not need complex instruments, is simple and easy to operate, has high sensitivity and strong specificity, and is suitable for primary medical units and on-site rapid screening.