35 results about "Pepsinogen I" patented technology

The invention relates to a kit for detecting pepsinogen I and a preparation method of the kit, and relates to the technical field of biology. The kit comprises an enzyme marker, a luminous marker, a luminous auxiliary agent and a triggering agent, the enzyme marker comprises an anti-PG I monoclonal antibody marked by horse radish peroxidase; the luminous marker comprises an anti-PG I monoclonal antibody marked by 9, 10-dihydroacridine; the light-emitting auxiliary agent comprises H2O2. According to the present invention, the spatial proximity chemiluminescence analysis method is adopted, and the carrier and the washing process are not required, such that the kit cost is reduced, and the detection time is shortened; the method adopts homogeneous reaction, so that the detection result is more real and credible.

The invention provides a pepsinogen II determination kit and a quantitative determination method, the kit comprises a reagent R1 and a reagent R2, the reagent R1 comprises a luminous donor molecule labeled pepsinogen II first antibody and a reaction buffer solution; and the R2 reagent comprises a light-emitting receptor molecule labeled pepsinogen II second antibody and a reaction buffer solution. A double-antibody sandwich structure marked by a luminescent donor and a luminescent receptor respectively is adopted, binding and luminescence can be completed in a liquid phase, solid phase coating and subsequent multi-step washing are not needed, real homogeneous detection and one-step incubation are achieved, the operation process is simplified, the detection time is shortened, and a full-automatic platform is adapted; and an improved buffer system can inhibit non-specific adsorption and reduce heterotropism interference such as RF / HAMA, so that the detection accuracy and repeatability are improved.

The invention belongs to the technical field of antibodies, and particularly relates to an anti-human pepsinogen I (Pesinogen I, PG I) antibody, an antibody pair and a detection kit. The antibody is a first antibody or a second antibody, amino acid sequences of light chains CDR1-3 of the first antibody are respectively shown as SEQ ID NO.3-5, and amino acid sequences of heavy chains CDR1-3 of the first antibody are respectively shown as SEQ ID NO.8-10; the amino acid sequences of light chains CDR1-3 of the second antibody are respectively as shown in SEQ ID NO.13-15, and the amino acid sequences of heavy chains CDR1-3 of the second antibody are respectively as shown in SEQ ID NO.18-20. The antibody and the antibody pair disclosed by the invention have specific recognition and binding capacity and relatively high affinity and detection sensitivity to human PG I protein, and a reliable antibody tool and a detection means are provided for stably detecting trace-level human PG I protein.

The invention discloses a kit and a method for detecting pepsin based on polypeptide, and belongs to the technical field of proteomics. The amino acid sequence of the polypeptide is as shown in SEQ ID No. 1. The polypeptide is obtained based on enzymolysis of pepsinogen PGA4 and screening, the polypeptide and isotope labeled polypeptide are utilized to establish a standard curve, a biological sample is further subjected to enzymolysis to obtain a peptide fragment sample, and pepsin in the biological sample can be quantified by detecting the content of the polypeptide in the peptide fragment sample. By utilizing the method disclosed by the invention, the pepsin in the biological sample can be rapidly quantified, so that pathological reflux and physiological reflux are diagnosed and distinguished, and the accuracy rate is high.

The invention relates to a chemiluminescence kit for detecting pepsinogen II. The kit comprises an enzyme marker, a luminescent substrate solution and an anti-interference buffer system, the anti-interference buffer system comprises a nonionic surfactant, animal-derived IgG (immunoglobulin G), an antioxidant and catalase; the chemiluminescence kit also comprises a PG II calibration product and a PG II quality control product. The core active component of the enzyme marker is beta-galactosidase. The enzyme marker further comprises an anti-PG II monoclonal antibody marked by beta-galactosidase, bovine serum albumin, trehalose, cane sugar, MgCl2. 6H2O, sodium chloride, Proclin300 and a phosphate buffer solution. According to the kit, a brand-new anti-interference buffer system is introduced on the basis of a spatial proximity chemiluminescence analysis method, so that the resistance to clinically common interferents (such as hemolysis, lipemia, jaundice and heterotropism antibodies) is remarkably improved, and the detection result is more accurate and reliable.

The present invention provides antibodies specifically binding to Pepsinogen C and compositions and kits comprising such antibodies. Furthermore, provided are polynucleotides encoding such antibodies, host cells expressing said antibodies, methods of producing such antibodies and diagnostic methods using such antibodies.

The invention provides a PGI monoclonal antibody combination for pepsinogen I detection and application thereof, the PGI monoclonal antibody combination for pepsinogen I detection comprises a first antibody and a second antibody, the first antibody comprises a first light chain variable region and a first heavy chain variable region, and the second antibody comprises a second light chain variable region and a second heavy chain variable region. The second antibody comprises a second light chain variable region and a second heavy chain variable region; wherein the amino acid sequence of the first light chain variable region comprises VL-CDR1-3 as shown in SEQ ID NO: 1-3; the amino acid sequence of the first heavy chain variable region comprises VH-CDR1-3 as shown in SEQ ID NO: 4-6; the amino acid sequence of the second light chain variable region comprises VL-CDR1-3 as shown in SEQ ID NO: 7-9; and the amino acid sequence of the second heavy chain variable region comprises VH-CDR1-3 as shown in SEQ ID NO: 10-12. The monoclonal antibody combination can improve the sensitivity and accuracy of in-vitro detection.

The utility model discloses a pepsinogen II detection kit which comprises a heat preservation shell, a refrigeration base movably mounted at the bottom of the heat preservation shell through a second thread, and a temperature conduction mechanism movably inserted into the refrigeration base, and the temperature conducting mechanism is used for rapidly transmitting the refrigeration temperature of the refrigeration base into the heat preservation shell. The pepsinogen II detection kit solves the problems that when an existing pepsinogen II detection kit is used, refrigeration needs to be performed by depending on external refrigeration equipment, so that a detection reagent can be stored for a long time, and the storage temperature of the reagent cannot be guaranteed when the pepsinogen II detection kit is used at high frequency.

The present invention provides antibodies that specifically bind to pepsinogen C and compositions and kits comprising such antibodies. Furthermore, polynucleotides encoding such antibodies, host cells expressing the antibodies, methods of producing such antibodies and diagnostic methods using such antibodies are provided.

The invention belongs to the technical field of gene engineering and enzyme engineering, and particularly relates to an engineering strain for efficiently expressing recombinant human pepsinogen rhPGA5 as well as a construction method and application of the engineering strain. The engineering strain is constructed by replacing genes to be knocked out in aspergillus niger with an hPGA5 expression cassette, while the genes to be knocked out are knocked out, the copy number of the hPGA5 gene is increased, and the constructed engineering strain can produce recombinant human pepsinogen rhPGA5 at high yield, can be used for industrial production of human pepsin, and has a good application prospect. The problems that in the prior art, the pepsinogen extraction process is complex, the source is limited, and the heterologous production yield is low are solved.

The present invention provides antibodies specifically binding to Pepsinogen A and compositions and kits comprising such antibodies. Furthermore, provided are polynucleotides encoding such antibodies, host cells expressing said antibodies, methods of producing such antibodies and diagnostic methods using such antibodies.

The invention relates to the technical field of early gastric cancer screening, and discloses a novel biomarker detection method for early gastric cancer screening, which comprises the following steps: S1, preparation before detection: performing joint detection on pepsinogen, gastritin-17, carcino-embryonic antigen and carbohydrate antigen 72-4, and preparing corresponding detection reagents and standard substances; s2, sample collection: adopting a disposable sterile blood taking needle and a vacuum tube, and extracting 3-5ml of venous blood through sterile operation; s3, sample treatment and storage: separating serum through a centrifugal machine, and after the treatment is completed, putting the sample into a storage device for storage; and S4, detection operation: preheating the detector, and then calibrating the detector by a standard product. In the invention, the pepsinogen, the gastritin-17, the carcino-embryonic antigen and the carbohydrate antigen 72-4 are jointly detected, so that the problem of low accuracy possibly caused by the fact that a marker detection method is mostly adopted in the existing early gastric cancer screening is solved.

The present disclosure provides antibodies that specifically bind to pepsinogen A, as well as compositions and kits comprising such antibodies. Furthermore, provided are polynucleotides encoding such antibodies, host cells expressing the antibodies, methods of producing such antibodies, and diagnostic methods using such antibodies.

The present invention relates to the field of medical testing technology, and discloses a pepsinogen I and pepsinogen II joint detection kit and a preparation method thereof, comprising a PVC plate, a sample pad, a quantum pad, a nitrocellulose membrane and blotting paper, the quantum pad is fixed with quantum dot-labeled pepsinogen I antibody and quantum dot-labeled pepsinogen II antibody, the nitrocellulose membrane is fixed with a detection line 1, a detection line 2 and a quality control line composed of goat anti-mouse IgG composed of a secondary antibody for recognizing pepsinogen I antibody and a secondary antibody for pepsinogen II antibody. The present invention can jointly detect pepsinogen I and pepsinogen II in serum with high sensitivity, and can be applied to rapid diagnosis in clinical practice and rapid diagnosis on site, which is not only conducive to promotion in tertiary and large hospitals, but also conducive to use in primary medical units.

The utility model relates to the technical field of kits, in particular to a pepsinogen I determination kit with a quantitative sampling mechanism. According to the technical scheme, the kit comprises a kit body and reagent bottles, a kit cover is installed at the upper end of the kit body, a stabilizing frame is arranged in the kit body, a test tube groove and a reagent groove which are used for storing the reagent bottles and test tubes are formed in the stabilizing frame, the interior of each reagent bottle is divided into quantitative cavities with the same size through a *-shaped partition plate, and sealing stickers are fixed to the upper ends of the quantitative cavities. And a bottle cap is mounted at the upper end of the reagent bottle. The reagent bottle is divided into a plurality of quantitative cavities by utilizing the *-shaped partition plates, during sampling, only a certain quantitative cavity needs to be opened for sampling, the quantitative sampling difficulty is reduced, the reagent bottle and a test tube can be clamped by utilizing dislocation of the movable plates, the stability of the reagent bottle and the test tube is kept, and the safety of storage and transportation is improved.

The invention discloses a sea cucumber peptide applied to auxiliary protection of acute gastric mucosal lesion and a preparation method thereof, and relates to the technical field of biological peptides, and the preparation method comprises the following steps: step 1, taking fresh sea cucumbers, and sequentially carrying out cleaning, acid-base soaking for ash removal and pulping treatment to obtain ground sea cucumber pulp; step 2, boiling the ground sea cucumber slurry at constant temperature and constant pressure, cooling to 55 DEG C, and performing enzymolysis by multiple compound enzymes to obtain enzymatic hydrolysate; step 3, heating the enzymatic hydrolysate to 85 DEG C and carrying out enzyme deactivation treatment for 5 minutes to obtain a protein peptide mixed solution; and 4, separating the protein peptide, and drying to obtain the sea cucumber peptide particles. The sea cucumber peptide applied to auxiliary protection of acute gastric mucosal lesion is obtained, it is detected that interleukin 1 beta (IL-1beta), interleukin 6 (IL-6), pepsinogen 1 (PG1), pepsinogen 2 (PG2) and tumor necrosis factor alpha (TNF-alpha) in rat serum have significant differences from a control group, and the purpose of auxiliary protection of acute gastric mucosal lesion is achieved.

This application relates to the technical field of bioassay, specifically disclosing a combined detection kit for pepsinogen I and pepsinogen II and its application. The disclosed combined detection kit for pepsinogen I and pepsinogen II includes: a sample pad, a conjugate pad, a coating membrane, and an absorbent pad; the coating membrane is an NC membrane coated with antibody; the conjugate pad is prepared by pretreating and drying a fiber membrane, followed by spraying time-resolved fluorescent microspheres labeled with antibody; the pretreating solution is a solution of pH 8.0-8.4 + 8-12mM BS + 0.8-1.2% BSA + 0.8-1.2% PEG4000 + 0.02-0.03% surfactant + 4-6% sucrose. Using the detection kit and method provided in this application for the combined detection of pepsinogen I / pepsinogen II exhibits high sensitivity, accuracy, and repeatability.

The invention discloses a preparation method and application of recombinant pepsin, and belongs to the technical field of enzyme engineering. The invention mainly aims to provide a method for commercially preparing recombinant pepsin and application of the recombinant pepsin, so that animal-derived pepsin is replaced by the recombinant pepsin to solve the current application problem. Specifically, the method comprises the following steps: introducing a pepsinogen coding gene containing a signal peptide as shown in SEQ ID NO.2 into pichia pastoris host bacteria to obtain recombinant pichia pastoris; performing high-density fermentation on the recombinant pichia pastoris to obtain fermentation liquor containing recombinant pepsinogen; performing ultrafiltration concentration and filter washing dialysis on the fermentation liquor to obtain a zymogen solution, and activating the zymogen solution under an acidic condition to obtain an active recombinant pepsin solution; and purifying the recombinant pepsin solution to obtain the recombinant pepsin. The zymogen produced by the method disclosed by the invention is high in yield, and the pepsin obtained from the zymogen has the same function as the extracted pepsin.

The invention discloses a SrTiO3-In2S3 heterojunction photosensitive material and a sensor and application thereof, the photosensitive material adopts a two-step hydrothermal method to successfully construct an II-type heterojunction, and the separation efficiency of photon-generated carriers is remarkably improved, so that the photocurrent response is enhanced; a photosensitive material is modified on the surface of a tin oxyfluoride electrode to form a composite electrode, then the composite electrode and a micro-fluidic chip construct a micro-fluidic photoelectrochemical immunosensor, and a specific antibody is fixed to serve as a biological recognition element. According to the sensor, trace consumption of samples and reagents is realized, and a controllable reaction environment is provided. When the sensor is used for detecting pepsinogen I, the sensor shows a wide linear range, high sensitivity and excellent selectivity and stability, the lower detection limit is as low as 0.1 pg.mL <-1 >, the upper limit can reach 1 mu g.mL <-1 >, and the sensor has a wide application prospect in the field of clinical disease marker detection.

The utility model belongs to the technical field of serum detection equipment, and particularly relates to serum detection equipment for gastric cancer early screening, which comprises a detection box, a treatment groove and a detection groove are formed in the detection box, a treatment mechanism comprises a treatment box and a pick-and-place assembly, and an extractor and a dilution tube are fixed at the output end of the pick-and-place assembly; the detection mechanism comprises a driving motor, a driving screw rod and a lifting plate, a plurality of placement grooves are formed in the top of the lifting plate, and detection plates are detachably connected into the placement grooves; serum is placed in the treatment box and diluted through the dilution pipe, then the taking and placing assembly drives the suction device to extract a sample, the serum is dropped into the detection hole in the detection plate, the lifting plate and the detection plate are driven by the driving motor to move to a detection area, and the detection area is detected. The detection box is used for detecting pepsinogen I (PGI), pepsinogen II (PGII) and the ratio of PGI to PGII in serum through a chemiluminescence method.

The invention belongs to the field of preparation of affinity polymer microspheres, and relates to PS latex particles with high reactivity with a pepsinogen antibody as well as a preparation method and application of the PS latex particles. Crosslinked polystyrene is adopted as an inner core, the structural stability and the refractive index property of the latex particle are guaranteed, the acrylate monomer and the acrylic copolymer are adopted as a shell layer, adjustable carboxyl reaction sites are provided on the surface of the latex particle, and the polyacrylate component with the low glass transition temperature has the rubbery state characteristic, so that the stability of the latex particle is improved, and the service life of the latex particle is prolonged. The carboxyl on the shell layer has higher reaction efficiency. The carboxylated PS latex nanoparticles can be used as a raw material of a kit for latex turbidimetry detection of gastric cancer markers, the reaction efficiency of latex particles and pepsinogens (PGI and PGII) is improved, the detection performance of immunoturbidimetry is enhanced, and the obtained latex kit has increased detection sensitivity and a linear range.

The present invention discloses an anti-pepsinogen II antibody or a reagent and kit for detecting pepsinogen II, relating to the field of antibodies. The anti-pepsinogen II antibody disclosed in the present invention comprises a heavy chain complementary determining region and a light chain complementary determining region. The antibody provides an important raw material source for the detection of pepsinogen II and has improved affinity or activity.

The invention discloses a stomach cancer digital PCR detection kit, and belongs to the technical field of stomach cancer in-vitro detection. The invention prepares the stomach cancer digital PCR detection kit for simultaneously detecting related genes of pepsinogen I (PGI), pepsinogen II (PGII) and gastrin 17 (G-17), the kit simultaneously detects and analyzes the related genes of the stomach cancer through a digital PCR method, the detection result is accurate, the omission ratio of detection is reduced, the sensitivity, specificity and sensitivity are relatively high, and the kit can be used for detecting and analyzing the related genes of the stomach cancer. Meanwhile, the quantitative analysis effect is achieved, data support can be provided in the screening process or the subsequent treatment process, and potential clinical application value is achieved.

The invention provides a PGII monoclonal antibody combination for pepsinogen II detection and application thereof.The PGII monoclonal antibody combination for pepsinogen II detection comprises a first antibody and a second antibody, the first antibody comprises a first light chain variable region and a first heavy chain variable region, and the second antibody comprises a second light chain variable region and a second heavy chain variable region; the second antibody comprises a second light chain variable region and a second heavy chain variable region. The monoclonal antibody combination can improve the sensitivity and accuracy of in-vitro detection.

The invention discloses a kit for diagnosing inhalation pneumonia and a preparation method thereof, and belongs to the technical field of inhalation pneumonia detection. The kit for diagnosing the inhalation pneumonia comprises a PVC (polyvinyl chloride) bottom plate, a sample pad, a gold mark pad, a nanofiber membrane, a liquid absorption pad and a plastic card shell, a detection line 1, a detection line 2 and a quality control line are fixed on the nanofiber membrane; the detection line 1 and the detection line 2 consist of a colloidal gold labeled pepsinogen I antibody secondary antibody, a colloidal gold labeled pepsinogen II antibody secondary antibody; the preparation method comprises the following steps: under a nitrogen atmosphere, firstly, heating a chloroauric acid aqueous solution to be boiled, quickly adding a trisodium citrate aqueous solution, and boiling; cooling to room temperature, slowly adding an SH-PEG-COOH solution, reacting, standing overnight, centrifuging, washing, and adding a confining liquid to obtain a colloidal gold solution; the kit prepared by the invention has better accuracy and specificity and is convenient to operate.

The present invention discloses an anti-pepsinogen II antibody or a reagent and kit for detecting pepsinogen II, relating to the field of antibodies. The anti-pepsinogen II antibody disclosed in the present invention comprises a heavy chain complementary determining region and a light chain complementary determining region. The antibody provides an important raw material source for the detection of pepsinogen II and has good affinity or activity.

The invention provides a homogeneous chemiluminescence determination kit and a quantitative determination method of pepsinogen I. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises a luminescence donor molecule coupled with a first antibody of pepsinogen I and a reaction buffer solution; and the R2 reagent comprises a light-emitting receptor molecule coupled with a pepsinogen I second antibody and a reaction buffer solution. A donor / receptor double-labeled antibody sandwich reaction is adopted, one-step incubation is achieved, solid-phase separation and washing are not needed, the process is remarkably simplified, and the detection time is shortened; by collecting the luminous intensity at 620 nm and 665 nm and quantifying the luminous intensity by a specific value signal, errors caused by sample turbidity, color and volume fluctuation and instrument drift can be effectively corrected; meanwhile, a protein blocking agent, a chelating agent and a heterophilic antibody blocking component are introduced into a reaction buffer system, interference of non-specific adsorption and rheumatoid factors / heterophilic antibodies is reduced, and adaptability to complex matrixes such as bile fluid and serum / plasma is enhanced.

The application provides a PGII monoclonal antibody combination for detecting pepsinogen II and application thereof, the PGII monoclonal antibody combination for detecting pepsinogen II comprises a first antibody and a second antibody, the first antibody comprises a first light chain variable region and a first heavy chain variable region, and the second antibody comprises a second light chain variable region and a second heavy chain variable region. The monoclonal antibody combination can improve the sensitivity and accuracy of in vitro detection.

This invention discloses the application of a traditional Chinese medicine composition in the preparation of drugs for the prevention and treatment of gastritis. The composition comprises the following ingredients: Astragalus membranaceus, ginseng, Atractylodes macrocephala, fermented Cordyceps militaris powder, Lycium barbarum, Hedyotis diffusa, Fagopyrum dibotrys, Bistorta officinalis, Panax notoginseng, and Caesalpinia sappan. This invention is the first to discover and confirm that this traditional Chinese medicine composition can be used to prevent or treat chronic gastritis, especially chronic atrophic gastritis. Experiments have verified that it can significantly prevent or improve the pathological state of the gastric mucosa in rats with chronic atrophic gastritis. Specifically, it can significantly increase the serum pepsinogen I / II ratio, decrease gastrin-17 levels, reverse gastric gland atrophy, and repair the morphological structure of the gastric mucosa, achieving a systematic repair of the function and structure of the CAG gastric mucosa. Furthermore, the ingredients in this traditional Chinese medicine composition are rationally combined, and no obvious toxic side effects were observed under experimental conditions, demonstrating good safety and potential for long-term use.