93 results about "Chyle" patented technology

The invention discloses a detection kit for glycocholic acid for eliminating chyle interference. The detection kit comprises a reagent R1, a reagent R2 and a calibrator. The reagent R1 contains buffer solution a, chyle elimination agent, stabilizer a, surfactant a, reaction promoter a and preservative a; the reagent R2 contains buffer solution b, latex particles of antihuman glycocholic acid monoclonal antibody, stabilizer b, surfactant b, preservative b and reaction promoter b; the calibrator contains buffer solution c, glycocholic acid standard, stabilizer c, surfactant c, reaction promoter c and preservative c. The detection kit is short in detection time, strong in anti-interference capacity, good in stability and repeatability and high in accuracy and detection sensitivity.

The invention relates to the technical field of medical examination, and particularly relates to a latex immunoturbidimetry serum pepsinogen I detection kit for eliminating chyle interference. The kit provided by the invention comprises (1) a pepsinogen I calibrator; (2) a reagent 1 with a preset chyle remover; and (3) a reagent 2 containing the monoclonal antibody and polyclonal antibody coated latex particles against human pepsinogen I. According to the kit provided by the invention, the combination reaction between a substrate to be detected in a sample and a specific antibody in the reagent is amplified through the latex agglutination effect; with the given wavelength, the turbidity formed by the reaction is related to the content of the substrate to be detected, thus the content of the substrate to be detected is calculated. The kit provided by the invention is used for detecting the content of pepsinogen I in human serum, has high sensitivity and good specificity, and can eliminate chyle interference in the sample; moreover, the kit is simple and quick to operate and has high practicability and wide application range.

The invention relates to the technical field of medical examination and particularly relates to a latex immunoturbidimetry type pepsinogen II detection kit capable of eliminating chyle interference. The kit provided by the invention comprises: (1) a pepsinogen II calibrator; (2) a reagent 1 with a preset chyle remover; and (3) a reagent 2 containing a monoclonal antibody of anti-human pepsinogen II and polyclonal antibody coated latex particles. According to the kit using the method, a conjugation reaction of substance to be detected in a sample and specific antibodies in the reagents is amplified by a latex agglutination effect, turbidity formed by the reaction is associated with the content of the substance to be detected under a given wavelength, so that the content of the substance to be detected can be calculated. The kit provided by the invention is used for detecting the content of pepsinogen II in human serum, and the kit is high in sensitivity, good in specificity, capable of eliminating chyle interference in the sample, quick and convenient to operate, strong in practicability and is wide in application range.

The invention discloses a cryopreservation method for a tissue block used for cell culture. The method includes: firstly performing homogenate treatment on a tissue into a chyle state, then adding a refrigerating fluid and mixing them uniformly, subpackaging the mixture into a freezing tube containing sucrose, DMSO and glutathione, then placing the freezing tube in a cryopreservation box and letting the box stay overnight in a -80DEG ultra low temperature refrigerator, and finally putting the freezing tube into liquid nitrogen for long-term preservation. During unfreezing, centrifugal washing by an unfreezing solution and a culture solution is performed in order, then the tissue block can be directly subjected to inoculated culture or can be digested into a single cell and is then subjected to culture. The method provided in the invention firstly employs a homogenizer to treat the tissue into a chyle state, thus facilitating permeation of a cryoprotectant into tissue cells and removal of intracellular water. At the same time, the impermeable protective agent sucrose added in the refrigerating fluid is also conducive to removal the water from the tissue cells. The adding of the antioxidant substance glutathione helps alleviate the damage of oxygen free radicals on intracellular protein. After unfreezing, the tissue block is directly inoculated in a culture bottle, and the adherent survival rate is over 96%.

The invention relates to a kit for measuring the pepsinogen I/II content of human serum. The kit comprises reagents R1 and R2 and a calibration solution, wherein the reagent R1 is a buffer solution of which the PH value is 7.2 to 8.6, the reagent R2 is an anti-human PGI antibody latex reagent or anti-human PGII antibody latex reagent, and a standard substance is recombinant protein containing quantitative PGI or PGII or natural PGI or PGII extracted from the human serum. The kit is characterized by further comprising a reagent R3, wherein the reagent R3 is used for eliminating chyle and is composed of octyl methoxycinnamate, polyoxypropylene polyoxyethylene propylene glycol ether and polyethylene glycol-sulfate chitosan enzyme. The kit has the advantages of high detection sensitivity and strong anti-chyle interference capability and is suitable for detecting severe chyle.

The invention discloses a method for detecting blood chyle degree, determining the chyle degree of blood sample, judging if the blood can be used for clinic according to the result. It uses standard nephelometer card and pipe, the blood chyle degree can be detected through judging the definition of the image on card, the chyle degree is divided into light, middle and heavy chyle, provides gist for clinic blood.

The invention relates to a bio-safety formaldehyde scavenger and a preparation method and application thereof. A moderate fermentation strain is added into a cooked soybean product for fermentation for several days, so that protein of the cooked soybean product is fermented and enzymolysed into soybean peptides and amino acid; then distilled water is added, and after stirring, fermentation is continued under the sealing condition to obtain chyle-shaped product; spray drying is carried out on the product to obtain the bio-safety formaldehyde scavenger. The bio-safety formaldehyde scavenger is adjusted to be alkalescent after being diluted with water, and formaldehyde in the indoor air can be effectively removed through spraying, application or aeration adsorption treatment at the room temperature. The bio-safety formaldehyde scavenger has the advantages that the production cost is low, not only does the bio-safety formaldehyde scavenger have a good effect of removing the formaldehyde, but also the product generated after the formaldehyde is removed has no toxicity and pollution, and the secondary pollution to the environment cannot be caused.

The present invention features compositions and methods for treating gluten-related disorders. We describe compositions comprising one or more metabolites produced by Lactobacillus paracasei CBA L74, International Depository Accession Number LMG P-24778 that reduce cellular entry of gliadin peptides. The compositions may include a physiologically acceptable carrier, for example, a food product or a pharmaceutical carrier. The compositions can be administered to a subject having a gluten-related disorder, for example, celiac disease or gluten sensitivity.

The invention discloses infant milk powder for chyle backflow obstruction. The infant milk powder comprises carbohydrate, protein and lipid; the carbohydrate comprises fructo-oligosaccharide; the lipid is MCT, EPA, DHA, linolenic acid, linoleic acid, oleic acid, arachidonic acid, lecithin, cephalin, taurine, inositol, nucleotide and immune globulin. The infant milk powder is effective in nutrition support therapy for congenital intestinal lymphangiectasia child patients and is capable of effectively improving the clinical symptoms and signs of the child patients and promoting the disease recovery.

The invention provides a procalcitonin (PCT) determination kit. The kit contains a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from the following components: a piperazine-1, 4-diethylsulfonic acid (PIPES) buffer solution, NaCl, MgCl2, chondroitin sulfate, hyaluronic acid, a surfactant and a preservative. And the reagent R2 is prepared from piperazine-1, 4-diethylsulfonic acid (PIPES) buffer solution, goat anti-human PCT antibody coated latex particles, a suspending aid, a stabilizer and a preservative. The invention further provides a preparation method and application of the kit, the kit can effectively avoid interference of chyle particles, latex particles of different specifications, novel surfactants and different protective agents and suspending agents are adoptedat the same time, and the kit is a liquid kit which is high in stability, high in sensitivity, good in repeatability and low in cost.

The invention relates to sample treatment fluid for latex immunoturbidimetry detection, which comprises triglycercide ethoxylate, a nonionic surfactant, glucan (20 thousands) and a buffer. The invention solves the problem that latex immunoturbidimetry can not be adopted for detection because the sample treatment fluid in the traditional reagent has poor effect of eliminating high-degree chyle or turbidity phenomenon of a sample.

The invention discloses a pepsinogen I detection kit, which comprises a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from 50-150 mmol/L of sodium phosphate buffer solution, 2-3 g/Lof bovine serum albumin, methylisothiazolinone with the concentration of 2-3%, 1.0-5.0 g/L of fatty alcohol and ethylene oxide condensate, 3-5 mmol/L of magnesium chloride and 20-30 mmol/L of ammoniumformate, and the balance of deionized water.; and the reagent R2 is prepared from 1.3-2 mg/ml of pepsinogen I antibody emulsion solution, 2-3 g/L of bovine serum albumin, methylisothiazolinone with the concentration of 2-3%, and the balance of deionized water. The prepared pepsinogen I detection kit and the preparation method of the pepsinogen I detection kit have the advantages that the chyle problem can be effectively solved, the anti-interference capability is high, and meanwhile, the stability and the sensitivity are high.

The present invention is directed to one or more macromolecules in a lipid vesicle oral formulation which targets intracellular receptors, in particular for peptides, proteins, nucleic acids and mixtures thereof, optionally in combination with small molecules. The invention encapsulates said macromolecules in a neutral lipid vesicle comprised of one or more cholesteryl esters. Unique properties of macromolecules encapsulated in said vesicles include high oral bioavailability, defined herein as in at least 50%, i.e., often in excess of 50% on the basis of oral to parenteral AUC. Non-limiting examples are provided, for large hydrophilic molecules such as peptides, proteins and nucleic acids which heretofore have been very poorly absorbed by the mammalian intestine. In prior art; said molecules are generally less than 25% bioavailable, even with protective coatings and optionally absorption enhancing component substances in the formulation. An additional feature of the present invention is high tissue concentrations after oral use, a result of rapid uptake of cholestosomes delivered by chylomicrons to body cells. A preferred embodiment is disclosed for insulin, where with cholestosome encapsulation oral bioavailability is at least 66%. Prior to the present invention, oral bioavailability of insulin and other peptides and proteins was maximally 25% and usually between 5% and 10%. Additional preferred examples are provided for one or more macromolecules useful in the treatment of cancer and in particular intracellular targeting in the practice of cancer immunotherapeutics.

A Chinese traditional medicine for curing galacturia caused by filariasis belongs to the Chinese traditional medicine for curing filarisis. The ingredients of the medicine (with weights) are lightyellow sophora root 20g, hawthorn 30g, Tuckahoe 15g, plantain seed 15g, areca 10g, earthworm 10g, dioscorea tokoro 10g and alga 10g; the auxiliary ingredients include cocktree bark 15g, talc 15g, shearer pyrrosia leaf 15g, herb of christina losesetrife 15g and gardenia 15g under the situation of moist heat; fried carrail pollen 10g and amber 10g under the situation of red chyle and difficult excretion with pain; milk vetch root 12g, codonopsis pilosula 12g, cornel 12g, schisandra fruit 12g, fruit of medicine terminalia 12g and gorgon fruit 12g under the situation of weak spleen and kidney. The medicines used in the prescription are all natural herbs and the Chinese traditional medicine is processed with traditional method; the fetching of material is convenient and the prescription and manufacture method are simple; the expense for manufacturing the medicine is low. The composition of the Chinese herbal medicine is simple and the medicines used in the prescription are all natural ones and the fetching and use are convenient; the manufacture method is simple and the effect is excellent; the price is low; in this way, the herbal medicine is especially fit for people living in remote villages far away from counties and towns; the curing expense for patients with the disease of galacturia caused by filariasis is low which solves the problem that the household income is low and the life is poor and the medical conditions is deficient locally.

The invention discloses a camera-based plasma chyle and hemolysis detection device and method. The method comprises steps: blood or plasma image information in a sample test tube stored in a test tubegrid is taken through the camera; based on the image taken by the camera, a plasma chyle degree and hemolysis degree detection result is generated through a signal processing system; corresponding control signals are triggered, and the control signals are then sent to a motor; the motor rotates according to the control signals of the signal processing system, and the amplitude of rotating drivesthe rotating turn number of a corresponding power wheel; the information of a test tube in a test tube grid, the chyle degree and hemolysis degree detection result of the plasma in the test tube and the control signals of the signal processing system are stored by a storage device; and a power supply provides working power for the camera, the storage device, the signal processing system and the motor. The device and the method disclosed in the invention are high in accuracy and practicability and can be widely used in the technical field of medical equipment.

The invention provides a method for extracting a vascular matrix component in adipose tissue and an application thereof. In the embodiment of the present invention, the adipose tissue is obtained by suction and blowing, the chyle adipose tissue is obtained, then an equal volume of an enzymatic hydrolyzate is added, and the adipose tissue is shaken and digested under the temperature condition of 2DEG C to 8 DEG C to obtain SVF. The adipose tissue is processed by a combination of mechanical shearing and low temperature enzymatic hydrolysis, the dry/progenitor cell yield of the obtained SVF canbe improved.

The invention belongs to the technical field of adipose tissue modification, and particularly relates to a preparation method and application of autologous fatty acid protein milk. The method comprises the following steps: taking adipose tissue, removing a strip structure of fat, standing to remove swelling liquid or centrifugally removing moisture, mixing the adipose tissue and an active component to obtain a tissue mixture, grinding the tissue mixture, and then carrying out ultrasonic emulsification to obtain a chylous product, namely the autologous fatty acid protein milk. The active component comprises one or a mixture of more than one of PRP (Platelet Rich Plasma), glutathione or tranexamic acid. The preparation method comprises the following steps of: breaking the cell structure of the adipose tissue without complete cell structure, and releasing active substances in the adipose tissue cells through grinding and emulsifying operation to obtain a mixture of water, fatty acid and protein, namely obtaining the autologous fatty acid protein milk with a relatively complete extracellular matrix structure. Various growth factors in adipose tissue cells are released, so that tissue repair is carried out.

The invention discloses an antimicrobial-free feed for growing pigs. The antimicrobial-free feed comprises 45-65wt% of corn, 15-25wt% of soybean meal, 1-3wt% of chyle fat, 5-15wt% of wheat bran, 0.2-0.3wt% of a mineral matter premix, 0.02-0.03wt% of a vitamin premix, 0.1-0.2wt% of calcium hydrogen phosphate, 0.1-0.5wt% of stone flour, 0.1-0.2wt% of amino acid, 0.1-0.4wt% of salt, 0.03-0.05wt% of Radix Codonopsis, 0.03-0.08wt% of Radix Astragali, 0.03-0.08wt% of Rhizoma Atractylodis Macrocephalae, 0.03-0.08wt% of dry ginger, 0.03-0.1wt% of Poria cocos, 0.01-0.02wt% of tea tree oil and 0.02-0.04wt% of Fructus Rosae Laevigatae. A preparation method of the antimicrobial-free feed concretely comprises the following steps: receiving, cleaning, metering, crushing, burdening, mixing, granulating, carrying out classified screening, adding a liquid, packaging, and storing. The antimicrobial-free feed has the advantages of enhancement of the nonspecific immune functions of weaned piglets, substantial improvement of the immune effects of swine fever vaccines, improvement of the weight gain, and reduction of the feed conversion rate.

The invention discloses a hemoglobin hemolytic agent and the use thereof, the hemoglobin hemolytic agent comprises a) at least one sufficient amount of a nonionic surfactant for dissolving red blood cells, b) at least one sufficient amount of an amphoteric surfactant for dissolving chyle particles, and c) at least one anionic surfactant that binds to hemoglobin. The pH of the hemoglobin hemolyticagent is between 5 and 8. After being mixed with a blood sample, the hemoglobin hemolytic agent can be used for measurement of hemoglobin content. Since the surfactant which reacts with the chyle particles is added, the interference of sample difference is eliminated, the accuracy of the measurement of the hemoglobin content is ensured, a clinical use range is wider, and a false hemoglobin increase can be prevented.