Chemiluminescent quantitative determination kit for pepsinogen II and preparation method of chemiluminescent quantitative determination kit
A quantitative detection technology for pepsinogen, applied in pepsinogen Ⅱ chemiluminescent quantitative detection kit and its preparation field, can solve the problem of no correlation of gastric acid secretion and achieve high sensitivity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0050] Example 1 Preparation of Pepsinogen II Chemiluminescent Quantitative Detection Kit by Using Carbonate Coating Buffer
[0051] 1. Preparation of PBST solid lotion and diluent:
[0052] 1) The formula of PBST solid lotion is
[0053] K H 2 PO 4 4.8g
[0054] Na 2 HPO 4 •12H 2 O 28.8g
[0055] NaCl 155g
[0056] Tween-20 10mL.
[0057] 2) The mixed PBST solid lotion is dried under reduced pressure and low temperature under the condition of air pressure 500Pa, temperature 10°C and humidity 30%. After 1 hour, it is packed in aluminum foil bags. When using, every 5g of PBST solid lotion is dissolved in 500mL deionized Mix in water to prepare PBST solid lotion dilution.
[0058] 2. Preparation of coated microplates:
[0059] 1) Selection of antibody: use mouse monoclonal antibody.
[0060] 2) Coating of microwell plates: Dilute the pepsinogen II monoclonal antibody to a concentration of 3 μg / mL with 0.02 mol / L carbonate buffer solution with a pH value of 9.6 t...
Embodiment 2
[0100] Example 2 Preparation of Pepsinogen II Chemiluminescence Quantitative Detection Kit by Using Phosphate Coating Buffer
[0101] Coating: Dilute the pepsinogen II monoclonal antibody to a concentration of 2 μg / mL with 0.2 mol / L phosphate coating buffer with a pH value of 6.5, prepare a coating solution, and absorb it at 100 μL / well Coated on a microwell plate at 37°C for 4h. Specifically, the preparation method of the phosphate coating buffer with a pH value of 6.5 is: 68.5 mL of 0.2 mol / L NaH 2 PO 4 solution and 31.5mL of 0.2mol / L Na 2 HPO 4 The solution is mixed.
[0102] Rabbit-derived human pepsinogen II monoclonal antibody was selected as the antibody, and the preparation method of other reagents and components was the same as that in Example 1.
Embodiment 3
[0103] Example 3 Preparation of pepsinogen Ⅱ chemiluminescence quantitative detection kit of the present invention by using Tris-HCl coating buffer
[0104] Coating: Dilute the pepsinogen II monoclonal antibody to a concentration of 0.5 μg / mL with 0.05 mol / L Tris-HCl coating buffer with a pH value of 7.4 to prepare a coating solution, and add 100 μL / well It was adsorbed on a microwell plate and coated at 37° C. for 4 hours. Specifically, the preparation method of the Tris-HCl coating buffer with a pH value of 7.4 is as follows: after mixing 50.0 mL of 0.1 mol / L Tris alkali solution and 42.0 mL of 0.1 mol / L HCl solution, add water to volume to 100mL.
[0105] The preparation method of other reagents and components is consistent with the method in Example 1.
[0106] The using method of kit of the present invention
[0107] 1. Preparation before the experiment:
[0108] 1) Take the kit out of the refrigerated environment and place it at room temperature (18°C~25°C) to ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com