The invention relates to a quantitative Golgi apparatus protein GP73 determination kit which comprises a GP73 antibody coating plate, a GP73 standard substance, a concentrated enzyme conjugate, a concentrated enzyme conjugate diluent, concentrated washing liquid, color developing agent liquid A, color developing agent liquid B, termination liquid and a sealing plate film. A quantitative Golgi apparatus protein GP73 determination method comprises the steps of experiment preparation and detection, wherein the detection step includes sample adding, warm bathing, washing, enzyme conjugate adding, warm bathing, washing, color development, determination and data treatment. The quantitative Golgi apparatus protein GP73 determination kit is high in detection sensitivity, low in sample consumption, simple in operation, short in reaction time and good in stability, and the sensitivity and specificity of the quantitative Golgi apparatus protein GP73 determination kit is 39%-64% and 76%-91% respectively.