Quantitative Golgi apparatus protein GP73 determination kit and detecting method thereof

Abstract

The invention relates to a quantitative Golgi apparatus protein GP73 determination kit which comprises a GP73 antibody coating plate, a GP73 standard substance, a concentrated enzyme conjugate, a concentrated enzyme conjugate diluent, concentrated washing liquid, color developing agent liquid A, color developing agent liquid B, termination liquid and a sealing plate film. A quantitative Golgi apparatus protein GP73 determination method comprises the steps of experiment preparation and detection, wherein the detection step includes sample adding, warm bathing, washing, enzyme conjugate adding, warm bathing, washing, color development, determination and data treatment. The quantitative Golgi apparatus protein GP73 determination kit is high in detection sensitivity, low in sample consumption, simple in operation, short in reaction time and good in stability, and the sensitivity and specificity of the quantitative Golgi apparatus protein GP73 determination kit is 39%-64% and 76%-91% respectively.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a kit for quantitative determination of Golgi protein GP73 and a detection method thereof. Background technique [0002] Liver cancer (HCC) is one of the common malignant tumors in my country. Its early symptoms are not obvious. Once discovered, it is often in the middle and late stage and the best time for treatment has been lost. "Early diagnosis and treatment" is the key to the prevention and treatment of liver cancer. At present, the clinical diagnosis of liver cancer is mainly based on imaging and serum marker alpha-fetoprotein (AFP) examination. Neither of these two methods can realize the early diagnosis of liver cancer. Therefore, it is necessary to develop early diagnosis reagents for liver cancer in clinical practice. [0003] Serological testing is easy to operate, cheap, and suitable for large-scale screening, and has attracted more and more attention fro...

AI Technical Summary

Problems solved by technology

[0004] The currently known detection methods of Golgi glycoprotein GP73 still have many defects, such as complicated operation and long time-consuming, which are not suitable for clinical use

At present, the Golgi glycoprotein GP73 detection kits on the Chinese market have shortcomings such as low accuracy, low sensitivity, and poor repeatability.

Images