Carbon dot with continuous golgi apparatus target imaging capability and preparation method thereof

A technology of targeted imaging and Golgi apparatus, applied in chemical instruments and methods, fluorescence/phosphorescence, nanotechnology, etc., can solve the problems of inaccurate positioning, distribution and dispersion of organelles, achieve high blue fluorescence intensity, avoid modification steps, Good photobleaching resistance and biocompatibility

Inactive Publication Date: 2015-10-21
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, carbon dots have been widely used in biological imaging, but their distribution in a single cell is relatively scattered, and it is impossible to precisely locate a certain organelle in a cell

Method used

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  • Carbon dot with continuous golgi apparatus target imaging capability and preparation method thereof
  • Carbon dot with continuous golgi apparatus target imaging capability and preparation method thereof
  • Carbon dot with continuous golgi apparatus target imaging capability and preparation method thereof

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preparation example Construction

[0038] The preparation method of carbon point of the present invention comprises the steps:

[0039] 1), citric acid pyrolysis: according to the mass ratio of citric acid and L-cysteine ​​is the ratio of 1:0.25~1, take citric acid and add round bottom flask, heat at 150~250 ℃ for 0~20min;

[0040] 2), pyrolysis of the mixture: after step 1) is completed, continue to add a proportional amount of L-cysteine ​​to the round bottom flask, and continue heating at 140-250° C. for 3-60 minutes to obtain a solid product;

[0041] 3), acid-base neutralization: add sodium hydroxide or potassium hydroxide solution to the solid product obtained in step 2), until the pH of the whole solution is 6-8;

[0042] 4), purification: filter the neutral solution obtained in step 3) with a filter membrane of 0.22 μm, then dialyze with a dialysis bag with a molecular weight cut-off of 500-1000 Da for 12-48 hours, freeze-dry after dialysis to obtain carbon dots.

Embodiment 1

[0043] The preparation of embodiment 1 carbon point

[0044] The preparation method of carbon dots is as follows:

[0045] 1), citric acid pyrolysis: 2g of citric acid was added in a 100mL round bottom flask, and reacted for 20min at 200°C in an oil bath;

[0046] 2), pyrolysis of the mixture: step 1) After the reaction is completed, add 2g of L-cysteine, and react at 150°C for 50min;

[0047] 3), acid-base neutralization: the reaction product obtained in step 2) is adjusted to pH 6-8 with potassium hydroxide solution;

[0048] 4) Filter the neutralized solution with a 0.22 μm filter head and then put it into a dialysis bag with a molecular weight cut-off of 500-1000 Da for dialysis for 24 hours. After dialysis, freeze-dry to obtain carbon dots.

Embodiment 2

[0049] The preparation of embodiment 2 carbon dots

[0050] The preparation method of carbon dots is as follows:

[0051] 1), citric acid pyrolysis: 2g of citric acid was added in a 100mL round bottom flask, and reacted at 220°C for 20min in an oil bath;

[0052] 2), pyrolysis of the mixture: step 1) after the reaction is completed, add 0.5g L-cysteine, and react at 200°C for 10min;

[0053] 3), acid-base neutralization: the reaction product obtained in step 2) is adjusted to pH 6-8 with sodium hydroxide solution;

[0054] 4) Filter the neutralized solution with a 0.22 μm filter head and then put it into a dialysis bag with a molecular weight cut-off of 500-1000 Da for dialysis for 24 hours. After dialysis, freeze-dry to obtain carbon dots.

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Abstract

The invention discloses a photobleaching-resisting carbon dot with the continuous golgi apparatus target imaging capability and a preparation method thereof. The preparation method comprises the steps that a small carbon dot skeleton is prepared from citric acid through pyrolysis and condensation at high temperature at first, and then the carbon dot skeleton and L-cysteine are in condensation to form a carbon dot. The surface of the carbon dot has a large number of cysteine residues. According to the carbon dot with the high blue-fluorescence intensity and the continuous golgi apparatus target imaging capability, tedious follow-up modification steps needed by conventional methods are avoided. The absolute quantum yield of the blue carbon dot obtained through the preparation method reaches up to 68%. The blue carbon dot has the good photobleaching resistance and biocompatibility, and brings more accurate help to cell process detection, disease diagnosis and the like.

Description

technical field [0001] The invention belongs to the technical field of biological imaging, and in particular relates to a carbon dot which resists photobleaching and has the capability of persistent Golgi target imaging and a preparation method thereof. Background technique [0002] Golgi apparatus (Golgi apparatus, Golgi complex) is one of the organelles of eukaryotic cells. It is not only a site for protein modification, sorting, and hydrolysis processing, but also a transfer station for secreted substances. The fluorescence imaging of Golgi apparatus plays an important role in the study of intracellular physiological activities and the physiological process of diseases related to Golgi apparatus. Since Lipsky et al. in 1985, NBD C 6 After -ceramide is used for fluorescence imaging of Golgi apparatus, ceramide analogs have become common dyes for Golgi apparatus fluorescence targeting. However, this ceramide analog will also appear on the mitochondria and plasma membrane ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65C01B31/02G01N21/64B82Y40/00B82Y20/00
Inventor 王健李荣生
Owner SOUTHWEST UNIV
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