The invention belongs to the technical field of
biochemistry, and discloses a fluorescent probe based on fused ring oxidized
thiophene and application of the fluorescent probe in
cell imaging. The fluorescent probe based on the fused ring oxidized
thiophene is nano-particles coated with the fused ring oxidized
thiophene. The fluorescent probe is applied to
cell imaging, in particular to
lipid droplet and
lysosome imaging. The fluorescent probe can be used as a
fluorescent imaging dye for lysosomes and lipid droplets, a tracer agent of mutual action between the lysosomes and the lipid droplets,and a tracer agent for
lipid droplet formation and
lipid droplet metabolism. The fluorescent probe shows blue-
fluorescence in the lipid droplets, and shows
red fluorescence in the lysosomes after entering cells, the fluorescent
signal intensity and the color change are monitored, so that the high-sensitivity and high-resolution tracing of the movement of the droplets in a short time is realized;and the distribution and the
metabolism conditions of the droplets are traced through long-time monitoring, and it is proved for the first time that the internal components of the droplets can be recovered by the lysosomes. The fluorescent probe is high in sensitivity and simple to operate.