The invention relates to a hydrolysable
tyramine fluorescent dye compound, the compound is composed of a
tyramine derivative group, an
azide derivative group and a fluorescent dye, the
structural formula of the hydrolysable
tyramine fluorescent dye compound is as shown in the
structural formula IV: # imgabs0 #, R1 and R2 are independently selected from any one or a combination of H, F, Cl, Br,-CH3 and-CH2CH3; n is equal to 1-5, m is equal to 1-6, and preferably, m is equal to 1-3; the fluorescent dye R is a
fluorescein compound or a
cyanine compound. According to the method, the fluorescent dye is dissociated and eluted through a mild
chemical reaction after being covalently labeled with the
antigen, the technical problem that traditional multi-target
dyeing cannot meet the
dyeing requirements of more than eight markers of customers is solved, and
dyeing labeling of multiple targets on the same
tissue section is conveniently achieved. Compared with the existing tyramine dye based on a tyramine
signal amplification technology, the hydrolysable tyramine dye disclosed by the invention has the advantages that free radicals generated after the hydrolysable tyramine dye is oxidized are more active, the marking is faster, the sensitivity is higher, and meanwhile, the
signal amplification effect is stronger.