50 results about "Neutral red" patented technology

The invention belongs to the fields of environmental science and engineering, and in particular relates to a method for preparing a bismuth ferrite photocatalyst capable of efficiently degrading organic pollutants in water by utilizing photocatalysis technology. The photocatalyst bismuth ferrite is synthesized by a solvent method; and during synthesis, a molar ratio of bismuth nitrate to ferric nitrate is 12:1, a xenon lamp is taken as a light source, and the bismuth ferrite is fully mixed with neural red solutions with different concentrations to photo-catalyze and degrade the neutral red dye in aqueous solution. The invention provides a high-efficiency method for removing the neutral read dye from the water. The method can remove over 70 percent of the neutral red dye (the concentration is 10 to 30 milligrams / liter) from the water in 2 hours; moreover, the photocatalyst has the advantages of simple preparation process, large particle size and easy recycling; and the method has the advantages of easy operation, low cost, high efficiency, time conservation, and good industrial application prospect.

The invention relates to a method for preparing an immunocompetent soybean peptide by enzymolysis and membrane separation, belonging to the technical field of soybean protein deep processing. The method comprises the following steps of: by taking defatted soybean protein powder as a raw material, firstly, carrying out acid cleaning on the defatted soybean protein powder to obtain acid washed soybean protein; then carrying out enzyme hydrolysis by using an Alcalase protease under certain conditions to obtain coarse soybean peptide liquid; clarifying the prepared coarse soybean peptide liquid by adopting microfiltration; then carrying out enrichment and desalination treatment by adopting ultrafiltration and nanofiltration; and finally, drying to obtain the powdery immunocompetent soybean peptide. The activity of the soybean peptide is determined by adopting a neutral red phagocytosis test and a lymphopoiesis method, which proves that the prepared soybean peptide has high immunocompetence.

The invention discloses a preparation method and application of a magnetic graphene oxide / chitosan adsorbent. The method comprises the steps that graphite powder is oxidized to graphite oxide, and the graphite oxide is subjected to ultrasonic exfoliation to obtain graphene oxide; 1-15 g of ferrous salts, 1-10 g of ferric salts, 0.1-5 g of graphene oxide and 50-500 ml of distilled water are added into a beaker, ultrasonic treatment is conducted in an ultrasonic device for 1 h to 6 h, NH3.H2O is added to regulate the pH to be 7 to 12, and ultrasonic treatment is conducted for 1 h to 6 h; the obtained product is washed to be neutral through a centrifugal separation method and dried in a vacuum drying oven under the condition of 30 DEG C-100 DEG C, and then a magnetic graphene oxide composite material can be obtained; 0.5-2 g of prepared magnetic graphene oxide is weighed and then dissolved in the distilled water, and ultrasonic treatment is conducted for 1 h to 3 h; 20-100 ml of a magnetic graphene oxide solution and 10-100 ml of a CS solution are taken, mixed and then subjected to ultrasonic treatment; 50% of glutaraldehyde is added into the solution, and ultrasonic treatment is conducted continuously; a NaOH solution is prepared, the pH of the solution is regulated to be 8 to 12, and stirring is conducted for 2 h to 3 h; the obtained product is subjected to centrifugal cleaning repeatedly to make the pH of the product to be about 7, and the cleaned product is put into the vacuum drying oven for vacuum drying; the obtained product is the magnetic graphene oxide / chitosan adsorbent. By adopting the material as the adsorbent, under the action of an exterior magnetic field, rapid separation of the adsorbent from adsorbate is achieved, and the effect of removing dye such as neutral red in waste water is good.

The invention discloses a nucleic acid (DNA / RNA) real-time constant-temperature gene amplification detection method. The method is a rapid and efficient novel isothermal amplification detection method taking microplate reader detection as a core. The method particularly comprises the following steps that a to-be-detected sample DNA / RNR template is added into a 15 microliters-25 microliters of LAMP amplification system with certain neutral red concentration and then put into a microplate reader with the temperature ranging from 60 DEG C to 65 DEG C to detect change of absorbance of a reaction system in real time, and therefore the reaction is indirectly monitored. A reaction signal is expressed in the form of a curve, the curve enters a logarithmic phase within 30 min-60 min to express that a detected sample is positive, and the curve extends all the time but not enters the logarithmic phase to express that the detected sample is negative. The nucleic acid (DNA / RNA) real-time constant-temperature gene amplification detection method is simple, rapid and convenient, a great push function is achieved for subsequent basic-level popularization, compared with a traditional PCR detection method, rapid and safe detection of a gene is achieved, and an important significance in the aspects of blocking transmission routes, effectively controlling and tracking infection sources and effectively controlling outbreak and prevalence of superbacteria is achieved.

The invention provides divaricate velvetplant root and rhizome polysaccharide and application thereof in preparing a medicine and functional food for immune adjustment and tumor resistance. According to the invention, the prepared divaricate velvetplant root and rhizome polysaccharide can promote mononuclear macrophage strain to phagocytose neutral red and release nitric oxide, can promote B cell proliferation and T cell proliferation, can also obviously improve immunosuppressive mouse spleen and thymus indexes, can improve the transformation and the proliferation of T cells and B cells, can increase the level of immunosuppressive mouse white blood cells, can obviously restrain the growth of tumor tissues, can protect organisms, can restrain the decline of weight, can gain weight, and can obviously restrain the migration of epidermic cells in veins. In addition, polysaccharide is the main functional component of divaricate velvetplant roots and rhizomes, has various health-care efficacies, is derived from purely natural materials, does not have any side effects, and can be developed into medicines and health-care products for immune adjustment, tumor resistance and angiogenesis resistance, so that the divaricate velvetplant root and rhizome polysaccharide has favorable market application prospects.

The invention provides a reagent which is capable of sensitively indicating the grain fatty acid value change only by means of visual colorimetric analysis and a detection method of the reagent, and the grain aging degree is indicated by the grain fatty acid value change. The detection reagent is prepared by the components, by weight, of 0.01%-0.1% of neutral red, 0.01%-0.1% of Bromothymol blue, 0.005%-0.05% of sodium carbonate, 10%-50% by volume of propylene glycol and the balance of water; the detection method comprises the steps that (1) appropriate components are weighed and dissolved, and the detection reagent is prepared; (2) a to-be-detected grain sample is placed on a piece of flat paper, the detection reagent is aimed at the grain sample and sprayed to the grain sample by one time, and the color change of the grain sample is observed within 1-10 minutes. According to the grain fatty acid value detection reagent, the detection method and application of the detection reagent, the manner of spraying the reagent to the surface of the grain sample is adopted, and the grain fatty acid value and the aging degree are detected by comparing the grain surface color change with a standard color card. Therefore, the method is easier to operate, the detection is more convenient, no other auxiliary equipment is required, and the operation time is saved.

The invention discloses an amphoteric adsorbent prepared from grain stillage, a preparation method for the amphoteric adsorbent and an application of the amphoteric adsorbent and belongs to the fields of brewage waste resource utilization and environmental protection. The amphoteric adsorbent is prepared through taking the grain stillage as a base material, firstly, carrying out pretreatment by using alkali, carrying out a reaction by using an anion modifier, taking an inorganic salt as a converter, and carrying out a reaction by using a cation modifier. The entire process is simple in operation and low in reaction energy consumption, and the raw materials are wide in source and are economical and readily available. The amphoteric adsorbent prepared by the preparation method has good adsorption performance and has the characteristics of low cost, cyclic utilization and no environmental pollution. The amphoteric adsorbent has the adsorption removal rate of 88% to 99.99% to one of cationic dyes such as methylene blue, basic fuchsin, crystal violet, cationic red, malachite green and neutral red and any of anionic dyes such as acid black 1, acid fuchsin, methyl orange, congo red, acid red 18 and acid orange 7 in wastewater, and the adsorption capacity to each of the cationic and anionic dyes is 1mg / g to 500mg / g.

A high-efficiency surface plasma visible-light-induced photocatalyst composite material (Ag@AgCl)-Ni / RGO having magnetic responsibility is disclosed. Firstly, a Ni / RGO composite material is prepared through a solvothermal method and by adopting graphite oxide and nickel chloride hexahydrate as precursors and adopting glycol as a solvent; then AgCl is generated on the surface of the Ni / RGO through a precipitation-deposition method and through adopting AgNO3 as a Ag<+> source and adopting NaCl as a Cl<-> source; and the AgCl surface is partially reduced into Ag through illumination to finally form the (Ag@AgCl)-Ni / RGO composite material. Under illumination by an xenon lamp having a power of 500 W, neutral red, eosine, methylene blue, methyl orange, rhodamine B, and other types of dye can be degraded in 10 min, 20 min, 35 min, 40 min and 40 min respectively through adopting the (Ag@AgCl)-Ni / RGO as a catalyst, and the degradation rates are all higher than 95%. The visible-light-induced photocatalyst material has excellent visible light catalytic degradation effects and is suitable for catalytic degradation of organic pollutants under visible light.

The invention discloses a preparation method of a TiO2 / biomass activated carbon composite material, wherein the preparation method comprises the following steps: putting shaddock peel in ZnCl2, soaking, calcining, then washing with water, pickling and washing with water to obtain biomass activated carbon; mixing distilled water and anhydrous ethanol evenly, and adjusting the pH to 2.5-3.5, to obtain a solution A; adding anhydrous ethanol into a conical flask, adding activated carbon and glacial acetic acid, then adding tetra-n-butyl titanate on a magnetic stirrer, and stirring evenly to obtaina solution B; and adding the solution A into the solution B, stirring for 3-4 h, allowing to stand, to obtain a gel, placing and aging, drying, grinding, then heating up to 185-195 DEG C in a N2 atmosphere, keeping constant temperature for 1.5-2.5 h, then heating up to 450-470 DEG C, carrying out heat preservation calcination for 3-4 h, cooling, grinding, and thus obtaining the product. The composite material with dual properties of adsorption and photocatalytic degradation is prepared by the method. The composite material has good adsorption and degradation properties on neutral red, methylene blue dyes and formaldehyde.

The invention provides a method for preparing mesoporous boron nitride by urea-assisted heat peeling of the boron nitride. The method comprises the following steps: firstly, preparing a boron nitride material; secondly, uniformly mixing urea and the boron nitride according to the mass ratio of 1 to 40 and then calcining at 600 DEG C to prepare a mesoporous boron nitride material. A neutral red uptake test shows that the uptake amount on neutral red of the prepared high-absorbability mesoporous boron nitride is 898.50mg / g and is 44.4 times as much as the neutral red uptake amount of the boron nitride of 20.22mg / g. The method is simple to operate and low in cost and has a good application prospect in the aspect of treating dyestuff wastewater.

The invention discloses preparation of a covalent organic framework material and an application thereof in metal ion recognition and dye adsorption. The preparation method comprises the following steps: adding 2,4,6-tri-(4-bromo-phenyl)-[1,3,5]-triazinide and pyrene-2,7-bis(4,4,5,5-tetramethyl-[1,3,2]dioxaboropentane) into an organic solvent, carrying out ultrasonic treatment, and carrying out cyclic degassing; and adding a palladium catalyst and an alkaline solution, adding the raw materials into a Schleck tube, vacuumizing the tube and introducing nitrogen, performing circular degassing forthree times, purging the tube with argon, sealing the tube, performing stirring reaction, carrying out suction filtration, washing the product, and carrying out vacuum drying to obtain the covalent organic framework material. The synthesis method is simple to operate and high in yield; the synthesized covalent organic framework material is high in sensitivity and good in selectivity and has good application performance. Metal ions can be quickly and sensitively recognized, a good adsorption effect on the neutral red dye is achieved, and a large amount of the neutral red dye can be adsorbed inan aqueous solution within a short time.

To find out an in-vitro bioassay system capable of ensuring qualities of yokukansan to a higher degree, it is intended to provide a method of bioassaying yokukansan characterized by comprising adding a yokukansan-containing test sample to astroglial cells to be cultivated under the condition of thiamine deficiency, and then determining the pharmacological activity value of yokukansan based on the glutamic acid or neutral red intake level in the cultivated astroglial cells.

Methods previously shown to be effective in imparting a prolonged, non-temperature-related, increase in an intrinsic energy of fluids, including water and ethanol, have been applied to heat generated vapors of these fluids, leading to effective activation of the fluids upon their condensation. In a specific embodiment, a Van de Graaf generator was used to apply an electrostatic field to the vapors. The condensed fluids from these electrostatically activated vapors display enhanced internal mobility, more dynamic solubillization patterns using of sprinkled particles of neutral red dye and a progressive, but limited, reduction in measured weight. Fluids condensing from electrostatically-energized vapors can also impart intrinsic energy to other fluids by simple dilution. The described methods are relevant to an understanding of homeopathy.

The invention discloses a method for single cell cloning and isolation and particularly discloses a method for single-cell cloning and isolation of anchorage-dependent growth animal cells. In single-cell cloning and isolation, cells are dyed in short time through a neutral red solution so that small cell blocks are fast dyed and are visible and the accuracy of the operation is improved. In cell cluster picking, a low-concentration pancreatin solution is used so that cells can fall off from the wall of a vessel, cell damage is reduced and cell survival and proliferation are promoted. The method utilizes a manual pipette to suck a small amount of pancreatin and a small cell cluster local digestion method. Compared with the traditional cloning method, the method simplifies processes and is free of a special material-cloning ring. Compared with the limited dilution method, the method is conducive to cell growth and prevents individual growth of many cells in the independent environment. Compared with the method with the microscope, the method is easy to operate and greatly reduces the probability of pollution.

A flue gas condensate cytotoxicity determination method based on a cell electronic sensor is characterized by comprising the following technology steps:1) preparation of experiment reagents; 2) preparation of a flue gas condensate; 3) cell inoculation culture; 4) flue gas condensate contamination; 5) results and analysis. The invention utilizes a cell sensor chip to monitor cell growth conditionsand influences of different condensate concentrations on cell growth after contamination of cells by a cigarette mainstream flue gas condensate on a real time basis, so as to establish a new, real-time, mark-free and high-flux method for cigarette mainstream flue gas cytotoxicity determination. Compared with a neutral red absorption method, the method of the invention omits a neutral red dyeing step and a complicated cleaning step to simplify a test process; meanwhile, quality control on cells is carried out according to a real-time cell growth curve after inoculation to optimize contamination time and contamination concentration, and monitor influences of different condensate concentrations on the cell growth curve after contamination of cells on a real time basis, so as to obtain a moreaccurate and reliable experiment result.

The invention discloses a preparation method and applications of neutral red-modified polyacrylic acid hydrogel mediator functional material. The preparation method comprises the preparation of polyacrylic acid hydrogel and neutral red grafting reaction, and specifically comprises the following steps: A. by taking acrylic acid as a monomer, N,N'-methylenebisacrylamide as a cross-linking agent and potassium persulfate as an initiator, reacting under the condition of 30-80DEG C, to generate polyacrylic acid hydrogel; and B. weighing 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and neutral red to prepare a mixed solution, placing the polyacrylic acid hydrogel prepared in the step A into the mixed solution, reacting for 24h at 10-50DEG C, to obtain crude neutral red-modified polyacrylic acid hydrogel. The polyacrylic acid hydrogel carrier material has large specific surface area, and is beneficial to improvement of non-quinone mediator loading rate, thus realizing high-performance biocatalysis efficiency.

A method for energizing water and other liquids is described. It is based on previous studies showing the use of ultraviolet (UV) light illuminated of alcohol containing neutral red dye as a means of activating the alternative cellular energy (ACE) pathway. Rather than directly activating the patient, the method's ACE pathway, it is used to activate water and liquids for drinking by the patients. Once activated, a liquid can further activate a larger volume of liquid by simply being added to the larger volume of liquid. The methods should find widespread application in human, animal, agricultural and industrial uses of water and other liquids.

The invention relates to a method for fast screening and preparing spice, particularly relates to a method for fast screening and preparing safe spice based on a cell toxicity testing technology and an extraction separation technology and belongs to the field of spice research and development. The method disclosed by the invention comprises the steps of: carrying out in-vitro cell toxicity test on the initial extractum of the spice by a neutral red uptake assay or an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method on the basis of combining the existing in-vitro cell toxicity testing technology with the extraction separation technology; separating the initial extractum of the spice into different extracts by the existing extraction separation technology, carrying out in-vitro cell toxicity test on each extract, and finding out the main source of cell toxicity; and removing the poisonous part and preparing the safe spice by the existing extraction separation technology. The method has the characteristics that the usage of animals can be effectively reduced; manpower and material resources are saved; pain of a living animal is reduced; the test period is shortened; and the detection efficiency is improved, and the development test cost of the spice is reduced.

The invention discloses a method for determining the content of fluorine in a fluorine-containing liquid during the tantalum and niobium hydrometallurgical process, which comprises the steps of preparing a lanthanum nitrate solution, and transferring the lanthanum nitrate solution to a potentiometric titration bottle for calibration; cleaning the pipeline of a potentiometric titrator with pure water, and then moistening the pipeline of the potentiometric titrator with the lanthanum nitrate solution, wherein the titration type is mv titration, the end point parameter is the first end point, theend point jump is middle, and the jump volume is 100.0mv / ml; adding a standard solution of fluorine into a titration cup, adding pure water, placing the solution in the potentiometric titrator for titration, recording the titration volume of lanthanum nitrate, and calculating the titer of lanthanum nitrate according to a formula 1; adding a sample solution into the titration cup, adding ammonia water, pure water and neutral red in sequence, adjusting the sample solution from yellow to red by a hydrochloric acid solution, then placing the solution in the potentiometric titrator for titration,recording the titration volume of lanthanum nitrate, and calculating the content of fluorine in the sample solution according to a formula 2. The method of the invention has the characteristics of high reproducibility, small relative standard deviation in measurement and high accuracy.

The invention relates to a preparation method for a color-changing scale washing agent. The preparation method comprises the following steps: 1) forming mixed solution by hydrochloric acid, ammonia chloride, ethylenediamine tetraacetic acid disodium salt, and benzotriazole, wherein mass concentration of the hydrochloric acid is 7-8%, mass concentration of the ammonia chloride is 1.1-1.6%, mass concentration of the ethylenediamine tetraacetic acid disodium salt is 0.02-0.04%, and mass concentration of the benzotriazole is 0.01-0.02%; 2) mixing 0.1% of ethanol solution of neutral red and 0.1% ofethanol solution of methylene blue according to a volume ratio of 1:1, and forming neutral red-methylene blue mixed solution; and 3) enabling the mixed solution obtained in the step 1) to be mixed with the neutral red-methylene blue mixed solution obtained in the step 2), wherein obtained mixed solution is the color-changing scale washing agent. Compared with the prior art, the washing agent hasthe advantages of small corrosion to equipment, strong cleaning capacity, safety and non-pollution, failure indication through color-changing and the like.

The invention provides a method for detecting trace mercury and cadmium in water through ICP (inductively coupled plasma) extraction. The method comprises steps as follows: S1: a round-bottom centrifugal plastic pipe is prepared, and 10 mL of a sample solution with the pH being 7.0 is added to the round-bottom centrifugal plastic pipe; two drops of a neutral red indicating liquid are dropped, the solution is adjusted to be yellow with a color toner, a complexing agent and the sample solution are added and mixed sufficiently to form a hydrophobic ionic associate, and pH of the mixed solution is adjusted to 5.0 with a buffer solution after the mixture is uniformly shaken and left to stand; S2: 1 mL of an ammonium pyrrolidine dithiocarbamate solution and 1 mL of a Triton X-114 solution with the volume percentage being 20% are continuously added to the sample solution obtained in S2. In the technological procedure, technical features such as color adjustment, centrifugal shaking, temperature control and the like are added, the detection process can be ensured to be more accurate, the accuracy is improved, and the method is easy to operate, facilitates actual treatment, cannot cause heavier pollution and is favorable for popularization and application.

The invention belongs to the field of microorganisms and in particular relates to a deoxycholate hydrogen sulfide lactose agar medium. The medium comprises the following components: 20.1g / L of GN enrichment fluid, 8.0g / L of lactose, 2.8g / L of sodium thiosulfate, 1.5g / L of sodium citrate, 1.5g / L of ammonium ferric citrate, 0.023g / L of neutral red and 16.5g / L of agar, wherein the pH value of the medium is 7.1-7.3; the GN enrichment fluid per liter contains 20.0g of tryptone, 2.0g of glucose, 1.5g of dipotassium hydrogen phosphate, 3g of mannitol, 5.8g of sodium citrate, 0.33g of deoxysodium cholate, 5.0g of sodium chloride and 2.0g of dipotassium hydrogen phosphate. The deoxycholate hydrogen sulfide lactose agar medium has the beneficial effects that the medium is simple in components, convenient in preparation operation and low in cost and is beneficial to popularization and application; the bacteria grow rapidly and the mutation rate is low; the medium is convenient to observe and analyze.

The invention discloses an application of a neutral red premicelle system in the production of a temperature-responsive fluorescence switching device, and belongs to the technical field of temperature-responsive fluorescence switching devices. The neutral red premicelle system is characterized by the application of the neutral red premicelle system in the temperature-responsive fluorescence switching device. The neutral red premicelle system comprises neutral red and a surfactant, and the surfactant is sodium dodecyl sulfate or sodium dodecyl benzene sulfonate. The neutral red premicelle system is relatively sensitive to temperature response, and is reversible, so the neutral red premicelle system has a good application prospect in the production of the temperature-responsive fluorescenceswitching device.

The invention discloses a chromogenic medium used for detecting escherichia coli O157:H7 and other intestinal floras. The medium contains agar, peptone, a yeast extract, sodium chloride, sorbitol, inositol, neutral red, a beta-galactosidase chromogenic substrate, 4-methyl sector ketone-beta-D-glucuronide, isopropyl-beta-D-thiopyran galactoside, bile salt, potassium tellurite and cefixime. The chromogenic medium disclosed by the invention can be used for identifying escherichia coli O157:H7 in coliforms rapidly, simply and conveniently.

The invention relates to the technical field of culture media and in particular relates to a MacConkey improved culture medium with improved separating effect. The improved culture medium is preparedfrom a nitrogen source, cholate, sodium citrate, sodium chloride, lactose, neutral red and agar, wherein a pH is 7.4+ / -0.2. The improved culture medium is capable of remarkably improving the separating effect, and guaranteeing the excellent effect of realizing target bacteria separation in the presence of multiple gram-positive bacteria or more increment of the gram-positive bacteria.