3 results about "Lymphopoiesis" patented technology

This invention relates to the field of biological vaccine technology, specifically disclosing a Mycobacterium tuberculosis Ag85B DNA vaccine, its preparation method, and its application. The HSV2gD signal peptide gene and the Rv1886c gene are linked between HindⅢ / Xbal on the vector pcDNA3.1+ to obtain the pcD-sRv1886c plasmid, which is the Mycobacterium tuberculosis Ag85B DNA vaccine. The pcD-sRv1886c recombinant plasmid of this invention, expressing Ag85B protein, can effectively induce CD4+ secretion of IFN-γ. + T and CD8 + T cells accounted for 2.03% and 1.05%, respectively. Furthermore, the expression of Ag85B protein by this plasmid could stimulate the proliferation of splenic lymphocytes, with a proliferation stimulation index of 5.02. The immune effect generated by the stimulation of Ag85B protein expressed by this plasmid had a significant killing effect on Mycobacterium tuberculosis H37Ra infection.

Upon EBV infection, the inventors found that IL-27 is produced by infected B lymphocytes and IL27RAIL-27 interaction is required for in vitro maintenance and expansion of EBV-transformed B cells, potentially explaining the favorable outcome of the EBV viral disease in IL27RA-deficient patients. In addition, the inventors identified neutralizing anti-IL27 autoantibodies in individuals who developed sporadic infectious mononucleosis, thus possibly phenocopying the IL27RA deficiency. Collectively, these results demonstrate the critical role of IL27-IL27RA axis in immunity to EBV, but also the hijacking of this defense by EBV to promote expansion of infected cells. The IL27-IL27RA could therefore represent a novel therapeutic target to inhibit EBV-driven B lymphoproliferative diseases.

The invention relates to the technical field of medicines and chemistry, and particularly discloses a medical application of a vertramine type isosteroid alkaloid zhebeisine with a 6 / 6 / 5 / 6 / 6 / 6 fused ring system, which is separated from a dry bulb of a liliaceae plant Fritilaria hupehensis Hsiao et K.C.Hsia, in preparation of an immunosuppressive drug, and a preparation method of the vertramine type isosteroid alkaloid zhebeisine with the 6 / 6 / 5 / 6 / 6 fused ring system, wherein the vertramine type isosteroid alkaloid zhebeisine has the advantages that the vertramine type isosteroid alkaloid zhebeisine with the 6 / 6 / 5 / 6 / 6 fused ring system is obtained; more specifically, the invention relates to an application in preparation of drugs having significant inhibitory activity on proliferation of T lymphocytes and B lymphocytes. The veratramine type isosteroid alkaloid disclosed by the invention has the advantages of low toxicity and high efficiency, and can be used for preparing immunosuppressive drugs aiming at organ transplantation and / or autoimmune diseases.