47 results about "Mouse Lymphocyte" patented technology

Preparation of agar active polysaccharide is carried out by: pre-treating, extracting for polysaccharide at optimum temperature, removing glue, dialyzing, and drying to obtain final product. The agar active polysaccharide recovery rate reaches to 13%, and polysaccharide content surpasses 76%. It has better breeding effect for mouse lymphocyte and immune activity.

The invention discloses an Enterobacter cloacae Z0206, which has been stored in 'China microorganism strain preservation management committee common micro organism center' at December 3rd, 2007 and whose storage number is CGMCC NO 2279; also discloses is an Enterobacter cloacae Selenium-rich polysaccharide preparation method and applications of Selenium-rich polysaccharide. The invention has advantages that: the invention has simple production process of extracting the Selenium-rich polysaccharide, high product yield and higher purity, higher Selenium-rich levels; the selenium-enriched polysaccharide prepared by the method can significantly improve the lymphopoiesis capability as well as cytokine level of mice with low immune capacity, and has significant role in the regulation of the immune function of mice with low immune capacity; the selenium-enriched polysaccharide prepared by the method can significantly improve the activity of anti-oxidase, to enhance the function of the antioxidant defense system, has a clear protective effect to the oxidative damage of RAW264.7 cells induced by H2O2, which is an effective antioxidant.

The invention relates to recombinant thymosin Beta 4 two-repeat protein and a preparation method thereof. Human thymosin Beta 4 two-repeat gene expression vector pET-22b(+)-T Beta (2) is constructed through recombinant human thymosin Beta 4 full length cDNA combined with PCR technology, human thymosin Beta 4 that can not be expressed directly in colibacillus is highly actively expressed in colibacillus in the form of two-repeat and purified human thymosin Beta 4 two-repeat protein has biologic activity, can promote multiplication of lymphocyte of mice, has low immunogenicity and lays a foundation for the further research and wide application of human thymosin Beta 4.

The invention discloses a preparation method and application of a recombinant flammulina velutipes immunomodulatory protein that is expressed by Pasteur pichia pastoris, and the preparation method comprises following steps: (1) an encoding FIP-fve gene is cloned; (2) a recombinant Pasteur pichia pastoris expression carrier is constructed; (3) a recombinant Pasteur pichia pastoris transformant is obtained; and (4) the recombinant FIP-fve gene is induction expressed and purified. The preparation method clones an encoding flammulina velutipes immunomodulatory protein, constructs the Pasteur pichia pastoris expression carrier, and consequently obtains the recombinant flammulina velutipes immunomodulatory protein with high yield and high purity. The yield of the recombinant protein that is obtained by the 5-liter fermentation technique reaches 300mg / L to 350mg / L, the purity reaches over 90 percent, and the purified recombinant flammulina velutipes immunomodulatory protein is detected to have the same biologic features and physiological immune activity with natural flammulina velutipes immunomodulatory protein, has procoagulant activity and the promoting functions of mouse lymphocyte proliferation as well as interleukin and interferon secretion, and can induce tumor cell apoptosis.

The invention discloses a probiotic fermented traditional Chinese medicine composition for treating a gastric cancer and a preparation method and application thereof.The fermented traditional Chinese medicine composition is prepared through the steps that extraction and enzymolysis are performed on radix panacis quinquefolii, radix bupleuri, fructus aurantii, radix scutellariae, rhizoma pinelliae, herba salviae chinensis, cuttle bones, bulbus fritillariae thunbergii, radix paeoniae alba, rhizoma chuanxiong, rhizoma cyperi processed with vinegar, rhizoma galangae, radix angelica sinensis, herba cistanche and prepared liquorice roots, fructose, peptone, yeast cream, yeast powder, inorganic salt, a sweetening agent and the like are added, probiotic fermentation is performed, and then dietary fiber and prebiotics are added.The probiotic fermented traditional Chinese medicine composition has the effects of inhibiting gastric cancer cell growth, improving the immunity, regulating the intestinal flora balance, improving the gastrointestinal function and the like and is capable of rapidly achieving the effects and good in mouthfeel and treatment effect.Experiments show that the fermented composition can significantly increase the quantity of mouse T lymphocytes and improve the organism immunity, the inhibition ratio on gastric cancer cells can reach 65%, and therefore the fermented composition is good in treatment effect on the gastric cancer.

The invention discloses a recombinant porcine pseudorabies virus (Herpesviridae) strain used for expression of porcine circovirus type II ORF2 gene. Preservation number of the recombinant porcine pseudorabies virus strain is CCTCC No.V201315. According to the preparation method, PCV2ORF2 gene is inserted into common carrier PG so as to construct transferring plasmid PGO; monolayer ST cells are inoculated with PRVTK<-> / gG<-> / gE<-> virus by 2h of adsorption; ST cells are transfected with plasmid PGO, wherein fusion degree of the monolayer ST cells is 80 to 90%; the recombinant porcine pseudorabies virus PGO strain is obtained by plaque purification, and is used for immunization of mouse. It is shown by results that commercial PCV2 inactivated vaccine and a group immunized by the recombinant porcine pseudorabies virus PGO strain are both capable of inducing specific humoral immune response of mouse, antibody titers of both the commercial PCV2 inactivated vaccine and the group are obviously higher than that of a group immunized by DMEM medium, and difference is significant (p<0.05). It is shown by the results of mouse lymphocyte proliferation test that specific ceullar immune response caused by the group immunized by the recombinant porcine pseudorabies virus PGO strain is more obvious than that caused by the commercial PCV2 inactivated vaccine and the group immunized by DMEM medium, and difference is obvious (p<0.05). In addition, the group immunized by the recombinant porcine pseudorabies virus PGO strain is capable of resisting severe attack by PCV2. Therefore application of the recombinant porcine pseudorabies virus strain in development of a novel PCV2 vaccine is possible.

The invention discloses a new Akkermansia muciniphila 139 strain. The strain is collected in the China General Microbiological Culture Collection Center with a collection number of CGMCC No. 16758, and a collection date is November 21st, 2018. The invention also discloses a purpose of the strain. Through separation and authentication, a new strain, i.e., the Akkermansia muciniphila 139, of Akkermansia muciniphila is obtained, the strain can carry out fermentation in vitro to generate a great quantity of short chain fatty acids, and the intestinal health of a host can be maintained. The fermentation in vitro of supernatant can induce the differentiation of mice lymphocyte regulative T cells, and the immunologic function of the host is enhanced. In addition, the bacteria strain also can alleviate enteritis, especially DSS (dextran sulfate sodium)-induced chronic enteritis, and also has a probiotic function.

The invention relates to a recombined extrasin alpha 1 two-strand body protein and a preparation method thereof. Glycin and serine are connected in series with bimolecular extrasin alpha 1 (T alpha 1), a connection mode is as follows: T alpha 1-Gly-Ser=T alpha 1, and an extrasin alpha 1 two-strand body gene expression vector is constructed by synthesized extrasin alpha 1 two-strand body genes so that extrasin alpha 1 which can not be directly expressed in colon bacillus can be efficiently expressed in the colon bacillus in a two-strand body way. The purified extrasin alpha 1 two-strand body protein has biological activity and can promote lymphocytes of small mice to proliferate, restrain tumor cell strains from proliferating and obviously restrain the tumor growth of tumor-bearing mice, thereby establishing a base for the further research and the extensive application of the extrasin alpha 1.

The invention provides a Hyriopsis cumingii enzymolysis polypeptide, preparation and application thereof. The enzymolysis method comprises the following steps that: fresh Hyriopsis cumingii meat is crushed and pulped, and added with water of which the weight is 5 to 6 times of that of the fresh Hyriopsis cumingii meat; the mixture is boiled and filtered; the precipitate is dried and degreased to obtain albumen powder of the Hyriopsis cumingii meat; the albumen powder of the Hyriopsis cumingii meat is soaked in water of which the weight is 6 to 8 times of that of the albumen powder of the Hyriopsis cumingii meat for 11 to 12 hours and subjected to superfine grinding and even pulping, and the pH value of the mixed solution is adjusted to between 8.0 and 9.0; based on the albumen content in the albumen powder of the Hyriopsis cumingii meat, 0.3 to 0.6 percent of alkali protease is added in the mixed solution for enzymolysis for 4 to 6 hours at a temperature of between 40 and 60 DEG C; the obtained supernatant is condensed and dried to obtain the Hyriopsis cumingii enzymolysis polypeptide; and by testing, the content of the total albumen of the Hyriopsis cumingii enzymolysis polypeptide is more than or equal to 80 percent, wherein the contents of asparagic acid, glutamic acid, leucine, lysine and arginine are more than or equal to 50 percent, and the molecular weight is mainly concentrated in two zones of 6,100Da and 26,200Da. By a test for culturing mouse lymphocytes in vitro, the Hyriopsis cumingii enzymolysis polypeptide can be used for preparing a medicament for promoting proliferation of the T lymphocute and a health care food for improving the immunity.

The invention relates to a whey protein peptide product and application thereof in the preparation of drugs health food or food for enhancing the immunity. The whey protein peptide, which is a mixture of micro-molecular bioactive peptide with a molecular weight concentrated in a range of 180-1000, is generated by carrying out enzymic degradation on whey protein which is used as a raw material. Proved by experiments, the whey protein peptide product remarkably improves the multiplication capability of the ConA induced mouse lymphocyte, the delayed type hypersensitivity (DTH) degree, the antibody cellulation function (IgM-PFC) and the mouse NK (Natural Killer) cell viability, and has the function of enhancing the immunity.

The invention relates to a separation and purification method of mouse intestinal epithelium mucosa lamina propria dendritic cells, which comprises the following specific steps: (1) the acquisition of mixed cells; (2) the acquisition of individual karyocytes: preparing cells acquired after the digestion of collagenase into a suspension, slowly adding into a mouse lymphocyte separation liquid according to a volume ratio of 1:1, and centrifuging at 1800 rpm for 25 minutes; sucking the middle buffy coats, adding into 1-2 milliliters of MACS buffer, and centrifuging at 1200 rpm for 5 minutes; and removing the supernate, and suspending the cells again, wherein the MACS buffer is a PBS (phosphate buffer solution) containing 2mM mol / milliliter EDTA (ethylene diamine tetraacetic acid) and 0.5% FCS (fetal calf serum); and (3) the acquisition of intestinal epithelium mucosa lamina propria dendritic cells. By using the separation and purification method, a large number of high-purity and high-activity intestinal epithelium mucosa lamina propria dendritic cells can be conveniently, economically and efficiently acquired in short time; and the acquired cells keep a good antigen presentation capability under in vitro conditions, thereby providing favorable conditions for the in vitro simulation of in vivo antigen presenting cell induced lymphocyte differentiation.

The present invention discloses a Dendrobium composition and preparation method with enhanced immune function.Dendrobium compositions are composed of Dendrobium, astragalus, jujube, wolfberry, longan meat, Poria, yellow essence, and hybrid maltose, which can exert the overall adjustment advantage of compound traditional Chinese medicine. It can regulate the body's immune function and small toxic side effects.Experiments have confirmed that it can promote the proliferation and transformation of mice lymphocytes and the devouring ability of macrophages, which can improve the activity of serum hemolytin levels, carbon outlines, and NK cells.In human health.

The invention belongs to medicine field, and specifically relates to an application of arctigenin to preparing medicaments for treating systemic lupus erythematosus (SLE). Arctigenin is capable of effectively lymphocyte dysfunction of SLE mise and secretion unbalance of multiple cytokines, has substantial treatment effect on systemic lupus erythematosus, and has the potential of being exploited into the medicaments for treating systemic lupus erythematosus.

The invention relates to the field of protein, in particular to a bioactive polypeptide YFGSGFAAPFFIVRHQLLKK and a preparation method and use thereof, and an amino acid sequence of the bioactive polypeptide YFGSGFAAPFFIVRHQLLKK is Tyr-Phe-Gly-Ser-Gly-Phe-Ala-Ala-Pro-Phe-Phe-Ile-Val-Arg-His-Gln-Leu-Leu-Lys-Lys. An in-vitro immune function regulation experiment proves that the polypeptide YFGSGFAAPFFIVRHQLLKK has a relatively good immune regulation function. The bioactive peptide YFGSGFAAPFFIVRHQLLKK can promote an increase of the induction amount of nitric oxide in macrophages, improves the capability of resisting an external pathogen infection of organisms, reduces a morbidity of the organisms, remarkably promotes lymphocyte proliferation of mice, improves the quality of life, and has veryimportant significance for developing food, health-care products and medicines with immunoregulation functions.

The invention discloses a preparation method of Yupingfeng herb residue polysaccharide synbiotic and application of the synbiotic in immunoregulation and belongs to the field of traditional Chinese medicine residue recycling technology and microecologics. The synbiotic is prepared by using rhizopus oligosporus SH to ferment Yupingfeng herb residue polysaccharide (YPD). The preparation method is characterized by taking YPD as a unique raw material to obtain the Yupingfeng herb residue polysaccharide synbiotic through rhizopus oligosporus SH fermentation and including the links of dissolving of YPD, circulating steam sterilization, cooling, inoculation, fermentation and freeze-drying. By the synbiotic, in-vitro proliferation activity, phagocytic activity and NO releasing capability of rice lymphocytes and macrophagocytes are improved remarkably, and expression of IL-6, TNF-alpha, IFN-gamma, NF-kB, TLR-4 and iNOS in cells can be increased; in an LPS-induced chronic inflammation model, the synbiotic remarkably inhibits in-vitro proliferation activity of lymphocytes and remarkably decreases expression of IL-6, TNF-alpha, IFN-gamma, NF-kB, TLR-4 and iNOS in cells. Consequently, the Yupingfeng herb residue polysaccharide synbiotic has bidirectional immunoregulation effect and can serve as an immunoregulator.

A kind of oxadiazole derivative is characterized by having a following formula, and R is shown as below. The oxadiazole derivative provided by the invention has obvious anti-inflammatory effect on ConA induced lymphocyte proliferation in mice. Therefore, the oxadiazole derivative provided by the invention can be used as a potential anti-inflammatory drug. The invention discloses the preparation method of the compound.

The invention discloses novel chrysin and substituted salicylate composites having a general formula below. In the formula, R1 represents CH3 or CH2CH3, R2 represents H and OCH3, R3 represents H, Cl, Br or CH3, and R4 represents H, F, Cl, Br, OH or CH3. The novel chrysin and substituted salicylate composites of the invention have an obvious inhibiting effect on mouse lymphocyte proliferation caused by ConA. In the invention, compounds with high activity are screened out to be used in tests on mouse lymphocyte proliferation caused by anti-CD3 and anti-CD28 antibodies, and the results of the tests show that the compounds can inhibit the mouse lymphocyte proliferation caused by the anti-CD3 and anti-CD28 antibodies in a dose-dependent manner and have the same inhibiting effect with a cyclosporin A positive medicament. Therefore, the compounds are potential nonsteroidal antiinflammatory drugs. The invention discloses a method for preparing the novel chrysin and substituted salicylate composites.

Preparation of agar active polysaccharide is carried out by: pre-treating, extracting for polysaccharide at optimum temperature, removing glue, dialyzing, and drying to obtain final product. The agar active polysaccharide recovery rate reaches to 13%, and polysaccharide content surpasses 76%. It has better breeding effect for mouse lymphocyte and immune activity.

The invention belongs to the bioengineering field, and discloses a double-functional fusion protein which comprises two function structure areas which are formed by an N end of ITAC, an N-LOOP area and a C end of IP-10. The invention also discloses a carrier and a cell of a double-functional fusion protein gene with a code as well as the application of the double-functional fusion protein. Approved by checking mouse lymphocyte multiplication and killing experiment and tumor histomorphology, the double-functional fusion protein of the invention has the anti-tumor effect, thereby obviously strengthening the anti-tumor cell immunity response level, and synchronously stopping the producing of the tumor blood vessel.

The invention relates to recombinant thymosin Beta 4 two-repeat protein and a preparation method thereof. Human thymosin Beta 4 two-repeat gene expression vector pET-22b(+)-T Beta (2) is constructed through recombinant human thymosin Beta 4 full length cDNA combined with PCR technology, human thymosin Beta 4 that can not be expressed directly in colibacillus is highly actively expressed in colibacillus in the form of two-repeat and purified human thymosin Beta 4 two-repeat protein has biologic activity, can promote multiplication of lymphocyte of mice, has low immunogenicity and lays a foundation for the further research and wide application of human thymosin Beta 4.

The invention relates to a preparation method of a wood frog spawn polysaccharide extract, and an application of the wood frog spawn polysaccharide extract in immunity enhancement. The method is characterized in that the method comprises the following steps: extracting wood frog spawn polysaccharides from wood frog spawn as a raw material through an enzymatic hydrolysis technology, filtering or centrifuging, carrying out alcohol precipitation on the above obtained filtered or centrifuged solution with anhydrous ethanol 3 times the volume of the filtered or centrifuged solution to obtain a precipitate which is the polysaccharide extract, freeze-drying or oven-drying to prepare an extract or dry powder; and the obtained ethanol liquid is recovered below 40DEG C. The wood frog spawn polysaccharides have mouse lymphocyte conversion promotion effects on in vitro mouse spleens to different degrees, a good dose-effect relationship exists, and the polysaccharide extract with the concentration of 0.001[mu]g / mL still has a strong lymphocyte conversion promotion effect. In mouse in vivo experiments, a wood frog spawn polysaccharide sample has an obvious lymphocyte conversion promotion effect. In the field of the influences on cytokines in mouse serum, the influences of the wood frog spawn polysaccharides on the outputs of TNF-alpha and IL-1beta in the serum substantially change. The wood frog spawn polysaccharide extract can be used to prepare immune medicines, and can also be used as a food additive. The extraction method and the extract solvent are not limited to that mentioned in the experiments and the embodiments.

The invention discloses a method for mutagenizing a mice lymphocyte. The method comprises the following steps: firstly, separating lymphocyte; secondly, culturing a mice ES cell; thirdly, fusing the lymphocyte with the ES cell; and fourthly, carrying out separation culture on a mutagenic cell. The lymphocyte is used as an important component cell in an animal body and has the advantages of wide sources, easily-obtained raw materials and the like. According to the method disclosed by the invention, the mice lymphocyte is induced to form a stem cell by means of a cell fusion technology; the formed stem cell has the characteristics of the morphology, gene expression, the in vitro differentiation capacity and the like endowed by the mice ES cell, and thus potential hazards of viruses in stem cell induction are avoided. Therefore, the method lays a foundation for wide application of transforming the mice somatic cell into the stem cell and also provides important reference for research and utilization of stem cells of animals or human beings.

The invention provides a Hyriopsis cumingii enzymolysis polypeptide, preparation and application thereof. The enzymolysis method comprises the following steps that: fresh Hyriopsis cumingii meat is crushed and pulped, and added with water of which the weight is 5 to 6 times of that of the fresh Hyriopsis cumingii meat; the mixture is boiled and filtered; the precipitate is dried and degreased to obtain albumen powder of the Hyriopsis cumingii meat; the albumen powder of the Hyriopsis cumingii meat is soaked in water of which the weight is 6 to 8 times of that of the albumen powder of the Hyriopsis cumingii meat for 11 to 12 hours and subjected to superfine grinding and even pulping, and the pH value of the mixed solution is adjusted to between 8.0 and 9.0; based on the albumen content in the albumen powder of the Hyriopsis cumingii meat, 0.3 to 0.6 percent of alkali protease is added in the mixed solution for enzymolysis for 4 to 6 hours at a temperature of between 40 and 60 DEG C; the obtained supernatant is condensed and dried to obtain the Hyriopsis cumingii enzymolysis polypeptide; and by testing, the content of the total albumen of the Hyriopsis cumingii enzymolysis polypeptide is more than or equal to 80 percent, wherein the contents of asparagic acid, glutamic acid, leucine, lysine and arginine are more than or equal to 50 percent, and the molecular weight is mainly concentrated in two zones of 6,100Da and 26,200Da. By a test for culturing mouse lymphocytes in vitro, the Hyriopsis cumingii enzymolysis polypeptide can be used for preparing a medicament for promoting proliferation of the T lymphocute and a health care food for improving the immunity.

The invention discloses novel chrysin and substituted salicylate composites having a general formula below. In the formula, R1 represents CH3 or CH2CH3, R2 represents H and OCH3, R3 represents H, Cl, Br or CH3, and R4 represents H, F, Cl, Br, OH or CH3. The novel chrysin and substituted salicylate composites of the invention have an obvious inhibiting effect on mouse lymphocyte proliferation caused by ConA. In the invention, compounds with high activity are screened out to be used in tests on mouse lymphocyte proliferation caused by anti-CD3 and anti-CD28 antibodies, and the results of the tests show that the compounds can inhibit the mouse lymphocyte proliferation caused by the anti-CD3 and anti-CD28 antibodies in a dose-dependent manner and have the same inhibiting effect with a cyclosporin A positive medicament. Therefore, the compounds are potential nonsteroidal antiinflammatory drugs. The invention discloses a method for preparing the novel chrysin and substituted salicylate composites.

The invention belongs to medicine field, and specifically relates to an application of arctigenin to preparing medicaments for treating systemic lupus erythematosus (SLE). Arctigenin is capable of effectively lymphocyte dysfunction of SLE mise and secretion unbalance of multiple cytokines, has substantial treatment effect on systemic lupus erythematosus, and has the potential of being exploited into the medicaments for treating systemic lupus erythematosus.

The invention relates to a recombined extrasin alpha 1 two-strand body protein and a preparation method thereof. Glycin and serine are connected in series with bimolecular extrasin alpha 1 (T alpha 1), a connection mode is as follows: T alpha 1-Gly-Ser=T alpha 1, and an extrasin alpha 1 two-strand body gene expression vector is constructed by synthesized extrasin alpha 1 two-strand body genes so that extrasin alpha 1 which can not be directly expressed in colon bacillus can be efficiently expressed in the colon bacillus in a two-strand body way. The purified extrasin alpha 1 two-strand body protein has biological activity and can promote lymphocytes of small mice to proliferate, restrain tumor cell strains from proliferating and obviously restrain the tumor growth of tumor-bearing mice, thereby establishing a base for the further research and the extensive application of the extrasin alpha 1.

The invention discloses anti-rat CD3 recombinant immunotoxin as well as a preparation method and application thereof and belongs to the technical field of biology. A genetic engineering technology anda genetic engineering strategy are adopted, and a combination manner of a single-chain antibody and DT390 is changed to improve the affinity; codon series are optimized to obtain three types of recombinant immunotoxin which take a DT structure as a toxic center, and a prokaryotic expression system is constructed; anti-rat single-chain antibody coupled immunotoxin of a targeting T cell receptor CD3epsilon is obtained and T lymphocytes of a peripheral immune organ are eliminated; a transplanting immune model is established by utilizing the immunotoxin, and treatment researches including immunetolerance, autoimmune diseases and the like are carried out. The anti-rat CD3 recombinant immunotoxin proves the effectiveness of eliminating rat peripheral blood and lymph node T cells by the targeting anti-rat CD3epsilon recombinant immunotoxin. The anti-rat CD3 recombinant immunotoxin has application meanings in the aspects of researches of eliminating the rat T lymphocytes, treating the autoimmune diseases, transplanting the organs, treating immune tolerance and treating tumors, and possibly has a potential of pre-clinical pharmaceutical researches and conversion subsequently.

The invention relates to use of oviductus ranae in preparing medicament for curing and preventing diseases caused by immunologic dysfunction. The flow cytometry and the ELISA method proves that the oviductus ranae has a function for immunity adjustment by the results of mouse spleen T-lymphocyte subsets CD3, CD4, CD5, CD8 and the ratio of CD4 / CD8, namely, when the immunity is high, the oviductus ranae has a function for weakening the immunity, and when the immunity is low, the oviductus ranae has a function for enhancing the immunity. The new use of the oviductus ranae proves that the oviductus ranae has a therapeutical effect for the diseases caused by the immunologic dysfunction such as asthma, autoimmune disease, hepatitis and tumor, thereby developing the use of the oviductus ranae inpreparing medicament for curing and preventing the diseases caused by the immunologic dysfunction.

The invention relates to an application of a decoy receptor 3 in preparation of medicine for treating sepsis, which is characterized in that expression of DcR3 in serum of sepsis patients is increased, and DcR3 is relative to mortality of sepsis, DcR3 is participate to generation and development of sepsis, and can reduce the mortality of sepsis mice, reduce inflammation reaction of sepsis mice, inhibit lymphocytic apoptosis of sepsis mice, protect organs such as liver and lung; so that DcR3 has certain treatment effect for sepsis.