Separation and purification method of mouse intestinal epithelium mucosa lamina propria dendritic cells

A technology of dendritic cells and intestinal epithelium, applied in animal cells, vertebrate cells, blood/immune system cells, etc., can solve the problems of LPDC extraction difficulties, achieve good antigen presentation ability, strong operability, The effect of the simple experimental process
CN102382802AInactive Publication Date: 2012-03-21梁廷波
0 Cites 5 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
梁廷波
Publication Date
2012-03-21
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to a separation and purification method of mouse intestinal epithelium mucosa lamina propria dendritic cells, which comprises the following specific steps: (1) the acquisition of mixed cells; (2) the acquisition of individual karyocytes: preparing cells acquired after the digestion of collagenase into a suspension, slowly adding into a mouse lymphocyte separation liquid according to a volume ratio of 1:1, and centrifuging at 1800 rpm for 25 minutes; sucking the middle buffy coats, adding into 1-2 milliliters of MACS buffer, and centrifuging at 1200 rpm for 5 minutes; and removing the supernate, and suspending the cells again, wherein the MACS buffer is a PBS (phosphate buffer solution) containing 2mM mol / milliliter EDTA (ethylene diamine tetraacetic acid) and 0.5% FCS (fetal calf serum); and (3) the acquisition of intestinal epithelium mucosa lamina propria dendritic cells. By using the separation and purification method, a large number of high-purity and high-activity intestinal epithelium mucosa lamina propria dendritic cells can be conveniently, economically and efficiently acquired in short time; and the acquired cells keep a good antigen presentation capability under in vitro conditions, thereby providing favorable conditions for the in vitro simulation of in vivo antigen presenting cell induced lymphocyte differentiation.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of separation and purification of cells, and mainly relates to a method for separation and purification of dendritic cells in the lamina propria of mouse intestinal epithelium. Background technique

[0002] As early as 1973, American scholar Steinman discovered dendritic cells (Dendritic cells, DC) with powerful antigen presentation function. This type of cell is the professional antigen-presenting cell with the strongest function in the body known so far. It can efficiently take up, process and present antigen. Immature DC has a strong ability to migrate, and mature DC can effectively activate naive T cells. Cells are at the center of initiating, regulating, and maintaining immune responses.

[0003] They are usually distributed in small amounts on exposed skin sites, mainly the skin and lining of the nasal cavity, lungs, stomach and intestines. The gut is the largest immune organ in the human body. On the one han...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More