30results about How to "Shorten separation and purification time" patented technology

The invention discloses a method for separating and purifying paclitaxel from taxus chinensis branches and leaves or bark. The method comprises the following steps: firstly, immersing taxus chinensis branches and leaves or bark by methanol, carrying out ultrasonic extraction or reflux extraction, and concentrating at reduced pressure to obtain an extract; secondly, and extracting the extract sequentially by petroleum ether and ethyl acetate to obtain an ethyl acetate extract; thirdly, carrying out chromatography on the ethyl acetate extract through an alkaline aluminum oxide column to obtain a paclitaxel-enriched component, and treating the paclitaxel-enriched component by utilizing a partial precipitation method which uses methanol-water as a system to obtain a crude paclitaxel product; and finally, refining to obtain paclitaxel with purity over 98.5 percent by using flash chromatography as a separation means and methanol-water as an eluting system. The method is simple and convenient to operate, has the advantages of low cost, good repeatability, high efficiency and high product purity, and can be used for large-scale production of paclitaxel.

The invention discloses a method for removing proteins and pigments in ganoderma lucidum crude polysaccharide, which comprises the following steps of: preparing the ganoderma lucidum crude polysaccharide into 10-30mg/m1 aqueous solution; centrifuging the aqueous solution to take a liquid supernatant; sampling the liquid supernatant; adding the liquid supernatant into a radial flow chromatographic column filled with an anion exchanger; then eluting the chromatographic column with pure water to control a sampling flow velocity to 2-10ml/min and an elution flow velocity to 10-50ml/min; collecting an eluent until no saccharide is detected in the eluent; combining the collected eluent; and carrying out post-treatment to obtain a pure product of the ganoderma lucidum polysaccharide. The method has the advantages that: the proteins and the pigments in the ganoderma lucidum crude polysaccharide are removed simultaneously so as to greatly shorten separation and purification time of the polysaccharide, be favorable for reserving the polysaccharide activity maximally and improve the purification efficiency of the ganoderma lucidum crude polysaccharide; organic solvents are not used in an operation process, conditions are mild, subsequent treatment is convenient, and environmental pollution is lightened; and the iron exchange resins have low cost and can be recycled so as to effectively reduce the production cost.

The invention relates to a process for extracting artemisinin from artemisia apiacea. The process uses 200# gasoline as an organic solvent, and extracts by a reflux extracting method and a pressurizing cool circulating extracting method, wherein the process comprises steps like crushing, organic solvent extraction, recycling solvent, hot melting, decoloration, concentration, separating crystals and refining. In comparison with the traditional process, the process provided by the invention has advantages of lower production cost, a simpler process, a higher yield and is more suitable for modern mass production. The process shortens the separation purification time, and raises the purity, quality and stability of the product by cooperating an ion exchange resin and a macroporous absorption resin.

The invention provides a method for preparing total saponins of rhizoma cimicifugae through separation and purification by virtue of a micro emulsion-phase interface extraction technique. The method can achieve the purpose of realizing industrial mass production by virtue of the micro emulsion extraction technique, and the method can save time and reduce the use of an organic solvent; and in addition, the clinical efficacy of the total saponins of rhizoma cimicifugae prepared by the method is better than that of total saponins of rhizoma cimicifugae obtained by a conventional separation and purification method.

The invention discloses a method for separating a reference substance of ergosterol and stellasterol from a traditional Chinese medicine polyporus umbellatus. According to the method, high-purity monomeric compounds of the ergosterol and the stellasterol are prepared from polyporaceae polyporus polyporus(pers.) dry sclerotoids, serving as raw materials, by means of ethanol liquid extraction, petroleum ether decontamination, dichloromethane extraction, sephadex LH-20 column chromatography, re-crystallization and half-preparation. The method is simple and reliable, the raw materials are easy to obtain, the economic cost is low, the specificity is strong, and the method is applicable to large-scale preparation of the reference substance of the ergosterol and the stellasterol; moreover, during extraction and crystallization, ethyl alcohol or methyl alcohol is adopted to reduce the environmental pollution, and the solvent cost is low; by means of the crystallization or preparation method, the separation and purification time of a sample is shortened, the costs of manpower and material resources are reduced, and the economic effect is improved; a reference substance for content determination can be provided for quality control over crude drugs of the traditional Chinese medicine polyporus umbellatus, thus contributing to establishment of a quality control method for the crude drugs polyporus umbellatus.

The invention provides a process method for separating and purifying maize pollen from maize anther. Maize male spikes (the male spikes are extracted and are close to dusting period) are extracted cross the ridge, and anther on the male spikes is rubbed down. The maize anther comprises two parts: pollen capsule walls and pollen wrapped thereby. The pollen and the pollen capsule walls are initially separated by water-soaking and filtering methods, the tissues of the filter residues (the pollen capsule wall) are titrated, and the titrated pollen capsule walls are further soaked in water and filtered so as to further separate the pollen from the pollen capsule walls. The filtrates of filtration twice are mixed together to obtain relatively pure pollen, and the filtrate is filtered, so that the effect of eliminating fine impurities and purifying the maize pollen can be achieved, and the filtrate is lyophilized to obtain pure maize pollen. The method is simple, feasible and high in efficiency, the nutrients of the maize pollen are not destroyed and the maize pollen is not damaged, and the method is suitable for scaled production.

The invention discloses an extraction, separation and purification method of aconitum alkaloid, which belongs to the technical field of alkaloid extraction. The method comprises the following steps of by taking plant tubers containing aconitum alkaloid as raw materials, crushing, sieving, performing ultrasonic-assisted extraction by adopting an acidified ethanol solution, and removing a solvent through deesterification, alkalization and reduced pressure distillation to obtain crude alkaloid, then separating by adopting high-speed counter-current chromatography, finally preparing high-content aconitum alkaloid monomers by adopting reversed-phase high-performance liquid-phase semi-preparative chromatography, carrying out ultraviolet online detection, collecting a target object, and carrying out freeze drying to obtain aconitine, aconitine, neoaconitine and aconitine yunnanense with purity greater than 98.0%. By adopting the preparation method disclosed by the invention, the separated product is high in yield, high in purity, short in process, low in cost and easy to industrialize.