The invention relates to a method for separating and purifying an FMD inactivated
virus antigen by applying a simulated flow
bed, wherein the method comprises the following steps: balancing a
chromatographic column of the simulated flow
bed with a balanced
buffer solution; loading an FMD inactivated
virus antigen sample into the
chromatographic column of the simulated flow
bed, during sample loading, simultaneously carrying out
antigen sample
impurity cleaning and sample
desorption elution as well as chromatographic filler cleaning and regeneration and balanced liquid balancing on the
chromatographic column, and continuously desorbing and eluting the collected and purified sample in the process. The simulated flow bed is applied to separation and purification of the FMD inactivated
virus antigen for the first time, the continuous saturated sample loading separation operation can be realized and the sample loading amount is greatly improved; moreover, through the continuous separation and purification via multiple
monomer columns, the degree of continuity is high, the separation and purification time is greatly shortened, the preparation efficiency is improved and the operation costis reduced. Moreover, the rigid filler is selected specifically, the loading capacity of the filler namely the
pressure resistance is greatly improved, the use amount of the chromatographic filler isreduced, and the service life of the chromatographic column in the simulated flow bed is prolonged.