Separation and purification method for maize pollen
A technology for separation and purification of corn pollen, applied in pharmaceutical formulations, cosmetic preparations, food preparation, etc., can solve the problems of low corn pollen acquisition rate, waste of pollen, low efficiency, etc., shorten the separation and purification time, increase economic benefits, The effect of reducing waste
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[0018] A method for separating and purifying corn pollen, the method comprising the following steps:
[0019] (1) Removing anthers: Squeeze off the anthers on the tassels and put them together for processing;
[0020] (2) Soaking: Soak the anthers in water, the ratio of material to liquid is 1:4 (g:ml), stir well for 1 minute and then let it stand for 1 minute, so that the pollen grains sink and the anther sac wall and some impurities float up;
[0021] (3) Filtration: Filtrate with a 100-mesh filter cloth to obtain the anther sac wall and filtrate A (the mixture of pollen and water obtained from the initial filtration);
[0022] (4) Tissue mashing: the anther sac wall is subjected to tissue mashing to destroy its tissue structure, and then soaked in water with a material-liquid ratio of 1:4 (g:ml), fully stirred for 1 min and then left to stand for 1 min to make the anther sac The remaining pollen grains in the wall sink, and the anther sac wall and some impurities float up;...
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