Rapid separation and purification method of cordyceps militaris fruit body water-soluble peptide polysaccharide
A peptidoglycan, water-soluble technology, applied in the field of extraction of biologically active substances, can solve the problems of cumbersome process, low benefit, pollution, etc., and achieve the effect of process simplification, cost saving and simple process
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Embodiment 1
[0026] Follow these steps:
[0027] 1) drying the fruiting body of Cordyceps militaris, pulverizing, passing through a 200-mesh sieve, and setting aside;
[0028]2) Ultrapure water extraction: According to the ratio of Cordyceps militaris fruiting body powder: ultrapure water = 1:20, extract in three times, the specific ratio of adding water each time is 1:10, 1:5, 1:5 respectively, and the temperature is controlled at Keep at about 100°C for 4 hours, centrifuge at 5,000 rpm for 20 minutes, and collect the supernatant; combine the supernatants extracted three times, and filter through a 0.22 μm membrane to obtain the filtrate component CF1;
[0029] 3) Molecular sieve separation: Put the filtrate CF1 on the column of Superose 6 as the chromatographic gel, the chromatographic instrument is AKTAPurifier 100 equipped with Shimadzu RID-10A differential detector, the mobile phase is ultrapure water, and the flow rate is 1.0ml / min. Three bands 280, 260, 215λm ultraviolet detection ...
Embodiment 2
[0033] In this example, the fruiting body powder of Cordyceps militaris: ultrapure water is 1:30, add water and extract three times at 1:10, 1:10, and 1:10, and the temperature is controlled at about 90°C; the molecular sieve is Sephadex G-50 column The ion exchange column is outside the DEAE Sepharose CL-6B column, and all the other processes, conditions, steps, and materials are the same as in Example 1.
Embodiment 3
[0035] In this example, the fruiting body powder of Cordyceps militaris: ultrapure water is 1:40, add water and extract three times at 1:15, 1:15, and 1:10, and the temperature is controlled at about 80°C; the molecular sieve is Sephadex G-100 column The ion-exchange column is outside the column of DEAE cellulose D-32, and all the other processes, conditions, steps, and materials are the same as in Example 1.
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