12 results about "DEAE-Cellulose" patented technology

This invention discloses the application of Uncaria rhynchophylla neutral polysaccharide URP-W in the preparation of anti-glioma drugs, belonging to the field of anti-tumor technology of medicinal plant polysaccharides. The invention obtains a crude polysaccharide extract from Uncaria rhynchophylla through hot water extraction and ethanol precipitation, followed by purification using a DEAE cellulose column and G200 dextran gel to obtain Uncaria rhynchophylla neutral polysaccharide URP-W. The monosaccharide composition of URP-W includes arabinose, rhamnose, galactose, glucose, xylose, mannose, galacturonic acid, and mannuronic acid in a molar ratio of 17.99:6.32:21.71:27.17:2.56:7.57:16.20:0.49. Experiments have shown that URP-W can inhibit the proliferation of gliomas and induce apoptosis, exhibiting significant anti-tumor activity against glioma cells, providing a new drug raw material and treatment method for the clinical treatment of gliomas.

The invention discloses application of uncaria acidic polysaccharide URP-1 in preparation of anti-prostatic cancer drugs, and belongs to the technical field of antitumor of medicinal plant polysaccharides. The preparation method comprises the following steps: obtaining uncaria total polysaccharide from uncaria through boiling water extraction and ethanol precipitation methods, and then purifying the total polysaccharide through DEAE cellulose and sephadex G200 to obtain the uncaria acidic polysaccharide URP-1. The URP-1 comprises the following components in mole percent: 28.68% of arabinose, 11.08% of rhamnose, 29.55% of galactose, 19.00% of glucose, 4.47% of mannose and 7.22% of galacturonic acid. Experiments discover that the uncaria acidic polysaccharide URP-1 has remarkable anti-tumor activity on DU 145 cells, and a novel medicine raw material and a novel treatment method are provided for clinical treatment of prostatic cancer.

The application discloses a chaga acidic polysaccharide IOP-2 with anti-thyroid cancer activity and application thereof, and belongs to the field of biological medicines. The application adopts a method for extracting chaga polysaccharide by boiling water and precipitating chaga polysaccharide by ethanol, and combines DEAE cellulose column purification technology, and successfully realizes separation of chaga polysaccharide by using the principle that macromolecular polysaccharide is insoluble in organic solvents, and obtains chaga acidic polysaccharide IOP-2 with anti-thyroid cancer activity. The monosaccharide composition of the chaga acidic polysaccharide IOP-2 includes fucose 0.71%, rhamnose 3.33%, arabinose 2.18%, galactose 2.92%, glucose 80.59%, xylan 3.46% and mannose 6.81% in terms of molar percentage. The significant effect of the chaga acidic polysaccharide IOP-2 on anti-thyroid cancer is verified through in-vivo and in-vitro experiments, and a new treatment method is provided for clinical treatment of thyroid cancer.

The invention discloses inonotus obliquus acidic polysaccharide IOP-2 with thyroid cancer resisting activity and application of the inonotus obliquus acidic polysaccharide IOP-2, and belongs to the field of biological medicine. According to the invention, a method for extracting inonotus obliquus polysaccharide by boiling water and precipitating the inonotus obliquus polysaccharide by ethanol is adopted, a DEAE cellulose column purification technology is combined, and the principle that macromolecular polysaccharide is insoluble in an organic solvent is utilized, so that the separation of the inonotus obliquus polysaccharide is successfully realized, and the inonotus obliquus acidic polysaccharide IOP-2 with thyroid cancer resisting activity is obtained; the monosaccharide of the inonotus obliquus acidic polysaccharide IOP-2 is prepared from the following components in percentage by mole: 0.71 percent of fucose, 3.33 percent of rhamnose, 2.18 percent of arabinose, 2.92 percent of galactose, 80.59 percent of glucose, 3.46 percent of xylan and 6.81 percent of mannose. In-vivo and in-vitro experiments prove that the traditional Chinese medicine composition has a remarkable thyroid cancer resisting effect, and a novel treatment method is provided for clinical treatment of thyroid cancer.

The invention discloses application of uncaria neutral polysaccharide URP-W in preparation of anti-glioma drugs, and belongs to the technical field of antitumor of medicinal plant polysaccharide. The preparation method comprises the following steps: obtaining a crude polysaccharide extract from uncaria through a hot water extraction and ethanol precipitation method, and purifying the crude polysaccharide extract through a DEAE cellulose column and sephadex G200 to obtain the uncaria neutral polysaccharide URP-W; the monosaccharide of the URP-W comprises arabinose, rhamnose, galactose, glucose, xylose, mannose, galacturonic acid and mannuronic acid, and the molar ratio of the arabinose to the rhamnose to the galactose to the glucose to the xylose to the mannose to the galacturonic acid to the mannuronic acid is 17.99: 6.32: 21.71: 27.17: 2.56: 7.57: 16.20: 0.49. Experiments find that the URP-W can inhibit proliferation of glioma and cause apoptosis, has remarkable anti-tumor activity on glioma cells, and provides a new medicine raw material and a new treatment method for clinical treatment of glioma.

The application discloses application of Uncariae Ramulus Cruda acidic polysaccharide URP-1 in preparation of anti-prostate cancer drugs and belongs to the technical field of medicinal plant polysaccharide anti-tumor. The total Uncariae Ramulus Cruda polysaccharide is obtained from Uncariae Ramulus Cruda through a method of boiling water extraction and ethanol precipitation, then the total polysaccharide is purified through DEAE cellulose and dextran gel G200 to obtain Uncariae Ramculus Cruda acidic polysaccharide URP-1. The components of the URP-1 include arabinose, rhamnose, galactose, glucose, mannose and galacturonic acid, and the molar percentages are 28.68%, 11.08%, 29.55%, 19.00%, 4.47% and 7.22% respectively. It is found through experiments that the Uncariae Ramulus Cruda acidic polysaccharide URP-1 has significant anti-tumor activity on DU 145 cells, and provides a new drug raw material and treatment method for clinical treatment of prostate cancer.

The invention belongs to the technical field of biological medicine, and particularly discloses a preparation method and application of laxative rhizoma anemarrhenae homogeneous polysaccharide, the preparation method comprises the following steps: taking rhizoma anemarrhenae total polysaccharide as an initial raw material, performing primary separation on the supernate of the solution through an anion-cation exchange resin column formed by connecting FPA90Cl and IR84 in series, and performing secondary separation on the supernate to obtain the laxative rhizoma anemarrhenae homogeneous polysaccharide. Eluting with distilled water to obtain target crude polysaccharide; further performing gradient elution on supernate of the crude polysaccharide solution through a DEAE Cellulose 52 ion exchange chromatographic column, collecting a main elution peak during elution of 0.6 mol / L NaCl, and performing concentration, distilled water dialysis and freeze drying to obtain high-purity target polysaccharide. According to the invention, the influence of the rhizoma anemarrhenae homogeneous polysaccharide solutions with different concentrations on cell proliferation is evaluated, and the dose-dependent effect is proved; the obvious influence of the polysaccharide on the AQP3 and AQP8 protein levels is proved. The prepared homogeneous polysaccharide can improve the intestinal barrier function, provides a new strategy for treating diarrhea, constipation or colon diseases, and has important clinical value.

The invention belongs to the technical field of medicines, and particularly relates to oral liquid for promoting digestion, relieving indigestion and supplementing calcium, iron and zinc and a preparation method thereof. Comprising the following steps: extracting and purifying rorippa formosana polysaccharide; preparing an auxiliary digestion promoting agent; preparing an aromatic digestion promoting agent; and compounding the oral liquid. The preparation method comprises the following steps: extracting rorippa formosana crude polysaccharide from rorippa formosana through water extraction and alcohol precipitation, then carrying out chromatography on the rorippa formosana crude polysaccharide by using a DEAE cellulose DE-52 column to prepare rorippa formosana polysaccharide, acetylating the rorippa formosana polysaccharide, and compounding with a mixed concentrated solution obtained by water extraction and concentration of hawthorn, coix seed and Chinese yam to obtain the rorippa formosana polysaccharide compound. The prepared auxiliary digestion promoting agent is applied to the oral liquid, so that the effects of promoting digestion and relieving stasis can be enhanced, and the absorption and utilization of the body on effective components of food can be promoted, thereby effectively improving and treating the dyspepsia problem of the human body, improving the appetite of children, and avoiding the phenomena of malnutrition and immunity decline caused by dyspepsia and poor appetite.

The invention provides a polypeptide medicine for treating osteoporosis and a preparation method of the polypeptide medicine, and belongs to the technical field of biological medicine, and the polypeptide medicine comprises the following components: Pleurotus niger peptide, soybean meal protein peptide, a Chinese sumac leaf-broussonetia papyrifera leaf extract, a ulmus pumila leaf-Sophora japonica leaf extract and the like. The method comprises the following steps: sequentially fermenting Pleurotus tuber-regium through candida utilis and lactobacillus plantarum, carrying out enzymolysis through ficin protease, and purifying through a G-50 sephadex column to obtain the Pleurotus tuber-regium peptide. The soybean meal protein peptide is prepared by sequentially carrying out enzymolysis on soybean meal through cellulase, trypsin and bromelain and purifying through an LH-60 sephadex column. The Chinese sumac leaves and the paper mulberry leaves are extracted through an ethanol aqueous solution and purified through a DEAE cellulose column, and the Chinese sumac leaf-paper mulberry leaf extract is prepared. The ulmus pumila leaves and the sophora japonica leaves are sequentially subjected to enzymolysis through cellulase, pepsin and ficin, and purification is conducted through a 732 cation exchange resin column to prepare the ulmus pumila leaf-sophora japonica leaf extract. The components are compounded, so that the bone resorption process is blocked, bone formation and mineralization are strengthened, and osteoporosis is improved.

The invention discloses a liquidambar formosana hance acidic polysaccharide LFP-2 as well as a preparation method and application thereof, and belongs to the field of biological medicines. According to the method, a method for extracting the liquidambar formosana hance polysaccharide by boiling water and precipitating the liquidambar formosana hance polysaccharide by ethanol is adopted, a DEAE cellulose column purification technology is combined, and the principle that macromolecular polysaccharide is insoluble in an organic solvent is utilized, so that the separation of the liquidambar formosana hance acidic polysaccharide is successfully realized, and the liquidambar formosana hance acidic polysaccharide LFP-2 with anti-osteosarcoma activity is obtained; the monosaccharide of the liquidambar formosana hance acidic polysaccharide LFP-2 is prepared from the following components in mole percent: 6.84% of rhamnose, 5.33% of arabinose, 3.76% of galactose, 7.02% of glucose and 77.06% of galacturonic acid. Experiments prove that the traditional Chinese medicine composition has a remarkable anti-osteosarcoma effect, and a new treatment method is provided for clinical treatment of osteosarcoma.

The application provides a detection reagent for frozen semen of Huai pig, and first acquires the raw semen, and then carries out the following steps: ammonium sulfate fractionation precipitation, DEAE cellulose filter gel column chromatography, CM Sepharose FastFlow gel chromatography and Sephadex G-100 chromatography to separate and purify the NAGase of the semen of the Huai pig, and monoclonal antibodies JN21 and JN37 are prepared by taking the NAGase as an antigen, and the monoclonal antibodies can specifically recognize the NAGase of the Huai pig. The prepared double-antibody sandwich ELISA kit can rapidly and quantitatively detect the NAGase content in the semen of the Huai pig, and provides a new thought for Huai pig breeding and purification and rejuvenation. The kit is convenient to operate, low in use cost, good in repeatability and suitable for being widely applied.

The invention discloses a fructus liquidambaris acidic polysaccharide LFP-1 as well as a preparation method and application thereof, and belongs to the field of biological medicines. The method for extracting the liquidambar formosana hance polysaccharide through boiling water extraction and ethanol precipitation is adopted, a DEAE cellulose column purification technology is combined, the principle that macromolecular polysaccharide is insoluble in an organic solvent is utilized, separation of the liquidambar formosana hance acidic polysaccharide is successfully achieved, and the liquidambar formosana hance acidic polysaccharide LFP-1 with anti-breast cancer activity is obtained. The monosaccharide of the liquidambar formosana hance acidic polysaccharide LFP-1 is prepared from the following components in mole percent: 1.66% of fucose, 29.14% of arabinose, 8.63% of rhamnose, 29.85% of galactose, 13.52% of glucose, 2.45% of xylose, 6.98% of mannose, 4.70% of galacturonic acid and 3.07% of glucuronic acid. Experiments prove that the pharmaceutical composition has a remarkable anti-breast cancer effect, and a new treatment method is provided for clinical treatment of breast cancer.