80 results about "DEAE-Cellulose" patented technology

The invention belongs to the field of marine medicines, and relates to a fucosan sulphate, a preparation method thereof and application of the fucosan sulphate in preparing an anti-influenza virus medicine. Polysaccharide with a main chain taking alpha-1,2-D-mannose and beta-1,4-D-glucuronic acid as repetitive units, and with a branched chain of alpha-1,3-L-fucosan sulphate is obtained by virtue of hot-water extraction, calcium chloride precipitation and DEAE-Cellulose chromatographic column purification. The fucosan sulphate prepared by the invention is high in inhibition effect on the influenza virus neuraminidase activities of A H1N1, H5N1 and H3N2, and obvious in protection effect on the dog kidney epithelial cells infected by A H1N1. The fucosan sulphate provided by the invention has the advantages of being rich in raw material source, simple in preparation process, easy to industrialize, high in product water solubility, high in stability, free from toxic and side effects, and the like, and has the prospect of being developed to the anti-influenza virus medicine.

The invention discloses an extraction process and application of peach gum polysaccharide (PGPSD). The process includes: water extraction and alcohol precipitation, protein removal by a Sevage reagent, dialysis, passing of a column with DEAE cellulose as the filler and other steps, thus obtaining finer polysaccharide (PGPSD). The PGPSD can be dissolved in water at room temperature, and is formed by connection of arabinose, mannose and galactose through a glycosidic bond. The peach gum polysaccharide (PGPSD) prepared by the process provided by the invention can reinforce the expression of insulin related transcription factors and glucose degradation key enzyme, has no toxicity to mice, and can significantly lower the blood glucose of diabetic mice.

The invention discloses a preparation method for disaccharide, tetrasccharide and hexaose of chondroitin sulfuric acid, and belongs to the technical field of food. The method comprises the following steps that: the chondroitin sulfuric acid adopted as a raw material is degraded by hyaluronidase, so as to obtain chondroitin sulfuric acid oligosaccharide; through filtering separation by G-25 gel, desalination by G-10, DEAE cellulose 52 anion exchange chromatographic column separation, natural preparation of gel electrophoretic separation, and the like, the disaccharide, tetrasccharide and hexaose of the chondroitin sulfuric acid can be prepared. The invention relates to the preparation method for the disaccharide, the tetrasccharide and the hexaose of the chondroitin sulfuric acid. According to the invention, simultaneous preparation of three types of chondroitin sulfuric acid oligosaccharide can be firstly achieved, and a technical base for industrial preparation for single degree of polymerization chondroitin sulfuric acid oligosaccharide can be provided.

The invention relates to a method for extracting lyceum bararum polysaacharides and the lyceum bararum polysaacharides fat-reducing product. The extraction process comprises: preconditioning the boxthorn fruits by alcoholic solution, extracting the boxthorn fruits after preconditioning by distilled water, then treating the extract using polyamide, DEAE-cellulose(OH-) and an SephadexG-25 exclusion chromatographic column. The lyceum bararum polysaacharides fat-reducing product is made of lyceum bararum polysaacharides as a functional component and its corresponding findings according to traditional technique. In the invention, using water as the extract to extract the lyceum bararum polysaacharides, then separating and purifying by the chromatographic stripping technique.

The invention discloses a preparation method of cherokee rose polysaccharide derivatives with an antitumor activity. The preparation method comprises the following steps: firstly smashing cherokee rose fruit, degreasing, removing oligosaccharide, lixiviating with hot water twice, then mixing extracts, filtrating, concentrating, precipitating and drying so as to obtain crude cherokee rose polysaccharide; adding diethylin ethyl amino ethyl (DEAE)-cellulose 52 to carry out static absorption for 30 minutes; washing with distilled water, filtering, and collecting filtrate so as to obtain the decolored crude cherokee rose polysaccharide; carrying out protein removal on a crude polysaccharide solution by using an enzyme method and a Sevag method; further separating the crude cherokee rose polysaccharide with the DEAE-cellulose 52, collecting, dialyzing, and freeze-drying so as to obtain cherokee rose polysaccharide; and finally, carrying out sulphating, carboxymethylation and hydrochloric acid degradation on the cherokee rose polysaccharide, and carrying out carboxymethylation on degradation products so as to obtain a plurality of cherokee rose polysaccharide derivatives. The plurality of cherokee rose polysaccharide derivatives have in vitro antitumor characteristics, and can be used for inhibiting the growth of three tumor cells, namely, ovarian cancer cells, liver cancer cells and rectal cancer cells.

The present invention relates to a method for preparing high-purity laver phycoerythrin by one-step chromatography, belonging to the preparation technology of the functional ingredients of halobios. Laver is swelled in PBS (1mM, pH6.8) buffer solution with a volume fifty times larger than the volume of the laver and smashed, then 800 watts of ultrasonic waves carry out cell disruption for 800 seconds to produce crude extract, which is precipitated by ammonium sulphate with 45 percent of saturation, microfiltrated via 0.1Mu m of film, ultrafiltered via a 10kDa film, goes through reversed-phase precipitation by ammonium sulphate with 20 percent of saturation and is precipitated and crystallized by ammonium sulphate with 65 percent of saturation under the temperature of 4 DEG C, and one week later, the crude extract passes through a DEAE cellulose-52 anion-exchange chromatography column, is gradiently eluted by PBS (1mM, pH6.8) buffer solutions with different concentrations of NaC1, separated and purified to produce the laver phycoerythrin. The purity (A562nm/A280nm) reaches 5.24, which accords with the reagent-grade requirement.

The invention provides a method for preparing multiple oligosaccharides by separating and purifying Chinese dates. The method comprises the following steps of extracting by a water extraction method, separating by a membrane separation technology, decoloring a strong-base anion exchange resin, and purifying by a DEAE-cellulose (Diethylaminoethyl Cellulose) column and a dextrangel column to obtain four oligosaccharides with edible Chinese dates or imperfect Chinese dates as raw materials. The invention provides a new direction for development of deep processing products of Chinese dates and provides new resources for development of the oligosaccharides. The method, provided by the invention, has the advantages of simple operation process and is suitable for industrial production. The prepared oligosaccharides have higher purity and can be used for functional research and development of function food and medicines.

The process of preparing pollen pini polyose includes the following steps: 1. decocting wall broken pollen pini to extract and filtering; 2. enzymolyzing with pancreatin and cellulose, centrifuging at high speed and ultrafiltering to eliminate impurity; 3. alcohol precipitating the filter residue liquid, and drying the precipitate to obtain coarse pollen pini polyose; 4. eluting coarse pollen pini polyose in DEAE-cellulose column; and 5. nanofiltering, concentrating the filter residue liquid, drying and crushing to obtain single pollen pini polyose. The present invention also discloses the medicinal application in preparing medicine for lowering blood fat, blood pressure and blood sugar, resisting fatigue and raising immunity. The present invention provides one new way for developing and utilizing pollen pini.

The invention discloses a new technology of extracting, separating and purifying 0.1-1 million Da alkali solubility lentinan and a method of molecular weight determination, by taking a mushroom fruit body as raw material, a crude product is obtained by grinding, potash leaching, filtering, concentrating, washing, grading and drying under low temperature, and then the crude product is dissolved and centrifuged, purified by a DEAE cellulose column and absorbed and discolored by ion exchange resin, and filtered to pass through membrane packets of different molecular weights for ultrafiltrating and concentrating. The molecular weight is measured by adopting a GPC laser light scattering gel chromatograph, and the obtained material is respectively put into dialysis bags for dialysis according to the measured molecular weight range, after freeze drying, the alkali solubility lentinan of various components with a molecular weight of 0.1-1 million Da and the content of more than 98 percent is obtained. The method has the advantages of advanced technology, stable quality, high purity, clear goal and easy industrialized production.

The invention discloses a preparation method of an endogenous oily humectant for tobaccos. The preparation method comprises the steps of adding a solvent into a tobacco raw material, extracting by heating, carrying out suction filtration, concentrating filtrate to form paste, settling the paste by using ethanol, regulating the acid value by using NaHCO3 and hydrochloric acid, desalting, and carrying out static microwave vacuum drying on a sediment to obtain crude polysaccharide; primarily purifying the crude polysaccharide, and dialyzing to remove small-molecular impurities after separating and purifying by sequentially using a DEAE-cellulose column, a SephadexG column and a Sephrose column to obtain pure tobacco endogenous polysaccharide; adding a solvent into the tobacco raw material to carry out cold extraction, carrying out suction filtration, concentrating filtrate until no solvent is separated out, adding a solvent to dissolve, carrying out suction filtration, and removing the solvent to obtain tobacco oil substances; and dissolving the pure tobacco endogenous polysaccharide by using a solvent to obtain a solution with the mass concentration of 50%, then, mixing the solution and the tobacco oil substances to 100 parts according to the mass part of (1-99):(1-99), homogenizing at high pressure, and uniformly mixing to obtain the endogenous oily humectant for tobaccos. After the humectant is added into cut tobaccos, the smoking quality of cigarettes can be ensured, and the moisture retention and moisture-proof effects are remarkable.

The invention discloses novel application of laminaria japonica/laminarin extracts. The novel application includes application of the laminaria japonica/laminarin extracts to preparing EV71 resistant medicine. A method for preparing the laminarin extracts can be one of a method (1), a method (2) and a method (3). The method (1) for preparing the extracts from laminaria japonica water includes washing laminaria japonica by the aid of PBS (poly butylenes succinate); boiling the laminaria japonica in double-distilled water; filtering the double-distilled water to obtain boiled juice; centrifuging the boiled juice to obtain supernatant so as to obtain the extracts. The method (2) for preparing aqueous extraction ethanol sediments from laminaria japonica includes washing the laminaria japonica by the aid of PBS; boiling the laminaria japonica in double-distilled water; filtering the double-distilled water to obtain boiled juice; adding anhydrous ethanol into the boiled juice until the concentration of the ethanol reaches 75-95% to obtain mixed liquid; centrifuging the mixed liquid to obtain the aqueous extraction ethanol sediments so as to obtained the extracts. The method (3) for preparing separation components of laminaria japonica by the aid of DEAE (diethyl-aminoethanol) cellulose columns includes separating the aqueous extraction ethanol sediments from one another by the aid of the DEAE cellulose columns; eluting the aqueous extraction ethanol sediments by the aid of NaCl solution; collecting eluted components to obtain the extracts. The novel application has the advantages that experimental bases are provided for clinically treating EV71 infectious diseases by the aid of the laminaria japonica extracts, and the application has a certain guidance significance on developing the EV71 resistant medicine and has important reference value.

The invention discloses a shark glycoprotein, and a preparation method and a use thereof. The preparation method of the shark glycoprotein comprises the following steps: carrying out tissue triturating of shark meat, degreasing, carrying out enzymatic hydrolysis, purifying by allowing the obtained material to undergo DEAE-cellulose-52 column chromatography, Sephadex G-100 column chromatography and reversed-phase high-performance liquid chromatography, condensing, and lyophilizing to obtain the shark glycoprotein. The shark glycoprotein has a molecular weight of about 26.4kDa, a sugar content of 33.65% and a protein content of 66.35%, and contains an O-glycopeptide bond and an N-glycopeptide bond, the monosaccharide of the shark glycoprotein is composed of L-fucose, L-arabinose, L-galactose, D-glucose and D-mannose, and the L-fucose/L-arabinose/L-galactose/D-glucose/D-mannose ratio of 1.00:1.53:7.27:9.07:2.09; and the protein of the shark glycoprotein is composed of seventeen amino acids. The shark glycoprotein can be used as a neovascular inhibitor clinically, can be used for treating diseases comprising tumors and the like, and has a wide market prospect.

The invention discloses a method for extracting a raspberry polysaccharides component having function for removing free radical, which relates to the technical field of traditional Chinese medicine extraction. The method comprises the following steps: 1) cleaning; 2) drying and crushing; 3) extracting; 4) dissolving 5) performing primary purifying; 6) performing secondary purifying; and 7) drying. The method uses an alcohol extracting-water precipitating method for increasing the extraction rate of the polysaccharides component and performing crude separation on the impurity, at the same time, the addition of an extraction-promotion agent can obviously increase the extraction rate of the polysaccharides component, so that the extraction rate can reach 6.52%; a polyamide column and a DEAE-cellulose anion column are used for two-time purification, the purity of the polysaccharides component can be effectively increased, and the purity can reach more than 80%.

The invention discloses a preparation method for high-activity lumbrukinase. The method comprises the following steps: (1) cleaning earthworms, adding a phosphate buffer solution (PBS), carrying out triturating and filtering, taking the filtrate for centrifugation so as to obtain a supernatant; (2) carrying out ultrafiltration to obtain a crude enzyme solution; (3) carrying out fractional precipitation with ammonium sulfate and dissolving the precipitates with PBS to obtain an enzyme solution; (4) purifying the enzyme solution with Sephadex G-25 column; (5) purifying the enzyme solution with DEAE-cellulose column so as to obtain lumbrukinase. According to the invention, earthworms are fed by dry powder of kitchen waste and then lumbrukinase is extracted from the earthworms, thereby realizing resource utilization and transfer of added value of kitchen waste; conventional separating and purifying methods for enzyme are improved and optimized, and enzyme specific activity of lumbrukinasethat is extracted and purified by the method provided in the invention reaches 42000 U/mg, meeting requirements for enzyme for pharmaceutical purposes. The method provided in the invention has the characteristics of simple operation and low cost, and is easy for expanded industrial production.

The method for preparing powder and injection preparations of echinacea polysaccharides comprises the following steps of: adding water into echinacea purpurea herb, boiling, extracting clear solution and decoloring; concentrating and performing ethanol precipitation; centrifuging precipitates; drying, grinding and screening to obtain crude polysaccharides; adding water into the crude polysaccharides, filtering and swelling; centrifuging, and taking supernatant; adding Sevage reagent into the supernatant to remove albumens, and dialyzing the solution by using deionized water; performing DEAE-Cellulose(DE-32) column chromatography; and eluting, concentrating, performing ethanol precipitation, centrifuging, ethanol washing and vacuum drying to obtain the powder and injection preparations of echinacea purpurea polysaccharides. The powder and injection preparations of echinacea purpurea polysaccharides can effectively enhance the immunity of livestock and poultry and improve the immunologic function of organisms.

The invention discloses an extraction method of active components of lycium barbarum. According to the method, lycium barbarum is subjected to freezing and drying at first and then subjected to ultrasonic aqueous extraction, and then filtering is conducted to obtain an aqueous extraction filtrate and aqueous extraction dregs; the aqueous extraction dregs are subjected to microwave ultrasonic extraction to obtain an ethanol extraction mixture I; the aqueous extraction filtrate is subjected to ultrasonic alcohol extraction to obtain an ethanol extraction mixture II; the ethanol extraction mixture I and the ethanol extraction mixture II are filtered to obtain filtrates respectively, the filtrates are mixed and concentrated to obtain a concentrated liquid, the concentrated liquid is extractedby ethyl acetate, and through column chromatography on silica gel and drying, lycium barbarum flavone is obtained; obtained filtration dregs are mixed and then purified by means of DEAE-cellulose column chromatography, and drying is conducted to obtain lycium barbarum polysaccharide; concentrated dregs are subjected to reflux, concentration, macroporous resin chromatography and drying to obtain lycium barbarum coloring matter. The final extraction rates of lycium barbarum flavone, lycium barbarum polysaccharide and lycium barbarum coloring matter are high, the utilization rate of lycium barbarum is greatly increased, the extraction time is shortened at the same time, and the production cost is remarkably reduced.

The invention discloses preparation and activity detection methods of fucoidan components in hizikia fusiformis. According to the key points of the technical scheme, the preparation method comprises the steps that the hizikia fusiformis is washed, dried and crushed, then ethanol is added, reflux degreasing and stirring filtration are conducted, degreased algae powder is extracted repeatedly for three times through a calcium chloride solution, and filtrate is merged to obtain a fucoidan extracting solution; the extracting solution is subjected to depressurizing distillation and concentrated toone fifth of the original volume; anhydrous ethanol is added into a concentrated solution, still standing for the night is conducted, and after centrifugal treatment and cleaning through the anhydrousethanol, vacuum drying is conducted to obtain hizikia fusiformis fucoidan; the hizikia fusiformis fucoidan is dissolved in distilled water, separated through DEAE-cellulose ion exchange column chromatography, eluted with the distilled water and then eluted with 0.1 M of NaCl, 0.3 M of NaCl, 0.5 M of NaCl and 1 M of NaCl, an obtained product is purified through Sepharose CL-6B column chromatography and eluted with NaCl, eluant is analyzed through a phenol-sulfuric acid method, and suitable fractions are collected for preparation. The preparation method has the advantage that preparation of hizikia fusiformis fucoidan components is easy and convenient to operate.

The invention belongs to the technical field of medicines and healthcare food, and particularly relates to radix morindae officinalis saccharide polymer as well as a preparation method and application thereof in preparing a medicine for controlling osteoporosis and/or rheumatism or healthcare food for functional food. A saccharide polymer pure product is obtained by taking radix morindae officinalis as a raw material through combination of water extraction and alcohol precipitation and alkaline extraction and alcohol precipitation, protein removal adopting a Sevag method, DEAE cellulose column chromatography, Sephacryl column chromatograph and the like. The radix morindae officinalis saccharide polymer has a remarkable effect in resisting osteoporosis. Besides, the saccharide polymer pure product obtained by the invention is displayed to have effects of promoting osteocytes to proliferate, differentiate, mineralize and regulate up expression amount of osteogenesis-related genes in bone formation experiment in vitro, is indicated to have the effect of promoting bone forming activity, and can provide basis for application in the fields of healthcare products, medicines and the like.

The invention discloses an abelmoschus manihot stem and leaf polysaccharide and a preparation method and an application thereof. The preparation method comprises the steps: extracting, carrying out ethanol precipitation, carrying out chromatography purification with a macroporous adsorption resin column, separating with a hollow cellulose membrane, carrying out chromatography separation with a DEAE cellulose resin column, collecting target fractions, carrying out desalting treatment, freeze-drying, and thus obtaining the abelmoschus manihot stem and leaf polysaccharide. The abelmoschus manihot stem and leaf polysaccharide has the weight-average molecular weight of 13821 Da and is composed of rhamnose, mannose, galactose, galacturonic acid, glucose and arabinose, and the molar ratio of rhamnose to mannose to galactose to galacturonic acid to glucose to arabinose is 1 to 0.53 to 0.12 to 0.05 to 0.16 to 0.03; the abelmoschus manihot stem and leaf polysaccharide has a good immune enhancing activity, and can be used for development of immune enhancing drugs and health food. Stem and leaf waste resources produced in a production process of abelmoschus manihot medicinal materials are used as raw materials, the polysaccharide substance having the immune activity is separated, and a basis is laid for cyclic utilization of the traditional Chinese medicine resources.

The invention discloses a method for extracting and purifying anti-hypoxic polysaccharides from Qaidam agaricus bitorquis. The method mainly comprises the following steps: extracting crude polysaccharides by an ultrasonic method, discoloring the crude polysaccharides with activated carbon, purifying and eluting various single components by DEAE-52 cellulose column chromatography, removing small molecule substances by utilizing a dialysis membrane, and finally performing rotary evaporation and concentration on the various single components, thereby obtaining the purified single components of polysaccharides. The method has the advantages of high efficiency and high yield, pigment macromolecular substances, proteins and other impurities can be effectively removed, adsorbable ionic substancesare separated by the DEAE cellulose column chromatography, and neutral polysaccharides can smoothly flow out, so that the crude products are removed to obtain refined products, and the separation aimis achieved. The yield of fruiting body crude polysaccharides subjected to ultrasonic extraction reaches 128.25 mg/g; the discoloring ratio of the discolored crude polysaccharides by activated carbonis 76.5%; 3-4 single components of polysaccharides can be separated by the DEAE-52 cellulose column chromatography. The purification degree of the pure polysaccharides of Qaidam agaricus bitorquis can be improved and increased, and the finished polysaccharides derived from the Qaidam agaricus bitorquis at high purity can be obtained.

The invention discloses a lipoteichoic acid from clostridium butyricum and an application thereof in adjusting an immune response of livestock and poultry. The lipoteichoic acid is obtained by the steps of firstly preparing a TX-114 solution with a mass percentage of 1.5-2.5% for use; then layering 8-16 mL of a clostridium butyricum solution cultured for 45-50 h by centrifugation to obtain wet weight of clostridium butyricum; adding the TX-114 solution; centrifuging to obtain a crude extract of lipoteichoic acid; and then separating through column chromatography on a DEAE-cellulose chromatographic column. The lipoteichoic acid can improve intestinal immune functions of the livestock and poultry, suppress adhesion and attachment of pathogenic bacteria on surfaces of intestinal mucosal cells, reduce incidence of intestinal infectious diseases and increase economic benefits of livestock and poultry industry.

The invention discloses passion fruit peel acidic polysaccharide as well as a preparation method and application thereof, and belongs to the technical field of plant extraction and medicines. The preparation method of the passion fruit peel acidic polysaccharide comprises the following steps: crushing passion fruit peel, adding ethanol, performing condensing, performing refluxing and extracting, taking filter residues, performing drying, adding water, performing soaking and extracting, combining extracting solutions, performing concentrating and cooling, adding 95% ethanol, carrying out alcohol precipitation treatment, taking precipitate, and performing centrifuging to obtain crude passion fruit peel polysaccharide; carrying out DEAE cellulose ion exchange column chromatography, performing eluting with distilled water and a NaCl solution in sequence, and performing separating and purifying to obtain the passion fruit peel acidic polysaccharide. The passion fruit peel acidic polysaccharide prepared by the method has hypoglycemic activity, and can be used as a medicine for treating diabetes mellitus.

The invention provides extraction of salt-resistant protease and an application method for shortening fish sauce fermentation time.Penicillium citrinum YL-1 screened from fish sauce fermentation liquid and used for producing salt-resistant protease is adopted, rough protease liquid obtained through fermentation is subjected to precipitation and dialysis with ammonium sulfate and subjected to chromatography with a DEAE cellulose-52 ion exchange column so that three-step purification can be achieved, and obtained protease has the total activity of 19940 U, the total protein of 180.17 mg, the specific activity increased to 110.68 U/mg from 23.74 U/mg, the purification multiple of 4.66 times and the total recovery rate of 48.7%.Serine type protease with protease molecular amount of 32 KDa is measured through an SDS-PAGE electrophoresis method.The protease is good in salt resistance and low in production cost; by adding the protease in the primary stage of fish sauce fermentation, the fish sauce fermentation speed can be accelerated, the fish sauce flavor can not be influenced or changed, and enzymic preparations for shortening fish sauce fermentation time can be developed.

The invention discloses a preparation method of edible and medicinal hericium erinaceus dry powder, and relates to the technical field of edible and medicinal product processing. Hericium erinaceus isheated and pressurized before extraction to soften and destroy the cell wall structure, and therefore leaching of polysaccharide components during subsequent extraction is facilitated; enzymolysis activity of the compound enzyme is improved by adding an activating agent, so that the extraction rate of hericium erinaceus polysaccharide is improved while the extraction time is shortened; and an alcohol precipitation method is combined with DEAE cellulose column chromatography to separate and purify the extract, so that the purity of hericium erinaceus polysaccharide in the product is improved.

The invention discloses a glycopolymer in achyranthes bidentata as well as a preparation method and application thereof. In the method, the achyranthes bidentata is used as a raw material, and is subjected to water-extraction alcohol precipitation and alkaline-extraction alcohol precipitation, so that polymers at all the parts of the achyranthes bidentata are obtained. The extraction method is implemented under a relatively gentle condition, and components of the glycopolymer are intactly preserved. The achyranthes bidentata glycopolymer has a remarkable effect at the aspect of anti-osteoporosis symptom; in addition, a glycopolymer pure product is obtained through separation and purification processes including Sevag method deproteinization, DEAE cellulose column chromatography, Sephacryl column chromatography and the like, and structural analysis shows that the achyranthes bidentata glycopolymer obtained through the method comprises polyose and oligose. Through the adoption of the achyranthes bidentata glycopolymer as well as the preparation method and application thereof, a basis is provided for the application of the achyranthes bidentata glycopolymer in the fields of health care products, medicines and the like.

The invention discloses a method for separation and purification of crocin and crocetion from gardenia yellow pigment. The method comprises the following steps: gardenia yellow pigment with the color value of 170-200A is taken as the raw material and diluted into a gardenia yellow water solution with the color value of 5A; adding acid to adjust the pH to be 5-6; a D-151 resin column is adopted to extract crocin from the gardenia yellow pigment; a DEAE-cellulose adsorption column is adopted to refine the crude extracted crocin to prepare crocin powder with the purity of 79-82%, wherein the yield of crocin is 36.8-40%; after adding ethanol into a residual liquid of the crude extracted crocin and the refined crocin, adding ethyl acetate for extraction so as to prepare crocetion with the purity of 69.5-75%, wherein the yield of crocetion is 20-26%. The method provided by the invention starts from the raw material of gardenia yellow pigment, is simple in operation process, high in separation and purification efficiency and large in operation volume, and can realize industrial large-scale production.