25 results about "Animal brain" patented technology

This invention involves, among other things, elements that stimulate an immune response. Which includes: a) one or more antigens of Avibacterium paragallinarum and One or more antigens of avian brain and spinal cord rhizomatous virus and one antigen. or more of avian porcine ... Furthermore, this invention relates to a method for immunizing animals which includes administration. The animal drug contains components that stimulate the immune response of this invention; moreover, This invention relates to a method of treating or preventing clinical signs caused by Avibactericum. Paragallinarum, a viral infection that causes encephalitis and spinal cord inflammation in birds and avian pox virus in animals. Essential, a method that involves administering medication to animals in effective dosages as a form of treatment. The components that stimulate an immune response are consistent with this invention;

The invention relates to combination or composition comprising: (i) nicotinic acid or a derivative thereof, and (ii) a fatty acid or a derivative thereof selected from tetradecylthioacetic acid 5 (TTA) or a derivative thereof. The invention further relates to a pharmaceutical or nutritional combination or composition for use in a method of preventing or treating mitochondrial diseases, inflammatory diseases, metabolic disorders, neurodegenerative diseases and / or aging of an animal, and / or a method of increasing brain metabolism of an animal, wherein said pharmaceutical or nutritional 10 combination or composition is administered to said animal, and wherein said pharmaceutical or nutritional combination or composition comprises: (i) nicotinic acid or a derivative thereof, and (ii) a fatty acid or a derivative thereof selected from tetradecylthioacetic acid (TTA) or a derivative thereof.

The present invention relates to a method and equipment to record brainwave signals and / or behavioral response in a mammal, analyze recorded signals or behavioral response for parameters, and determine whether the mammal was in an altered mental state at the time of brainwave signal recording. The record signals are analyzed for power, onset time, modulation of magnitude, accumulation, or persistence, modulation of intrinsic timescale, or changes in phase locking and population dynamics. The behavioral response is time to eye closure after an air puff into the eye. The recorded signals and the behavioral response are biomarkers that can be independently detected for determination of an altered mental state in a mammal.

This invention relates to the field of animal experimental equipment technology. It discloses a stereotactic injection device for small animal brains, comprising a main structure, a limiting structure, and a head-fixing structure. The limiting structure is fixedly mounted on the main structure, and the head-fixing structure is fixedly mounted on the limiting structure. This invention allows for easy mounting on a tabletop using the main structure. The limiting structure can limit the animal's body position, making its head more stable, and allows for adjustment of the animal's position during experiments according to usage needs. The limiting unit can also be used to elevate the animal's body. The head-fixing structure allows for adjustment and fixation of the head of animals of stationary size, enhancing overall practicality.

The utility model discloses a kind of electromagnetic shielding's mouse brain deep electrical stimulation and collection device, including protective shell and shielding shell, protective shell bottom is equipped with opening, top is equipped with connecting port, opening is fixedly adhered with animal skull surface, electrode main body, adapter terminal wire and USB conversion interface connected in sequence are equipped in protective shell, electrode main body implants in animal brain through opening;Shielding shell is inserted with connecting port, shielding shell is inserted with connecting port in, USB interface and preamplifier are equipped in shielding shell, USB interface connects USB conversion interface, preamplifier is connected host computer by data line and passes out shielding shell.The utility model, the production and composition of electrode are modularized split, standardization production and surgical implantation process;Modularization installed USB conversion interface hardware cost is very small, improve the Omnetics interface utilization of host computer;USB conversion interface and electrode main body between using adapter terminal wire, namely soft line connection, can avoid the screw drop caused by multiple plug and unplug, prolongs the implantation time of electrode.

The invention belongs to the technical field of animal model construction, and discloses a construction method and application of a mouse encephaledema model. The construction method of the encephaledema model is simple and convenient to operate, simple and easy to repeat, stable in injury, high in success rate and low in death rate, and the encephaledema model can be obtained only by applying 0.4-0.6 W ultrasonic stimulation to the brain of an animal. Parameters can be adjusted according to actual needs, so that the damage degree is changed, the research purpose is met, and high controllability is achieved. The construction method provides a new research model for in-vivo research of the occurrence and development process and mechanism of encephaledema.

The application discloses a kind of multi-mode optical fiber slow ring scanning endoscopic imaging method and device, comprising: long-term implant, pluggable imaging probe and driving mechanism;The long-term implant is rigid transparent tube with bottom end sealed and upper end opened, for forming long-term indwelling guide channel in organism and preventing body fluid from entering lumen;The pluggable imaging probe includes optical fiber and lateral emission element fixed to the end of optical fiber, can be inserted and extracted long-term implant, and obtains the lateral imaging signal of peritubular tissue when being inserted;The driving mechanism is used to drive the imaging probe to generate rotary motion around its own axis and reciprocating displacement along the axial direction, form slow ring scanning scanning track, realize large field of view lateral imaging of peritubular tissue.The application can provide more reliable imaging means for biomedical research and minimally invasive diagnosis and treatment, and is suitable for repeated, stable and large field of view lateral imaging observation of deep tissue in animal brain tissue and other organisms.

The application provides an animal brain metabolite identification method based on mass spectrometry imaging technology and application, and establishes a mouse brain tissue slice endogenous metabolite DESI-MSI analysis method based on mass spectrometry imaging technology, which comprises the following steps: after preparing metabolite control sample imaging samples and brain tissue slice imaging samples, multiple animal brain tissues are attached to the same glass slide, mass spectrometry imaging condition optimization is carried out by using the control sample imaging samples, and mass spectrometry imaging analysis is carried out by using the brain tissue slice imaging samples; the image of the biological sample is divided into multiple structure regions in combination with tissue morphological characteristics; for the structure regions, multivariate statistical analysis is carried out to obtain different metabolites in different structure regions; metabolite identification is carried out based on parent ions and characteristic daughter ion profiles; and the method is used for disease mechanism research and drug intervention effect evaluation on diseases.

The invention relates to the technical field of animal brain tissue sampling, in particular to a low-temperature perfusion device which is characterized in that a recycling groove is formed in the edge of an operation plate, the bottom face of the recycling groove is hollowed out, a binding plate and two stacked low-temperature perfusion assemblies are arranged above the operation plate, and the recycling assembly is arranged in an operation frame; and an operation cooling plate is mounted below the binding plate. The using method of the low-temperature perfusion device comprises the steps that after the limb binding pieces are adjusted, an experimental animal is bound to the upper portion of the binding plate, the two low-temperature perfusion assemblies are filled with a PBS solution and a PFA solution, and then the two low-temperature perfusion assemblies are controlled to conduct PBS solution and PFA solution perfusion on the experimental animal. According to the low-temperature perfusion device, the perfusion function, the low-temperature function and the toxic waste liquid recovery function are integrated, the overall use efficiency of the low-temperature perfusion device is higher, the safety of operators and the environment are better protected, and more importantly, perfusion can be accurately conducted at the small liquid perfusion speed through the ingenious design of the low-temperature perfusion assembly.

The invention relates to an animal brain positioning injection device which comprises a machine base, a mechanical arm, an injection mechanism and a brain fixing mechanism. The mechanical arm comprises a first operating arm, a second operating arm and a third operating arm which can form an XYZ three-axis coordinate system, and the second operating arm is rotatably pivoted and fixed with the first operating arm through a first rotating shaft; the injection mechanism is fixedly mounted on the second operating arm; the brain fixing mechanism comprises a fixing frame base and a brain fixing frame, the fixing frame base is rotatably and fixedly connected with the machine base in a pivoted mode through a second rotating shaft, the axis of the first rotating shaft and the axis of the second rotating shaft can intersect at an intersection point, the axis of an injection needle of the micro-injector penetrates through the intersection point, and the brain fixing frame is fixed to the brain fixing frame. According to the animal brain positioning injection device, multi-angle needle insertion can be achieved, the needle head can be aligned to the position close to the origin of coordinates of the fonfonis of the mouse brain, and therefore the time for positioning the origin coordinates can be shortened, and the positioning efficiency is improved.

The invention provides a novel irradiation fresh-keeping method for brain tissues. The novel irradiation fresh-keeping method comprises the following steps: 1) providing irradiation equipment; 2) obtaining animal brain tissues; 3) the animal brain tissue is placed in a radiation source to be irradiated, the irradiation dose is 1 kGy to 100 kGy, and after irradiation, a fixing agent does not need to be used for fixation; (4) dyeing the treated brain tissue; 5) slicing the animal brain tissue; (6) dehydrating and sealing the sliced animal brain tissue; and 7) performing imaging observation on the brain tissue sample. According to the method, a traditional irradiation preservation technology is applied to fixed preservation of the brain tissue for the first time, so that brain tissue degradation and structural damage caused by enzyme in the brain tissue are inhibited. According to the technology, the structure of the brain tissue sample can still be kept complete without adding a chemical reagent, enough fresh brain tissue samples are expected to be provided for brain science research, especially human brain research, and technical support is provided for brain science research.

This invention discloses a liposomal curcumin composition for regulating cardiovascular and cerebrovascular health and its application, belonging to the field of food and health food technology. The core components of the composition include: 60-80nm liposomal curcumin, prebiotics (2-8g / serving), cardamom extract (10-60mg / serving), animal brain-derived neurotrophic microcapsule powder (200mg daily), and one or more of the following: nattokinase, phytosterol esters (phytosterol content ≥90%, 500-2000mg / serving), coenzyme Q10, and hawthorn extract (total flavonoids ≥5%, 100-400mg / serving). This invention constructs a five-fold protective system through the synergistic effect of multiple components, encompassing intestinal regulation, blood purification, vascular care, myocardial enhancement, and brain nerve nourishment, achieving integrated regulation of cardiovascular and cerebrovascular health. Its bioavailability is more than 12 times higher than that of ordinary curcumin, and it achieves a comprehensive improvement rate of 86.7% for sub-health conditions of cardiovascular and cerebrovascular health in middle-aged and elderly individuals. It can be prepared as an oral liquid, solid beverage, soft capsule, or tablet, possessing extremely high application value.

This invention discloses a non-human primate visual-spatial working memory testing cage. This invention uses a non-human primate visual-spatial working memory testing cage to measure the learning, discrimination, and reversal abilities, or cognitive and memory abilities, of non-human primates, providing a more comprehensive evaluation of changes in animal brain function and behavior. It can provide a model evaluation tool for central nervous system diseases such as Alzheimer's disease and stroke, and offer new methods for disease research and new drug development.

The present application relates to a kind of Parkinson's disease animal model construction method, which is directly injected into the brain of animal by MPTP in the substantia nigra, and then the characteristic index score of animal is judged according to the characteristic index score standard, one Parkinson's disease animal model only needs to be injected once by MPTP for one animal, which is simple in operation process and short in time, greatly reduces the difficulty of modeling and animal care, which can achieve the purpose of modeling with a small amount of MPTP, thereby improving the modeling efficiency, and since the neurotoxicity response induced by MPTP is irreversible, MPTP is directly injected into the brain to act, which can also avoid the hindrance when MPTP is transmitted to the substantia nigra from other tissues of animal, thereby improving the success rate and stability of modeling.

This utility model discloses a multifunctional animal craniocerebral injury device, including a bottom support plate, an L-shaped support plate welded to the bottom support plate, a force-applying mechanism slidably connected to the L-shaped support plate, and a striking pin installed below the force-applying mechanism; it also includes a lifting and releasing mechanism, which can raise and release the force-applying mechanism to allow it to fall freely. This relates to the technical field of craniocerebral injury devices. By setting up the lifting and releasing mechanism, when the force-applying mechanism is raised, the electric push rod retracts and drives the support rod to rotate, causing the rolling wheel to disengage from the second support ring, thus allowing the force-applying mechanism to descend under the action of gravity, improving ease of use; by setting a spring at the top of the striking rod, when the force-applying mechanism is raised, the spring is compressed, and the spring force drives the force-applying mechanism to descend, thus allowing the striking pin to obtain greater kinetic energy without the striking rod needing to be raised very high.

The invention relates to the field of analytical chemistry and the field of lipidomics research, in particular to a method for omics analysis of acidic glycosphingolipids in animal brain tissues. The group analysis method comprises the following steps: firstly, extracting ganglioside and thioglycoside from animal brain tissues, carrying out C18 solid-phase extraction and enrichment, then carrying out qualitative analysis by using Nano-LC-Chip-Q-TOF-MS, carrying out analysis in combination with a QSRR model, and then carrying out quantitative analysis by using Nano-LC-Chip-QQQ-MS. The group analysis method is based on a Chip technology, combines C18 solid-phase extraction segmented enrichment and a QSRR model, greatly avoids ion inhibition and signal interference, has high resolution, high sensitivity and high accuracy, can accurately distinguish isomeride and same-weight elements, remarkably improves the detection efficiency and the structural identification and unknown substance prediction and quantification capability, and has a good application prospect. The defects of low-abundance component loss, narrow dynamic range and the like in the prior art are overcome.

The application discloses an addictive drug relapse related regulatory gene after drug withdrawal and a screening method thereof. The method comprises the following steps: constructing a drug addiction animal model; performing drug withdrawal treatment on the drug addiction animal model; dividing the drug addiction animal model after the drug withdrawal treatment into three groups, i.e., group A, group B and group C; the group A is a group of addictive drug CPP, the group B is a group of reverse addictive drug CPP, and the group C is a group of food reward CPP; collecting brain tissues of the addictive drug CPP, the reverse addictive drug CPP and the food reward CPP, performing second-generation sequencing, analyzing transcriptome and proteome expression, and obtaining the addictive drug relapse related regulatory gene after drug withdrawal. In the past, the drug addiction mechanism research usually only divides experimental animals into two groups, i.e., a drug group and a saline group, and the application increases environmental variables on the basis of the grouping, so that the related regulatory gene can be more accurately found.

The invention discloses an electrode protective cap for an animal brain-computer interface experiment, which is characterized by comprising a protective base and a protective cover, wherein the protection base is installed on the cranium top of an experimental animal through screws, the implantable electrode interface is located in the center of the protection base, and external threads are arranged on the periphery of the upper end of the protection base and used for achieving sealed connection with the protection cover; the inner periphery of the protective cover is provided with internal threads matched with the external threads, the protective cover is detachably connected with the protective base through threaded fit, the surface of a cover body of the protective cover is covered with a waterproof breathable film, an annular sealing rubber strip is embedded in the edge of the bottom of the cover body, and the sealing rubber strip achieves sealing attachment of the end faces of the protective cover and the protective base in the locked state of the protective cover and the protective base. The effect of protecting the electrode can be achieved through the protection base, the protection upper cover, the sealing ring and the waterproof breathable film, animal scratching and damage to the electrode caused by the external environment are avoided, and meanwhile the scheme that rigid materials and flexible materials are combined is adopted to adapt to the head contours of different experimental animals.

The invention relates to the technical field of brain stereotactic positioning of non-human Linglong animals, and particularly discloses a brain operation stereotactic positioning method for non-human Linglong animals. Comprising the steps of original point titanium nail installation, head fixing and scanning, positioning pre-operation preparation, headstock and operation equipment installation, original point centering, number point marking, positioning hole forming, coordinate verification, sample injection execution, needle withdrawing and the like. According to the method, before an operation, a titanium nail is installed on a skull of an experimental animal to serve as a physical reference point, and then a coordinate value of a positioning point of a target brain region during the operation is calculated according to the physical reference point to carry out trepanning and sample introduction, so that the positioning precision is not influenced by differences among experimental animal individuals and repeated positioning errors of equipment; therefore, the defect of inaccurate positioning of the target brain region is overcome.

The invention discloses a photoacoustic probe for detecting noradrenaline and preparation and application of the photoacoustic probe, the probe is named as QSH-NE, the probe takes 4-(bromomethyl)-2-formyl phenyl pyrrolidine-1-carboxylate as a skeleton to construct a recognition-release bifunctional unit, and sulfur-substituted hemicyanine QSH-OH is selected as a photoacoustic signal report group. The photoacoustic signal activation mechanism depends on that an aldehyde group captures an NE amino group to initiate intramolecular cyclization, then ortho-carbamate is triggered to be cracked to generate 2-hydroxyl, and finally reporter group release and ICT effect recovery are realized through a 1, 6-elimination reaction, so that accurate detection of NE is realized. The probe has the characteristics of high specificity, high sensitivity and quick response to NE, and realizes detection, imaging and drug treatment evaluation of noradrenaline in living cells and living animal brains. According to the technology, the comprehensive performance of NE detection is remarkably improved, and powerful tool support is provided for mechanism research, clinical diagnosis and drug development of depression.

In one aspect, the invention provides a method for active neuronal tracing in postmortem mammalian brain, wherein the method comprises perfusing a mammalian brain with a perfusate using a perfusion device; introducing a tracer into the mammalian brain while the mammalian brain is being perfused with the perfusate; and monitoring movement of the tracer in the mammalian brain.

The application discloses a method for constructing a microwave radiation animal brain injury model based on a medial septum-hippocampus CA3 cholinergic nerve loop regulation, comprising the following steps: setting electromagnetic radiation conditions, and performing electromagnetic radiation treatment on test animals; comparing whether a cholinergic nerve loop between the medial septum and the hippocampus CA3 is activated before and after the electromagnetic radiation treatment; and if the cholinergic nerve loop is activated, constructing an electromagnetic radiation animal brain injury model by using the electromagnetic radiation conditions. The application can accurately construct the electromagnetic radiation animal brain injury model, and increases the possibility of elucidating the molecular mechanism of the electromagnetic radiation brain injury effect and the research and development of prevention and treatment drugs.

This invention discloses an integrated fixation system for animal brain-computer interfaces, belonging to the fields of biomedical engineering and animal robotics. It includes a protective helmet that fits snugly against the front of the target animal's head via cushioning pads; an electrode shield positioned above the electrode implantation points on the target animal; and a flexible circuit board disposed inside the protective helmet. The electrode implantation points on the electrode shield are connected to the flexible circuit board via short pins, and the flexible circuit board is connected to a main control device mounted on the back of the target animal via a data bus. This invention absorbs and isolates external impacts step by step, effectively protecting the implanted electrodes and ensuring their long-term stability in place.

The invention discloses a method for constructing an animal brain injury model based on primary auditory cortex-basal lateral amygdaloid nucleus neural circuit regulation, which comprises the following steps: S1, setting electromagnetic radiation conditions including static magnetic field parameters and microwave parameters, and carrying out electromagnetic radiation treatment on a test animal; s2, performing positioning injection on the primary auditory cortical brain region of the test animal by adopting a consequent non-transsynaptic neurophilic virus, and performing positioning injection on the amygdala brain region on the outer side of the basal of the test animal by adopting a retrograde non-transsynaptic neurophilic virus, according to the fluorescence signals of the primary auditory corticobrain region and the basal lateral amygdala brain region, counting nerve fibers projected to the basal lateral amygdala brain region from the primary auditory corticobrain region and nerve fibers projected to the primary auditory corticobrain region from the basal lateral amygdala brain region in a retrograde manner; and if the reduction of the projection amount of the nerve fibers before and after the electromagnetic radiation treatment has statistical significance, constructing the electromagnetic radiation animal brain injury model by utilizing the electromagnetic radiation condition in the S1. The invention provides a new model for explaining a neural mechanism of brain injury caused by electromagnetic field exposure.

The utility model provides a brain tissue extractor for an experimental animal. The brain tissue extractor comprises a cutting device and a driving device, the cutting device comprises a base and two cutting mechanisms. Each cutting mechanism comprises a rotating shaft, a cutting blade, an adjusting assembly and a driving part. The rotating shaft is rotatably installed on the base, the two cutting blades are coaxially installed on the rotating shaft through an adjusting assembly, and the adjusting assembly can adjust the positions of the cutting blades in the length direction of the rotating shaft; the driving piece can drive the rotating shaft to rotate; wherein the two rotating shafts are arranged in a mutually perpendicular and staggered mode, and a rectangular shape is defined by the four cutting blades; the driving device can drive the cutting device to move in the cutting direction of the cutting blade. The skull of the test animal is cut by the four cutting blades which form the rectangle, so that a rectangular opening can be cut on the skull of the test animal. Therefore, a hand-held angle grinder is replaced for opening, so that the risk of operators can be effectively reduced, and more time and labor are saved.