48 results about "Animal brain" patented technology

The invention relates to a self-immobilized hydrogen peroxide imaging tracer agent as well as a preparation method and application thereof, and belongs to the technical field of nuclear medicine. The invention provides a series of self-fixing type hydrogen peroxide imaging tracers based on aryl boric acid ester. The tracers comprise [18F] HYAS, [18F] HYAT and the like. The series of tracers are high in self-fixing capacity and can react with H2O2 to form QM intermediates, so that the QM intermediates and nucleophilic reagents in biomacromolecules form covalent bonds; in addition, the series of tracers have the physicochemical property of being capable of penetrating through the blood brain barrier; meanwhile, experiments show that the series of tracers can distinguish tissues with different H2O2 concentrations in vivo, and in animal brains treated by 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine, ingestion increase of the series of tracers can be observed, and the series of tracers have very strong H2O2 responsiveness. In conclusion, the series of tracers have great application prospects in non-invasive imaging of H2O2 in the brain.

This invention involves, among other things, elements that stimulate an immune response. Which includes: a) one or more antigens of Avibacterium paragallinarum and One or more antigens of avian brain and spinal cord rhizomatous virus and one antigen. or more of avian porcine ... Furthermore, this invention relates to a method for immunizing animals which includes administration. The animal drug contains components that stimulate the immune response of this invention; moreover, This invention relates to a method of treating or preventing clinical signs caused by Avibactericum. Paragallinarum, a viral infection that causes encephalitis and spinal cord inflammation in birds and avian pox virus in animals. Essential, a method that involves administering medication to animals in effective dosages as a form of treatment. The components that stimulate an immune response are consistent with this invention;

The invention relates to combination or composition comprising: (i) nicotinic acid or a derivative thereof, and (ii) a fatty acid or a derivative thereof selected from tetradecylthioacetic acid 5 (TTA) or a derivative thereof. The invention further relates to a pharmaceutical or nutritional combination or composition for use in a method of preventing or treating mitochondrial diseases, inflammatory diseases, metabolic disorders, neurodegenerative diseases and / or aging of an animal, and / or a method of increasing brain metabolism of an animal, wherein said pharmaceutical or nutritional 10 combination or composition is administered to said animal, and wherein said pharmaceutical or nutritional combination or composition comprises: (i) nicotinic acid or a derivative thereof, and (ii) a fatty acid or a derivative thereof selected from tetradecylthioacetic acid (TTA) or a derivative thereof.

The present invention relates to a device and method for monitoring the cerebral blood oxygen saturation. The device comprises a power supply and modulation module, a light source module, a light guide module, an optical detection module, and a data processing and analysis module; under the action of the power supply and modulation module, light emitted by the light source module is transmitted into an area to be monitored within the brain of a test subject by means of a transmission optical fiber of the light guide module; after the light optically interacts with the brain tissue in said area within the brain of the test subject, a portion of the light enters the transmission optical fiber; the transmitted light is received by the optical detection module and processed by the data processing and analysis module to calculate and obtain continuous blood oxygen saturation data. The device and method for monitoring the cerebral blood oxygen saturation of the present invention adopt a spectroscopy method with a relatively direct measurement principle, are designed and developed using an optical fiber recording technology with a deep measurement depth and a minor impact on the activities of the test subject, and thus can be used for real-time blood oxygen saturation monitoring in an animal brain including the deep brain structure.

The present invention relates to a method and equipment to record brainwave signals and / or behavioral response in a mammal, analyze recorded signals or behavioral response for parameters, and determine whether the mammal was in an altered mental state at the time of brainwave signal recording. The record signals are analyzed for power, onset time, modulation of magnitude, accumulation, or persistence, modulation of intrinsic timescale, or changes in phase locking and population dynamics. The behavioral response is time to eye closure after an air puff into the eye. The recorded signals and the behavioral response are biomarkers that can be independently detected for determination of an altered mental state in a mammal.

The invention provides a method and a device for synchronously determining nicotine metabolism and dopamine effect in the brain of a freely moving animal and application of the method and the device. The invention discloses a method for synchronously determining nicotine metabolism and dopamine effect in the brain of a free moving animal. The method comprises the following steps: S1, injecting a dopamine probe virus into an NAc brain region on one side of the animal; s2, embedding a ceramic ferrule, a microdialysis probe sleeve and an abdominal cavity administration catheter; s3, connecting the optical fiber patch cord with the ceramic ferrule, recording the fluorescence intensity, inserting the microdialysis probe into the microdialysis probe sleeve to collect dialysate, and injecting nicotine through the administration catheter after balancing; and S4, determination and data analysis. On the basis of a microdialysis and optical fiber recording system combined technology, space-time synchronous analysis is carried out on nicotine metabolism and dopamine effect in the brain of a free moving animal, independent monitoring of a double-side NAc brain region is realized, and a methodological basis is provided for deeply revealing the physiological action of nicotine.

This invention relates to the field of animal experimental equipment technology. It discloses a stereotactic injection device for small animal brains, comprising a main structure, a limiting structure, and a head-fixing structure. The limiting structure is fixedly mounted on the main structure, and the head-fixing structure is fixedly mounted on the limiting structure. This invention allows for easy mounting on a tabletop using the main structure. The limiting structure can limit the animal's body position, making its head more stable, and allows for adjustment of the animal's position during experiments according to usage needs. The limiting unit can also be used to elevate the animal's body. The head-fixing structure allows for adjustment and fixation of the head of animals of stationary size, enhancing overall practicality.

The present invention discloses an animal bed device based on a cryogenic coil for <supgt;129< / supgt;Xe MRI of the brain. The present invention adjusts in various ways through front and rear positioning rods, a lifting table, and fine-tuning long bolts to make the brain of the experimental animal closely adhere to the cryogenic coil, ensuring a high filling factor of the coil, and thus ensuring a high signal-to-noise ratio of the imaging. The present invention is also provided with a simulation chamber, which restores the relative positions of the cryogenic coil and the animal bed in the cavity of the magnetic resonance scanner in a 1:1 manner, and ensures that the brain of the experimental animal closely adheres to the cryogenic coil when the animal bed enters the cavity of the magnetic resonance scanner without using the conventional laser positioning method. The cryogenic coil simulation component and the cryogenic coil housing simulation part are made of transparent materials, facilitating the observation of the fitting degree between the experimental animal and the cryogenic coil simulation component and adjustment; the lifting table of the present invention also provides a flat surface to support the brain of the animal, preventing the animal from being unable to be properly positioned due to interference from the tracheal intubation during animal positioning.

The present invention provides a method for extracting synaptic vesicles, comprising the following steps: Step 1): Homogenize the mouse brain with a homogenization buffer; Step 2): Perform a first centrifugation to collect the first supernatant; Step 3): Perform a second centrifugation to collect the second supernatant and the precipitate; Step 4): Add ddH2O to the precipitate and homogenize; Step 5): Perform a third centrifugation to collect the third supernatant to obtain the supernatant; Step 6): Collect the second and third supernatants and perform a fourth centrifugation to collect the fourth supernatant; Step 7): Purify the fourth supernatant by Optiprep density gradient centrifugation; Step 8): Select the layer with the highest vesicle content and elute through a column. Through this extraction method, synaptic vesicle samples can be efficiently extracted from ex vivo mammalian brains. The synaptic vesicles have a high content in proteins, above 25%, even above 30%, and a high purity. The present invention also provides the synaptic vesicles extracted by the above extraction method.

The utility model discloses a kind of electromagnetic shielding's mouse brain deep electrical stimulation and collection device, including protective shell and shielding shell, protective shell bottom is equipped with opening, top is equipped with connecting port, opening is fixedly adhered with animal skull surface, electrode main body, adapter terminal wire and USB conversion interface connected in sequence are equipped in protective shell, electrode main body implants in animal brain through opening;Shielding shell is inserted with connecting port, shielding shell is inserted with connecting port in, USB interface and preamplifier are equipped in shielding shell, USB interface connects USB conversion interface, preamplifier is connected host computer by data line and passes out shielding shell.The utility model, the production and composition of electrode are modularized split, standardization production and surgical implantation process;Modularization installed USB conversion interface hardware cost is very small, improve the Omnetics interface utilization of host computer;USB conversion interface and electrode main body between using adapter terminal wire, namely soft line connection, can avoid the screw drop caused by multiple plug and unplug, prolongs the implantation time of electrode.

The invention belongs to the technical field of animal model construction, and discloses a construction method and application of a mouse encephaledema model. The construction method of the encephaledema model is simple and convenient to operate, simple and easy to repeat, stable in injury, high in success rate and low in death rate, and the encephaledema model can be obtained only by applying 0.4-0.6 W ultrasonic stimulation to the brain of an animal. Parameters can be adjusted according to actual needs, so that the damage degree is changed, the research purpose is met, and high controllability is achieved. The construction method provides a new research model for in-vivo research of the occurrence and development process and mechanism of encephaledema.

The application discloses a kind of multi-mode optical fiber slow ring scanning endoscopic imaging method and device, comprising: long-term implant, pluggable imaging probe and driving mechanism;The long-term implant is rigid transparent tube with bottom end sealed and upper end opened, for forming long-term indwelling guide channel in organism and preventing body fluid from entering lumen;The pluggable imaging probe includes optical fiber and lateral emission element fixed to the end of optical fiber, can be inserted and extracted long-term implant, and obtains the lateral imaging signal of peritubular tissue when being inserted;The driving mechanism is used to drive the imaging probe to generate rotary motion around its own axis and reciprocating displacement along the axial direction, form slow ring scanning scanning track, realize large field of view lateral imaging of peritubular tissue.The application can provide more reliable imaging means for biomedical research and minimally invasive diagnosis and treatment, and is suitable for repeated, stable and large field of view lateral imaging observation of deep tissue in animal brain tissue and other organisms.

The application provides an animal brain metabolite identification method based on mass spectrometry imaging technology and application, and establishes a mouse brain tissue slice endogenous metabolite DESI-MSI analysis method based on mass spectrometry imaging technology, which comprises the following steps: after preparing metabolite control sample imaging samples and brain tissue slice imaging samples, multiple animal brain tissues are attached to the same glass slide, mass spectrometry imaging condition optimization is carried out by using the control sample imaging samples, and mass spectrometry imaging analysis is carried out by using the brain tissue slice imaging samples; the image of the biological sample is divided into multiple structure regions in combination with tissue morphological characteristics; for the structure regions, multivariate statistical analysis is carried out to obtain different metabolites in different structure regions; metabolite identification is carried out based on parent ions and characteristic daughter ion profiles; and the method is used for disease mechanism research and drug intervention effect evaluation on diseases.

The invention relates to the technical field of animal brain tissue sampling, in particular to a low-temperature perfusion device which is characterized in that a recycling groove is formed in the edge of an operation plate, the bottom face of the recycling groove is hollowed out, a binding plate and two stacked low-temperature perfusion assemblies are arranged above the operation plate, and the recycling assembly is arranged in an operation frame; and an operation cooling plate is mounted below the binding plate. The using method of the low-temperature perfusion device comprises the steps that after the limb binding pieces are adjusted, an experimental animal is bound to the upper portion of the binding plate, the two low-temperature perfusion assemblies are filled with a PBS solution and a PFA solution, and then the two low-temperature perfusion assemblies are controlled to conduct PBS solution and PFA solution perfusion on the experimental animal. According to the low-temperature perfusion device, the perfusion function, the low-temperature function and the toxic waste liquid recovery function are integrated, the overall use efficiency of the low-temperature perfusion device is higher, the safety of operators and the environment are better protected, and more importantly, perfusion can be accurately conducted at the small liquid perfusion speed through the ingenious design of the low-temperature perfusion assembly.

The utility model discloses a syringe device in an animal brain. The syringe device comprises two microsyringes, an injection push cap, a first fixator, a second fixator and a needle pitch fixator, each of the two microsyringes comprises a needle cylinder, a piston and a needle body, one end, with a piston head, of the piston is arranged in the needle cylinder and can slide on the inner wall of the needle cylinder, the other end of the piston is sleeved with the injection push cap, and the two microsyringes are fixed through the injection push cap and the first fixator. The two needle bodies are arranged in the syringe device, the needle distance fixer fixes the distance between the two needle bodies at a required distance, and the two microsyringes are ensured to be parallel and stable, so that the defect that the existing microsyringes can only inject medicines on one side is greatly improved, and the injection push cap of the syringe device is pushed manually or by other automatic propulsion devices, so that the injection efficiency is greatly improved. Therefore, simultaneous injection in the brains of the two sides of the animal can be realized, experimental operation time is obviously reduced, and animal death rate is reduced.

The invention relates to an animal brain positioning injection device which comprises a machine base, a mechanical arm, an injection mechanism and a brain fixing mechanism. The mechanical arm comprises a first operating arm, a second operating arm and a third operating arm which can form an XYZ three-axis coordinate system, and the second operating arm is rotatably pivoted and fixed with the first operating arm through a first rotating shaft; the injection mechanism is fixedly mounted on the second operating arm; the brain fixing mechanism comprises a fixing frame base and a brain fixing frame, the fixing frame base is rotatably and fixedly connected with the machine base in a pivoted mode through a second rotating shaft, the axis of the first rotating shaft and the axis of the second rotating shaft can intersect at an intersection point, the axis of an injection needle of the micro-injector penetrates through the intersection point, and the brain fixing frame is fixed to the brain fixing frame. According to the animal brain positioning injection device, multi-angle needle insertion can be achieved, the needle head can be aligned to the position close to the origin of coordinates of the fonfonis of the mouse brain, and therefore the time for positioning the origin coordinates can be shortened, and the positioning efficiency is improved.

The invention provides a novel irradiation fresh-keeping method for brain tissues. The novel irradiation fresh-keeping method comprises the following steps: 1) providing irradiation equipment; 2) obtaining animal brain tissues; 3) the animal brain tissue is placed in a radiation source to be irradiated, the irradiation dose is 1 kGy to 100 kGy, and after irradiation, a fixing agent does not need to be used for fixation; (4) dyeing the treated brain tissue; 5) slicing the animal brain tissue; (6) dehydrating and sealing the sliced animal brain tissue; and 7) performing imaging observation on the brain tissue sample. According to the method, a traditional irradiation preservation technology is applied to fixed preservation of the brain tissue for the first time, so that brain tissue degradation and structural damage caused by enzyme in the brain tissue are inhibited. According to the technology, the structure of the brain tissue sample can still be kept complete without adding a chemical reagent, enough fresh brain tissue samples are expected to be provided for brain science research, especially human brain research, and technical support is provided for brain science research.

The invention discloses a chronic continuous drug delivery method in the ventricle of an experimental animal, and belongs to the technical field of animal model construction. The method comprises the following steps: (1) anesthetizing an animal; (2) longitudinally cutting the skin of the parietal bone along the direction of the occipital bone macropore formed by the midpoint of the connecting line of the two eyes; (3) disinfecting the head and removing connective tissues on the surface of the parietal bone; (4) penetrating through the left ventricle by using a brain stereotaxic instrument; (5) simultaneously connecting a chronic drug delivery pump with a catheter, soaking the chronic drug delivery pump in normal saline for 20-28 hours, taking out the chronic drug delivery pump, filling the chronic drug delivery pump with a tested drug, and injecting the tested drug into the catheter; (6) cutting the catheter to a proper length, inserting the far end of the catheter into the left ventricle, fixing, and after the catheter is dried up, remaining the chronic drug delivery pump under the skin of the head along the opening of the skin of the head; and (7) sewing a head opening. According to the method, insufficient drug effect persistence caused by too high drug metabolism speed can be avoided, and in addition, injury to the tested animal due to multiple times of opening and puncturing can be reduced, so that non-drug influence is reduced.

The invention discloses a system for scanning the brain of an animal in any posture. The system is characterized by comprising an animal carrying box, a carrying table, a binocular camera, a PET / CT scanning system, a positioning frame and a computer. At least one surface of the animal carrying box is transparent, and the binocular camera is placed outside the transparent surface and faces the animal carrying box; the carrying platform carries the animal carrying box from below; the positioning frame is an RFS rigid tool and comprises a connecting frame and at least four small balls fixed to different positions of the connecting frame. A PET / CT image processing system and binocular positioning software are stored in the computer. The invention discloses a method for scanning the brain of an animal in any posture. The method comprises a preparation step, a scanning step, a corresponding step, a discrimination step, a coordinate adjustment step and a PET / CT image generation step.

The invention discloses an implantable animal brain-computer interface device and method for a gravity simulation cabin of a lunar base. The device comprises an implantable brain-computer interface module, an animal behavior monitoring module, an environment control module and a data processing and analyzing module. The implantable brain-computer interface module is used for collecting and transmitting a neural signal set of an experimental animal brain; the animal behavior monitoring module is used for collecting and analyzing behavior activities of experimental animals to obtain an animal behavior data set; the environment control module is used for measuring and controlling gravity, temperature, humidity and illumination in the moon gravity simulation cabin to obtain an environment measurement information set; and the data processing and analysis module is used for processing and analyzing the collected environment measurement information set, the neural signal set and the animal behavior data set to obtain an analysis result information set.

To enable highly accurate, simple and rapid detection of the presence of autoantibodies associated with known neurological diseases in a test subject.SOLUTION: An immunochromatographic test kit according to the present invention is a kit for detecting an autoantibody that binds to a receptor protein present in a subject's central nervous system in relation to a neurological disease, and includes a test strip 1 for developing a specimen, and the test strip 1 is provided with a dropping section 2 for dropping the specimen, a labeling section 3 on which a labeled anti-human IgG antibody is disposed, and a detection section 4 on which an antibody that recognizes and binds to the receptor protein is held, which are arranged in this order, and the specimen is a mixture of the cerebrospinal fluid of the subject and an animal brain extract.SELECTED DRAWING: Figure 1

The utility model relates to the technical field of animal modeling, in particular to an animal brain fixing modeling device which comprises a base plate, the left side and the right side of the top of the base plate are fixedly connected with mounting vertical shells, and the front side and the rear side of the bottom of an inner cavity of each mounting vertical shell are fixedly connected with first electric push rods. Supporting sliding plates are connected to the tops of the first electric push rods through bolts, a supporting transverse plate is movably connected to the top between the left supporting sliding plate and the right supporting sliding plate through bearings, and second electric push rods are connected to the front side and the rear side of the left side of the top of the supporting transverse plate through bolts. A pull rod is pulled rightwards to drive a positioning pin to move out of a positioning hole through a connecting sliding plate, a supporting transverse plate is rotated through a rotating disc, the afterbrain of the animal is turned over, the pull rod is loosened, a positioning spring releases elastic force to push the positioning pin to be inserted into the positioning hole, and therefore personnel can conduct experimental observation on the afterbrain of the animal through an opening, and operation is more convenient and faster; and the experiment efficiency is effectively improved.

Brain-temperature-control (BTC) systems for controlling brain temperature via one or more neck arteries, and neck wraps therefore. In some embodiments, the neck wrap includes one or more thermal islands each having one or more fluid-flow-based thermal elements that carry a circulated fluid coolant during brain-cooling operations. In some embodiments, the neck wrap includes one or more sensor islands that each contain one or more sensors that provide one or more feedback signals that a controller of the BTC system uses to control one or more operating parameters of the BTC system. In some embodiments, the BTC system includes one or more sensors external to the neck wrap that provide such feedback signals. In some embodiments, the BTC system provides both brain cooling and brain warming functionalities. Methods, algorithms, and other features of BTC systems are also disclosed.

An animal brain tissue storage box comprises a box body with an opening in the top and a pressing plate matched with the box body, the box body comprises a square bottom plate and side plates arranged on the four sides of the bottom plate, the bottom plate and the side plates define a storage space, the bottom plate is provided with a first scale mark used for measuring the length of brain tissue and a second scale mark used for measuring the width of the brain tissue, and the bottom plate is provided with a second scale mark used for measuring the width of the brain tissue. The side plate is provided with a third scale mark for measuring the height of brain tissue; the pressing plate is arranged in the storage space and can ascend and descend along the side plates. According to the principle, the square box body is large in space, animal brain tissue can be vertically taken and placed, and taking and placing are convenient; the scale marks are arranged on the bottom plate and the side plates, so that the brain tissues can be stored and the sizes of the brain tissues can be measured conveniently and quickly; the pressing plate fixes the brain tissue by virtue of the gravity of the pressing plate, so that the brain tissue cannot be greatly extruded, and the complete form of the brain tissue is ensured; when the brain tissue needs to be cut, the pressing plate is opened, the brain tissue can be aligned in the box body for cutting through the scale marks, and the steps of taking out, positioning and measuring the brain tissue are omitted.

This invention discloses a liposomal curcumin composition for regulating cardiovascular and cerebrovascular health and its application, belonging to the field of food and health food technology. The core components of the composition include: 60-80nm liposomal curcumin, prebiotics (2-8g / serving), cardamom extract (10-60mg / serving), animal brain-derived neurotrophic microcapsule powder (200mg daily), and one or more of the following: nattokinase, phytosterol esters (phytosterol content ≥90%, 500-2000mg / serving), coenzyme Q10, and hawthorn extract (total flavonoids ≥5%, 100-400mg / serving). This invention constructs a five-fold protective system through the synergistic effect of multiple components, encompassing intestinal regulation, blood purification, vascular care, myocardial enhancement, and brain nerve nourishment, achieving integrated regulation of cardiovascular and cerebrovascular health. Its bioavailability is more than 12 times higher than that of ordinary curcumin, and it achieves a comprehensive improvement rate of 86.7% for sub-health conditions of cardiovascular and cerebrovascular health in middle-aged and elderly individuals. It can be prepared as an oral liquid, solid beverage, soft capsule, or tablet, possessing extremely high application value.

The utility model relates to the technical field of arterial occlusion model construction, in particular to a blood vessel ligation tool for arterial occlusion model construction in animal brain.The blood vessel ligation tool comprises a holding part, a supporting part connected with the holding part and a separating threading part connected with the supporting part, and a threading hole for a ligature is formed in the separating threading part; the separating threading part is a flat arc-shaped rod; when blood vessel ligation operation is carried out, a ligature penetrates through the threading hole, so that the operation process is smoother. The used separating threading part is a flat arc-shaped rod, and the rod can directly bypass the blood vessel so as to drive the ligature to penetrate through the bottom of the blood vessel. Compared with a traditional mode of using two tweezers, the mode is simpler and more convenient, higher in efficiency and not prone to causing vascular injury.

This invention discloses a non-human primate visual-spatial working memory testing cage. This invention uses a non-human primate visual-spatial working memory testing cage to measure the learning, discrimination, and reversal abilities, or cognitive and memory abilities, of non-human primates, providing a more comprehensive evaluation of changes in animal brain function and behavior. It can provide a model evaluation tool for central nervous system diseases such as Alzheimer's disease and stroke, and offer new methods for disease research and new drug development.

The present invention discloses a standard cutting device for the ex vivo brain tissue of experimental mice, which includes a cutting frame and a bottom plate rotatably arranged inside it. A positioning and adjusting device, an angle adjusting device and a cutting method are provided on the bottom plate. The brain tissue is positioned by the positioning and adjusting device and the optic chiasm stage, so that it is installed and placed at a predetermined position. The brain tissue is rotated to a set angle range by the angle adjusting device in combination with the angle positioning line on the side wall of the cutting frame, and the cutting of the three-dimensional brain tissue can be realized through the slicing seam, obtaining a standard coronal plane of the brain tissue. The present invention combines the morphological characteristics of the animal brain, and through calculation, it can accurately determine the spatial positioning of the animal brain and vertically cut it, ensuring that the subsequent frozen section is matched with the brain coronal section of the standard brain atlas in medical experiments. The overall structure is convenient to operate and low in cost, greatly improving the standardization degree of brain sections.

The invention belongs to the technical field of animal dissection, and innovatively provides a rapid dissection method for the skull of a piglet at the age of about 1 month based on development characteristics of the skull of the piglet aiming at the prominent problems of low separation efficiency, incomplete exposure of a brain structure, high operation injury risk and the like in a traditional dissection method for the skull of the pig. According to the method, a CT image is used for assisting in positioning key nodes of a bone seam, and a dynamic shearing force regulation and control technology of special anatomical bone scissors is combined, so that efficient separation of parietal bones and complete exposure of a brain structure are realized. The time for anatomy of the skull of a single piglet is only 1-2 minutes, the meninx tearing rate is reduced to 0.5% or below, qualitative leap is achieved compared with a traditional method, and a revolutionary technical scheme is provided for large-scale animal brain research and slaughter tests.

The invention discloses a non-human primate animal learning and reversion ability testing method. According to the invention, a standardized test method and a test device are used for measuring the learning discrimination and reversion ability of non-human primates, changes of brain functions and behaviors of animals are evaluated more comprehensively, a model evaluation means can be provided for central nervous system diseases such as Alzheimer's disease and cerebral apoplexy, and a new method is provided for disease research and new drug research.