10 results about "Microdialysis" patented technology

The invention discloses a three-dimensional electric field vector detection and microdialysis synchronous sampling implanted probe system, and belongs to the technical field of neural electrophysiology detection. The system comprises a vector electric field detection module (containing multiple layers of platinum-iridium alloy contacts) and a microdialysis module (provided with a semi-permeable membrane window with high selectivity and biological compatibility) which are coaxially integrated, and spatial isolation and synchronous acquisition of electro-chemical signals are realized through an insulating support structure. The problems of separation and spatial dislocation of an electric field detection and biochemical sampling system in the prior art are solved, real-time association of three-dimensional electric field vector reconstruction and neurotransmitter analysis is supported, and accurate electro-chemical coupling data is provided for nerve regulation and control research.

The invention discloses a dynamic bionic stomach digestion model and application thereof in micro-plastic detection.The dynamic bionic stomach digestion model comprises a box body, a fixing plate, an artificial stomach model, rolling wheels, a pylorus squeezing plate, a pH meter, a heating mechanism, a gastric acid solution cavity, a gastric juice solution cavity, a peristaltic pump and a micro-dialysis probe, and the rolling wheels are rotationally arranged on the fixing plate and located on the periphery of the outer surface of the artificial stomach model; the pylorus extrusion plate is movably arranged on the fixing plate and located on the outer side of the lower end of the artificial stomach model so as to extrude the lower end of the artificial stomach model, and the gastric acid solution cavity and the gastric juice solution cavity are communicated with the upper end of the artificial stomach model through the peristaltic pump. The method comprises the following steps: adding an aged biofilm-loaded micro-plastic sample or a micro-plastic sample loaded with heavy metal and the like and agar balls into an artificial stomach model, digesting in the artificial stomach model, detecting discharged liquid, solids and the like after digestion is completed, detecting the concentration change of digestive juice, micro-plastic particles and micro-plastic precipitates on agar ball particles, and determining the concentration change of the micro-plastic precipitates on the micro-plastic particles and the agar balls. Product identification and the like.

The invention discloses a central obesity early warning method and system based on BDNF dynamic monitoring, and relates to the field of dynamic monitoring of brain-derived neurotrophic factors.The method comprises the steps that the weight, the body length, the body temperature and the food intake of an experimental subject are obtained, fasting blood glucose and blood fat are detected through caudal vein blood sampling, a standardized physiological index data set is formed, and the standard physiological index data set is stored in a database; the method comprises the following steps: carrying out anatomical sampling on hypothalamus tissues, scapular brown adipose and perirenal adipose, and carrying out Western Blot detection to obtain the BDNF expression quantity of the hypothalamus tissues, the BDNF expression quantity of the scapular brown adipose tissues and the BDNF expression quantity of the perirenal adipose tissues; and acquiring the BDNF concentration time sequence data of the hypothalamus extracellular fluid through the implanted microdialysis module. According to the method, dynamic continuous monitoring of hypothalamic BDNF secretion is achieved through the implanted microdialysis technology, the rhythm disorder degree is quantified by calculating the sample entropy value, the limitation of a traditional static detection method is broken through, and early metabolic regulation abnormity can be captured more sensitively.

The invention discloses a method for high-throughput screening of active components of a natural product based on equilibrium dialysis-mass spectrometry, which comprises the following steps: adding a protein solution on one side of an activated and moist 96-hole microdialysis plate, and adding a to-be-detected solution of a traditional Chinese medicine crude extract on the other side of the activated and moist 96-hole microdialysis plate; respectively recovering samples from the upper solution and the lower solution of the incubated 96-hole microdialysis plate, adding methanol, mixing, standing to precipitate denatured protein, centrifuging to remove precipitate, and taking supernate for LC-MS (liquid chromatography-mass spectrometry) analysis; and carrying out characteristic peak annotation through LC-MS data of the supernate, comparing with a standard product database, and carrying out differential analysis and screening to obtain the required active ingredients of the natural product. According to the method for screening the active ingredients of the natural product, the equilibrium dialysis technology, a high-throughput screening device and mass spectrometry are combined, and the efficient, accurate and anti-interference screening effect is achieved.

The biochemical electroencephalogram synchronous monitoring cortical electrode comprises a strip-shaped substrate, a microdialysis part and a plurality of electrode points connected through electrode guide wires, and the electrode points and the microdialysis part are arranged on the same surface of the substrate; the micro-dialysis part comprises a plurality of micro-dialysis tubes, each electrode point corresponds to at least one micro-dialysis tube, the end part of the micro-dialysis tube corresponding to one electrode point is close to the electrode point, the electrode point is used for acquiring an electroencephalogram signal, and the micro-dialysis tubes are used for synchronously and online acquiring biochemical substances to be detected in intercellular fluid. A plurality of electrode points and microdialysis tubes are correspondingly arranged on the same surface of the brain cortex electrode product substrate, the end of each microdialysis tube is close to the corresponding electrode point, the position synchronism of electroencephalogram data acquired by the electrode points and biochemical data acquired by a microdialysis probe is higher, combined diagnosis of a patient is facilitated, and the diagnosis efficiency is improved. And medical staff can observe physiological, pathological and behavioral changes more accurately.

The present invention relates to a device that allows electroencephalographic and biochemical signals in rodent brains to be read using supports for the electrodes and microdialysis cannulas that move rotationally and along three axes, by means of adjustable manual mechanisms and sections that can be extended using air. The mechanism is operated manually by means of slidable elements and has a blocking mechanism and an extension element that extends using air for the vertical electrode insertion movement. The device is also capable of simultaneously inserting microdialysis cannulas to obtain cerebrospinal fluid to subsequently analyse biochemical signals, while also capturing electroencephalographic signals.

The application discloses an intracranial pressure monitoring system and a monitoring method. The probe adopts a coaxial three-layer sleeve structure and integrates pressure sensing, microdialysis and optical sensing functions. The system forms an in-situ calibration field by periodically perfusing the probe with a solution containing pressure-sensitive and stable fluorescent substances. The absolute reference pressure of the calibration field is measured in real time using an optical method, and the original signal of the built-in pressure sensor is read synchronously. The real-time sensitivity and offset parameters of the sensor in the current biological environment are solved in-situ through linear fitting. Finally, these parameters are used to dynamically correct the subsequent continuous monitoring signals of the sensor, and high-precision intracranial pressure values are output. The application effectively solves the long-term drift problem of the implanted sensor caused by biological contamination and aging, realizes long-term, stable and accurate intracranial pressure monitoring, and can integrate metabolite monitoring function.

The utility model discloses a frame body for microdialysis pre-test medicine bottles, which comprises vertical plates on two sides of the frame body, and a first-layer partition plate, a second-layer partition plate, a movable partition plate and a third-layer partition plate which are arranged between the vertical plates, the first-layer partition plate is close to the top of the frame body and is provided with a first limiting hole, and the second-layer partition plate is close to the top of the frame body and is provided with a second limiting hole. The second-layer partition plate is positioned in the middle of the rack body and is provided with a second limiting hole corresponding to the first limiting hole; the movable partition plate is detachably arranged at the position, close to the bottom face of the second-layer partition plate, of the rack body, the third-layer partition plate is arranged at the bottom of the rack body, and a fixing piece is arranged at the position, close to the first limiting hole, of the first-layer partition plate. According to the frame body for the microdialysis pre-test medicine bottle, during microdialysis pre-test, the medicine bottle can be conveniently placed, water bath operation and activation of an experimental probe are facilitated, the medicine bottle can be well fixed and prevented from shaking, operation is convenient, the microdialysis pre-test experiment can be smoothly carried out, and the service life of the microdialysis pre-test medicine bottle is prolonged. And unnecessary loss caused by misoperation is effectively avoided.

A microdialysis system includes: an input device, first and second connecting pipelines and a flow feedback device that includes a connecting needle, a liquid collecting tank, a calibration weighing module, and a flow feedback control display module; the weighing module weighs the liquid in the liquid collecting tank regularly according to conditions and a program set in the display module and transmits a measured liquid weighing value to the display module; the display module performs a difference operation between the current liquid weighing value and the previous liquid weighing value to obtain a weighing sampling difference value, compares the weighing sampling difference value with a preset value to monitor whether the connecting needle outputs liquid normally, and further feeds back a flow state of fluid in the first and second connecting pipelines. In the microdialysis system, the flow state of each connecting pipeline is monitored by weighing the collected liquid.

The present invention relates to a microdialysis device comprising a tube and a duct that are connected by means of a connector, thereby improving substance distribution as well as sample collection and analysis. In addition, the device has an integrated control to regulate the temperature of the samples and thus keep them under optimal conditions. The microdialysis device of the present invention is equipped with a cell that homogenises and provides a laminar flow to better visualise the substances contained, and analyse and monitor the fluid when it is inside the cell using an emitter and receiver, thereby providing accurate data in real time.