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Method for testing plant endogenous hormones

A plant endogenous hormone and plant technology, applied in the field of determination of plant endogenous hormones, can solve the complex and cumbersome extraction and separation and purification process, time-consuming and laborious, difficult to achieve simultaneous determination of multiple plant endogenous hormones, etc., to avoid Effect of extraction and separation purification process

Inactive Publication Date: 2010-06-16
DALIAN NATIONALITIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process of extraction and separation and purification is very complex and cumbersome, time-consuming and labor-intensive, a little improper operation can easily lead to waste of the hormone to be tested in the sample, and it is difficult to achieve simultaneous determination of multiple plant endogenous hormones

Method used

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  • Method for testing plant endogenous hormones
  • Method for testing plant endogenous hormones
  • Method for testing plant endogenous hormones

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Determination of endogenous hormones in poplar tender stems by microdialysis method.

[0031] 1. Materials and methods

[0032] (1) Test materials:

[0033] Populus wutunensis was planted with cuttings in late April 2009. After it grows for about a month, select well-growing plants and conduct microdialysis sampling on the middle and upper parts of the tender stems to obtain the apoplast dialysate and use Shimadzu LC-20AB High performance liquid chromatograph for detection.

[0034] (2) Chromatographic conditions:

[0035] Shimadzu Inertsil ODS-SP (250mm×4.6mm, 5μm) chromatographic column, mobile phase V (methanol): V (ultra-pure water, which contains 0.2% analytical pure phosphoric acid), column temperature is 30℃, flow rate is 0.6mL / min, detection wavelength: GA, 203nm; 6-BA, 273nm; IAA, 214nm; ABA, 263nm. The injection volume is 20μL.

[0036] (3) Preparation of hormone standard solution:

[0037] Accurately weigh the standard gibberellin, cytokinin, indole acetic...

Embodiment 2

[0060] Example 2. Determination of endogenous hormones in the tender stems of Schefflera by microdialysis.

[0061] The determination method is the same as in Example 1. The dialysate chromatogram obtained by microdialysis sampling is attached Figure 7~10 As shown, the different hormone content in each time period is as attached Picture 12 Shown.

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Abstract

The invention provides a method for testing plant endogenous hormones, comprises the following steps of: carrying out microdialysis sampling on a plant body; carrying out sample injection on the acquired dialyzate on a high-performance liquid chromatograph; and simultaneously separating and determining four main plant endogenous hormones: gibberellic acid (GA), cytokinin (6-BA), indolylacetic acid (IAA) and abscisic acid (ABA). Compared with the traditional method for testing the plant endogenous hormones, the invention realizes the intravital test on the plant endogenous hormones and can reduce the damage to the plant body to maximum extent; in addition, the invention can simply and conveniently extract samples, has simple operation, can really reflect the change of the content of the endogenous hormones in the plant body in the vital movement progress and realize the real-time, intravital and on-line test on the plant endogenous hormones.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a method for determining plant endogenous hormones. technical background [0002] Plant Endogenous Hormones refers to trace organic substances synthesized in the plant body, usually transported from the synthesis site to the action site, at low concentrations (below 1μmol / L), which have a significant regulatory effect on the growth and development of plants. Gibberellin (GA), cytokinin (6-BA), indole acetic acid (IAA), and abscisic acid (ABA) are four important endogenous hormones in plants. The content of endogenous hormones in plants is extremely low, the nature is unstable, and the interference of other compounds in the cells, so the determination method must be very sensitive and specific. The traditional method of determining plant endogenous hormones is as follows: first collect the collected plants The material is frozen with liquid nitrogen and stored in a refrigerator...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/36
Inventor 姜波李日红金华姜国斌
Owner DALIAN NATIONALITIES UNIVERSITY
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