Method for testing plant endogenous hormones

A plant endogenous hormone and plant technology, applied in the field of determination of plant endogenous hormones, can solve the complex and cumbersome extraction and separation and purification process, time-consuming and laborious, difficult to achieve simultaneous determination of multiple plant endogenous hormones, etc., to avoid Effect of extraction and separation purification process
CN101738441AInactive Publication Date: 2010-06-16DALIAN NATIONALITIES UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
DALIAN NATIONALITIES UNIVERSITY
Publication Date
2010-06-16
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for testing plant endogenous hormones, comprises the following steps of: carrying out microdialysis sampling on a plant body; carrying out sample injection on the acquired dialyzate on a high-performance liquid chromatograph; and simultaneously separating and determining four main plant endogenous hormones: gibberellic acid (GA), cytokinin (6-BA), indolylacetic acid (IAA) and abscisic acid (ABA). Compared with the traditional method for testing the plant endogenous hormones, the invention realizes the intravital test on the plant endogenous hormones and can reduce the damage to the plant body to maximum extent; in addition, the invention can simply and conveniently extract samples, has simple operation, can really reflect the change of the content of the endogenous hormones in the plant body in the vital movement progress and realize the real-time, intravital and on-line test on the plant endogenous hormones.
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Description

Technical field

[0001] The invention belongs to the field of biotechnology, and specifically relates to a method for determining plant endogenous hormones. technical background

[0002] Plant Endogenous Hormones refers to trace organic substances synthesized in the plant body, usually transported from the synthesis site to the action site, at low concentrations (below 1μmol / L), which have a significant regulatory effect on the growth and development of plants. Gibberellin (GA), cytokinin (6-BA), indole acetic acid (IAA), and abscisic acid (ABA) are four important endogenous hormones in plants. The content of endogenous hormones in plants is extremely low, the nature is unstable, and the interference of other compounds in the cells, so the determination method must be very sensitive and specific. The traditional method of determining plant endogenous hormones is as follows: first collect the collected plants The material is frozen with liquid nitrogen and stored in a refrigerator...

Claims

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